JP2011507522A - 豚サーコウイルスに対してブタを免疫化するための方法および組成物 - Google Patents
豚サーコウイルスに対してブタを免疫化するための方法および組成物 Download PDFInfo
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- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/10011—Circoviridae
- C12N2750/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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| JP2016191501A Pending JP2017060473A (ja) | 2007-12-21 | 2016-09-29 | 豚サーコウイルスに対してブタを免疫化するための方法および組成物 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| AR078253A1 (es) | 2009-09-02 | 2011-10-26 | Boehringer Ingelheim Vetmed | Metodos para reducir la actividad antivirica en composiciones pcv-2 y composiciones pcv-2 con mejor inmunogenicidad |
| CN102711816A (zh) * | 2009-10-22 | 2012-10-03 | 莱比锡大学 | 患有牛新生儿全血细胞减少症的小牛中的圆环病毒的检测 |
| EP2528931A4 (en) * | 2010-01-25 | 2013-12-04 | Blood Systems Inc | CYCLOVIRUS AND USE METHOD THEREFOR |
| HRP20200258T1 (hr) | 2010-03-16 | 2020-09-04 | Virginia Tech Intellectual Properties Inc | Živo atenuirano himerno cjepivo protiv svinjskog cirkovirusa |
| CA2804604C (en) * | 2010-07-08 | 2023-10-03 | United Biomedical, Inc. | Designer peptide-based pcv2 vaccine |
| CN101920012B (zh) * | 2010-07-22 | 2012-12-12 | 普莱柯生物工程股份有限公司 | 利用家蚕生物反应器生产猪圆环病毒2型重组衣壳蛋白亚单位疫苗的方法及其产品 |
| KR101030792B1 (ko) * | 2010-09-16 | 2011-04-27 | 주식회사 코미팜 | 돼지 써코바이러스２(ｐｃｖ２) 유전자의 표면발현용 벡터 및 이로 형질전환된 살모넬라 백신 균주 |
| CN102199571B (zh) * | 2011-04-06 | 2013-07-31 | 华中农业大学 | 一种表达猪2型圆环病毒orf2基因片段的重组支气管败血波氏杆菌株、疫苗及应用 |
| CN102988978B (zh) * | 2011-08-01 | 2016-02-03 | 普莱柯生物工程股份有限公司 | 含有猪圆环病毒2型抗原与副猪嗜血杆菌抗原的疫苗组合物及其制备方法与应用 |
| HUP1100470A2 (en) | 2011-08-30 | 2013-03-28 | Mezoegazdasagi Biotechnologiai Kutatokoezpont | Nanoparticle-based veterinary vaccine |
| EP2564869A1 (en) | 2011-09-02 | 2013-03-06 | Ceva Sante Animale | Synthetic capsid proteins and uses thereof |
| CN103083655B (zh) * | 2011-11-02 | 2015-08-26 | 普莱柯生物工程股份有限公司 | 预防和治疗猪圆环病毒2型、副猪嗜血杆菌和猪肺炎支原体感染的疫苗组合物及其制备方法 |
| CN102517331A (zh) * | 2011-12-26 | 2012-06-27 | 武汉中博生物股份有限公司 | 一种猪圆环病毒2型亚单位疫苗及其制备方法和其应用 |
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| EP2789346A1 (en) | 2013-04-11 | 2014-10-15 | CEVA Santé Animale SA | Fusion polypeptides and vaccines |
| CN103275938B (zh) * | 2013-05-07 | 2015-02-25 | 上海市农业科学院 | 猪圆环病毒弱毒疫苗株的制备方法 |
| DK2994162T3 (en) | 2013-05-08 | 2021-06-21 | Pharmgate Biologics Inc | Vaccine for pcv2 og mycoplasma |
| EP3035958B1 (en) | 2013-08-23 | 2022-10-26 | Boehringer Ingelheim Animal Health USA Inc. | Porcine circovirus type 2 (pcv2) subunit vaccine |
| RU2662685C2 (ru) * | 2013-09-25 | 2018-07-26 | Зоэтис Сервисиз Ллс | Pcv2b дивергентная вакцинная композиция и способы её применения |
| KR20220083861A (ko) | 2013-10-02 | 2022-06-20 | 베링거 인겔하임 애니멀 헬스 유에스에이 인크. | Ｐｃｖ2 ｏｒｆ2 단백질 변이체 및 이로 이루어진 바이러스 유사 입자 |
| CN104248759B (zh) * | 2013-11-19 | 2017-05-10 | 普莱柯生物工程股份有限公司 | 一种疫苗组合物及其制备方法和应用 |
| CN104250640A (zh) | 2014-08-22 | 2014-12-31 | 普莱柯生物工程股份有限公司 | 猪伪狂犬病病毒基因缺失株、疫苗组合物及其制备方法和应用 |
| CN105709220B (zh) * | 2014-12-03 | 2020-03-31 | 普莱柯生物工程股份有限公司 | 用于猪圆环病毒与猪流感的疫苗组合物及制备方法和应用 |
| EP3034609A1 (en) | 2014-12-19 | 2016-06-22 | Ceva Sante Animale | Recombinant swinepox virus and vaccines |
| CN104984335A (zh) * | 2015-07-14 | 2015-10-21 | 浙江诺倍威生物技术有限公司 | 猪圆环病毒双亚型orf2共表达载体构建及疫苗制备 |
| JP7088835B2 (ja) * | 2015-10-16 | 2022-06-21 | カンザス ステイト ユニバーシティ リサーチ ファウンデーション | ブタサーコウイルス３型免疫原性組成物、その製造方法、およびその使用方法 |
| MY191905A (en) * | 2015-12-28 | 2022-07-18 | Boehringer Ingelheim Animal Health Usa Inc | M hyo multivalent vaccine and uses thereof |
| CN105785037B (zh) * | 2016-03-30 | 2017-09-19 | 中国农业科学院兰州兽医研究所 | 猪圆环病毒2型抗体快速检测层析试纸条及制备方法 |
| EP3254692A1 (en) | 2016-06-10 | 2017-12-13 | Ceva Sante Animale | Multivalent recombinant spv |
| CN106435016B (zh) * | 2016-08-30 | 2019-07-26 | 中国农业科学院兰州兽医研究所 | 一种快速显色一步法检测猪圆环病毒2型的lamp试剂盒 |
| CN107937354A (zh) * | 2017-11-10 | 2018-04-20 | 南京天邦生物科技有限公司 | 2型猪圆环病毒及其应用 |
| US20200390878A1 (en) | 2017-12-08 | 2020-12-17 | Ceva Sante Animale | Recombinant swinepox virus and vaccines |
| KR102133632B1 (ko) * | 2018-03-29 | 2020-07-13 | 충남대학교산학협력단 | 재조합 돼지 써코바이러스 및 이를 포함하는 돼지 써코바이러스 감염 질환의 예방용 백신 조성물 |
| JP2021527073A (ja) | 2018-06-11 | 2021-10-11 | セヴァ サンテ アニマレＣｅｖａ Ｓａｎｔｅ Ａｎｉｍａｌｅ | ブタサーコウイルスに対するワクチン接種 |
| CN109381696A (zh) * | 2018-08-27 | 2019-02-26 | 长沙创西生物科技有限公司 | 一种用于防治猪圆环病毒病的缓释微囊的制备方法 |
| CN111088396A (zh) * | 2019-11-14 | 2020-05-01 | 河南科技学院 | 一种同时检测副猪嗜血杆菌、猪细小病毒、猪圆环病毒2型的三重实时荧光pcr方法 |
| RU2747468C1 (ru) * | 2020-06-23 | 2021-05-05 | Федеральное Казенное Предприятие "Щелковский Биокомбинат" | Способ получения вакцины против цирковируса свиней (варианты) |
| CN113907044A (zh) * | 2021-11-09 | 2022-01-11 | 福建省连江县刘氏兔业养殖场 | 一种抗病猪品系的培育方法及其应用 |
| CN115820840A (zh) * | 2022-11-23 | 2023-03-21 | 武汉轻工大学 | 一种副猪嗜血杆菌感染后引起仔猪免疫抑制的分子标记物 |
| US20250332242A1 (en) | 2024-04-30 | 2025-10-30 | Zoetis Services Llc | Porcine endogenous retrovirus (perv)-free porcine circovirus type 2d-based vaccines and methods of use |
| US20250332243A1 (en) | 2024-04-30 | 2025-10-30 | Zoetis Services Llc | Methods of manufacturing porcine endogenous retrovirus (perv) free animal health vaccines |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050238662A1 (en) * | 1997-12-05 | 2005-10-27 | Agence Francaise De Securite Sanitaire Des Aliments | Circovirus sequences associated with piglet weight loss disease (PWD) |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4708871A (en) * | 1983-03-08 | 1987-11-24 | Commonwealth Serum Laboratories Commission | Antigenically active amino acid sequences |
| US4567043A (en) * | 1983-06-15 | 1986-01-28 | American Home Products Corporation (Del.) | Canine corona virus vaccine |
| US5238662A (en) * | 1987-07-31 | 1993-08-24 | Chevron Research Company | Processes for recovering precious metals |
| US5147966A (en) * | 1990-07-31 | 1992-09-15 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Polyimide molding powder, coating, adhesive and matrix resin |
| FR2781159B1 (fr) * | 1998-07-06 | 2000-10-06 | Merial Sas | Vaccin circovirus et parvovirus porcin |
| US6391314B1 (en) * | 1997-10-03 | 2002-05-21 | Merial | Porcine circoviruses vaccines diagnostic reagents |
| UA78180C2 (uk) * | 1997-10-03 | 2007-03-15 | Меріаль | Кільцевий вірус свині типу ii, вакцини та діагностичні реагенти |
| FR2769322B1 (fr) * | 1997-10-03 | 2002-03-08 | Merial Sas | Nouveaux circovirus porcins, vaccins et reactifs de diagnostic |
| FR2772047B1 (fr) * | 1997-12-05 | 2004-04-09 | Ct Nat D Etudes Veterinaires E | Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection |
| WO1999045956A1 (en) * | 1998-03-13 | 1999-09-16 | University Of Georgia Research Foundation, Inc. | Vaccines against circovirus infections |
| CA2442135C (en) * | 2001-03-27 | 2008-08-19 | University Of Saskatchewan | Methods to culture circovirus |
| US20030096377A1 (en) * | 2001-06-28 | 2003-05-22 | Virginia Tech Intellectual Properties, Inc. | Differential PCR-RFLP assay for detecting and distinguishing between nonpathogenic PCV-1 and pathogenic PCV-2 |
| US7279166B2 (en) * | 2001-12-12 | 2007-10-09 | Virginia Tech Intellectual Properties, Inc. | Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof |
| US7276353B2 (en) * | 2001-12-12 | 2007-10-02 | Virginia Tech Intellectual Properties, Inc. | Chimeric infectious DNA clones, chimeric porcine circoviruses and uses thereof |
| DK2371383T3 (en) * | 2005-12-29 | 2015-11-30 | Boehringer Ingelheim Vetmed | Use of a PCV2 immunogenic composition for reduction of clinical symptoms in pigs |
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2008
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050238662A1 (en) * | 1997-12-05 | 2005-10-27 | Agence Francaise De Securite Sanitaire Des Aliments | Circovirus sequences associated with piglet weight loss disease (PWD) |
Non-Patent Citations (2)
| Title |
|---|
| JPN6013026678; Ａｒｃｈ． Ｖｉｒｏｌ． Ｖｏｌ． １５２, 200705, ｐ． １０３５-１０４４ * |
| JPN6013026680; Ｄａｔａｂａｓｅ ＧｅｎＢａｎｋ， Ａｃｃｅｓｓｉｏｎ Ｎｏ． ＤＱ６２９１１５ , 20070614 * |
Also Published As
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| RU2493254C2 (ru) | 2013-09-20 |
| ME01156B (me) | 2013-03-20 |
| CA2710247A1 (en) | 2009-07-09 |
| CN101932700A (zh) | 2010-12-29 |
| CA2710247C (en) | 2014-02-18 |
| AU2008343172A1 (en) | 2009-07-09 |
| RU2010124791A (ru) | 2012-01-27 |
| BRPI0821286A8 (pt) | 2017-08-15 |
| JP2017060473A (ja) | 2017-03-30 |
| WO2009085912A1 (en) | 2009-07-09 |
| CL2008003813A1 (es) | 2009-03-20 |
| US20090162398A1 (en) | 2009-06-25 |
| EP2225367A1 (en) | 2010-09-08 |
| BRPI0821286A2 (pt) | 2014-10-14 |
| AR069882A1 (es) | 2010-02-24 |
| CO6290791A2 (es) | 2011-06-20 |
| KR20100094587A (ko) | 2010-08-26 |
| US20110305725A1 (en) | 2011-12-15 |
| AU2008343172B2 (en) | 2015-02-19 |
| NZ586238A (en) | 2012-10-26 |
| RU2493254C9 (ru) | 2013-12-20 |
| UA99495C2 (ru) | 2012-08-27 |
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