JP2019532628A5 - - Google Patents
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- JP2019532628A5 JP2019532628A5 JP2019514294A JP2019514294A JP2019532628A5 JP 2019532628 A5 JP2019532628 A5 JP 2019532628A5 JP 2019514294 A JP2019514294 A JP 2019514294A JP 2019514294 A JP2019514294 A JP 2019514294A JP 2019532628 A5 JP2019532628 A5 JP 2019532628A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- oligonucleotide
- quenching
- component
- tag portion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662395325P | 2016-09-15 | 2016-09-15 | |
| US62/395,325 | 2016-09-15 | ||
| US201662435595P | 2016-12-16 | 2016-12-16 | |
| US62/435,595 | 2016-12-16 | ||
| US201762536871P | 2017-07-25 | 2017-07-25 | |
| US62/536,871 | 2017-07-25 | ||
| PCT/EP2017/073288 WO2018050824A1 (en) | 2016-09-15 | 2017-09-15 | Methods for performing multiplexed pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019532628A JP2019532628A (ja) | 2019-11-14 |
| JP2019532628A5 true JP2019532628A5 (enExample) | 2020-10-22 |
| JP7221199B2 JP7221199B2 (ja) | 2023-02-20 |
Family
ID=59901522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019514212A Active JP7063886B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスリアルタイムｐｃｒを実施する方法 |
| JP2019514294A Active JP7221199B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスｐｃｒの実施方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019514212A Active JP7063886B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスリアルタイムｐｃｒを実施する方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US11028433B2 (enExample) |
| EP (2) | EP3512959B1 (enExample) |
| JP (2) | JP7063886B2 (enExample) |
| KR (2) | KR102468633B1 (enExample) |
| CN (2) | CN109689889A (enExample) |
| AU (2) | AU2017328617B2 (enExample) |
| CA (2) | CA3036119C (enExample) |
| DK (1) | DK3512959T3 (enExample) |
| ES (2) | ES2893411T3 (enExample) |
| WO (2) | WO2018050828A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2017328617B2 (en) | 2016-09-15 | 2023-06-29 | F. Hoffmann-La Roche Ag | Methods for performing multiplexed real-time PCR |
| RU2665631C2 (ru) * | 2017-01-25 | 2018-09-03 | Общество с ограниченной ответственностью "Секвойя Дженетикс" | Способ специфической идентификации последовательностей днк |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| CN111629831A (zh) | 2018-01-22 | 2020-09-04 | 卢米耐克斯公司 | 用于离散解链分析的方法和组合物 |
| US20190292596A1 (en) | 2018-03-21 | 2019-09-26 | Roche Molecular Systems, Inc. | Modified nucleoside phosphates with high thermal stability |
| AU2019255933B2 (en) | 2018-04-20 | 2022-08-25 | Seegene, Inc. . | Method and apparatus for detecting a plurality of target nucleic acid sequences in sample |
| US11384376B2 (en) | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| EP3833778A4 (en) * | 2018-08-09 | 2022-12-07 | SpeeDx Pty Ltd | MULTIPLEX DETECTION OF NUCLEIC ACIDS |
| US11371080B2 (en) | 2018-11-30 | 2022-06-28 | Luminex Corporation | Methods and probes for performing PCR with melt analysis for increased multiplexing |
| US20220298555A1 (en) * | 2018-12-20 | 2022-09-22 | Roche Molecular Systems, Inc. | Detection of target nucleic acid by solid-phase molography |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| EP4073175A1 (en) | 2019-12-09 | 2022-10-19 | F. Hoffmann-La Roche AG | Dicationic fluorescent dyes |
| CN111534518B (zh) * | 2020-05-18 | 2021-07-23 | 纳昂达(南京)生物科技有限公司 | 通用封闭序列及其应用 |
| AU2020256848A1 (en) * | 2020-06-30 | 2022-10-27 | Speedx Pty Ltd | Multiplex detection of nucleic acids using mixed reporters |
| CN112195221B (zh) * | 2020-09-13 | 2021-08-06 | 武汉纳诺德医疗科技有限公司 | 一种多温度单荧光通道多重实时荧光定量pcr仪及其检测方法 |
| JP2023541657A (ja) * | 2020-09-16 | 2023-10-03 | ナノストリング テクノロジーズ，インコーポレイティド | 化学組成物およびその使用方法 |
| EP4267764A1 (en) | 2020-12-22 | 2023-11-01 | F. Hoffmann-La Roche AG | Methods for performing multiplexed real-time pcr with the use of large stokes shift fluorescent dyes |
| EP4359561A1 (en) | 2021-06-25 | 2024-05-01 | F. Hoffmann-La Roche AG | Methods for performing temperature multiplexed pcr with increased sensitivity |
| CN119614682A (zh) * | 2021-09-30 | 2025-03-14 | 简·探针公司 | 可温度选择的fret盒信号传导 |
| EP4547757A2 (en) | 2022-06-28 | 2025-05-07 | F. Hoffmann-La Roche AG | Fluorescent dyes with large stokes shift |
| EP4621068A1 (en) * | 2022-11-16 | 2025-09-24 | Seegene, Inc. | Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay |
| AR132405A1 (es) * | 2023-04-19 | 2025-06-25 | Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet | Métodos y sistemas para seleccionar biomarcadores basados en metilación del adn para la detección de cáncer colorrectal |
| AU2024220029A1 (en) * | 2023-05-15 | 2024-11-28 | Speedx Pty Ltd | Target detection using temperature controlled probes |
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| WO2025141105A1 (en) | 2023-12-27 | 2025-07-03 | bioMérieux | Specific probes for the detection of nucleic acids, methods and uses |
| EP4606908A1 (en) | 2024-02-21 | 2025-08-27 | Roche Diagnostics GmbH | Method of increasing the number of usable channels in multiplex pcr |
Family Cites Families (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4250257A (en) * | 1978-08-24 | 1981-02-10 | Technicon Instruments Corporation | Whole blood analyses in porous media |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US5405774A (en) | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
| US5352600A (en) | 1986-08-22 | 1994-10-04 | Hoffmann-La Roche Inc. | Purified thermostable enzyme |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| EP0894860B1 (en) | 1990-09-28 | 2012-12-12 | F. Hoffmann-La Roche AG | Thermostable DNA polymerases and deletion mutants thereof |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| DE4234086A1 (de) | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| US5541311A (en) | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
| US5843691A (en) * | 1993-05-15 | 1998-12-01 | Lifescan, Inc. | Visually-readable reagent test strip |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| US5571673A (en) | 1994-11-23 | 1996-11-05 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| WO1997009451A1 (en) | 1995-09-08 | 1997-03-13 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| CA2591550C (en) | 1996-06-04 | 2009-04-28 | University Of Utah Research Foundation | Monitoring hybridization during pcr |
| US6427156B1 (en) | 1997-01-21 | 2002-07-30 | Xilinx, Inc. | Configurable logic block with AND gate for efficient multiplication in FPGAS |
| JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
| US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
| US6472156B1 (en) | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
| US7585632B2 (en) * | 1999-10-29 | 2009-09-08 | Hologic, Inc. | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US7381532B2 (en) | 1999-10-29 | 2008-06-03 | Stratagene California | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US20050053939A1 (en) | 2001-11-09 | 2005-03-10 | Ahmed Chenna | Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents |
| KR101503072B1 (ko) | 2005-07-20 | 2015-03-16 | 바이엘 헬스케어 엘엘씨 | 게이트형 전류 측정법 |
| US7833716B2 (en) * | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| US8076067B2 (en) * | 2006-08-15 | 2011-12-13 | Genetag Technology, Inc. | Probe-antiprobe compositions and methods for DNA or RNA detection |
| EP2548647B1 (en) | 2006-10-20 | 2018-08-15 | CLONDIAG GmbH | Assay devices and methods for the detection of analytes |
| US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| US8133701B2 (en) | 2006-12-05 | 2012-03-13 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| WO2008083261A1 (en) | 2006-12-29 | 2008-07-10 | Applera Corporation | Systems and methods for detecting nucleic acids |
| GB0703997D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Methods for detecting nucleic sequences |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| GB0909333D0 (en) * | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| CA2738792C (en) | 2008-07-31 | 2016-11-22 | Oxitec Limited | Multiplex amplification and detection |
| US20120252015A1 (en) * | 2011-02-18 | 2012-10-04 | Bio-Rad Laboratories | Methods and compositions for detecting genetic material |
| KR101073875B1 (ko) * | 2008-11-28 | 2011-10-14 | 한국생명공학연구원 | 대장암 진단 마커 및 이를 이용한 대장암 진단방법 |
| US8385895B2 (en) | 2008-12-18 | 2013-02-26 | Verizon Patent And Licensing Inc. | Updating an operating parameter |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| KR101177320B1 (ko) * | 2010-02-26 | 2012-09-10 | 문우철 | Ｙ형 프로브 및 이것의 변형형, 및 이를 이용한 ｄｎａ 마이크로어레이, 키트 및 유전자 분석방법 |
| US8361720B2 (en) * | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| MX352460B (es) | 2011-01-11 | 2017-11-24 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
| KR20130101952A (ko) * | 2012-02-02 | 2013-09-16 | 주식회사 씨젠 | Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
| US10106842B2 (en) | 2013-03-15 | 2018-10-23 | Roche Molecular Systems, Inc. | Nucleic acid target identification by structure based probe cleavage |
| US9828629B2 (en) | 2013-03-15 | 2017-11-28 | Roche Molecular Systems, Inc. | Nucleic acid target identification by structure based probe cleavage |
| US20140378327A1 (en) | 2013-06-19 | 2014-12-25 | Luminex Corporation | Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres |
| US9637791B2 (en) | 2013-12-20 | 2017-05-02 | Roche Molecular Systems, Inc. | Multiplexed nucleic acid target identification by strucutre based probe cleavage |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| KR102465287B1 (ko) | 2014-08-11 | 2022-11-09 | 루미넥스 코포레이션 | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 |
| US10876155B2 (en) | 2014-12-09 | 2020-12-29 | Seegene, Inc. | Differentiation of signals for target nucleic acid sequences |
| KR102016747B1 (ko) | 2014-12-09 | 2019-09-02 | 주식회사 씨젠 | 상이한 검출 온도 및 기준값을 사용한 타겟 핵산 서열의 검출 |
| US20180355413A1 (en) * | 2014-12-22 | 2018-12-13 | Anapa Biotech A/S | Dual quenching assay for multiplex detection of target nucleic acids |
| US10590469B2 (en) * | 2016-09-15 | 2020-03-17 | Roche Molecular Svstems, Inc. | Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch |
| AU2017328617B2 (en) | 2016-09-15 | 2023-06-29 | F. Hoffmann-La Roche Ag | Methods for performing multiplexed real-time PCR |
-
2017
- 2017-09-15 AU AU2017328617A patent/AU2017328617B2/en active Active
- 2017-09-15 JP JP2019514212A patent/JP7063886B2/ja active Active
- 2017-09-15 CN CN201780056599.2A patent/CN109689889A/zh active Pending
- 2017-09-15 CA CA3036119A patent/CA3036119C/en active Active
- 2017-09-15 WO PCT/EP2017/073293 patent/WO2018050828A1/en not_active Ceased
- 2017-09-15 EP EP17768765.4A patent/EP3512959B1/en active Active
- 2017-09-15 KR KR1020197007697A patent/KR102468633B1/ko active Active
- 2017-09-15 US US15/705,772 patent/US11028433B2/en active Active
- 2017-09-15 WO PCT/EP2017/073288 patent/WO2018050824A1/en not_active Ceased
- 2017-09-15 ES ES17768765T patent/ES2893411T3/es active Active
- 2017-09-15 AU AU2017328613A patent/AU2017328613B2/en active Active
- 2017-09-15 JP JP2019514294A patent/JP7221199B2/ja active Active
- 2017-09-15 KR KR1020197007698A patent/KR102468174B1/ko active Active
- 2017-09-15 CN CN201780056666.0A patent/CN109689884A/zh active Pending
- 2017-09-15 EP EP17768768.8A patent/EP3512960B1/en active Active
- 2017-09-15 DK DK17768765.4T patent/DK3512959T3/da active
- 2017-09-15 CA CA3033250A patent/CA3033250C/en active Active
- 2017-09-15 US US15/705,821 patent/US11034997B2/en active Active
- 2017-09-15 ES ES17768768T patent/ES2933176T3/es active Active
-
2021
- 2021-05-07 US US17/314,470 patent/US11345958B2/en active Active
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