KR100820035B1 - 발현 벡터, 이종성 유전자 생성물의 제조 방법 및 고생산성재조합 세포의 선별 방법 - Google Patents

발현 벡터, 이종성 유전자 생성물의 제조 방법 및 고생산성재조합 세포의 선별 방법 Download PDF

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KR100820035B1
KR100820035B1 KR1020057009693A KR20057009693A KR100820035B1 KR 100820035 B1 KR100820035 B1 KR 100820035B1 KR 1020057009693 A KR1020057009693 A KR 1020057009693A KR 20057009693 A KR20057009693 A KR 20057009693A KR 100820035 B1 KR100820035 B1 KR 100820035B1
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gene
cells
delete delete
cell
protein
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KR1020057009693A
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Korean (ko)
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KR20050085203A (ko
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바르바라 에넨켈
위르겐 피더
랄프 오토
슈테파노스 그라마티코스
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베링거 잉겔하임 파르마 게엠베하 운트 코 카게
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020057009693A 2002-11-29 2003-11-25 발현 벡터, 이종성 유전자 생성물의 제조 방법 및 고생산성재조합 세포의 선별 방법 KR100820035B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10256083A DE10256083A1 (de) 2002-11-29 2002-11-29 Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen
DE10256083.8 2002-11-29

Publications (2)

Publication Number Publication Date
KR20050085203A KR20050085203A (ko) 2005-08-29
KR100820035B1 true KR100820035B1 (ko) 2008-04-10

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KR1020057009693A KR100820035B1 (ko) 2002-11-29 2003-11-25 발현 벡터, 이종성 유전자 생성물의 제조 방법 및 고생산성재조합 세포의 선별 방법

Country Status (12)

Country Link
EP (1) EP1567648A1 (es)
JP (1) JP2006507829A (es)
KR (1) KR100820035B1 (es)
CN (1) CN1717486A (es)
AR (1) AR042244A1 (es)
AU (1) AU2003292102A1 (es)
BR (1) BR0316510A (es)
CA (1) CA2507714A1 (es)
DE (1) DE10256083A1 (es)
MX (1) MXPA05005482A (es)
TW (1) TWI321152B (es)
WO (1) WO2004050879A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124760A1 (en) * 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
LT3075858T (lt) * 2007-12-21 2020-04-27 Novartis Ag Žinduolių raiškos vektorius
CN102333871B (zh) 2009-02-27 2013-12-04 诺瓦提斯公司 包含两个选择标记的表达载体系统
KR101239495B1 (ko) * 2011-01-21 2013-03-05 경상대학교산학협력단 αΑ-크리스탈린 유전자를 발현하는 재조합 아데노바이러스 및 이를 이용한 망막혈관 질환의 유전자 치료
TWI545196B (zh) * 2011-05-25 2016-08-11 國立大學法人岡山大學 Ｒｅｉｃ表現腺病毒載體
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
EP3027646B1 (en) 2013-07-31 2018-06-27 Novartis AG Novel selection vectors and methods of selecting eukaryotic host cells
CN104404072B (zh) * 2014-11-24 2018-04-27 浙江大学 用标记基因预测外源基因表达量及筛选转基因家蚕的方法
CN106442685B (zh) * 2016-10-25 2019-03-26 山东大学 一种快速低成本筛选蛋白质表达条件的方法
KR20210030949A (ko) * 2018-07-13 2021-03-18 론자 리미티드 내인성 단백질의 수준을 감소시켜 생물학적 생성물의 생산을 개선하는 방법
WO2020227206A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997015664A1 (de) * 1995-10-24 1997-05-01 Dr. Karl Thomae Gmbh Starker homologer promotor aus hamster
WO2001004306A1 (en) * 1999-07-12 2001-01-18 Genentech, Inc. Expression vectors and methods

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179017A (en) * 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0173552B1 (en) 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
KR960705209A (ko) * 1993-09-10 1996-10-09 잭 엠. 그랜노위츠 녹색 형광 단백의 용도
EP0961830A1 (en) * 1997-01-29 1999-12-08 Neurosearch A/S EXPRESSION VECTORS AND METHODS FOR $i(IN VIVO) EXPRESSION OF THERAPEUTIC POLYPEPTIDES
EP1258255A1 (en) * 2001-05-18 2002-11-20 Boehringer Ingelheim International GmbH Conjugates of an antibody to CD44 and a maytansinoid

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997015664A1 (de) * 1995-10-24 1997-05-01 Dr. Karl Thomae Gmbh Starker homologer promotor aus hamster
WO2001004306A1 (en) * 1999-07-12 2001-01-18 Genentech, Inc. Expression vectors and methods

Also Published As

Publication number Publication date
WO2004050879A1 (de) 2004-06-17
KR20050085203A (ko) 2005-08-29
AU2003292102A1 (en) 2004-06-23
CN1717486A (zh) 2006-01-04
JP2006507829A (ja) 2006-03-09
MXPA05005482A (es) 2005-07-25
TW200502388A (en) 2005-01-16
DE10256083A1 (de) 2004-08-12
EP1567648A1 (de) 2005-08-31
TWI321152B (en) 2010-03-01
BR0316510A (pt) 2005-10-04
AR042244A1 (es) 2005-06-15
CA2507714A1 (en) 2004-06-17

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