CN1717486A - 表达载体、异源基因产物的制备方法以及高产重组细胞的选择方法 - Google Patents

表达载体、异源基因产物的制备方法以及高产重组细胞的选择方法 Download PDF

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Publication number
CN1717486A
CN1717486A CNA2003801044261A CN200380104426A CN1717486A CN 1717486 A CN1717486 A CN 1717486A CN A2003801044261 A CNA2003801044261 A CN A2003801044261A CN 200380104426 A CN200380104426 A CN 200380104426A CN 1717486 A CN1717486 A CN 1717486A
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China
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gene
cell
expression vector
host cell
protein
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Pending
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CNA2003801044261A
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English (en)
Chinese (zh)
Inventor
巴巴拉·埃南克尔
于尔根·菲德
拉尔夫·奥托
斯蒂法诺斯·格拉马蒂科斯
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Boehringer Ingelheim Pharma GmbH and Co KG
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Boehringer Ingelheim Pharma GmbH and Co KG
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Publication of CN1717486A publication Critical patent/CN1717486A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CNA2003801044261A 2002-11-29 2003-11-25 表达载体、异源基因产物的制备方法以及高产重组细胞的选择方法 Pending CN1717486A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10256083A DE10256083A1 (de) 2002-11-29 2002-11-29 Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen
DE10256083.8 2002-11-29

Publications (1)

Publication Number Publication Date
CN1717486A true CN1717486A (zh) 2006-01-04

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ID=32403676

Family Applications (1)

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CNA2003801044261A Pending CN1717486A (zh) 2002-11-29 2003-11-25 表达载体、异源基因产物的制备方法以及高产重组细胞的选择方法

Country Status (12)

Country Link
EP (1) EP1567648A1 (es)
JP (1) JP2006507829A (es)
KR (1) KR100820035B1 (es)
CN (1) CN1717486A (es)
AR (1) AR042244A1 (es)
AU (1) AU2003292102A1 (es)
BR (1) BR0316510A (es)
CA (1) CA2507714A1 (es)
DE (1) DE10256083A1 (es)
MX (1) MXPA05005482A (es)
TW (1) TWI321152B (es)
WO (1) WO2004050879A1 (es)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101517085B (zh) * 2006-07-26 2013-08-07 贝林格尔英格海姆法玛两合公司 调控性核酸元件
CN104404072A (zh) * 2014-11-24 2015-03-11 浙江大学 用标记基因预测外源基因表达量及筛选转基因生物的方法
CN101903523B (zh) * 2007-12-21 2016-05-18 诺华股份有限公司 哺乳动物表达载体
CN106442685A (zh) * 2016-10-25 2017-02-22 山东大学 一种快速低成本筛选蛋白质表达条件的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG173456A1 (en) 2009-02-27 2011-09-29 Novartis Ag Expression vector system comprising two selection markers
KR101239495B1 (ko) * 2011-01-21 2013-03-05 경상대학교산학협력단 αΑ-크리스탈린 유전자를 발현하는 재조합 아데노바이러스 및 이를 이용한 망막혈관 질환의 유전자 치료
CN103562386A (zh) * 2011-05-25 2014-02-05 国立大学法人冈山大学 Reic表达腺病毒载体
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
EP3412684B1 (en) 2013-07-31 2022-04-06 Novartis AG Novel selection vectors and methods of selecting eukaryotic host cells
CN112424339A (zh) * 2018-07-13 2021-02-26 隆萨有限公司 用于通过降低内源性蛋白质的水平来改善生物产物的生产的方法
WO2020227206A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179017A (en) * 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0173552B1 (en) 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
ATE400651T1 (de) * 1993-09-10 2008-07-15 Univ Columbia Verwendung von grünem fluoreszenzprotein
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
DE19539493A1 (de) * 1995-10-24 1997-04-30 Thomae Gmbh Dr K Starker homologer Promotor aus Hamster
EP0961830A1 (en) * 1997-01-29 1999-12-08 Neurosearch A/S EXPRESSION VECTORS AND METHODS FOR $i(IN VIVO) EXPRESSION OF THERAPEUTIC POLYPEPTIDES
ATE317011T1 (de) * 1999-07-12 2006-02-15 Genentech Inc Expressionsvektoren und anwendungsmethoden
EP1258255A1 (en) * 2001-05-18 2002-11-20 Boehringer Ingelheim International GmbH Conjugates of an antibody to CD44 and a maytansinoid

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101517085B (zh) * 2006-07-26 2013-08-07 贝林格尔英格海姆法玛两合公司 调控性核酸元件
CN101903523B (zh) * 2007-12-21 2016-05-18 诺华股份有限公司 哺乳动物表达载体
CN104404072A (zh) * 2014-11-24 2015-03-11 浙江大学 用标记基因预测外源基因表达量及筛选转基因生物的方法
CN104404072B (zh) * 2014-11-24 2018-04-27 浙江大学 用标记基因预测外源基因表达量及筛选转基因家蚕的方法
CN106442685A (zh) * 2016-10-25 2017-02-22 山东大学 一种快速低成本筛选蛋白质表达条件的方法

Also Published As

Publication number Publication date
CA2507714A1 (en) 2004-06-17
EP1567648A1 (de) 2005-08-31
KR20050085203A (ko) 2005-08-29
JP2006507829A (ja) 2006-03-09
AU2003292102A1 (en) 2004-06-23
WO2004050879A1 (de) 2004-06-17
KR100820035B1 (ko) 2008-04-10
TWI321152B (en) 2010-03-01
TW200502388A (en) 2005-01-16
DE10256083A1 (de) 2004-08-12
MXPA05005482A (es) 2005-07-25
AR042244A1 (es) 2005-06-15
BR0316510A (pt) 2005-10-04

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