JP2002502615A5 - - Google Patents
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- JP2002502615A5 JP2002502615A5 JP2000530635A JP2000530635A JP2002502615A5 JP 2002502615 A5 JP2002502615 A5 JP 2002502615A5 JP 2000530635 A JP2000530635 A JP 2000530635A JP 2000530635 A JP2000530635 A JP 2000530635A JP 2002502615 A5 JP2002502615 A5 JP 2002502615A5
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- probes
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- fluorescence
- allele
- nucleic acid
- Prior art date
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- 238000000034 method Methods 0.000 description 92
- 108700028369 Alleles Proteins 0.000 description 53
- 230000003321 amplification Effects 0.000 description 51
- 238000003199 nucleic acid amplification method Methods 0.000 description 51
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 49
- 239000002751 oligonucleotide probe Substances 0.000 description 49
- 108020004414 DNA Proteins 0.000 description 44
- 238000002844 melting Methods 0.000 description 40
- 230000008018 melting Effects 0.000 description 40
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 39
- 108020004707 nucleic acids Proteins 0.000 description 39
- 102000039446 nucleic acids Human genes 0.000 description 39
- 150000007523 nucleic acids Chemical class 0.000 description 39
- 238000002189 fluorescence spectrum Methods 0.000 description 27
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 20
- 239000011541 reaction mixture Substances 0.000 description 19
- 239000003795 chemical substances by application Substances 0.000 description 15
- 108091093088 Amplicon Proteins 0.000 description 14
- 108010010803 Gelatin Proteins 0.000 description 13
- 238000000137 annealing Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
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- 235000019322 gelatine Nutrition 0.000 description 13
- 235000011852 gelatine desserts Nutrition 0.000 description 13
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 13
- 229920001213 Polysorbate 20 Polymers 0.000 description 12
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 11
- 238000010791 quenching Methods 0.000 description 11
- 101710163270 Nuclease Proteins 0.000 description 10
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- 239000007983 Tris buffer Substances 0.000 description 7
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- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
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- AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 4
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 4
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 4
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
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- 102000053602 DNA Human genes 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
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- 101150037123 APOE gene Proteins 0.000 description 1
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- 101100216294 Danio rerio apoeb gene Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1859598A | 1998-02-04 | 1998-02-04 | |
| US09/018,595 | 1998-02-04 | ||
| PCT/US1999/000499 WO1999040226A2 (en) | 1998-02-04 | 1999-01-08 | Determination of a genotype of an amplification product at multiple allelic sites |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002502615A JP2002502615A (ja) | 2002-01-29 |
| JP2002502615A5 true JP2002502615A5 (enExample) | 2006-02-16 |
| JP4388694B2 JP4388694B2 (ja) | 2009-12-24 |
Family
ID=21788757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000530635A Expired - Lifetime JP4388694B2 (ja) | 1998-02-04 | 1999-01-08 | 複数のアレル部位における増幅産物のゲノム型決定 |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US6154707A (enExample) |
| EP (1) | EP1053348B1 (enExample) |
| JP (1) | JP4388694B2 (enExample) |
| AT (1) | ATE469982T1 (enExample) |
| AU (1) | AU758463B2 (enExample) |
| CA (1) | CA2318880C (enExample) |
| DE (1) | DE69942444D1 (enExample) |
| WO (1) | WO1999040226A2 (enExample) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6660845B1 (en) | 1999-11-23 | 2003-12-09 | Epoch Biosciences, Inc. | Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof |
| US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
| US20040081959A9 (en) | 1999-12-08 | 2004-04-29 | Epoch Biosciences, Inc. | Fluorescent quenching detection reagents and methods |
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| WO1997039008A1 (en) * | 1996-04-12 | 1997-10-23 | The Public Health Research Institute Of The City Of New York, Inc. | Detection probes, kits and assays |
| US6015667A (en) * | 1996-06-03 | 2000-01-18 | The Perkin-Emer Corporation | Multicomponent analysis method including the determination of a statistical confidence interval |
| US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
| CA2318880C (en) | 1998-02-04 | 2010-07-27 | Perkin-Elmer Corporation | Determination of a genotype of an amplification product at multiple allelic sites |
| US5952202A (en) * | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
-
1999
- 1999-01-08 CA CA2318880A patent/CA2318880C/en not_active Expired - Fee Related
- 1999-01-08 JP JP2000530635A patent/JP4388694B2/ja not_active Expired - Lifetime
- 1999-01-08 AT AT99903026T patent/ATE469982T1/de not_active IP Right Cessation
- 1999-01-08 DE DE69942444T patent/DE69942444D1/de not_active Expired - Lifetime
- 1999-01-08 EP EP99903026A patent/EP1053348B1/en not_active Expired - Lifetime
- 1999-01-08 AU AU23144/99A patent/AU758463B2/en not_active Ceased
- 1999-01-08 WO PCT/US1999/000499 patent/WO1999040226A2/en not_active Ceased
- 1999-06-03 US US09/324,709 patent/US6154707A/en not_active Expired - Lifetime
-
2002
- 2002-03-21 US US10/104,774 patent/US6884583B2/en not_active Expired - Lifetime
-
2003
- 2003-06-04 US US10/455,150 patent/US7132239B2/en not_active Expired - Fee Related
-
2006
- 2006-11-03 US US11/592,716 patent/US20080187913A1/en not_active Abandoned
-
2009
- 2009-11-13 US US12/618,686 patent/US20120053069A1/en not_active Abandoned
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