JP2009509565A5 - - Google Patents

Download PDF

Info

Publication number
JP2009509565A5
JP2009509565A5 JP2008534568A JP2008534568A JP2009509565A5 JP 2009509565 A5 JP2009509565 A5 JP 2009509565A5 JP 2008534568 A JP2008534568 A JP 2008534568A JP 2008534568 A JP2008534568 A JP 2008534568A JP 2009509565 A5 JP2009509565 A5 JP 2009509565A5
Authority
JP
Japan
Prior art keywords
region
quencher
inhibitor
nucleotide sequence
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2008534568A
Other languages
English (en)
Japanese (ja)
Other versions
JP2009509565A (ja
JP5438320B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2006/037829 external-priority patent/WO2007041201A2/en
Publication of JP2009509565A publication Critical patent/JP2009509565A/ja
Publication of JP2009509565A5 publication Critical patent/JP2009509565A5/ja
Application granted granted Critical
Publication of JP5438320B2 publication Critical patent/JP5438320B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2008534568A 2005-10-03 2006-09-29 核酸を増幅するための組成物、方法およびキット Active JP5438320B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US72338305P 2005-10-03 2005-10-03
US60/723,383 2005-10-03
PCT/US2006/037829 WO2007041201A2 (en) 2005-10-03 2006-09-29 Compositions, methods, and kits for amplifying nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2012181035A Division JP5890762B2 (ja) 2005-10-03 2012-08-17 核酸を増幅するための組成物、方法およびキット

Publications (3)

Publication Number Publication Date
JP2009509565A JP2009509565A (ja) 2009-03-12
JP2009509565A5 true JP2009509565A5 (https=) 2012-10-04
JP5438320B2 JP5438320B2 (ja) 2014-03-12

Family

ID=37906699

Family Applications (9)

Application Number Title Priority Date Filing Date
JP2008534568A Active JP5438320B2 (ja) 2005-10-03 2006-09-29 核酸を増幅するための組成物、方法およびキット
JP2012181035A Active JP5890762B2 (ja) 2005-10-03 2012-08-17 核酸を増幅するための組成物、方法およびキット
JP2015127341A Active JP6157541B2 (ja) 2005-10-03 2015-06-25 核酸を増幅するための組成物、方法およびキット
JP2016162389A Withdrawn JP2016192979A (ja) 2005-10-03 2016-08-23 核酸を増幅するための組成物、方法およびキット
JP2018083860A Active JP7274264B2 (ja) 2005-10-03 2018-04-25 核酸を増幅するための組成物、方法およびキット
JP2019131946A Withdrawn JP2019193664A (ja) 2005-10-03 2019-07-17 核酸を増幅するための組成物、方法およびキット
JP2021137897A Pending JP2021182939A (ja) 2005-10-03 2021-08-26 核酸を増幅するための組成物、方法およびキット
JP2023031224A Pending JP2023065578A (ja) 2005-10-03 2023-03-01 核酸を増幅するための組成物、方法およびキット
JP2023135399A Pending JP2023156506A (ja) 2005-10-03 2023-08-23 核酸を増幅するための組成物、方法およびキット

Family Applications After (8)

Application Number Title Priority Date Filing Date
JP2012181035A Active JP5890762B2 (ja) 2005-10-03 2012-08-17 核酸を増幅するための組成物、方法およびキット
JP2015127341A Active JP6157541B2 (ja) 2005-10-03 2015-06-25 核酸を増幅するための組成物、方法およびキット
JP2016162389A Withdrawn JP2016192979A (ja) 2005-10-03 2016-08-23 核酸を増幅するための組成物、方法およびキット
JP2018083860A Active JP7274264B2 (ja) 2005-10-03 2018-04-25 核酸を増幅するための組成物、方法およびキット
JP2019131946A Withdrawn JP2019193664A (ja) 2005-10-03 2019-07-17 核酸を増幅するための組成物、方法およびキット
JP2021137897A Pending JP2021182939A (ja) 2005-10-03 2021-08-26 核酸を増幅するための組成物、方法およびキット
JP2023031224A Pending JP2023065578A (ja) 2005-10-03 2023-03-01 核酸を増幅するための組成物、方法およびキット
JP2023135399A Pending JP2023156506A (ja) 2005-10-03 2023-08-23 核酸を増幅するための組成物、方法およびキット

Country Status (6)

Country Link
US (6) US20070212704A1 (https=)
EP (2) EP2458011B1 (https=)
JP (9) JP5438320B2 (https=)
CN (2) CN101321877B (https=)
CA (1) CA2624634A1 (https=)
WO (1) WO2007041201A2 (https=)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
JP4542312B2 (ja) 2001-03-09 2010-09-15 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
EP1802774B1 (en) * 2004-10-18 2012-01-11 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP2458011B1 (en) 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US20090203531A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
GB2470672B (en) * 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
DK2396459T3 (en) 2009-02-13 2017-08-28 X-Chem Inc Method for preparing and screening DNA-encoded libraries
US20120165202A1 (en) * 2009-04-30 2012-06-28 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) * 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
JP5977000B2 (ja) * 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
US9109226B2 (en) 2011-09-01 2015-08-18 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
DK2748357T3 (en) 2011-09-07 2018-07-16 X Chem Inc Methods for labeling DNA-encoded libraries
WO2013038534A1 (ja) * 2011-09-14 2013-03-21 日本碍子株式会社 標的核酸の検出方法
MY157586A (en) * 2011-09-14 2016-06-16 Ngk Insulators Ltd Method for detecting target nucleic acid
CN103998921B (zh) * 2011-10-14 2016-04-20 贝克顿·迪金森公司 方波热循环
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EA201992285A1 (ru) * 2012-07-13 2020-05-31 Икс-Чем, Инк. Днк-кодированные библиотеки, содержащие связи кодирующих олигонуклеотидов, не доступные для считывания полимеразами
WO2014018367A1 (en) * 2012-07-23 2014-01-30 Cynvenio Biosystems, Inc. Dual enzymatic amplification
WO2014028793A1 (en) * 2012-08-17 2014-02-20 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Cyclopentane-peptide nucleic acids for qualitative and quantitative detection of nucleic acids
EP2912197B1 (en) 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
AU2013334635B2 (en) 2012-10-24 2018-12-06 Becton, Dickinson And Company Hydroxamate substituted azaindoline-cyanine dyes and bioconjugates of the same
JP2015050980A (ja) * 2013-09-09 2015-03-19 東洋紡株式会社 非特異増幅を低減させる方法
EP4389888A3 (en) 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
WO2015094861A1 (en) 2013-12-17 2015-06-25 Clontech Laboratories, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
JP2017531424A (ja) * 2014-09-17 2017-10-26 セラノス， インコーポレイテッドＴｈｅｒａｎｏｓ， Ｉｎｃ． ハイブリッド多元ステップ核酸増幅
CN107208146B (zh) * 2014-11-20 2022-01-18 安普里怀斯公司 用于核酸扩增的组合物和方法
US10240178B2 (en) 2014-12-19 2019-03-26 Brandeis University Mispriming prevention reagents
EP4095261B1 (en) 2015-01-06 2025-05-28 Molecular Loop Biosciences, Inc. Screening for structural variants
AU2016250576C1 (en) * 2015-04-23 2021-05-06 Geron Corporation Methods of polynucleotide preparation using multivalent cation salt compositions
AU2016252998B2 (en) * 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
CN107980065B (zh) * 2015-05-11 2021-09-07 3M创新有限公司 用于减少核酸扩增抑制的组合物
PT3436198T (pt) * 2016-03-29 2022-07-13 Univ Rice William M Deteção de ácido nucleico baseada em superfícies num dispositivo fluídico de fluxo de convecção
EP3464596B1 (en) * 2016-06-07 2025-07-30 Cepheid Thermostable polymerase inhibitor compositions and methods
WO2018031588A1 (en) * 2016-08-09 2018-02-15 Takara Bio Usa, Inc. Nucleic acid adaptors with molecular identification sequences and use thereof
CN118207299A (zh) 2016-08-26 2024-06-18 生命技术公司 核酸提取和扩增对照及其使用方法
US20190284621A1 (en) * 2016-11-11 2019-09-19 Roche Innovation Center Copenhagen A/S Therapeutic oligonucleotides capture and detection
US20180188204A1 (en) * 2017-01-01 2018-07-05 Sylvester Tumusiime Use of charged quinine sulfate or other precursors or derivatives of quinine alkaloids in visualization of nucleic acids
CN108546748A (zh) * 2017-04-05 2018-09-18 杭州丹威生物科技有限公司 一种检测核酸的方法和试剂盒
EA202091673A1 (ru) * 2018-01-29 2021-02-03 Ст. Джуд Чилдрен'С Рисерч Хоспитал, Инк. Способ амплификации нуклеиновых кислот
CN112941155B (zh) * 2018-05-14 2023-07-14 北京艾克伦医疗科技有限公司 具有茎环结构的dna引物对及其应用
WO2020018824A1 (en) 2018-07-19 2020-01-23 Ultima Genomics, Inc. Nucleic acid clonal amplification and sequencing methods, systems, and kits
MX2021007311A (es) * 2018-12-20 2021-09-21 Alveo Tech Inc Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.
CN110628877B (zh) * 2019-05-17 2024-07-16 澳门大学 增强核酸扩增反应的增强剂、试剂盒和进行核酸扩增反应的方法
CN113528624B (zh) * 2020-04-17 2025-12-02 青岛简码基因科技有限公司 扩增和检测核酸的方法及试剂盒
JP2023531027A (ja) * 2020-06-22 2023-07-20 ▲蘇▼州新海生物科技股▲ふん▼有限公司 核酸リガンド、およびその使用
JP2023538231A (ja) * 2020-07-23 2023-09-07 エンゾー バイオケム，インコーポレイテッド 蛍光クエンチャーポリマーコンジュゲート及びその使用
CN111926067B (zh) * 2020-09-24 2021-01-08 圣湘生物科技股份有限公司 用于荧光定量pcr的双探针组合物、试剂盒、用途及方法
KR102661292B1 (ko) * 2021-09-09 2024-04-30 포항공과대학교 산학협력단 금속 이온 반응성 dna 중합 효소 스위치 및 이에 의해 제어되는 등온 증폭 방법
JP2025531820A (ja) * 2022-09-07 2025-09-25 ベクトン・ディキンソン・アンド・カンパニー 検出特異性改善のための修飾分子ビーコン
EP4627112A1 (en) * 2022-12-01 2025-10-08 Becton, Dickinson and Company Asymmetric hairpin probes for nucleic acid detection

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US6004826A (en) 1988-07-20 1999-12-21 David Segev Repair-mediated process for amplifying and detecting nucleic acid sequences
US5763173A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5874557A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5693502A (en) * 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
WO1992001814A2 (en) 1990-07-24 1992-02-06 F. Hoffmann-La-Roche Ag The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5614402A (en) 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
WO1995030139A1 (en) 1994-04-29 1995-11-09 Perkin-Elmer Corporation System for real time detection of nucleic acid amplification products
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
DE69638318D1 (de) 1995-06-07 2011-02-17 Gilead Sciences Inc Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren
US6183967B1 (en) 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
ES2192672T3 (es) 1996-11-18 2003-10-16 Takeshi Imanishi Nuevos analogos de nucleotidos.
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6143877A (en) 1997-04-30 2000-11-07 Epoch Pharmaceuticals, Inc. Oligonucleotides including pyrazolo[3,4-D]pyrimidine bases, bound in double stranded nucleic acids
AU9063398A (en) 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6127121A (en) 1998-04-03 2000-10-03 Epoch Pharmaceuticals, Inc. Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
EP1095054B1 (en) 1998-07-09 2006-10-25 Biocept, Inc. Method of using an improved peptide nucleic acid universal library to optimize dna sequence hybridation
AU782555B2 (en) * 1999-07-02 2005-08-11 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6830902B1 (en) 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
MXPA02002656A (es) 1999-09-13 2003-10-14 Nugen Technologies Inc Metodos y composiciones para la ampliacion lineal isotermica de secuencias de polinucleotidos.
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
JP3463098B2 (ja) * 1999-10-08 2003-11-05 独立行政法人産業技術総合研究所 モジュレートアプタマー及びこれを用いた標的タンパク質の検出方法
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US6579680B2 (en) * 2000-02-28 2003-06-17 Corning Incorporated Method for label-free detection of hybridized DNA targets
DE60141205D1 (de) * 2000-04-03 2010-03-18 Cytyc Corp Nachweis und typisierung des papillomavirus mittels pna-sonden
SE0001768D0 (sv) 2000-05-12 2000-05-12 Helen Andersson Mikrofluidisk flödescell för manipulering av partiklar
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
JP2004507230A (ja) 2000-07-03 2004-03-11 アプレラ　コーポレイション ポリヌクレオチド配列アッセイ
WO2002006827A1 (en) * 2000-07-05 2002-01-24 The General Hospital Corporation Compounds and methods for fluorescently labeling nucleic acids
US7309573B2 (en) * 2000-11-21 2007-12-18 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7267945B2 (en) 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
AU2003213836A1 (en) 2002-03-11 2003-09-29 Epoch Biosciences, Inc. Negatively charged minor groove binders
EP1527175B1 (en) * 2002-06-24 2009-05-27 Exiqon A/S Methods and systems for detection and isolation of a nucleotide sequence
WO2004011666A2 (en) 2002-07-26 2004-02-05 Applera Corporation Mg-mediated hot start biochemical reactions
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
CN1661359B (zh) * 2004-02-25 2013-06-12 陕西西大北美基因股份有限公司 一种核酸等温扩增定量检测方法
EP1802774B1 (en) * 2004-10-18 2012-01-11 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
CN1308461C (zh) * 2004-12-24 2007-04-04 武汉大学 一种检测口蹄疫病毒的荧光定量pcr试剂盒及应用
WO2007038422A2 (en) * 2005-09-26 2007-04-05 Allelogic Biosciences Corp. Oligonucleotides as temperature-sensitive inhibitors for dna polymerases
US7917762B2 (en) 2005-09-30 2011-03-29 Phoenix Technologies Ltd. Secure execution environment by preventing execution of unauthorized boot loaders
EP2458011B1 (en) * 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids

Similar Documents

Publication Publication Date Title
JP2009509565A5 (https=)
ES2550237T3 (es) Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
CN104583413B (zh) 用于自rna模板开始的等温dna扩增试剂盒
Nordström et al. Method enabling pyrosequencing on double-stranded DNA
ES2450073T3 (es) Amplificación de ácidos nucleicos en presencia de oligómeros aleatorios modificados
Ohtsuki et al. The minimal tRNA: unique structure of Ascaris suum mitochondrial tRNASerUCU having a short T arm and lacking the entire D arm
JP5022383B2 (ja) マグネシウム封鎖によるｐｃｒホットスタート
US10457982B2 (en) Method for nucleic acid amplification
JP2016537003A (ja) バックグラウンド減少のための分解可能なアダプター
KR20120058520A (ko) 역전사 및 증폭반응에서 핵산 오염을 제거하는 방법
ES2964592T3 (es) Circularización y amplificación de ácido nucleico asistida por ligasa
US10100292B2 (en) Mutant endonuclease V enzymes and applications thereof
CN104603268A (zh) 协同引物、探针及其应用
US20120219945A1 (en) Use of single-stranded binding protein in amplifying target nucleic acid
KR20070073917A (ko) 개선된 증폭을 위한 핵산 복구
CN101705273A (zh) 通过包括热循环的多重置换扩增来扩增靶核酸序列的方法
KR102014989B1 (ko) 폴리뉴클레오티드 및 그의 용도
JP6880034B2 (ja) 特異的ｒｎａの安定化のための汎用法
JP5436105B2 (ja) 改善された核酸増幅のためのポリアニオン
US20140004508A1 (en) Method for isothermal dna amplification starting from an rna template
CN111868259B (zh) 具有高热稳定性的经修饰核苷磷酸
KR20130014996A (ko) 분해가능한 키메라 프로브를 사용한 웨스트 나일 바이러스의 실시간 검출 방법
KR101466666B1 (ko) 열 안정성 dna 중합효소의 제조방법 및 이에 의해 제조된 dna 중합효소
JP2011512821A (ja) コールドショックタンパク質組成物、並びにその使用のための方法及びキット
Kranaster et al. A mutated thermostable Thermus aquaticus DNA polymerase with reverse transcriptase activity for one step RNA pathogen detection