JP2006507829A - 発現ベクター、異種遺伝子生成物の製造方法およびこのような生成物を高濃度で製造する組換え細胞の選別方法 - Google Patents
発現ベクター、異種遺伝子生成物の製造方法およびこのような生成物を高濃度で製造する組換え細胞の選別方法 Download PDFInfo
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- JP2006507829A JP2006507829A JP2004556182A JP2004556182A JP2006507829A JP 2006507829 A JP2006507829 A JP 2006507829A JP 2004556182 A JP2004556182 A JP 2004556182A JP 2004556182 A JP2004556182 A JP 2004556182A JP 2006507829 A JP2006507829 A JP 2006507829A
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- gene
- protein
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- expression vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10256083A DE10256083A1 (de) | 2002-11-29 | 2002-11-29 | Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen |
PCT/EP2003/013225 WO2004050879A1 (de) | 2002-11-29 | 2003-11-25 | Expressionsvektor, verfahren zur herstellung von heterologen genprodukten und selektionsverfahren für hochproduzierende rekombinante zellen |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2006507829A true JP2006507829A (ja) | 2006-03-09 |
Family
ID=32403676
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2004556182A Pending JP2006507829A (ja) | 2002-11-29 | 2003-11-25 | 発現ベクター、異種遺伝子生成物の製造方法およびこのような生成物を高濃度で製造する組換え細胞の選別方法 |
Country Status (12)
Country | Link |
---|---|
EP (1) | EP1567648A1 (es) |
JP (1) | JP2006507829A (es) |
KR (1) | KR100820035B1 (es) |
CN (1) | CN1717486A (es) |
AR (1) | AR042244A1 (es) |
AU (1) | AU2003292102A1 (es) |
BR (1) | BR0316510A (es) |
CA (1) | CA2507714A1 (es) |
DE (1) | DE10256083A1 (es) |
MX (1) | MXPA05005482A (es) |
TW (1) | TWI321152B (es) |
WO (1) | WO2004050879A1 (es) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009544293A (ja) * | 2006-07-26 | 2009-12-17 | ベーリンガー インゲルハイム ファルマ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 調節核酸配列要素 |
JP2011505859A (ja) * | 2007-12-21 | 2011-03-03 | ノバルティス アーゲー | 哺乳類発現ベクター |
JP2012518992A (ja) * | 2009-02-27 | 2012-08-23 | ノバルティス アーゲー | 2種類の選択マーカーを含む発現ベクター系 |
WO2012161352A1 (ja) * | 2011-05-25 | 2012-11-29 | 国立大学法人岡山大学 | Reic発現アデノウイルスベクター |
JP2014530634A (ja) * | 2011-10-28 | 2014-11-20 | ラボラトワール フランセ ドゥ フラクションマン エ デ バイオテクノロジーズLaboratoire Francais DuFractionnement Et Des Biotechnologies | 発現ベクターにおける転写単位およびその使用 |
JP2021530225A (ja) * | 2018-07-13 | 2021-11-11 | ロンザ リミテッドLonza Limited | 内因性タンパク質のレベルを低下させることによって生物学的産物の産生を改善する方法 |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101239495B1 (ko) * | 2011-01-21 | 2013-03-05 | 경상대학교산학협력단 | αΑ-크리스탈린 유전자를 발현하는 재조합 아데노바이러스 및 이를 이용한 망막혈관 질환의 유전자 치료 |
DK3412684T3 (da) | 2013-07-31 | 2022-07-04 | Novartis Ag | Nye selektionsvektorer og fremgangsmåder til selektering af eukaryotiske værtsceller |
CN104404072B (zh) * | 2014-11-24 | 2018-04-27 | 浙江大学 | 用标记基因预测外源基因表达量及筛选转基因家蚕的方法 |
CN106442685B (zh) * | 2016-10-25 | 2019-03-26 | 山东大学 | 一种快速低成本筛选蛋白质表达条件的方法 |
EP3966332A4 (en) * | 2019-05-07 | 2023-01-25 | Amgen, Inc. | VECTORS AND EXPRESSION SYSTEMS TO PRODUCE RECOMBINANT PROTEINS |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5179017A (en) * | 1980-02-25 | 1993-01-12 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
US4656134A (en) * | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
DE3584341D1 (de) | 1984-08-24 | 1991-11-14 | Upjohn Co | Rekombinante dna-verbindungen und expression von polypeptiden wie tpa. |
US5491084A (en) * | 1993-09-10 | 1996-02-13 | The Trustees Of Columbia University In The City Of New York | Uses of green-fluorescent protein |
ATE400651T1 (de) * | 1993-09-10 | 2008-07-15 | Univ Columbia | Verwendung von grünem fluoreszenzprotein |
DE19539493A1 (de) * | 1995-10-24 | 1997-04-30 | Thomae Gmbh Dr K | Starker homologer Promotor aus Hamster |
EP0961830A1 (en) * | 1997-01-29 | 1999-12-08 | Neurosearch A/S | EXPRESSION VECTORS AND METHODS FOR $i(IN VIVO) EXPRESSION OF THERAPEUTIC POLYPEPTIDES |
ES2257303T3 (es) * | 1999-07-12 | 2006-08-01 | Genentech, Inc. | Vectores de expresion y procedimientos. |
EP1258255A1 (en) * | 2001-05-18 | 2002-11-20 | Boehringer Ingelheim International GmbH | Conjugates of an antibody to CD44 and a maytansinoid |
-
2002
- 2002-11-29 DE DE10256083A patent/DE10256083A1/de not_active Ceased
-
2003
- 2003-11-25 CN CNA2003801044261A patent/CN1717486A/zh active Pending
- 2003-11-25 AU AU2003292102A patent/AU2003292102A1/en not_active Abandoned
- 2003-11-25 CA CA002507714A patent/CA2507714A1/en not_active Abandoned
- 2003-11-25 JP JP2004556182A patent/JP2006507829A/ja active Pending
- 2003-11-25 BR BR0316510-8A patent/BR0316510A/pt not_active Expired - Fee Related
- 2003-11-25 KR KR1020057009693A patent/KR100820035B1/ko not_active IP Right Cessation
- 2003-11-25 EP EP03767642A patent/EP1567648A1/de not_active Withdrawn
- 2003-11-25 WO PCT/EP2003/013225 patent/WO2004050879A1/de active Application Filing
- 2003-11-25 MX MXPA05005482A patent/MXPA05005482A/es not_active Application Discontinuation
- 2003-11-26 TW TW092133164A patent/TWI321152B/zh not_active IP Right Cessation
- 2003-11-28 AR ARP030104395A patent/AR042244A1/es unknown
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009544293A (ja) * | 2006-07-26 | 2009-12-17 | ベーリンガー インゲルハイム ファルマ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 調節核酸配列要素 |
JP2011505859A (ja) * | 2007-12-21 | 2011-03-03 | ノバルティス アーゲー | 哺乳類発現ベクター |
JP2015091238A (ja) * | 2007-12-21 | 2015-05-14 | ノバルティス アーゲー | 哺乳類発現ベクター |
JP2012518992A (ja) * | 2009-02-27 | 2012-08-23 | ノバルティス アーゲー | 2種類の選択マーカーを含む発現ベクター系 |
JP2015109839A (ja) * | 2009-02-27 | 2015-06-18 | ノバルティス アーゲー | 2種類の選択マーカーを含む発現ベクター系 |
WO2012161352A1 (ja) * | 2011-05-25 | 2012-11-29 | 国立大学法人岡山大学 | Reic発現アデノウイルスベクター |
JPWO2012161352A1 (ja) * | 2011-05-25 | 2014-07-31 | 国立大学法人 岡山大学 | Reic発現アデノウイルスベクター |
US9222107B2 (en) | 2011-05-25 | 2015-12-29 | National University Corporation Okayama University | REIC-expressing adenovirus vector |
JP6014029B2 (ja) * | 2011-05-25 | 2016-10-25 | 国立大学法人 岡山大学 | Reic発現アデノウイルスベクター |
JP2014530634A (ja) * | 2011-10-28 | 2014-11-20 | ラボラトワール フランセ ドゥ フラクションマン エ デ バイオテクノロジーズLaboratoire Francais DuFractionnement Et Des Biotechnologies | 発現ベクターにおける転写単位およびその使用 |
JP2021530225A (ja) * | 2018-07-13 | 2021-11-11 | ロンザ リミテッドLonza Limited | 内因性タンパク質のレベルを低下させることによって生物学的産物の産生を改善する方法 |
Also Published As
Publication number | Publication date |
---|---|
EP1567648A1 (de) | 2005-08-31 |
TW200502388A (en) | 2005-01-16 |
AR042244A1 (es) | 2005-06-15 |
CN1717486A (zh) | 2006-01-04 |
CA2507714A1 (en) | 2004-06-17 |
AU2003292102A1 (en) | 2004-06-23 |
BR0316510A (pt) | 2005-10-04 |
KR20050085203A (ko) | 2005-08-29 |
TWI321152B (en) | 2010-03-01 |
KR100820035B1 (ko) | 2008-04-10 |
WO2004050879A1 (de) | 2004-06-17 |
DE10256083A1 (de) | 2004-08-12 |
MXPA05005482A (es) | 2005-07-25 |
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