TW200502388A - Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products - Google Patents

Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products

Info

Publication number
TW200502388A
TW200502388A TW092133164A TW92133164A TW200502388A TW 200502388 A TW200502388 A TW 200502388A TW 092133164 A TW092133164 A TW 092133164A TW 92133164 A TW92133164 A TW 92133164A TW 200502388 A TW200502388 A TW 200502388A
Authority
TW
Taiwan
Prior art keywords
expression vector
products
selection
heterologous gene
production
Prior art date
Application number
TW092133164A
Other languages
Chinese (zh)
Other versions
TWI321152B (en
Inventor
Barbara Enenkel
Juergen Fieder
Ralf Otto
Stefanos Grammatikos
Original Assignee
Boehringer Ingelheim Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma filed Critical Boehringer Ingelheim Pharma
Publication of TW200502388A publication Critical patent/TW200502388A/en
Application granted granted Critical
Publication of TWI321152B publication Critical patent/TWI321152B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An expression vector for eukaryotic cells comprising a gene which codes for a protein of interest, functionally linked to a hamster-ubiquitin/S27a-promoter and a gene which codes for a fluorescent protein. Preferably the expression vector also contains an amplifiable selectable marker gene. The invention also describes host cells, preferably mammalian cells, which have been transfected with the expression vector, processes for producing heterologous gene products and a method of selecting high-producing cells.
TW092133164A 2002-11-29 2003-11-26 Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products TWI321152B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10256083A DE10256083A1 (en) 2002-11-29 2002-11-29 Expression vector, process for the production of heterologous gene products and selection process for high-producing recombinant cells

Publications (2)

Publication Number Publication Date
TW200502388A true TW200502388A (en) 2005-01-16
TWI321152B TWI321152B (en) 2010-03-01

Family

ID=32403676

Family Applications (1)

Application Number Title Priority Date Filing Date
TW092133164A TWI321152B (en) 2002-11-29 2003-11-26 Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products

Country Status (12)

Country Link
EP (1) EP1567648A1 (en)
JP (1) JP2006507829A (en)
KR (1) KR100820035B1 (en)
CN (1) CN1717486A (en)
AR (1) AR042244A1 (en)
AU (1) AU2003292102A1 (en)
BR (1) BR0316510A (en)
CA (1) CA2507714A1 (en)
DE (1) DE10256083A1 (en)
MX (1) MXPA05005482A (en)
TW (1) TWI321152B (en)
WO (1) WO2004050879A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124760A1 (en) * 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
ES2585502T3 (en) * 2007-12-21 2016-10-06 Novartis Ag Mammalian Expression Vector
JP5801207B2 (en) 2009-02-27 2015-10-28 ノバルティス アーゲー Expression vector system containing two types of selectable markers
KR101239495B1 (en) * 2011-01-21 2013-03-05 경상대학교산학협력단 Recombinant adenovirus expressing αA-crystallin gene and gene therapy using the same for the prevention and treatment of retinal vascular diseases
TWI545196B (en) 2011-05-25 2016-08-11 國立大學法人岡山大學 An adenovirus vector which expresses reic
FR2981946B1 (en) 2011-10-28 2015-02-20 Lfb Biotechnologies TRANSCRIPTION UNITS AND THEIR USE IN EXPRESSION VECTORS (YB2 / 0)
LT3412684T (en) 2013-07-31 2022-07-11 Novartis Ag Novel selection vectors and methods of selecting eukaryotic host cells
CN104404072B (en) * 2014-11-24 2018-04-27 浙江大学 Exogenous gene expression amount and the method for screening transgenic silkworm are predicted with marker gene
CN106442685B (en) * 2016-10-25 2019-03-26 山东大学 A kind of method of fast and low-cost screening protein expression condition
SG11202013191TA (en) * 2018-07-13 2021-01-28 Lonza Ag Methods for improving production of biological products by reducing the level of endogenous protein
WO2020227206A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179017A (en) * 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0173552B1 (en) 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
AU694745B2 (en) * 1993-09-10 1998-07-30 Trustees Of Columbia University In The City Of New York, The Uses of green fluorescent protein
DE19539493A1 (en) * 1995-10-24 1997-04-30 Thomae Gmbh Dr K Strong homologous promoter from hamster
WO1998032869A1 (en) * 1997-01-29 1998-07-30 Neurosearch A/S Expression vectors and methods for in vivo expression of therapeutic polypeptides
ES2257303T3 (en) * 1999-07-12 2006-08-01 Genentech, Inc. VECTORS OF EXPRESSION AND PROCEDURES.
EP1258255A1 (en) * 2001-05-18 2002-11-20 Boehringer Ingelheim International GmbH Conjugates of an antibody to CD44 and a maytansinoid

Also Published As

Publication number Publication date
JP2006507829A (en) 2006-03-09
BR0316510A (en) 2005-10-04
AR042244A1 (en) 2005-06-15
KR20050085203A (en) 2005-08-29
EP1567648A1 (en) 2005-08-31
WO2004050879A1 (en) 2004-06-17
CN1717486A (en) 2006-01-04
MXPA05005482A (en) 2005-07-25
CA2507714A1 (en) 2004-06-17
KR100820035B1 (en) 2008-04-10
AU2003292102A1 (en) 2004-06-23
TWI321152B (en) 2010-03-01
DE10256083A1 (en) 2004-08-12

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Legal Events

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MM4A Annulment or lapse of patent due to non-payment of fees