JP2002502615A5 - - Google Patents
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- JP2002502615A5 JP2002502615A5 JP2000530635A JP2000530635A JP2002502615A5 JP 2002502615 A5 JP2002502615 A5 JP 2002502615A5 JP 2000530635 A JP2000530635 A JP 2000530635A JP 2000530635 A JP2000530635 A JP 2000530635A JP 2002502615 A5 JP2002502615 A5 JP 2002502615A5
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- allele
- nucleic acid
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- 239000000523 sample Substances 0.000 description 120
- 238000000034 method Methods 0.000 description 92
- 108700028369 Alleles Proteins 0.000 description 53
- 230000003321 amplification Effects 0.000 description 51
- 238000003199 nucleic acid amplification method Methods 0.000 description 51
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 49
- 239000002751 oligonucleotide probe Substances 0.000 description 49
- 108020004414 DNA Proteins 0.000 description 44
- 238000002844 melting Methods 0.000 description 40
- 230000008018 melting Effects 0.000 description 40
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 39
- 108020004707 nucleic acids Proteins 0.000 description 39
- 102000039446 nucleic acids Human genes 0.000 description 39
- 150000007523 nucleic acids Chemical class 0.000 description 39
- 238000002189 fluorescence spectrum Methods 0.000 description 27
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 20
- 239000011541 reaction mixture Substances 0.000 description 19
- 239000003795 chemical substances by application Substances 0.000 description 15
- 108091093088 Amplicon Proteins 0.000 description 14
- 108010010803 Gelatin Proteins 0.000 description 13
- 238000000137 annealing Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 229920000159 gelatin Polymers 0.000 description 13
- 239000008273 gelatin Substances 0.000 description 13
- 235000019322 gelatine Nutrition 0.000 description 13
- 235000011852 gelatine desserts Nutrition 0.000 description 13
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 13
- 229920001213 Polysorbate 20 Polymers 0.000 description 12
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 11
- 238000010791 quenching Methods 0.000 description 11
- 101710163270 Nuclease Proteins 0.000 description 10
- 108010037497 3'-nucleotidase Proteins 0.000 description 9
- 239000007983 Tris buffer Substances 0.000 description 7
- 239000000975 dye Substances 0.000 description 7
- 238000001228 spectrum Methods 0.000 description 7
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 4
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 4
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 4
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 101150037123 APOE gene Proteins 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 101100216294 Danio rerio apoeb gene Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1859598A | 1998-02-04 | 1998-02-04 | |
| US09/018,595 | 1998-02-04 | ||
| PCT/US1999/000499 WO1999040226A2 (en) | 1998-02-04 | 1999-01-08 | Determination of a genotype of an amplification product at multiple allelic sites |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002502615A JP2002502615A (ja) | 2002-01-29 |
| JP2002502615A5 true JP2002502615A5 (enExample) | 2006-02-16 |
| JP4388694B2 JP4388694B2 (ja) | 2009-12-24 |
Family
ID=21788757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000530635A Expired - Lifetime JP4388694B2 (ja) | 1998-02-04 | 1999-01-08 | 複数のアレル部位における増幅産物のゲノム型決定 |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US6154707A (enExample) |
| EP (1) | EP1053348B1 (enExample) |
| JP (1) | JP4388694B2 (enExample) |
| AT (1) | ATE469982T1 (enExample) |
| AU (1) | AU758463B2 (enExample) |
| CA (1) | CA2318880C (enExample) |
| DE (1) | DE69942444D1 (enExample) |
| WO (1) | WO1999040226A2 (enExample) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2288760T3 (es) | 1996-04-25 | 2008-01-16 | Bioarray Solutions Ltd. | Ensamblaje electrocinetico controlado por luz de particulas proximas a superficies. |
| AU1232099A (en) * | 1997-11-04 | 1999-05-24 | Roche Diagnostics Gmbh | Specific and sensitive method for detecting nucleic acids |
| DE69942444D1 (de) * | 1998-02-04 | 2010-07-15 | Life Technologies Corp | Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen |
| DE60030145T2 (de) | 1999-10-22 | 2008-01-10 | PHRI Properties, Inc., Newark | Nachweisverfahren für kurze sequenzvarianten |
| US7585632B2 (en) * | 1999-10-29 | 2009-09-08 | Hologic, Inc. | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US6660845B1 (en) | 1999-11-23 | 2003-12-09 | Epoch Biosciences, Inc. | Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof |
| US20040081959A9 (en) | 1999-12-08 | 2004-04-29 | Epoch Biosciences, Inc. | Fluorescent quenching detection reagents and methods |
| US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
| JP2001299392A (ja) * | 2000-04-25 | 2001-10-30 | Otsuka Pharmaceut Co Ltd | Ｃａｔｃｈ２２症候群検出方法 |
| JP2004511210A (ja) * | 2000-05-23 | 2004-04-15 | バリアジェニックス インコーポレーテッド | 配列変動を検出する、ｄｎａを遺伝分析するための方法 |
| US7435541B2 (en) | 2000-05-23 | 2008-10-14 | Sequenom, Inc. | Restriction enzyme genotyping |
| AU2001272993B2 (en) | 2000-06-21 | 2005-03-10 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis |
| US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
| GB0016516D0 (en) * | 2000-07-06 | 2000-08-23 | Owen Mumford Ltd | Improvements relating to needle protection devices |
| US7267945B2 (en) * | 2001-03-26 | 2007-09-11 | Applera Corporation | Methods of determining the presence of polynucleotides employing amplification |
| US20030143554A1 (en) * | 2001-03-31 | 2003-07-31 | Berres Mark E. | Method of genotyping by determination of allele copy number |
| US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
| AU2002318450B2 (en) * | 2001-06-28 | 2007-11-22 | Grifols Worldwide Operations Limited | Diagnostic assays for parvovirus B19 |
| KR20040068122A (ko) | 2001-10-15 | 2004-07-30 | 바이오어레이 솔루션스 리미티드 | 공동 검색과 효소-매개된 탐지에 의한 다형성 좌위의 다중분석 |
| EP1439222A1 (en) * | 2001-10-26 | 2004-07-21 | Matsushita Electric Industrial Co., Ltd. | Method of detecting target nucleic acid and nucleic acid probe |
| US20050079493A1 (en) * | 2002-11-09 | 2005-04-14 | Tam Joseph Wing On | DNA fingerprinting using allelic specific oligonucleotide reversed dot blot (ASO-RDB) flow through hybridization process and device |
| US7732138B2 (en) * | 2001-11-07 | 2010-06-08 | Diagcor Bioscience Incorporation Limited | Rapid genotyping analysis and the device thereof |
| US20110111389A1 (en) | 2001-11-07 | 2011-05-12 | Diagcor Bioscience Incorporation Limited | Rapid genotyping analysis for human papillomavirus and the device thereof |
| AU2003216489A1 (en) * | 2002-03-05 | 2003-09-22 | M.C.W Research Foundation, Inc. | Methods and compositions for pharmacological and toxicological evaluation of test agents |
| US7176002B2 (en) * | 2002-05-16 | 2007-02-13 | Applera Corporation | Universal-tagged oligonucleotide primers and methods of use |
| JP4457001B2 (ja) * | 2002-05-31 | 2010-04-28 | セクレタリー・デパートメント・オブ・アトミック・エナジー | ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法 |
| AU2003901871A0 (en) * | 2003-03-31 | 2003-05-08 | Vision Biosystems Limited | A method and apparatus for fluid dispensation, preparation and dilation |
| CN105258988A (zh) | 2002-06-20 | 2016-01-20 | 徕卡病理系统墨尔本控股有限公司 | 带有排放机构的生物反应装置 |
| CA2494993A1 (en) * | 2002-08-21 | 2004-03-04 | Epoch Biosciences, Inc. | Abasic site endonuclease assay |
| AU2003298655A1 (en) | 2002-11-15 | 2004-06-15 | Bioarray Solutions, Ltd. | Analysis, secure access to, and transmission of array images |
| AU2003296994A1 (en) * | 2002-12-13 | 2004-07-09 | Applera Corporation | Methods for identifying, viewing, and analyzing syntenic and orthologous genomic regions between two or more species |
| US7851150B2 (en) | 2002-12-18 | 2010-12-14 | Third Wave Technologies, Inc. | Detection of small nucleic acids |
| CA2512110A1 (en) | 2002-12-31 | 2004-07-22 | Mmi Genomics, Inc. | Compositions, methods, and systems for inferring bovine breed |
| US7858301B2 (en) * | 2003-05-07 | 2010-12-28 | Bioarray Solutions, Ltd. | Method of probe design for nucleic acid analysis by multiplexed hybridization |
| ES2385828T3 (es) | 2003-06-20 | 2012-08-01 | Exiqon A/S | Sondas, bibliotecas y kits para análisis de mezclas de ácidos nucleicos y procedimientos para construirlos |
| US20050038776A1 (en) * | 2003-08-15 | 2005-02-17 | Ramin Cyrus | Information system for biological and life sciences research |
| WO2005029705A2 (en) | 2003-09-18 | 2005-03-31 | Bioarray Solutions, Ltd. | Number coding for identification of subtypes of coded types of solid phase carriers |
| US20050221357A1 (en) * | 2003-09-19 | 2005-10-06 | Mark Shannon | Normalization of gene expression data |
| US7417726B2 (en) * | 2003-09-19 | 2008-08-26 | Applied Biosystems Inc. | Normalization of data using controls |
| US7595279B2 (en) | 2003-09-22 | 2009-09-29 | Bioarray Solutions Ltd. | Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules |
| US7332280B2 (en) * | 2003-10-14 | 2008-02-19 | Ronald Levy | Classification of patients having diffuse large B-cell lymphoma based upon gene expression |
| EP1692298A4 (en) | 2003-10-28 | 2008-08-13 | Bioarray Solutions Ltd | OPTIMIZATION OF GENE EXPRESSION ANALYSIS USING IMMOBILIZED CATCHES |
| NZ547495A (en) | 2003-10-29 | 2008-05-30 | Bioarray Solutions Ltd | Multiplexed nucleic acid analysis by fragmentation of double-stranded DNA |
| US7439341B2 (en) | 2003-11-14 | 2008-10-21 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
| EP1561823A1 (de) * | 2004-02-04 | 2005-08-10 | Biotez Berlin-Buch GmbH | Verfahren zum Nachweis von Einzelnukleotid-polymorphismen (SNP) in Genen des Arzneimittelmetabolismus und Testkit zur Durchführung des Verfahrens |
| US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
| US7575863B2 (en) | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
| US7642055B2 (en) * | 2004-09-21 | 2010-01-05 | Applied Biosystems, Llc | Two-color real-time/end-point quantitation of microRNAs (miRNAs) |
| US20060111915A1 (en) * | 2004-11-23 | 2006-05-25 | Applera Corporation | Hypothesis generation |
| US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
| US20080213762A1 (en) * | 2004-12-08 | 2008-09-04 | Takeshi Yamamoto | Method of Gene Sequence Examination |
| US20060166238A1 (en) * | 2004-12-22 | 2006-07-27 | Ramsing Niels B | Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same |
| WO2006127507A2 (en) * | 2005-05-20 | 2006-11-30 | Integrated Dna Technologies, Inc. | Compounds and methods for labeling oligonucleotides |
| US8486629B2 (en) | 2005-06-01 | 2013-07-16 | Bioarray Solutions, Ltd. | Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation |
| EP2477029A1 (en) | 2005-06-02 | 2012-07-18 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| EP1928571B1 (en) * | 2005-08-22 | 2013-03-13 | Life Technologies Corporation | Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible |
| US8372584B2 (en) | 2006-06-14 | 2013-02-12 | The General Hospital Corporation | Rare cell analysis using sample splitting and DNA tags |
| WO2007147018A1 (en) * | 2006-06-14 | 2007-12-21 | Cellpoint Diagnostics, Inc. | Analysis of rare cell-enriched samples |
| EP3424598B1 (en) * | 2006-06-14 | 2022-06-08 | Verinata Health, Inc. | Rare cell analysis using sample splitting and dna tags |
| US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| EP2029779A4 (en) | 2006-06-14 | 2010-01-20 | Living Microsystems Inc | HIGHLY PARALLEL SNP GENOTYPING UTILIZATION FOR FETAL DIAGNOSIS |
| ATE437965T1 (de) * | 2006-10-12 | 2009-08-15 | Bio Rad Pasteur | Doppelsträngige sonden zur fluoreszenzdetektion von nukleinsäuren |
| DE102007013099A1 (de) | 2007-03-14 | 2008-09-18 | Aj Innuscreen Gmbh | Verfahren und Testkit zum schnellen Nachweis spezifischer Nukleinsäuresequenzen, insbesondere zum Nachweis von Mutationen oder SNP's |
| EP2116614A1 (en) * | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| US8383346B2 (en) * | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| EP2285958B1 (en) * | 2008-06-13 | 2016-03-09 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| SMT201700149T1 (it) | 2008-09-20 | 2017-05-08 | Univ Leland Stanford Junior | Diagnosi non invasiva di aneuploidia fetale mediante sequenziamento |
| US8412466B2 (en) * | 2009-04-13 | 2013-04-02 | Canon U.S. Life Sciences, Inc. | Rapid method of pattern recognition, machine learning, and automated genotype classification through correlation analysis of dynamic signals |
| US20110312503A1 (en) | 2010-01-23 | 2011-12-22 | Artemis Health, Inc. | Methods of fetal abnormality detection |
| AU2011230496B2 (en) | 2010-03-26 | 2015-09-17 | Integrated Dna Technologies, Inc. | Methods for enhancing nucleic acid hybridization |
| US9506057B2 (en) | 2010-03-26 | 2016-11-29 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| CA2809457C (en) | 2010-09-07 | 2019-07-30 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| US20120083035A1 (en) | 2010-09-30 | 2012-04-05 | Dharmacon, Inc. | Modified Cell Lines for Increasing Lentiviral Titers |
| US9617588B2 (en) | 2010-10-05 | 2017-04-11 | Thermo Fisher Scientific Baltics Uab | Enzyme mixture |
| JP2015519900A (ja) | 2012-05-21 | 2015-07-16 | フリューダイム・コーポレイション | 粒子集団の単粒子解析方法及び単粒子単離方法 |
| EP4671381A3 (en) * | 2016-04-06 | 2026-01-28 | Life Technologies Corporation | Methods for synthesis and detection of nucleic acids |
| JP6846636B2 (ja) * | 2017-02-28 | 2021-03-24 | パナソニックＩｐマネジメント株式会社 | 核酸検出用オリゴヌクレオチド |
| WO2022155397A1 (en) * | 2021-01-15 | 2022-07-21 | Nuprobe Usa, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5075216A (en) | 1988-09-23 | 1991-12-24 | Cetus Corporation | Methods for dna sequencing with thermus aquaticus dna polymerase |
| DK0540693T3 (da) | 1990-07-24 | 1999-09-13 | Hoffmann La Roche | Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5403707A (en) * | 1993-05-14 | 1995-04-04 | Eastman Kodak Company | Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5834181A (en) | 1994-07-28 | 1998-11-10 | Genzyme Corporation | High throughput screening method for sequences or genetic alterations in nucleic acids |
| EP0803058B1 (en) * | 1994-12-30 | 2008-11-05 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| DE19524579C2 (de) * | 1995-07-06 | 1997-12-18 | Daimler Benz Ag | Transformator als Strombegrenzer |
| US5792610A (en) * | 1996-05-01 | 1998-08-11 | Biorad Laboratories, Inc. | Method for conducting multiparametric fluorescence in situ hybridization |
| US5759781A (en) * | 1995-12-22 | 1998-06-02 | Yale University | Multiparametric fluorescence in situ hybridization |
| AU713667B2 (en) * | 1996-04-12 | 1999-12-09 | Phri Properties, Inc. | Detection probes, kits and assays |
| US6015667A (en) * | 1996-06-03 | 2000-01-18 | The Perkin-Emer Corporation | Multicomponent analysis method including the determination of a statistical confidence interval |
| US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
| DE69942444D1 (de) * | 1998-02-04 | 2010-07-15 | Life Technologies Corp | Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen |
| US5952202A (en) * | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
-
1999
- 1999-01-08 DE DE69942444T patent/DE69942444D1/de not_active Expired - Lifetime
- 1999-01-08 AT AT99903026T patent/ATE469982T1/de not_active IP Right Cessation
- 1999-01-08 EP EP99903026A patent/EP1053348B1/en not_active Expired - Lifetime
- 1999-01-08 AU AU23144/99A patent/AU758463B2/en not_active Ceased
- 1999-01-08 CA CA2318880A patent/CA2318880C/en not_active Expired - Fee Related
- 1999-01-08 WO PCT/US1999/000499 patent/WO1999040226A2/en not_active Ceased
- 1999-01-08 JP JP2000530635A patent/JP4388694B2/ja not_active Expired - Lifetime
- 1999-06-03 US US09/324,709 patent/US6154707A/en not_active Expired - Lifetime
-
2002
- 2002-03-21 US US10/104,774 patent/US6884583B2/en not_active Expired - Lifetime
-
2003
- 2003-06-04 US US10/455,150 patent/US7132239B2/en not_active Expired - Fee Related
-
2006
- 2006-11-03 US US11/592,716 patent/US20080187913A1/en not_active Abandoned
-
2009
- 2009-11-13 US US12/618,686 patent/US20120053069A1/en not_active Abandoned
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