AR042244A1 - Vector de expresion, procedimiento para la preparacion de productos genicos heterologos y procedimiento de seleccion para celulas recombinantes de alta produccion - Google Patents

Vector de expresion, procedimiento para la preparacion de productos genicos heterologos y procedimiento de seleccion para celulas recombinantes de alta produccion

Info

Publication number
AR042244A1
AR042244A1 ARP030104395A ARP030104395A AR042244A1 AR 042244 A1 AR042244 A1 AR 042244A1 AR P030104395 A ARP030104395 A AR P030104395A AR P030104395 A ARP030104395 A AR P030104395A AR 042244 A1 AR042244 A1 AR 042244A1
Authority
AR
Argentina
Prior art keywords
expression vector
procedure
cells
preparation
products
Prior art date
Application number
ARP030104395A
Other languages
English (en)
Inventor
Jurgen Fieder
Enenkel Barbara Dr
Otto Ralf Dr
Grammatikos Stefanos Dr
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of AR042244A1 publication Critical patent/AR042244A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un vector de expresión para células eucarióticas que abarca un gen que codifica una proteína de interés, en enlace funcional con un promotor ubiquitina/S27a del hámster y un gen que codifica una proteína fluorescente. Preferiblemente, el vector de expresión contiene, además, un gen marcador de la selección amplificable. Se describen, además, células huésped transfectadas con el vector de expresión, preferiblemente células de mamífero, procedimientos para la preparación de productos génicos heterólogos y un procedimiento para la selección de células de alta producción. Reivindicación 7: Vector de expresión según una de las reivindicaciones 1 a 6, caracterizado porque el gen marcador de la selección amplificable codifica dihidrofolato- reductasa (DHFR) o una proteína de fusión a base de la proteína fluorescente y DHFR. Reivindicación 8: Vector de expresión según una de las reivindicaciones 3 a 7, caracterizada porque el intensificador es un intensificador de CMV o SV40. Reivindicación 12: Célula huésped según la reivindicación 11, caracterizada porque se trata de una célula de mamífero, preferiblemente una célula CHO. Reivindicación 16: Procedimiento según la reivindicación 15, caracterizado porque la proteína heterómera es un anticuerpo.
ARP030104395A 2002-11-29 2003-11-28 Vector de expresion, procedimiento para la preparacion de productos genicos heterologos y procedimiento de seleccion para celulas recombinantes de alta produccion AR042244A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10256083A DE10256083A1 (de) 2002-11-29 2002-11-29 Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen

Publications (1)

Publication Number Publication Date
AR042244A1 true AR042244A1 (es) 2005-06-15

Family

ID=32403676

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030104395A AR042244A1 (es) 2002-11-29 2003-11-28 Vector de expresion, procedimiento para la preparacion de productos genicos heterologos y procedimiento de seleccion para celulas recombinantes de alta produccion

Country Status (12)

Country Link
EP (1) EP1567648A1 (es)
JP (1) JP2006507829A (es)
KR (1) KR100820035B1 (es)
CN (1) CN1717486A (es)
AR (1) AR042244A1 (es)
AU (1) AU2003292102A1 (es)
BR (1) BR0316510A (es)
CA (1) CA2507714A1 (es)
DE (1) DE10256083A1 (es)
MX (1) MXPA05005482A (es)
TW (1) TWI321152B (es)
WO (1) WO2004050879A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124760A1 (en) * 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
US20110045536A1 (en) * 2007-12-21 2011-02-24 Novartis Ag Mammalian expression vector
PT2401377T (pt) * 2009-02-27 2016-08-18 Novartis Ag Sistema de vectores de expressão compreendendo dois marcadores de selecção
KR101239495B1 (ko) * 2011-01-21 2013-03-05 경상대학교산학협력단 αΑ-크리스탈린 유전자를 발현하는 재조합 아데노바이러스 및 이를 이용한 망막혈관 질환의 유전자 치료
TWI545196B (zh) * 2011-05-25 2016-08-11 國立大學法人岡山大學 Ｒｅｉｃ表現腺病毒載體
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
RU2716977C2 (ru) 2013-07-31 2020-03-17 Новартис Аг Новые селективные векторы и способы селекции эукариотических клеток-хозяев
CN104404072B (zh) * 2014-11-24 2018-04-27 浙江大学 用标记基因预测外源基因表达量及筛选转基因家蚕的方法
CN106442685B (zh) * 2016-10-25 2019-03-26 山东大学 一种快速低成本筛选蛋白质表达条件的方法
CA3137457A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179017A (en) * 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0173552B1 (en) 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
CA2169298A1 (en) * 1993-09-10 1995-03-16 Martin Chalfie Uses of green fluorescent protein
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
DE19539493A1 (de) 1995-10-24 1997-04-30 Thomae Gmbh Dr K Starker homologer Promotor aus Hamster
EP0961830A1 (en) * 1997-01-29 1999-12-08 Neurosearch A/S EXPRESSION VECTORS AND METHODS FOR $i(IN VIVO) EXPRESSION OF THERAPEUTIC POLYPEPTIDES
CA2385102A1 (en) * 1999-07-12 2001-01-18 Genentech, Inc. Expression vectors and methods
EP1258255A1 (en) * 2001-05-18 2002-11-20 Boehringer Ingelheim International GmbH Conjugates of an antibody to CD44 and a maytansinoid

Also Published As

Publication number Publication date
AU2003292102A1 (en) 2004-06-23
WO2004050879A1 (de) 2004-06-17
TWI321152B (en) 2010-03-01
CA2507714A1 (en) 2004-06-17
KR20050085203A (ko) 2005-08-29
MXPA05005482A (es) 2005-07-25
TW200502388A (en) 2005-01-16
BR0316510A (pt) 2005-10-04
JP2006507829A (ja) 2006-03-09
CN1717486A (zh) 2006-01-04
EP1567648A1 (de) 2005-08-31
KR100820035B1 (ko) 2008-04-10
DE10256083A1 (de) 2004-08-12

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