CN103397019A - 干扰rna在生产转基因动物中的用途 - Google Patents

干扰rna在生产转基因动物中的用途 Download PDF

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CN103397019A
CN103397019A CN2013103209599A CN201310320959A CN103397019A CN 103397019 A CN103397019 A CN 103397019A CN 2013103209599 A CN2013103209599 A CN 2013103209599A CN 201310320959 A CN201310320959 A CN 201310320959A CN 103397019 A CN103397019 A CN 103397019A
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gene
cell
irna
sequence
target
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K.维尔斯
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Revivicor Inc
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CN2013103209599A 2003-11-21 2004-11-22 干扰rna在生产转基因动物中的用途 Pending CN103397019A (zh)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104232670A (zh) * 2014-08-25 2014-12-24 中国水产科学研究院黑龙江水产研究所 一种采用鱼类转基因方法构建的基于鲤鱼mstn干涉载体及其构建方法
CN104677866A (zh) * 2014-03-25 2015-06-03 中国科学院大连化学物理研究所 测定CP装载siRNA体系中siRNA释放的方法
CN106918697A (zh) * 2017-02-15 2017-07-04 中国医学科学院北京协和医院 一种预测ra药物疗效的诊断标志物及其应用
CN107760682A (zh) * 2017-10-18 2018-03-06 云南大学 一种可用于分子防治害虫的核酸序列及其应用
CN108513580A (zh) * 2015-10-08 2018-09-07 哈佛学院董事及会员团体 多重基因组编辑
CN110055253A (zh) * 2019-04-28 2019-07-26 复旦大学附属金山医院 一种针对人半胱氨酸蛋白酶抑制剂B的siRNA分子及其应用
CN110628807A (zh) * 2018-05-30 2019-12-31 中国科学院植物研究所 盐角草SePSS蛋白及其编码基因与应用
CN115011601A (zh) * 2022-06-27 2022-09-06 山东大学齐鲁医院 一种干扰JUND表达的shRNA、重组腺相关病毒载体及其应用
US12058986B2 (en) 2017-04-20 2024-08-13 Egenesis, Inc. Method for generating a genetically modified pig with inactivated porcine endogenous retrovirus (PERV) elements
CN118581151A (zh) * 2024-05-29 2024-09-03 中国农业科学院兰州兽医研究所(中国动物卫生与流行病学中心兰州分中心) 一种猪becn1基因敲除细胞的构建方法及其应用
CN120608104A (zh) * 2025-08-12 2025-09-09 中国科学院水生生物研究所 lasp1基因在鱼类抗病毒免疫调控中的功能和应用

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4948163B2 (ja) * 2003-05-23 2012-06-06 サーナ・セラピューティクス・インコーポレイテッド 化学修飾した低分子干渉核酸（ｓｉＮＡ）を使用する遺伝子発現のＲＮＡ干渉仲介抑制
US20050028228A1 (en) * 2003-07-21 2005-02-03 Lifecell Corporation Acellular tissue matrices made from alpa-1,3-galactose-deficient tissue
EP1582591A3 (en) * 2004-02-27 2005-12-21 Bundesrepublik Deutschland vertreten durch das Bundesminsterium für Gesundheit, dieses vertr. durch das Robert-Koch-Institut Sirna and their use for knock down expression of porcine endogenous retrovirus
KR20080025172A (ko) * 2005-06-24 2008-03-19 리전츠 오브 더 유니버스티 오브 미네소타 돼지에서 인간으로의 레트로엘리먼트 전달을 감소시키기위한 사이토신 탈아미노효소의 사용
JP2007104969A (ja) * 2005-10-13 2007-04-26 Bio Think Tank Co Ltd ショートヘアピン型ＲＮＡ（ｓｈＲＮＡ）前駆体を産生するための核酸及びその利用
JP5832721B2 (ja) 2007-03-14 2015-12-16 バイオンシル・エス．アール．エル．Ｂｉｏｎｓｉｌ Ｓ．Ｒ．Ｌ． 上皮腫瘍細胞における薬剤耐性のモジュレータ化合物
CA2691617C (en) * 2007-05-31 2020-07-21 University Of Iowa Research Foundation Reduction of off-target rna interference toxicity
WO2008151364A1 (en) * 2007-06-13 2008-12-18 Commonwealth Scientific And Industrial Research Organisation Modulating production traits in avians
WO2010008562A2 (en) 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
WO2010125117A2 (en) * 2009-04-29 2010-11-04 Ralph Wirtz A method to assess prognosis and to predict therapeutic success in cancer by determining hormone receptor expression levels
DK2464976T3 (en) 2009-08-14 2019-03-04 Univ California Methods for diagnosing and treating autism
US9420770B2 (en) 2009-12-01 2016-08-23 Indiana University Research & Technology Corporation Methods of modulating thrombocytopenia and modified transgenic pigs
EP2585822A4 (en) * 2010-06-24 2014-03-12 BY TARGETED MIR-29 EXPRESSION IN A MOUSE MODELED CHRONIC LYMPHATIC LEUKEMIA
WO2012109667A1 (en) 2011-02-12 2012-08-16 University Of Iowa Research Foundation Therapeutic compounds
EP2698379A4 (en) * 2011-03-18 2015-04-29 Shanghai Inst Biol Sciences INSECTROSIVE PREPARATION AND METHODS BASED ON RNAI TECHNOLOGY
WO2013188358A1 (en) * 2012-06-12 2013-12-19 The General Hospital Corporation Miniature swine transgenic for one or more coagulation factors
DK3065790T3 (da) 2013-11-04 2021-02-01 Lifecell Corp Fremgangsmåder til fjernelse af alpha-galactose
US9523093B2 (en) 2014-05-20 2016-12-20 University Of Iowa Research Foundation Huntington's disease therapeutic compounds
JP6873060B2 (ja) 2015-06-26 2021-05-19 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 自閉症を診断および治療するための抗原性ペプチドおよびその使用
WO2020033456A1 (en) * 2018-08-06 2020-02-13 Nemametrix Inc Homologous recombination reporter construct and uses thereof
US10883084B2 (en) 2018-10-05 2021-01-05 Xenotherapeutics, Inc. Personalized cells, tissues, and organs for transplantation from a humanized, bespoke, designated-pathogen free, (non-human) donor and methods and products relating to same
CA3114681A1 (en) 2018-10-05 2020-04-09 Xenotherapeutics, Inc. Xenotransplantation products and methods
CA3118148A1 (en) * 2018-11-08 2020-05-14 Greenlight Biosciences, Inc. Control of insect infestation
US20220012673A1 (en) * 2020-07-13 2022-01-13 ZenDesk, Inc. Maintaining status information for customer-support agents across multiple channels
CA3196641A1 (en) * 2020-12-01 2022-06-09 Yaakov Benenson Dna constructs comprising alternative promoters
CA3217457A1 (en) * 2021-05-06 2022-11-10 Spyro Mousses Multitargeting rna immunotherapy compositions
EP4347035A4 (en) * 2021-06-01 2025-09-03 Univ Massachusetts CAS9 NICKING-MEDIATED GENE EDITING
WO2023004365A1 (en) * 2021-07-20 2023-01-26 Sio Gene Therapies Inc. Vector constructs for delivery of nucleic acids encoding therapeutic proteasome activator complex subunits and methods of using the same

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4517338A (en) 1983-06-20 1985-05-14 Chiron Corporation Multiple reactor system and method for polynucleotide synthesis
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
EP1063296A1 (en) 1998-03-12 2000-12-27 Taisho Pharmaceutical Co., Ltd Nucleic acid enzyme showing allosteric rna-cleaving activity on target rna
NZ506648A (en) 1998-03-20 2003-08-29 Benitec Australia Ltd Control of gene expression through introduction of synthetic tandem repeats to reduce translation of mRNA
AUPP249298A0 (en) * 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
AU5682999A (en) 1998-08-18 2000-03-14 Biotransplant Incorporated Molecular sequence of swine retrovirus and methods of use
WO2002081628A2 (en) * 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
US20030084471A1 (en) * 2000-03-16 2003-05-01 David Beach Methods and compositions for RNA interference
CA2403397A1 (en) * 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
DK2796553T3 (da) * 2000-03-30 2019-09-30 Whitehead Inst Biomedical Res Rna-sekvensspecifikke formidlere af rna-interferens
US20030143597A1 (en) * 2000-12-28 2003-07-31 Finney Robert E. Methods for making polynucleotide libraries, polynucleotide arrays, and cell libraries for high-throughput genomics analysis
EP1384783A4 (en) * 2001-05-01 2005-04-20 Nat Inst Of Advanced Ind Scien NEW MAXIZYM
EP1412371B1 (en) * 2001-07-12 2016-02-24 University of Massachusetts IN VIVO PRODUCTION OF SMALL INTERFERING RNAs THAT MEDIATE GENE SILENCING
WO2003012052A2 (en) 2001-07-30 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Specific inhibition of gene expression by small double stranded rnas
WO2003022052A1 (en) * 2001-09-13 2003-03-20 California Institute Of Technology Method for expression of small rna molecules within a cell
US20030148519A1 (en) * 2001-11-14 2003-08-07 Engelke David R. Intracellular expression and delivery of siRNAs in mammalian cells
JP2005512598A (ja) 2001-12-21 2005-05-12 オックスフォード バイオメディカ （ユーケー） リミテッド Ｅｉａｖなどのレンチウイルス発現ベクターを使用するトランスジェニック生物の作製方法
KR20040089096A (ko) 2001-12-21 2004-10-20 트란지미, 인크. 렌티바이러스 벡터를 이용한 유전자 변형된 동물을제조하기 위한 방법 및 조성물
GB0130955D0 (en) 2001-12-24 2002-02-13 Cancer Res Ventures Expression system
EP1572902B1 (en) * 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US20030203868A1 (en) * 2002-02-06 2003-10-30 Bushman Frederic D. Inhibition of pathogen replication by RNA interference
US20040045043A1 (en) 2002-05-20 2004-03-04 Finney Robert E. Compositions and methods for generating conditional knockouts
JP2005536228A (ja) 2002-08-21 2005-12-02 レビビコア， インコーポレイテッド 機能的α−１，３−ガラクトシルトランスフェラーゼのいかなる発現をも欠くブタ動物
US20040072769A1 (en) * 2002-09-16 2004-04-15 Yin James Qinwei Methods for design and selection of short double-stranded oligonucleotides, and compounds of gene drugs
CN1276976C (zh) * 2003-10-30 2006-09-27 封江南 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法
EP1582591A3 (en) * 2004-02-27 2005-12-21 Bundesrepublik Deutschland vertreten durch das Bundesminsterium für Gesundheit, dieses vertr. durch das Robert-Koch-Institut Sirna and their use for knock down expression of porcine endogenous retrovirus

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