JP2011514163A5 - - Google Patents

Download PDF

Info

Publication number
JP2011514163A5
JP2011514163A5 JP2010550908A JP2010550908A JP2011514163A5 JP 2011514163 A5 JP2011514163 A5 JP 2011514163A5 JP 2010550908 A JP2010550908 A JP 2010550908A JP 2010550908 A JP2010550908 A JP 2010550908A JP 2011514163 A5 JP2011514163 A5 JP 2011514163A5
Authority
JP
Japan
Prior art keywords
probe
analyte
nucleic acid
target nucleic
specific portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2010550908A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011514163A (ja
JP5651022B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2009/037194 external-priority patent/WO2009117327A2/en
Publication of JP2011514163A publication Critical patent/JP2011514163A/ja
Publication of JP2011514163A5 publication Critical patent/JP2011514163A5/ja
Application granted granted Critical
Publication of JP5651022B2 publication Critical patent/JP5651022B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2010550908A 2008-03-15 2009-03-13 増幅反応中の核酸分子を分析するための組成物及び方法 Active JP5651022B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3695308P 2008-03-15 2008-03-15
US61/036,953 2008-03-15
PCT/US2009/037194 WO2009117327A2 (en) 2008-03-15 2009-03-13 Compositions and methods for analysis of nucleic acid molecules during amplification reactions

Publications (3)

Publication Number Publication Date
JP2011514163A JP2011514163A (ja) 2011-05-06
JP2011514163A5 true JP2011514163A5 (enExample) 2012-04-19
JP5651022B2 JP5651022B2 (ja) 2015-01-07

Family

ID=41091477

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010550908A Active JP5651022B2 (ja) 2008-03-15 2009-03-13 増幅反応中の核酸分子を分析するための組成物及び方法

Country Status (11)

Country Link
US (2) US20090253142A1 (enExample)
EP (1) EP2274448B1 (enExample)
JP (1) JP5651022B2 (enExample)
KR (1) KR101419980B1 (enExample)
CN (1) CN102301000B (enExample)
AU (1) AU2009225835B2 (enExample)
BR (1) BRPI0908748A2 (enExample)
CA (1) CA2718011C (enExample)
IL (1) IL208036A (enExample)
MX (1) MX2010010161A (enExample)
WO (1) WO2009117327A2 (enExample)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151457A1 (en) * 2009-12-22 2011-06-23 Elitech Holding B.V. Hypertheromostable endonuclease iv substrate probe
US20120107808A1 (en) * 2010-10-27 2012-05-03 Weiwei Li High throughput detection of gene-specific hydroxymethylation
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
US8916344B2 (en) * 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8361720B2 (en) * 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US9493824B2 (en) 2010-12-16 2016-11-15 Bio-Rad Laboratories, Inc. Universal reference dye for quantitative amplification
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
WO2012106525A2 (en) 2011-02-02 2012-08-09 Exact Sciences Corporation Digital sequence analysis of dna methylation
JP5852222B2 (ja) 2011-03-29 2016-02-03 シージーン アイエヌシー Pto切断及び延長−依存的切断によるターゲット核酸配列の検出
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
US8993341B2 (en) 2011-05-12 2015-03-31 Exact Sciences Corporation Removal of PCR inhibitors
CN103649298A (zh) 2011-05-12 2014-03-19 精密科学公司 核酸的分离
US8808990B2 (en) 2011-05-12 2014-08-19 Exact Sciences Corporation Serial isolation of multiple DNA targets from stool
CA2840542A1 (en) * 2011-06-28 2013-01-03 Igor Kutyavin Methods and compositions for enrichment of nucleic acids in mixtures of highly homologous sequences
US9127318B2 (en) 2011-10-18 2015-09-08 Exact Sciences Corporation Multiplexed KRAS mutation detection assay
DE102011055247A1 (de) * 2011-11-10 2013-05-16 Albert-Ludwigs-Universität Freiburg Multianalyt-Reportersystem
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Pto 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
CA2864523C (en) 2012-03-05 2019-02-05 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
US9710596B2 (en) 2012-11-21 2017-07-18 Exact Sciences Corporation Methods for quantifying nucleic acid variations
WO2014158628A1 (en) 2013-03-14 2014-10-02 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules
CA2902916C (en) 2013-03-14 2018-08-28 Mayo Foundation For Medical Education And Research Detecting neoplasm
US10253358B2 (en) 2013-11-04 2019-04-09 Exact Sciences Development Company, Llc Multiple-control calibrators for DNA quantitation
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
EP3084004A4 (en) 2013-12-19 2017-08-16 Exact Sciences Corporation Synthetic nucleic acid control molecules
KR101598398B1 (ko) 2014-01-08 2016-02-29 (주)제노텍 5''-플랩 엔도뉴클레이즈 활성의 억제를 이용하여 실시간 중합효소 연쇄반응으로 돌연변이 유전자를 검사하는 방법
ES2812753T3 (es) 2014-03-31 2021-03-18 Mayo Found Medical Education & Res Detección de neoplasma colorectal
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
CN113981057A (zh) 2014-12-12 2022-01-28 精密科学公司 用于进行甲基化检测测定的组合物和方法
EP3230476B1 (en) 2014-12-12 2020-02-05 Exact Sciences Development Company, LLC Zdhhc1 for normalizing methylation detection assays
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
DE102015115836A1 (de) 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre Nukleinsäure-Amplifikate in einem kontinuierlichen Reaktionsansatz und unmittelbare Auswertung mittels immunchromatographischer Verfahren
WO2017046192A1 (de) * 2015-09-18 2017-03-23 Biotype Diagnostic Gmbh Bestätigungstest für primäre nukleinsäure-amplifikate in einem kontinuierlichen reaktionsansatz und unmittelbare auswertung mittels elektrophoretischer verfahren
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
WO2017192221A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by analysis of methylated dna
ES2902174T3 (es) 2016-07-19 2022-03-25 Exact Sciences Dev Co Llc ADN de control metilado
EP3488003B1 (en) 2016-07-19 2023-10-25 Exact Sciences Corporation Nucleic acid control molecules from non-human organisms
WO2018045322A1 (en) 2016-09-02 2018-03-08 Mayo Foundation For Medical Education And Research Detecting hepatocellular carcinoma
CN110475875B (zh) 2017-01-27 2024-06-25 精密科学公司 通过分析甲基化dna检测结肠瘤形成
CA3054836A1 (en) 2017-02-28 2018-09-07 Mayo Foundation For Medical Education And Research Detecting prostate cancer
EP4491721A3 (en) 2017-05-19 2025-03-05 Gen-Probe Incorporated Dried compositions containing flap endonuclease
AU2018300000B2 (en) 2017-07-10 2021-11-11 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
EP3663410A4 (en) * 2017-07-31 2020-07-08 Toppan Printing Co., Ltd. NUCLEIC ACID DETECTION METHOD AND DETECTION REAGENT
WO2019032809A1 (en) 2017-08-11 2019-02-14 Gen-Probe Incorporated COMPOSITIONS AND METHODS FOR DETECTING STAPHYLOCOCCUS AUREUS
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
CN111655869B (zh) 2017-11-30 2024-06-28 梅约医学教育与研究基金会 检测乳腺癌
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
US11662281B2 (en) 2017-12-13 2023-05-30 Gen-Probe Incorporated Compositions and methods for biological sample processing
EP3724354A1 (en) 2017-12-15 2020-10-21 Gen-Probe Incorporated Compositions and methods for detecting toxigenic clostridium difficile
DK3746225T3 (da) 2018-01-29 2025-01-13 Gen Probe Inc Analytiske systemer og fremgangsmåder
EP4647508A2 (en) 2018-04-02 2025-11-12 Enumera Molecular, Inc. Methods, systems, and compositions for counting nucleic acid molecules
US20210317515A1 (en) 2018-07-10 2021-10-14 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
DE102018213026A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Verfahren zur Durchführung einer Echtzeit-PCR
US11408030B2 (en) 2018-09-10 2022-08-09 Andy Madrid Test for detecting Alzheimer's disease
AU2020210753A1 (en) 2019-01-25 2021-09-02 Gen-Probe Incorporated Detection of drug-resistant mycoplasma genitalium
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
US12210028B2 (en) 2019-05-03 2025-01-28 Gen-Probe Incorporated Receptacle delivery system
US11427874B1 (en) 2019-08-26 2022-08-30 Epi One Inc. Methods and systems for detection of prostate cancer by DNA methylation analysis
CN114651070A (zh) 2019-09-16 2022-06-21 精密科学公司 结构和温度依赖性瓣状核酸内切酶底物
KR20220092561A (ko) 2019-10-31 2022-07-01 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 난소암 검출
CN115605138A (zh) 2020-04-17 2023-01-13 精密科学公司(Us) 可摄取取样装置
CN111662804A (zh) * 2020-06-17 2020-09-15 清华大学 用于食品微生物检测的数字环介导等温扩增的微流控芯片
EP4200445A4 (en) 2020-08-19 2025-08-27 Mayo Found Medical Education & Res DETECTION OF NON-HODGKIN'S LYMPHOMA
CA3195721A1 (en) 2020-09-21 2022-03-24 Progenity, Inc. Compositions and methods for isolation of cell-free dna
EP4232379A2 (en) 2020-10-21 2023-08-30 Gen-Probe Incorporated Fluid container management system
WO2022140581A1 (en) * 2020-12-23 2022-06-30 New York University Methods and compositions for measuring and inhibiting exonuclease activity
CN113512767B (zh) * 2021-04-30 2022-09-23 天津诺禾致源生物信息科技有限公司 用于构建small RNA文库的接头、试剂盒及small RNA文库的构建方法
US20220372564A1 (en) * 2021-05-21 2022-11-24 Tetracore, Inc. Detection of recombinase polymerase amplification (rpa) amplicons via fen1 cleavage
WO2023009842A1 (en) * 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
EP4314286A1 (en) 2021-08-06 2024-02-07 Sansure Biotech Inc. Compositions for liquefying a viscous biological sample, combination products, liquefying agents, and kits thereof, and methods and application thereof
JP7641445B2 (ja) 2021-09-30 2025-03-06 ジェン-プローブ・インコーポレーテッド 温度で選択可能なfretカセットシグナル伝達
CN114134219A (zh) * 2021-12-14 2022-03-04 广州市金圻睿生物科技有限责任公司 一种多重核酸检测系统及其制备方法与应用
JP2024000312A (ja) * 2022-06-20 2024-01-05 Toppanホールディングス株式会社 蛍光物質及び消光物質で標識された一本鎖オリゴヌクレオチド
WO2024054924A1 (en) 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers
CN115807131A (zh) * 2022-12-21 2023-03-17 中国人民解放军东部战区总医院 一种用于rna病毒核酸的闭管可视化检测方法
KR102678676B1 (ko) * 2023-03-02 2024-06-26 강경찬 인공핵산을 이용한 표적핵산의 검출 방법
WO2024186140A1 (ko) * 2023-03-07 2024-09-12 강경찬 인공핵산을 이용한 표적핵산의 검출 방법

Family Cites Families (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6014A (en) * 1849-01-09 Stop-motion for drawing-frames
US6011A (en) * 1849-01-09 Tooth-extractor
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) * 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4818680A (en) * 1985-12-18 1989-04-04 Mary Collins Method and kit involving displacement and rehybridization of labeled polynucleotide
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5030557A (en) * 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
US5403711A (en) * 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
DE68911648T2 (de) 1988-03-24 1994-06-23 Univ Iowa Res Found Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird.
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5149798A (en) * 1989-04-06 1992-09-22 Worcester Foundation For Experimental Biology Process for synthesizing oligonucleotides and their analogs adaptable to large scale syntheses
DE69022180T2 (de) 1989-05-31 1996-02-01 Amoco Corp Universelle nukleinsäuresonden für eubakterien sowie methoden.
US5925525A (en) * 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US5144019A (en) * 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
SG46627A1 (en) 1989-12-22 1998-02-20 Hoffmann La Roche Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5849481A (en) * 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5177196A (en) * 1990-08-16 1993-01-05 Microprobe Corporation Oligo (α-arabinofuranosyl nucleotides) and α-arabinofuranosyl precursors thereof
ES2134198T3 (es) 1990-09-28 1999-10-01 Hoffmann La Roche Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas.
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5714331A (en) * 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5766855A (en) * 1991-05-24 1998-06-16 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity and sequence specificity
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5248618A (en) * 1991-06-05 1993-09-28 Life Technologies, Inc. Methods for generating light with chemiluminescent dioxetanes activated by anchimeric assisted cleavage
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US7045289B2 (en) 1991-09-09 2006-05-16 Third Wave Technologies, Inc. Detection of RNA Sequences
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7150982B2 (en) 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
CA2124796A1 (en) * 1991-12-23 1993-07-08 Chiron Diagnostics Corporation Cmv probes for use in solution phase sandwich hybridization assays
EP0590327B1 (en) * 1992-09-11 2003-04-09 F. Hoffmann-La Roche Ag Detection of nucleic acids in blood
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US6673616B1 (en) 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5888780A (en) * 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
EP0601889A2 (en) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nucleic acid probes
US5858659A (en) * 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US6045996A (en) * 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US5851770A (en) * 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
CA2188660C (en) * 1994-04-25 2005-01-18 Richard G. H. Cotton Detection of mutation by resolvase cleavage
US6169169B1 (en) * 1994-05-19 2001-01-02 Dako A/S PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis
ATE206764T1 (de) * 1994-07-16 2001-10-15 Roche Diagnostics Gmbh Verfahren zum sensitiven nachweis von nukleinsäuren
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5571673A (en) * 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5545729A (en) * 1994-12-22 1996-08-13 Hybridon, Inc. Stabilized ribozyme analogs
EP1260593A3 (en) * 1994-12-23 2003-12-03 Dade Behring Inc. Detection of nucleic acids by nuclease-catalyzed product formation
US5874283A (en) * 1995-05-30 1999-02-23 John Joseph Harrington Mammalian flap-specific endonuclease
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5968740A (en) * 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US5912340A (en) 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1997023647A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Homogeneous amplification and detection of nucleic acids
US7195871B2 (en) 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US7122364B1 (en) * 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US7256020B2 (en) 1996-11-29 2007-08-14 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
CA2243353C (en) 1996-01-24 2010-03-30 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7432048B2 (en) 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US5719056A (en) * 1996-04-24 1998-02-17 Amersham Life Science, Inc. Proteins from pyrococcus furiosus
EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
ATE530652T1 (de) 1996-11-29 2011-11-15 Third Wave Tech Inc Fen-1-endonucleasen, mischungen und spaltungsverfahren
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US6001311A (en) * 1997-02-05 1999-12-14 Protogene Laboratories, Inc. Apparatus for diverse chemical synthesis using two-dimensional array
WO1998044161A1 (en) 1997-04-03 1998-10-08 Life Technologies, Inc. Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)
JP2001523111A (ja) 1997-05-05 2001-11-20 サード ウェーブ テクノロジーズ,インコーポレーテッド 構造体架橋オリゴヌクレオチドを使用した標的依存型反応
US6214545B1 (en) 1997-05-05 2001-04-10 Third Wave Technologies, Inc Polymorphism analysis by nucleic acid structure probing
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6013459A (en) * 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
US6063573A (en) * 1998-01-27 2000-05-16 Clinical Micro Sensors, Inc. Cycling probe technology using electron transfer detection
WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6365376B1 (en) 1999-02-19 2002-04-02 E. I. Du Pont De Nemours And Company Genes and enzymes for the production of adipic acid intermediates
US6255476B1 (en) 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
JP4470275B2 (ja) 2000-04-27 2010-06-02 株式会社島津製作所 核酸合成法
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
JP2004521606A (ja) 2000-05-24 2004-07-22 サード・ウェーブ・テクノロジーズ・インク Rnaの検出法
JP2001352982A (ja) 2000-06-12 2001-12-25 Shimadzu Corp 核酸合成法
US7060436B2 (en) 2000-06-17 2006-06-13 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6855553B1 (en) 2000-10-02 2005-02-15 3M Innovative Properties Company Sample processing apparatus, methods and systems
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
AU2001297524A1 (en) 2000-11-15 2002-09-19 Third Wave Technologies, Inc. Fen endonucleases
US7309573B2 (en) * 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7435390B2 (en) 2001-01-26 2008-10-14 Third Wave Technologies, Inc. Nucleic acid synthesizers
US6932943B1 (en) 2001-01-26 2005-08-23 Third Wave Technologies Nucleic acid synthesizers
WO2002090572A2 (en) 2001-05-09 2002-11-14 Third Wave Technologies, Inc. Nucleic acid detection in pooled samples
US7294759B2 (en) 2001-06-29 2007-11-13 E. I. Du Pont De Nemours And Company Alteration of oil traits in plants
US7303869B2 (en) 2001-07-17 2007-12-04 Northwestern University Solid-phase reactions
US20030082547A1 (en) * 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
US6783940B2 (en) 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR
US7482118B2 (en) 2001-11-15 2009-01-27 Third Wave Technologies, Inc. Endonuclease-substrate complexes
DE10219994B4 (de) 2002-05-03 2005-09-29 Filtertek B.V. Einweg-Ventileinrichtung
US7312033B2 (en) 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
US7297780B2 (en) 2003-01-06 2007-11-20 Third Wave Technologies, Inc. Reactive functional groups for preparation of modified nucleic acid
AU2004275865B2 (en) 2003-09-25 2008-09-25 Third Wave Technologies, Inc. Detection of HPV
CA2552949C (en) 2004-01-07 2012-10-02 Third Wave Technologies, Inc. Determination of hepatitis c virus genotype
US7462451B2 (en) 2004-04-26 2008-12-09 Third Wave Technologies, Inc. Compositions for modifying nucleic acids
WO2006050499A2 (en) * 2004-11-03 2006-05-11 Third Wave Technologies, Inc. Single step detection assay
US7482127B2 (en) 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides

Similar Documents

Publication Publication Date Title
JP2011514163A5 (enExample)
AU2018204665B2 (en) Detection of target nucleic acid sequences using different detection temperatures
CN104145029B (zh) 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测
CN107208134B (zh) 使用不同检测温度及基准值的靶核酸序列检测
JP2019528726A5 (enExample)
JP5770738B2 (ja) Thdプライマーターゲット検出
JP2019532628A5 (enExample)
CN103228797B (zh) 利用双重标记固定化探针的固相中的靶核酸序列检测
KR20170003695A (ko) 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭
RU2012142160A (ru) Детекция нуклеиновокислотных последовательностей-мишеней в анализе с расщеплением и удлинением рто
JP2010539920A5 (enExample)
JP6796660B2 (ja) 切断された相補的なタグ切片を用いた標的核酸配列の検出方法及びその組成物
JP2013509871A5 (enExample)
JP7737474B2 (ja) 複数の検出温度を使用する複数の標的核酸の検出
CN107208139A (zh) 与靶核酸序列有关的信号的分辨
CN100577816C (zh) 一种荧光标记的寡核苷酸探针及其应用
CN104164506A (zh) 实时pcr检测单核苷酸多态性的方法及其应用
JP2016527904A5 (enExample)
US7964716B2 (en) Fluorescent primer system for detection of nucleic acids (Q priming)
EP4381097A1 (en) Methods, systems and compositions for detection of multiple analytes
KR20110048734A (ko) 5―미스매치 프로브 타겟 검출
CN105063190A (zh) 微小rna的固相芯片恒温检测方法
US20180251821A1 (en) Detection method and detection kit for nucleic acid molecules
KR102253767B1 (ko) 교차 핵산분해 반응을 이용한 snp 검출 방법
RU2414510C2 (ru) Способ амплификации специфичных фрагментов нуклеиновых кислот с помощью рекуррентной цепной реакции