CN102301005A - 用于检测等位基因变体的方法、组合物和试剂盒 - Google Patents

用于检测等位基因变体的方法、组合物和试剂盒 Download PDF

Info

Publication number
CN102301005A
CN102301005A CN2009801557753A CN200980155775A CN102301005A CN 102301005 A CN102301005 A CN 102301005A CN 2009801557753 A CN2009801557753 A CN 2009801557753A CN 200980155775 A CN200980155775 A CN 200980155775A CN 102301005 A CN102301005 A CN 102301005A
Authority
CN
China
Prior art keywords
allele
specific
specific primer
probe
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2009801557753A
Other languages
English (en)
Chinese (zh)
Inventor
C·陈
R·谭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Inc
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Publication of CN102301005A publication Critical patent/CN102301005A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2009801557753A 2008-12-17 2009-12-17 用于检测等位基因变体的方法、组合物和试剂盒 Pending CN102301005A (zh)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US13852108P 2008-12-17 2008-12-17
US61/138,521 2008-12-17
US16423009P 2009-03-27 2009-03-27
US61/164,230 2009-03-27
US18677509P 2009-06-12 2009-06-12
US61/186,775 2009-06-12
US25162309P 2009-10-14 2009-10-14
US61/251,623 2009-10-14
US25350109P 2009-10-20 2009-10-20
US61/253,501 2009-10-20
US25858209P 2009-11-05 2009-11-05
US61/258,582 2009-11-05
PCT/US2009/006652 WO2010077324A2 (en) 2008-12-17 2009-12-17 Methods, compositions, and kits for detecting allelic variants

Publications (1)

Publication Number Publication Date
CN102301005A true CN102301005A (zh) 2011-12-28

Family

ID=42310453

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801557753A Pending CN102301005A (zh) 2008-12-17 2009-12-17 用于检测等位基因变体的方法、组合物和试剂盒

Country Status (6)

Country Link
US (6) US9534255B2 (enExample)
EP (1) EP2376659B1 (enExample)
JP (1) JP2012511927A (enExample)
CN (1) CN102301005A (enExample)
CA (1) CA2747026A1 (enExample)
WO (2) WO2010077324A2 (enExample)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102618646A (zh) * 2012-03-30 2012-08-01 南方医科大学 一种基因拷贝数的定量检测方法
CN104232750A (zh) * 2014-05-30 2014-12-24 嘉兴雅康博医学检验所有限公司 Nras基因突变检测试剂盒
CN104302402A (zh) * 2012-03-16 2015-01-21 生命技术公司 用于评估生物样品的系统和方法
CN104862401A (zh) * 2015-05-29 2015-08-26 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
CN106520919A (zh) * 2016-09-30 2017-03-22 苏州新海生物科技股份有限公司 一种检测目标核酸序列变异体的组合物和方法及试剂盒
CN107577921A (zh) * 2017-08-25 2018-01-12 云壹生物技术（大连）有限公司 一种肿瘤靶向基因测序数据解析方法
CN110438223A (zh) * 2018-05-03 2019-11-12 同济大学苏州研究院 检测Kras基因点突变的引物、探针及其试剂盒与检测方法
CN117025765A (zh) * 2023-02-01 2023-11-10 珠海圣美生物诊断技术有限公司 多重数字pcr检测试剂盒及其检测方法

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3216874A1 (en) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
US8606527B2 (en) * 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
EP2494066B1 (en) * 2009-10-27 2017-04-05 Swift Biosciences, Inc. Bimolecular primers
CN102115782B (zh) * 2009-12-30 2014-06-18 北京雅康博生物科技有限公司 用于定量检测K-ras突变的试剂盒
US20130071844A1 (en) * 2010-03-24 2013-03-21 Toppan Printing Co., Ltd. Method for detecting target base sequence using competitive primer
KR20130113447A (ko) 2010-09-24 2013-10-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 고정된 프라이머들을 이용하여 표적 dna의 직접적인 캡쳐, 증폭 및 서열화
CN103459612A (zh) * 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
GB201100150D0 (en) * 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
US9528157B2 (en) * 2011-01-14 2016-12-27 Genefirst Limited Methods, compositions, and kits for determing the presence/absence of a variant nucleic acid sequence
EP2663654A1 (en) * 2011-01-14 2013-11-20 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
WO2012151560A2 (en) 2011-05-04 2012-11-08 Biocept, Inc. Methods for detecting nucleic acid sequence variants
CN102367478B (zh) * 2011-10-25 2013-07-31 浙江大学 用于KRAS基因突变分型的ARMS-qPCR检测试剂盒及检测方法
US9738935B2 (en) * 2011-11-10 2017-08-22 Roche Molecular Systems, Inc. Complex mutations in the epidermal growth factor receptor kinase domain
EP4361607A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
JP6108407B2 (ja) * 2012-03-15 2017-04-05 公益財団法人ヒューマンサイエンス振興財団 マクロライド系抗生物質耐性変異菌の検出方法及び検出キット
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP2749654A1 (en) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analysis of composition of nucleic acid mixtures
ES2662825T3 (es) * 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
KR20160106041A (ko) * 2013-08-14 2016-09-09 퀴아젠 맨스필드, 인코퍼레이티드 Ｎｒａｓ 및 ｂｒａｆ 핵산의 멀티플렉스 분석을 위한 조성물 및 방법
CA2939124C (en) 2014-02-07 2023-08-29 University Of Iowa Research Foundation Oligonucleotide-based probes and methods for detection of microbes
US10329605B2 (en) * 2015-04-20 2019-06-25 Neogenomics Laboratories, Inc. Method to increase sensitivity of detection of low-occurrence mutations
CN104762410A (zh) * 2015-04-29 2015-07-08 上海允英医疗科技有限公司 用于全ras突变检测的引物、探针及检测试剂盒
US9657353B2 (en) 2015-06-26 2017-05-23 Great Basin Scientific, Inc. Specific detection of organisms derived from a sample
CN107022607B (zh) * 2017-03-16 2019-01-22 北京博奥晶典生物技术有限公司 通过碱基错配解决多重pcr时等位基因扩增不平衡的方法
JP7722675B2 (ja) * 2017-05-16 2025-08-13 公立大学法人和歌山県立医科大学 Ｅｇｆｒ変異非小細胞肺がんにおけるｅｇｆｒチロシンキナーゼ阻害剤の治療奏功性の予測方法
JP7421202B2 (ja) 2017-09-29 2024-01-24 ユニバーシティー オブ アイオワ リサーチ ファンデーション デジタルヌクレアーゼ検出組成物および方法
EP3781710A4 (en) 2018-04-17 2022-01-05 Chromacode, Inc. METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US20210189473A1 (en) * 2018-09-06 2021-06-24 ChromaCode,Inc. Universal tail primers with multiple binding motifs for multiplexed detection of single nucleotide polymorphisms

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
EP0472648A4 (en) 1989-05-18 1992-09-16 Microprobe Corporation Crosslinking oligonucleotides
US5451463A (en) 1989-08-28 1995-09-19 Clontech Laboratories, Inc. Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5419966A (en) 1991-06-10 1995-05-30 Microprobe Corporation Solid support for synthesis of 3'-tailed oligonucleotides
WO1993001315A1 (en) * 1991-07-01 1993-01-21 The Blood Center Of Southeastern Wisconsin PEN POLYMORPHISM OF HUMAN PLATELET MEMBRANE GLYCOPROTEIN IIIa AND DIAGNOSTIC AND THERAPEUTIC APPLICATION THEREOF
US5512677A (en) 1991-08-13 1996-04-30 National Science Council 3-substituted methyl-2,3-dihydroimidazo 1,2-c!quinazoline derivatives, the preparation and use thereof
GB9211979D0 (en) 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU698953B2 (en) 1994-04-29 1998-11-12 Applied Biosystems, Llc System for real time detection of nucleic acid amplification products
US5512441A (en) * 1994-11-15 1996-04-30 American Health Foundation Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5736626A (en) 1996-01-29 1998-04-07 The Perkin-Elmer Corporation Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
DE69738687D1 (de) 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
AU726501B2 (en) 1996-06-04 2000-11-09 University Of Utah Research Foundation Monitoring hybridization during PCR
US5861295A (en) 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6043070A (en) 1997-08-29 2000-03-28 The Perkin-Elmer Corporation Phosphoramidate-phosphodiester oligonucleotide chimera as primers
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
EP1025120B1 (en) 1997-10-27 2010-08-18 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
US6383752B1 (en) 1999-03-31 2002-05-07 Hybridon, Inc. Pseudo-cyclic oligonucleobases
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US20030082549A1 (en) 2000-08-30 2003-05-01 Xiangjun Liu Method for determining alleles
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6391592B1 (en) 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
DE10112515B4 (de) 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6589250B2 (en) 2001-11-20 2003-07-08 Stephen A. Schendel Maxillary distraction device
US7253007B2 (en) 2001-11-30 2007-08-07 Chemocentryx, Inc. Compositions and methods for detecting and treating diseases and conditions related to chemokine receptors
WO2003050304A1 (en) 2001-12-08 2003-06-19 Seegene, Inc Annealing control primer system for regulating primer annealing specificity and its applications
NZ532531A (en) 2001-12-08 2007-01-26 Seegene Inc Annealing control primer for improving annealing specificity in nucleic acid amplification
US7228237B2 (en) 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
EP2031070B1 (en) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplex amplification of polynucleotides
US7348146B2 (en) 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
US20050287553A1 (en) 2004-04-06 2005-12-29 Epigenomics Ag Method for the quantification of methylated DNA
US7563572B2 (en) 2004-08-02 2009-07-21 University Of Utah Research Foundation Method for long range allele-specific PCR
DE102005011398A1 (de) 2005-03-03 2006-09-14 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA
EP2477029A1 (en) 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US9797005B2 (en) 2005-11-23 2017-10-24 University Of Southern California High throughput method of DNA methylation haplotyping
WO2007106534A2 (en) * 2006-03-14 2007-09-20 Harbor-Ucla Research And Education Institute Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
WO2008111990A1 (en) 2006-06-14 2008-09-18 Cellpoint Diagnostics, Inc. Rare cell analysis using sample splitting and dna tags
EP1905842A1 (en) * 2006-09-26 2008-04-02 DiaSorin S.p.A. Method for detection of mutant alleles combining Real Time PCR and REMS-PCR
GB0703996D0 (en) 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
US20100041048A1 (en) 2008-07-31 2010-02-18 The Johns Hopkins University Circulating Mutant DNA to Assess Tumor Dynamics
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
EP3249053A1 (en) 2009-03-27 2017-11-29 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US20110287424A1 (en) 2009-03-27 2011-11-24 Life Technologies Corporation Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr)
EP2576832B1 (en) 2010-05-25 2016-05-04 The Johns Hopkins University Compositions and methods for detecting a neoplasia
EP2400035A1 (en) 2010-06-28 2011-12-28 Technische Universität München Methods and compositions for diagnosing gastrointestinal stromal tumors
EP2663654A1 (en) 2011-01-14 2013-11-20 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
WO2012151560A2 (en) 2011-05-04 2012-11-08 Biocept, Inc. Methods for detecting nucleic acid sequence variants
WO2014004726A1 (en) 2012-06-26 2014-01-03 Caifu Chen Methods, compositions and kits for the diagnosis, prognosis and monitoring of cancer

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AFONINA I ET AL.: "Sequence-specific arrest of primer extension on single-stranded DNA by an oligonucleotide-minor groove binder conjugate", 《PNAS》 *
JOHNSON MP ET AL.: "Locked nucleic acid(LNA) single nucleotide polymorphism(SNP) genotype analysis and validation using real-time PCR", 《NUCLEIC ACIDS RES》 *
PARSONS BL AND HEFLICH RH: "Detection of a mouse H-ras codon 61 mutation using a modified allele-specific competetitive blocnker pcr genotypic selection method", 《MUTAGENESIS》 *
SEELA F ET AL: "The N8-(2’-deoxyribofuranoside) of 8-aza-7-deazaadenine:a universal nucleoside forming specific hydrogen bonds with the four canonical constituents", 《NUCLEIC ACID RES》 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11987840B2 (en) 2012-03-16 2024-05-21 Life Technologi s Corporation Systems and methods for assessing of biological samples
CN104302402A (zh) * 2012-03-16 2015-01-21 生命技术公司 用于评估生物样品的系统和方法
CN104302402B (zh) * 2012-03-16 2017-07-04 生命技术公司 用于评估生物样品的系统和方法
CN102618646B (zh) * 2012-03-30 2014-08-20 南方医科大学 一种基因拷贝数的定量检测方法
CN102618646A (zh) * 2012-03-30 2012-08-01 南方医科大学 一种基因拷贝数的定量检测方法
CN104232750A (zh) * 2014-05-30 2014-12-24 嘉兴雅康博医学检验所有限公司 Nras基因突变检测试剂盒
WO2015180489A1 (zh) * 2014-05-30 2015-12-03 嘉兴雅康博医学检验所有限公司 Nras基因突变检测试剂盒
CN104862401A (zh) * 2015-05-29 2015-08-26 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
CN106520919A (zh) * 2016-09-30 2017-03-22 苏州新海生物科技股份有限公司 一种检测目标核酸序列变异体的组合物和方法及试剂盒
WO2018059525A1 (zh) * 2016-09-30 2018-04-05 苏州新海生物科技股份有限公司 一种检测目标核酸序列变异体的组合物和方法及试剂盒
CN107577921A (zh) * 2017-08-25 2018-01-12 云壹生物技术（大连）有限公司 一种肿瘤靶向基因测序数据解析方法
CN110438223A (zh) * 2018-05-03 2019-11-12 同济大学苏州研究院 检测Kras基因点突变的引物、探针及其试剂盒与检测方法
CN110438223B (zh) * 2018-05-03 2023-03-14 同济大学苏州研究院 检测Kras基因点突变的引物、探针及其试剂盒与检测方法
CN117025765A (zh) * 2023-02-01 2023-11-10 珠海圣美生物诊断技术有限公司 多重数字pcr检测试剂盒及其检测方法

Also Published As

Publication number Publication date
US20160040256A1 (en) 2016-02-11
JP2012511927A (ja) 2012-05-31
WO2010077324A2 (en) 2010-07-08
US20100221717A1 (en) 2010-09-02
WO2010080559A2 (en) 2010-07-15
US20180208996A1 (en) 2018-07-26
EP2376659B1 (en) 2015-12-02
US20240132946A1 (en) 2024-04-25
EP2376659A4 (en) 2013-01-23
EP2376659A2 (en) 2011-10-19
US20200255907A1 (en) 2020-08-13
WO2010080559A3 (en) 2010-10-28
WO2010077324A3 (en) 2010-10-28
US10570459B2 (en) 2020-02-25
CA2747026A1 (en) 2010-07-08
US9534255B2 (en) 2017-01-03
US11530450B2 (en) 2022-12-20
US20230323472A1 (en) 2023-10-12

Similar Documents

Publication Publication Date Title
CN102428190B (zh) 用于检测等位基因变体的方法、组合物和试剂盒
CN102301005A (zh) 用于检测等位基因变体的方法、组合物和试剂盒
US9512482B2 (en) Compositions and methods for detecting allelic variants
US20120214160A1 (en) Methods, compositions, and kits for detecting rare cells
CN116064747A (zh) 用于变体检测的方法
WO2011139920A2 (en) Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr)
CN115135776A (zh) 多重drop-off数字聚合酶链式反应方法
US20210324461A1 (en) Reagents, mixtures, kits and methods for amplification of nucleic acids
HK1162195A (en) Methods, compositions, and kits for detecting allelic variants

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1162195

Country of ref document: HK

RJ01 Rejection of invention patent application after publication

Application publication date: 20111228

RJ01 Rejection of invention patent application after publication