CA2507714A1 - Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products - Google Patents

Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products Download PDF

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Publication number
CA2507714A1
CA2507714A1 CA002507714A CA2507714A CA2507714A1 CA 2507714 A1 CA2507714 A1 CA 2507714A1 CA 002507714 A CA002507714 A CA 002507714A CA 2507714 A CA2507714 A CA 2507714A CA 2507714 A1 CA2507714 A1 CA 2507714A1
Authority
CA
Canada
Prior art keywords
gene
protein
cells
expression
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002507714A
Other languages
English (en)
French (fr)
Inventor
Barbara Enenkel
Juergen Fieder
Ralf Otto
Stefanos Grammatikos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2507714A1 publication Critical patent/CA2507714A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002507714A 2002-11-29 2003-11-25 Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products Abandoned CA2507714A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10256083A DE10256083A1 (de) 2002-11-29 2002-11-29 Expressionsvektor, Verfahren zur Herstellung von heterologen Genprodukten und Selektionsverfahren für hochproduzierende rekombinante Zellen
DE10256083.8 2002-11-29
PCT/EP2003/013225 WO2004050879A1 (de) 2002-11-29 2003-11-25 Expressionsvektor, verfahren zur herstellung von heterologen genprodukten und selektionsverfahren für hochproduzierende rekombinante zellen

Publications (1)

Publication Number Publication Date
CA2507714A1 true CA2507714A1 (en) 2004-06-17

Family

ID=32403676

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002507714A Abandoned CA2507714A1 (en) 2002-11-29 2003-11-25 Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products

Country Status (12)

Country Link
EP (1) EP1567648A1 (es)
JP (1) JP2006507829A (es)
KR (1) KR100820035B1 (es)
CN (1) CN1717486A (es)
AR (1) AR042244A1 (es)
AU (1) AU2003292102A1 (es)
BR (1) BR0316510A (es)
CA (1) CA2507714A1 (es)
DE (1) DE10256083A1 (es)
MX (1) MXPA05005482A (es)
TW (1) TWI321152B (es)
WO (1) WO2004050879A1 (es)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9045776B2 (en) 2006-07-26 2015-06-02 Boehringer Ingelheim Pharma Gmbh & Co. Kg Regulatory nucleic acid elements
US9534226B2 (en) 2009-02-27 2017-01-03 Novartis Ag Expression vector system comprising two selection markers
US11807855B2 (en) 2013-07-31 2023-11-07 Novartis Ag Selection vectors and methods of selecting eukaryotic host cells

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2227546T (pt) 2007-12-21 2016-08-12 Novartis Ag Vector de expressão em mamíferos
KR101239495B1 (ko) * 2011-01-21 2013-03-05 경상대학교산학협력단 αΑ-크리스탈린 유전자를 발현하는 재조합 아데노바이러스 및 이를 이용한 망막혈관 질환의 유전자 치료
CN103562386A (zh) * 2011-05-25 2014-02-05 国立大学法人冈山大学 Reic表达腺病毒载体
FR2981946B1 (fr) * 2011-10-28 2015-02-20 Lfb Biotechnologies Unites de transcription et leur utilisation dans des vecteurs d'expression (yb2/0)
CN104404072B (zh) * 2014-11-24 2018-04-27 浙江大学 用标记基因预测外源基因表达量及筛选转基因家蚕的方法
CN106442685B (zh) * 2016-10-25 2019-03-26 山东大学 一种快速低成本筛选蛋白质表达条件的方法
CN112424339A (zh) * 2018-07-13 2021-02-26 隆萨有限公司 用于通过降低内源性蛋白质的水平来改善生物产物的生产的方法
WO2020227206A1 (en) * 2019-05-07 2020-11-12 Amgen Inc. Vectors and expression systems for producing recombinant proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5179017A (en) * 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
EP0173552B1 (en) 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
ATE400651T1 (de) * 1993-09-10 2008-07-15 Univ Columbia Verwendung von grünem fluoreszenzprotein
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
DE19539493A1 (de) * 1995-10-24 1997-04-30 Thomae Gmbh Dr K Starker homologer Promotor aus Hamster
EP0961830A1 (en) * 1997-01-29 1999-12-08 Neurosearch A/S EXPRESSION VECTORS AND METHODS FOR $i(IN VIVO) EXPRESSION OF THERAPEUTIC POLYPEPTIDES
ATE317011T1 (de) * 1999-07-12 2006-02-15 Genentech Inc Expressionsvektoren und anwendungsmethoden
EP1258255A1 (en) * 2001-05-18 2002-11-20 Boehringer Ingelheim International GmbH Conjugates of an antibody to CD44 and a maytansinoid

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9045776B2 (en) 2006-07-26 2015-06-02 Boehringer Ingelheim Pharma Gmbh & Co. Kg Regulatory nucleic acid elements
US9708626B2 (en) 2006-07-26 2017-07-18 Boehringer Ingelheim International Gmbh Regulatory nucleic acid elements
US9534226B2 (en) 2009-02-27 2017-01-03 Novartis Ag Expression vector system comprising two selection markers
US9994866B2 (en) 2009-02-27 2018-06-12 Novartis Ag Expression vector system comprising two selection markers
US11174494B2 (en) 2009-02-27 2021-11-16 Novartis Ag Expression vector system comprising two selection markers
US11807855B2 (en) 2013-07-31 2023-11-07 Novartis Ag Selection vectors and methods of selecting eukaryotic host cells

Also Published As

Publication number Publication date
EP1567648A1 (de) 2005-08-31
KR20050085203A (ko) 2005-08-29
CN1717486A (zh) 2006-01-04
JP2006507829A (ja) 2006-03-09
AU2003292102A1 (en) 2004-06-23
WO2004050879A1 (de) 2004-06-17
KR100820035B1 (ko) 2008-04-10
TWI321152B (en) 2010-03-01
TW200502388A (en) 2005-01-16
DE10256083A1 (de) 2004-08-12
MXPA05005482A (es) 2005-07-25
AR042244A1 (es) 2005-06-15
BR0316510A (pt) 2005-10-04

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20121126