JP2007512027A - トランスジェニック動物の産生における干渉ｒｎａの使用 - Google Patents

トランスジェニック動物の産生における干渉ｒｎａの使用 Download PDF

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JP2007512027A
JP2007512027A JP2006541617A JP2006541617A JP2007512027A JP 2007512027 A JP2007512027 A JP 2007512027A JP 2006541617 A JP2006541617 A JP 2006541617A JP 2006541617 A JP2006541617 A JP 2006541617A JP 2007512027 A JP2007512027 A JP 2007512027A
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irna
gene
cells
cell
sequence
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JP2007512027A5 (enExample
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ケビン ウェルズ，
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レビビコア， インコーポレイテッド
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2227/10—Mammal
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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JP2006541617A 2003-11-21 2004-11-22 トランスジェニック動物の産生における干渉ｒｎａの使用 Pending JP2007512027A (ja)

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US52393803P 2003-11-21 2003-11-21
PCT/US2004/039191 WO2005081714A2 (en) 2003-11-21 2004-11-22 Use of interfering rna in the production of transgenic animals

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JP2007512027A true JP2007512027A (ja) 2007-05-17
JP2007512027A5 JP2007512027A5 (enExample) 2008-01-10

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US (1) US10793873B2 (enExample)
EP (2) EP2514826A3 (enExample)
JP (1) JP2007512027A (enExample)
CN (2) CN103397019A (enExample)
AU (1) AU2004316293A1 (enExample)
CA (1) CA2546853C (enExample)
WO (1) WO2005081714A2 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007525192A (ja) * 2003-05-23 2007-09-06 サーナ・セラピューティクス・インコーポレイテッド 化学修飾した低分子干渉核酸（ｓｉＮＡ）を使用する遺伝子発現のＲＮＡ干渉仲介抑制
JP2010528667A (ja) * 2007-06-13 2010-08-26 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション トリの生産形質の改変
JP2013501942A (ja) * 2009-08-14 2013-01-17 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 自閉症を診断及び治療する方法
US11098082B2 (en) 2015-06-26 2021-08-24 The Regents Of The University Of California Antigenic peptides and uses thereof for diagnosing and treating autism
JP2023551513A (ja) * 2020-12-01 2023-12-08 エー・テー・ハー・チューリッヒ 代替プロモータを含むｄｎａ構築物

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004259019B2 (en) * 2003-07-21 2010-09-23 Lifecell Corporation Acellular tissue matrices made from galactose alpha-1,3-galactose-deficient tissue
EP1582591A3 (en) * 2004-02-27 2005-12-21 Bundesrepublik Deutschland vertreten durch das Bundesminsterium für Gesundheit, dieses vertr. durch das Robert-Koch-Institut Sirna and their use for knock down expression of porcine endogenous retrovirus
ES2459640T3 (es) * 2005-06-24 2014-05-12 Recombinetics, Inc. Uso de citosina desaminasas para disminuir la transferencia de retroelementos desde cerdos a seres humanos
JP2007104969A (ja) * 2005-10-13 2007-04-26 Bio Think Tank Co Ltd ショートヘアピン型ＲＮＡ（ｓｈＲＮＡ）前駆体を産生するための核酸及びその利用
WO2008110624A2 (en) 2007-03-14 2008-09-18 Universita' Degli Studi Di Milano-Bicocca Sirna-mediated silencing of genes for treating chemotherapeutic drug-resistant epithelial tumors
ES2549122T3 (es) 2007-05-31 2015-10-23 University Of Iowa Research Foundation Reducción de toxicidad por interferencia de ARN desviada de su diana
WO2010008562A2 (en) * 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
EP2425015B1 (en) * 2009-04-29 2018-01-10 Ralph Wirtz A method to assess prognosis and to predict therapeutic success in cancer by determining hormone receptor expression levels
US9420770B2 (en) 2009-12-01 2016-08-23 Indiana University Research & Technology Corporation Methods of modulating thrombocytopenia and modified transgenic pigs
JP2013534824A (ja) * 2010-06-24 2013-09-09 ジ・オハイオ・ステート・ユニバーシティ 標的化されたｍｉＲ−２９発現によりマウスにおいてモデル化された慢性リンパ性白血病
EP2673286B1 (en) 2011-02-12 2019-07-03 University of Iowa Research Foundation Therapeutic compounds
EP2698379A4 (en) * 2011-03-18 2015-04-29 Shanghai Inst Biol Sciences INSECTROSIVE PREPARATION AND METHODS BASED ON RNAI TECHNOLOGY
WO2013188358A1 (en) * 2012-06-12 2013-12-19 The General Hospital Corporation Miniature swine transgenic for one or more coagulation factors
BR112016009178B1 (pt) 2013-11-04 2020-12-15 Lifecell Corporation Método de preparação de um produto de tecido e uso de um produto de tecido
CN104677866A (zh) * 2014-03-25 2015-06-03 中国科学院大连化学物理研究所 测定CP装载siRNA体系中siRNA释放的方法
RU2711147C2 (ru) 2014-05-20 2020-01-15 Юниверсити Оф Айова Рисерч Фаундейшн Терапевтические соединения для лечения болезни хантингтона
CN104232670A (zh) * 2014-08-25 2014-12-24 中国水产科学研究院黑龙江水产研究所 一种采用鱼类转基因方法构建的基于鲤鱼mstn干涉载体及其构建方法
NZ741778A (en) * 2015-10-08 2022-02-25 Harvard College Multiplexed genome editing
CN106918697B (zh) * 2017-02-15 2018-07-31 中国医学科学院北京协和医院 一种预测ra药物疗效的诊断标志物及其应用
CN111031790A (zh) * 2017-04-20 2020-04-17 E开创生物技术股份有限公司 产生基因修改的动物的方法
CN107760682B (zh) * 2017-10-18 2020-12-18 云南大学 一种可用于分子防治害虫的核酸序列及其应用
CN110628807B (zh) * 2018-05-30 2021-06-18 中国科学院植物研究所 盐角草SePSS蛋白及其编码基因与应用
US20210277421A1 (en) * 2018-08-06 2021-09-09 NemaMetrix, Inc Homologous Recombination Reporter Construct and Uses Thereof
JP7431837B2 (ja) 2018-10-05 2024-02-15 ゼノセラピューティクス インコーポレイテッド 異種移植製品及び異種移植方法
US10883084B2 (en) 2018-10-05 2021-01-05 Xenotherapeutics, Inc. Personalized cells, tissues, and organs for transplantation from a humanized, bespoke, designated-pathogen free, (non-human) donor and methods and products relating to same
AU2019377855A1 (en) 2018-11-08 2021-06-03 Greenlight Biosciences, Inc. Control of insect infestation
CN110055253A (zh) * 2019-04-28 2019-07-26 复旦大学附属金山医院 一种针对人半胱氨酸蛋白酶抑制剂B的siRNA分子及其应用
US20220012673A1 (en) * 2020-07-13 2022-01-13 ZenDesk, Inc. Maintaining status information for customer-support agents across multiple channels
US20240240178A1 (en) * 2021-05-06 2024-07-18 Systems Oncology, Llc Sirna constructs for inhibiting gene expression in targeted cancer cells
EP4347035A4 (en) * 2021-06-01 2025-09-03 Univ Massachusetts CAS9 NICKING-MEDIATED GENE EDITING
WO2023004365A1 (en) * 2021-07-20 2023-01-26 Sio Gene Therapies Inc. Vector constructs for delivery of nucleic acids encoding therapeutic proteasome activator complex subunits and methods of using the same
CN115011601B (zh) * 2022-06-27 2023-07-21 山东大学齐鲁医院 一种干扰JUND表达的shRNA、重组腺相关病毒载体及其应用
CN118581151B (zh) * 2024-05-29 2024-12-06 中国农业科学院兰州兽医研究所(中国动物卫生与流行病学中心兰州分中心) 一种猪becn1基因敲除细胞的构建方法及其应用
CN120608104B (zh) * 2025-08-12 2025-11-25 中国科学院水生生物研究所 lasp1基因在鱼类抗病毒免疫调控中的功能和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001068836A2 (en) * 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
WO2003006477A1 (en) * 2001-07-12 2003-01-23 University Of Massachusetts IN VIVO PRODUCTION OF SMALL INTERFERING RNAs THAT MEDIATE GENE SILENCING
WO2003023015A2 (en) * 2001-09-13 2003-03-20 California Institute Of Technology Method for expression of small antiviral rna molecules within a cell
WO2003062394A2 (en) * 2002-01-22 2003-07-31 Cold Spring Harbor Laboratory Methods and compositions for rna interference

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4517338A (en) 1983-06-20 1985-05-14 Chiron Corporation Multiple reactor system and method for polynucleotide synthesis
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
FR2646438B1 (fr) * 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
WO1999046388A1 (en) 1998-03-12 1999-09-16 Taisho Pharmaceutical Co., Ltd. Nucleic acid enzyme showing allosteric rna-cleaving activity on target rna
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
ATE526405T1 (de) 1998-03-20 2011-10-15 Commw Scient Ind Res Org Synthetische gene und genetische zusammensetzungen diesen enthaltend
US6261806B1 (en) 1998-08-18 2001-07-17 Biotransplant, Inc. Molecular sequence of swine retrovirus and methods of use
WO2002081628A2 (en) 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
KR20080023768A (ko) 2000-03-30 2008-03-14 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Ｒｎａ 간섭의 ｒｎａ 서열 특이적인 매개체
US20030143597A1 (en) * 2000-12-28 2003-07-31 Finney Robert E. Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis
KR20040023596A (ko) * 2001-05-01 2004-03-18 도꾸리쯔교세이호진 상교기쥬쯔 소고겡뀨죠 신규 맥시자임
AU2002329667A1 (en) 2001-07-30 2003-02-17 Uta Griesenbach Specific inhibition of gene expression by small double stranded rnas
US20030148519A1 (en) 2001-11-14 2003-08-07 Engelke David R. Intracellular expression and delivery of siRNAs in mammalian cells
WO2003056022A2 (en) 2001-12-21 2003-07-10 Oxford Biomedica (Uk) Limited Method for producing a transgenic organism using a lentiviral expression vector such as eiav
KR20040089096A (ko) 2001-12-21 2004-10-20 트란지미, 인크. 렌티바이러스 벡터를 이용한 유전자 변형된 동물을제조하기 위한 방법 및 조성물
GB0130955D0 (en) 2001-12-24 2002-02-13 Cancer Res Ventures Expression system
WO2003064621A2 (en) 2002-02-01 2003-08-07 Ambion, Inc. HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US20030203868A1 (en) * 2002-02-06 2003-10-30 Bushman Frederic D. Inhibition of pathogen replication by RNA interference
US20040045043A1 (en) 2002-05-20 2004-03-04 Finney Robert E. Compositions and methods for generating conditional knockouts
EP3170890A1 (en) 2002-08-21 2017-05-24 Revivicor, Inc. Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase
US20040072769A1 (en) * 2002-09-16 2004-04-15 Yin James Qinwei Methods for design and selection of short double-stranded oligonucleotides, and compounds of gene drugs
CN1276976C (zh) * 2003-10-30 2006-09-27 封江南 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法
EP1582591A3 (en) 2004-02-27 2005-12-21 Bundesrepublik Deutschland vertreten durch das Bundesminsterium für Gesundheit, dieses vertr. durch das Robert-Koch-Institut Sirna and their use for knock down expression of porcine endogenous retrovirus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001068836A2 (en) * 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
WO2003006477A1 (en) * 2001-07-12 2003-01-23 University Of Massachusetts IN VIVO PRODUCTION OF SMALL INTERFERING RNAs THAT MEDIATE GENE SILENCING
WO2003023015A2 (en) * 2001-09-13 2003-03-20 California Institute Of Technology Method for expression of small antiviral rna molecules within a cell
WO2003062394A2 (en) * 2002-01-22 2003-07-31 Cold Spring Harbor Laboratory Methods and compositions for rna interference

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6010075851; ＦＥＢＳ　Ｌｅｔｔ．　２００２　Ｄｅｃ　４；５３２（１-２）：２２７-３０． *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007525192A (ja) * 2003-05-23 2007-09-06 サーナ・セラピューティクス・インコーポレイテッド 化学修飾した低分子干渉核酸（ｓｉＮＡ）を使用する遺伝子発現のＲＮＡ干渉仲介抑制
JP2010528667A (ja) * 2007-06-13 2010-08-26 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション トリの生産形質の改変
JP2013501942A (ja) * 2009-08-14 2013-01-17 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 自閉症を診断及び治療する方法
US9529001B2 (en) 2009-08-14 2016-12-27 The Regents Of The University Of California Methods of diagnosing and treating autism
US10520499B2 (en) 2009-08-14 2019-12-31 The Regents Of The University Of California Methods of diagnosing and treating autism
US11098082B2 (en) 2015-06-26 2021-08-24 The Regents Of The University Of California Antigenic peptides and uses thereof for diagnosing and treating autism
JP2023551513A (ja) * 2020-12-01 2023-12-08 エー・テー・ハー・チューリッヒ 代替プロモータを含むｄｎａ構築物

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