JP2019532628A5 - - Google Patents
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- JP2019532628A5 JP2019532628A5 JP2019514294A JP2019514294A JP2019532628A5 JP 2019532628 A5 JP2019532628 A5 JP 2019532628A5 JP 2019514294 A JP2019514294 A JP 2019514294A JP 2019514294 A JP2019514294 A JP 2019514294A JP 2019532628 A5 JP2019532628 A5 JP 2019532628A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- oligonucleotide
- quenching
- component
- tag portion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662395325P | 2016-09-15 | 2016-09-15 | |
| US62/395,325 | 2016-09-15 | ||
| US201662435595P | 2016-12-16 | 2016-12-16 | |
| US62/435,595 | 2016-12-16 | ||
| US201762536871P | 2017-07-25 | 2017-07-25 | |
| US62/536,871 | 2017-07-25 | ||
| PCT/EP2017/073288 WO2018050824A1 (en) | 2016-09-15 | 2017-09-15 | Methods for performing multiplexed pcr |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019532628A JP2019532628A (ja) | 2019-11-14 |
| JP2019532628A5 true JP2019532628A5 (enExample) | 2020-10-22 |
| JP7221199B2 JP7221199B2 (ja) | 2023-02-20 |
Family
ID=59901522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019514212A Active JP7063886B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスリアルタイムｐｃｒを実施する方法 |
| JP2019514294A Active JP7221199B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスｐｃｒの実施方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019514212A Active JP7063886B2 (ja) | 2016-09-15 | 2017-09-15 | マルチプレックスリアルタイムｐｃｒを実施する方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US11034997B2 (enExample) |
| EP (2) | EP3512960B1 (enExample) |
| JP (2) | JP7063886B2 (enExample) |
| KR (2) | KR102468174B1 (enExample) |
| CN (2) | CN109689889A (enExample) |
| AU (2) | AU2017328613B2 (enExample) |
| CA (2) | CA3036119C (enExample) |
| DK (1) | DK3512959T3 (enExample) |
| ES (2) | ES2933176T3 (enExample) |
| WO (2) | WO2018050828A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102468174B1 (ko) | 2016-09-15 | 2022-11-17 | 에프. 호프만-라 로슈 아게 | 멀티플렉스 pcr 수행 방법 |
| RU2665631C2 (ru) * | 2017-01-25 | 2018-09-03 | Общество с ограниченной ответственностью "Секвойя Дженетикс" | Способ специфической идентификации последовательностей днк |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| JP2021511056A (ja) | 2018-01-22 | 2021-05-06 | ルミネックス コーポレーション | 不連続融解分析のための方法および組成物 |
| US20190292596A1 (en) | 2018-03-21 | 2019-09-26 | Roche Molecular Systems, Inc. | Modified nucleoside phosphates with high thermal stability |
| BR112020019755A2 (pt) | 2018-04-20 | 2021-01-26 | Seegene, Inc. | método e aparelho para detectar uma pluralidade de sequências de ácidos nucleicos alvo na amostra |
| US11384376B2 (en) | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| KR102796204B1 (ko) * | 2018-08-09 | 2025-04-14 | 스피디엑스 피티와이 리미티드 | 핵산의 다중 검출 |
| US11371080B2 (en) | 2018-11-30 | 2022-06-28 | Luminex Corporation | Methods and probes for performing PCR with melt analysis for increased multiplexing |
| CN113195734B (zh) * | 2018-12-20 | 2024-12-24 | 豪夫迈·罗氏有限公司 | 通过固相分子成像检测靶核酸 |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| JP2023505327A (ja) | 2019-12-09 | 2023-02-08 | エフ．ホフマン－ラ ロシュ アーゲー | ジカチオン性蛍光色素 |
| CN111534518B (zh) * | 2020-05-18 | 2021-07-23 | 纳昂达(南京)生物科技有限公司 | 通用封闭序列及其应用 |
| EP4172355A4 (en) * | 2020-06-30 | 2024-09-25 | SpeeDx Pty Ltd | MULTIPLEX DETECTION OF NUCLEIC ACIDS USING MIXED REPORTERS |
| CN112195221B (zh) * | 2020-09-13 | 2021-08-06 | 武汉纳诺德医疗科技有限公司 | 一种多温度单荧光通道多重实时荧光定量pcr仪及其检测方法 |
| WO2022060967A1 (en) * | 2020-09-16 | 2022-03-24 | Nanostring Technologies, Inc. | Chemical compositions and methods of using the same |
| US20240035077A1 (en) | 2020-12-22 | 2024-02-01 | Roche Molecular Systems, Inc. | Methods for performing multiplexed real-time pcr with the use of large stokes shift fluorescent dyes |
| JP2024522640A (ja) | 2021-06-25 | 2024-06-21 | エフ． ホフマン－ラ ロシュ アーゲー | 高感度で温度多重化ｐｃｒを行うための方法 |
| WO2023056345A1 (en) * | 2021-09-30 | 2023-04-06 | Gen-Probe Incorporated | Temperature-selectable fret cassette signaling |
| CN119487130A (zh) | 2022-06-28 | 2025-02-18 | 豪夫迈·罗氏有限公司 | 具有大斯托克斯位移的荧光染料 |
| EP4621068A1 (en) * | 2022-11-16 | 2025-09-24 | Seegene, Inc. | Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay |
| AR132405A1 (es) * | 2023-04-19 | 2025-06-25 | Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet | Métodos y sistemas para seleccionar biomarcadores basados en metilación del adn para la detección de cáncer colorrectal |
| WO2024234030A1 (en) * | 2023-05-15 | 2024-11-21 | Speedx Pty Ltd | Target detection using temperature controlled probes |
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| WO2025141105A1 (en) | 2023-12-27 | 2025-07-03 | bioMérieux | Specific probes for the detection of nucleic acids, methods and uses |
| EP4606908A1 (en) * | 2024-02-21 | 2025-08-27 | Roche Diagnostics GmbH | Method of increasing the number of usable channels in multiplex pcr |
Family Cites Families (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4250257A (en) * | 1978-08-24 | 1981-02-10 | Technicon Instruments Corporation | Whole blood analyses in porous media |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5405774A (en) | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
| US5352600A (en) | 1986-08-22 | 1994-10-04 | Hoffmann-La Roche Inc. | Purified thermostable enzyme |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| AU663474B2 (en) | 1990-09-28 | 1995-10-12 | F. Hoffmann-La Roche Ag | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| DE4234086A1 (de) | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| US5541311A (en) | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
| US5843691A (en) * | 1993-05-15 | 1998-12-01 | Lifescan, Inc. | Visually-readable reagent test strip |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| US5571673A (en) | 1994-11-23 | 1996-11-05 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| WO1997009451A1 (en) | 1995-09-08 | 1997-03-13 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| WO1997046714A1 (en) | 1996-06-04 | 1997-12-11 | University Of Utah Research Foundation | Monitoring hybridization during pcr |
| US6427156B1 (en) | 1997-01-21 | 2002-07-30 | Xilinx, Inc. | Configurable logic block with AND gate for efficient multiplication in FPGAS |
| JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
| US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
| US6472156B1 (en) | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
| US7381532B2 (en) | 1999-10-29 | 2008-06-03 | Stratagene California | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US7585632B2 (en) * | 1999-10-29 | 2009-09-08 | Hologic, Inc. | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US20050053939A1 (en) | 2001-11-09 | 2005-03-10 | Ahmed Chenna | Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents |
| ES2717135T3 (es) | 2005-07-20 | 2019-06-19 | Ascensia Diabetes Care Holdings Ag | Método para señalar al usuario para que añada una muestra adicional a una tira de prueba, método para medir la temperatura de una muestra y métodos para determinar la concentración de un analito basados en amperometría controlada |
| US7833716B2 (en) * | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| EP2059614A4 (en) * | 2006-08-15 | 2010-06-30 | Zygene Biotech Llc | ANTISONDON PROBE COMPOSITIONS AND METHODS OF DETECTION OF DNA OR RNA |
| EP2548647B1 (en) | 2006-10-20 | 2018-08-15 | CLONDIAG GmbH | Assay devices and methods for the detection of analytes |
| US8133701B2 (en) | 2006-12-05 | 2012-03-13 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| US20080241838A1 (en) | 2006-12-29 | 2008-10-02 | Applera Corporation, Applied Biosystems Group | Methods and systems for detecting nucleic acids |
| GB0703997D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Methods for detecting nucleic sequences |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| GB0909333D0 (en) * | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| CN102171366B (zh) * | 2008-07-31 | 2014-11-26 | 奥西泰克有限公司 | 多重扩增与检测 |
| US20120252015A1 (en) * | 2011-02-18 | 2012-10-04 | Bio-Rad Laboratories | Methods and compositions for detecting genetic material |
| KR101073875B1 (ko) * | 2008-11-28 | 2011-10-14 | 한국생명공학연구원 | 대장암 진단 마커 및 이를 이용한 대장암 진단방법 |
| US8385895B2 (en) | 2008-12-18 | 2013-02-26 | Verizon Patent And Licensing Inc. | Updating an operating parameter |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| KR101177320B1 (ko) * | 2010-02-26 | 2012-09-10 | 문우철 | Ｙ형 프로브 및 이것의 변형형, 및 이를 이용한 ｄｎａ 마이크로어레이, 키트 및 유전자 분석방법 |
| US8361720B2 (en) * | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| MX352460B (es) | 2011-01-11 | 2017-11-24 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
| KR20130101952A (ko) * | 2012-02-02 | 2013-09-16 | 주식회사 씨젠 | Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
| US10106842B2 (en) | 2013-03-15 | 2018-10-23 | Roche Molecular Systems, Inc. | Nucleic acid target identification by structure based probe cleavage |
| US9828629B2 (en) | 2013-03-15 | 2017-11-28 | Roche Molecular Systems, Inc. | Nucleic acid target identification by structure based probe cleavage |
| US20140378327A1 (en) | 2013-06-19 | 2014-12-25 | Luminex Corporation | Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres |
| US9637791B2 (en) | 2013-12-20 | 2017-05-02 | Roche Molecular Systems, Inc. | Multiplexed nucleic acid target identification by strucutre based probe cleavage |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| KR20220155394A (ko) | 2014-08-11 | 2022-11-22 | 루미넥스 코포레이션 | 핵산 검정에서 개선된 용융 판별 및 멀티플렉싱을 위한 프로브 |
| US10876155B2 (en) | 2014-12-09 | 2020-12-29 | Seegene, Inc. | Differentiation of signals for target nucleic acid sequences |
| JP2018504096A (ja) | 2014-12-09 | 2018-02-15 | シージーン アイエヌシー | 異なる検出温度及び基準値を使用したターゲット核酸配列の検出 |
| JP2018500932A (ja) * | 2014-12-22 | 2018-01-18 | アナーパ バイオテック エー／エス | 標的核酸のマルチプレックス検出のためのデュアルクエンチングアッセイ |
| US10590469B2 (en) * | 2016-09-15 | 2020-03-17 | Roche Molecular Svstems, Inc. | Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch |
| KR102468174B1 (ko) | 2016-09-15 | 2022-11-17 | 에프. 호프만-라 로슈 아게 | 멀티플렉스 pcr 수행 방법 |
-
2017
- 2017-09-15 KR KR1020197007698A patent/KR102468174B1/ko active Active
- 2017-09-15 CN CN201780056599.2A patent/CN109689889A/zh active Pending
- 2017-09-15 ES ES17768768T patent/ES2933176T3/es active Active
- 2017-09-15 WO PCT/EP2017/073293 patent/WO2018050828A1/en not_active Ceased
- 2017-09-15 EP EP17768768.8A patent/EP3512960B1/en active Active
- 2017-09-15 CA CA3036119A patent/CA3036119C/en active Active
- 2017-09-15 AU AU2017328613A patent/AU2017328613B2/en active Active
- 2017-09-15 US US15/705,821 patent/US11034997B2/en active Active
- 2017-09-15 AU AU2017328617A patent/AU2017328617B2/en active Active
- 2017-09-15 JP JP2019514212A patent/JP7063886B2/ja active Active
- 2017-09-15 DK DK17768765.4T patent/DK3512959T3/da active
- 2017-09-15 ES ES17768765T patent/ES2893411T3/es active Active
- 2017-09-15 JP JP2019514294A patent/JP7221199B2/ja active Active
- 2017-09-15 CA CA3033250A patent/CA3033250C/en active Active
- 2017-09-15 EP EP17768765.4A patent/EP3512959B1/en active Active
- 2017-09-15 KR KR1020197007697A patent/KR102468633B1/ko active Active
- 2017-09-15 WO PCT/EP2017/073288 patent/WO2018050824A1/en not_active Ceased
- 2017-09-15 CN CN201780056666.0A patent/CN109689884A/zh active Pending
- 2017-09-15 US US15/705,772 patent/US11028433B2/en active Active
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2021
- 2021-05-07 US US17/314,470 patent/US11345958B2/en active Active
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