MX2021007311A - Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica. - Google Patents

Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.

Info

Publication number
MX2021007311A
MX2021007311A MX2021007311A MX2021007311A MX2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A MX 2021007311 A MX2021007311 A MX 2021007311A
Authority
MX
Mexico
Prior art keywords
amplification
compositions
methods
reactions
reduce nonspecific
Prior art date
Application number
MX2021007311A
Other languages
English (en)
Inventor
Joseph Carl Gaiteri
Rixun Fang
Brenna Hearn Lord
Yuh- Min CHIANG
Ronald Phillip Chiarello
Original Assignee
Alveo Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alveo Tech Inc filed Critical Alveo Tech Inc
Publication of MX2021007311A publication Critical patent/MX2021007311A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Las modalidades se refieren a métodos, sistemas y composiciones para reducir la amplificación no específica en reacciones de amplificación isotérmica. Algunas modalidades se refieren a la reducción de la amplificación no específica en reacciones de amplificación isotérmica mediada por bucle (LAMP) con ciertos oligonucleótidos.
MX2021007311A 2018-12-20 2019-12-18 Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica. MX2021007311A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862782610P 2018-12-20 2018-12-20
PCT/US2019/067134 WO2020132042A1 (en) 2018-12-20 2019-12-18 Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions

Publications (1)

Publication Number Publication Date
MX2021007311A true MX2021007311A (es) 2021-09-21

Family

ID=71101681

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021007311A MX2021007311A (es) 2018-12-20 2019-12-18 Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.

Country Status (8)

Country Link
US (1) US20220056511A1 (es)
EP (1) EP3899023A4 (es)
JP (1) JP2022515192A (es)
CN (1) CN113454236A (es)
AU (1) AU2019403220A1 (es)
CA (1) CA3123839A1 (es)
MX (1) MX2021007311A (es)
WO (1) WO2020132042A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
AU2017330304A1 (en) 2016-09-23 2019-04-11 Alveo Technologies, Inc. Methods and compositions for detecting analytes
JP2023536962A (ja) * 2020-08-06 2023-08-30 エフ． ホフマン－ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－２）、インフルエンザａ及びインフルエンザｂの検出のための組成物及び方法
WO2024054867A1 (en) * 2022-09-07 2024-03-14 Becton, Dickinson And Company Modified molecular beacons for improved detection specificity

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070090158A (ko) * 2004-10-18 2007-09-05 브랜데이스 유니버시티 Ｐｃｒ 증폭에서 재현성을 개선시키고 미스프라이밍을감소시키기 위한 시약 및 방법
JP5438320B2 (ja) * 2005-10-03 2014-03-12 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 核酸を増幅するための組成物、方法およびキット
WO2007060949A1 (ja) * 2005-11-25 2007-05-31 K.K. Dnaform 核酸検出及び増幅方法
JP5025489B2 (ja) * 2005-12-28 2012-09-12 学校法人麻布獣医学園 標的配列の特異的高感度増幅法
WO2012075231A1 (en) * 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
WO2012121225A1 (ja) * 2011-03-04 2012-09-13 株式会社カネカ 核酸の検出方法及びその方法に用いるデバイス、キット
EP3058100A4 (en) * 2013-10-18 2017-04-19 California Institute of Technology Enhanced nucleic acid identification and detection
JP2017537648A (ja) * 2014-12-19 2017-12-21 ブランデイズ ユニバーシティー ミスプライミング防止試薬

Also Published As

Publication number Publication date
EP3899023A4 (en) 2022-10-12
WO2020132042A1 (en) 2020-06-25
CA3123839A1 (en) 2020-06-25
CN113454236A (zh) 2021-09-28
AU2019403220A1 (en) 2021-07-15
EP3899023A1 (en) 2021-10-27
JP2022515192A (ja) 2022-02-17
US20220056511A1 (en) 2022-02-24

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