JP2005245453A5 - - Google Patents

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Publication number
JP2005245453A5
JP2005245453A5 JP2005080273A JP2005080273A JP2005245453A5 JP 2005245453 A5 JP2005245453 A5 JP 2005245453A5 JP 2005080273 A JP2005080273 A JP 2005080273A JP 2005080273 A JP2005080273 A JP 2005080273A JP 2005245453 A5 JP2005245453 A5 JP 2005245453A5
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JP
Japan
Prior art keywords
primer
template polynucleotide
codon
degenerate
sequence
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2005080273A
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English (en)
Japanese (ja)
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JP2005245453A (ja
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Priority claimed from US09/246,178 external-priority patent/US6171820B1/en
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Publication of JP2005245453A publication Critical patent/JP2005245453A/ja
Publication of JP2005245453A5 publication Critical patent/JP2005245453A5/ja
Pending legal-status Critical Current

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JP2005080273A 1999-02-04 2005-03-18 遺伝的ワクチンおよび酵素の非確率論的生成 Pending JP2005245453A (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/246,178 US6171820B1 (en) 1995-12-07 1999-02-04 Saturation mutagenesis in directed evolution

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2000597406A Division JP2003524392A (ja) 1999-02-04 2000-02-04 遺伝的ワクチンおよび酵素の非確率論的生成

Publications (2)

Publication Number Publication Date
JP2005245453A JP2005245453A (ja) 2005-09-15
JP2005245453A5 true JP2005245453A5 (enExample) 2007-03-22

Family

ID=22929614

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2000597406A Pending JP2003524392A (ja) 1999-02-04 2000-02-04 遺伝的ワクチンおよび酵素の非確率論的生成
JP2005080273A Pending JP2005245453A (ja) 1999-02-04 2005-03-18 遺伝的ワクチンおよび酵素の非確率論的生成

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2000597406A Pending JP2003524392A (ja) 1999-02-04 2000-02-04 遺伝的ワクチンおよび酵素の非確率論的生成

Country Status (5)

Country Link
US (1) US6171820B1 (enExample)
JP (2) JP2003524392A (enExample)
AU (1) AU3483900A (enExample)
CA (1) CA2325351C (enExample)
WO (1) WO2000046344A2 (enExample)

Families Citing this family (175)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20030219752A1 (en) * 1995-12-07 2003-11-27 Diversa Corporation Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6764835B2 (en) * 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6740506B2 (en) * 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE69835360T2 (de) 1997-01-17 2007-08-16 Maxygen, Inc., Redwood City EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
IL135776A0 (en) 1997-10-24 2001-05-20 Life Technologies Inc Recombinational cloning using nucleic acids having recombination sites
JP3712255B2 (ja) 1997-12-08 2005-11-02 カリフォルニア・インスティチュート・オブ・テクノロジー ポリヌクレオチドおよびポリペプチド配列を生成するための方法
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
US7390619B1 (en) * 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
WO1999057128A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US6991922B2 (en) * 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US20030092023A1 (en) * 1998-08-12 2003-05-15 Daniel Dupret Method of shuffling polynucleotides using templates
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
EP1141275B1 (en) * 1999-01-05 2009-08-12 Trustees Of Boston University Improved nucleic acid cloning
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
AU2415200A (en) * 1999-01-18 2000-08-01 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
JP3399518B2 (ja) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション 半導体構造およびその製造方法
AU3391900A (en) 1999-03-05 2000-09-21 Maxygen, Inc. Encryption of traits using split gene sequences
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
AU1456101A (en) 1999-11-03 2001-05-14 Maxygen, Inc. Antibody diversity generation
US7115712B1 (en) * 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
EP1276900A2 (en) * 2000-01-11 2003-01-22 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU2001287273A1 (en) * 2000-03-24 2001-10-08 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
EP1297146A2 (en) * 2000-06-23 2003-04-02 Maxygen, Inc. Novel chimeric promoters
JP2004513878A (ja) 2000-06-23 2004-05-13 マキシジェン, インコーポレイテッド 新規同時刺激分子
WO2002004629A2 (en) * 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
WO2002008408A2 (en) * 2000-07-21 2002-01-31 Trustees Of Boston University Modular vector systems
US7435562B2 (en) * 2000-07-21 2008-10-14 Modular Genetics, Inc. Modular vector systems
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
CA2421059A1 (en) * 2000-08-24 2002-02-28 Maxygen, Inc. Constructs and their use in metabolic pathway engineering
JP2002083691A (ja) * 2000-09-06 2002-03-22 Sharp Corp アクティブマトリックス駆動型有機led表示装置及びその製造方法
WO2005012515A2 (en) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US6958213B2 (en) * 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
WO2002079468A2 (en) * 2001-02-02 2002-10-10 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
US20030036854A1 (en) * 2001-02-06 2003-02-20 The Penn State Research Foundation Apparatus and method for designing proteins and protein libraries
EP1277835A1 (en) * 2001-07-19 2003-01-22 Libragen Methods of creating genetic diversity
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US20030129203A1 (en) * 2001-08-27 2003-07-10 Nautilus Biotech S.A. Mutant recombinant adeno-associated viruses
ES2561985T3 (es) 2001-10-10 2016-03-01 Ratiopharm Gmbh Remodelación y glicoconjugación de anticuerpos
AU2002364518A1 (en) * 2001-12-03 2003-06-17 Diversa Corporation Chromosomal saturation mutagenesis
TWI262083B (en) * 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
US20040009498A1 (en) * 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
AU2003213108A1 (en) * 2002-02-15 2003-09-09 Verenium Corporation Chimeric cannulae proteins, nucleic acids encoding them and methods for making and using them
AU2003217716A1 (en) * 2002-02-25 2003-09-09 Cabot Corporation Custom ligand design for biomolecular filtration and purification for bioseperation
US20030224404A1 (en) * 2002-02-25 2003-12-04 Manuel Vega High throughput directed evolution of nucleic acids by rational mutagenesis
SI2315145T1 (sl) 2002-03-01 2016-03-31 Codexis Mayflower Holdings, Llc Postopki, sistemi in programska oprema za identificiranje funkcionalnih biomolekul
US20040132101A1 (en) 2002-09-27 2004-07-08 Xencor Optimized Fc variants and methods for their generation
US20030171543A1 (en) * 2002-03-05 2003-09-11 Bott Richard R. High throughput mutagenesis screening method
US20030199068A1 (en) * 2002-03-05 2003-10-23 Bott Richard R. High throughput mutagenesis screening method
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION
EA010903B1 (ru) 2002-04-19 2008-12-30 Дайверса Корпорейшн Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7262012B2 (en) * 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
WO2004009768A2 (en) * 2002-07-18 2004-01-29 Invitrogen Corporation Viral vectors containing recombination sites
EP1546349A1 (en) * 2002-08-02 2005-06-29 Aromagen Corporation Methods for making (-) -menthol and oxygenated menthane compounds
EP1576151A4 (en) 2002-08-06 2006-05-17 Verdia Inc AP1-amine oxidase VARIATIONS
US7611700B2 (en) 2002-09-09 2009-11-03 Hanall Pharmaceuticals, Co., Ltd. Protease resistant modified interferon alpha polypeptides
US20050202438A1 (en) * 2002-09-09 2005-09-15 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
US20060020396A1 (en) * 2002-09-09 2006-01-26 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
CA2499816C (en) 2002-09-27 2013-07-30 Xencor, Inc. Optimized fc variants and methods for their generation
US8158768B2 (en) 2002-12-23 2012-04-17 Dynavax Technologies Corporation Immunostimulatory sequence oligonucleotides and methods of using the same
CA2515583C (en) 2003-03-07 2015-07-14 Diversa Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
SG155777A1 (en) 2003-04-09 2009-10-29 Neose Technologies Inc Glycopegylation methods and proteins/peptides produced by the methods
EP1644538A4 (en) * 2003-06-26 2006-11-08 Invitrogen Corp METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSION PROTEINS
WO2005049831A1 (en) * 2003-11-20 2005-06-02 Agency For Science Technology And Research Method
JP2007512838A (ja) 2003-12-01 2007-05-24 インヴィトロジェン コーポレーション 組換え部位を含む核酸分子およびその使用方法
US7204950B2 (en) * 2003-12-19 2007-04-17 Pepsico, Inc. Dispensing package
ES2552053T3 (es) 2004-02-25 2015-11-25 Pioneer Hi-Bred International Inc. Nuevos polipéptidos cristalinos de Bacillus thuringiensis, polinucleótidos, y composiciones de los mismos
ES2379368T3 (es) 2004-05-21 2012-04-25 The Regents Of The University Of California Método para aumentar la producción de compuestos isoprenoides
BRPI0511868A (pt) 2004-06-09 2008-01-15 Pioneer Hi Bred Internacional peptìdeo isolado, polipeptìdeo de fusão, moléculas de ácido nucléico isoladas, vetores, métodos de direcionamento de polipeptìdeos e método de identificação de peptìdeos
AU2005330514B2 (en) 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
CA2590245A1 (en) * 2004-11-11 2006-05-18 Modular Genetics, Inc. Ladder assembly and system for generating diversity
EP1869174B1 (en) 2005-03-10 2015-02-18 BASF Enzymes LLC Lyase enzymes, nucleic acids encoding them and methods for making and using them
CA2611859C (en) 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
US20070184472A1 (en) * 2005-06-08 2007-08-09 Toagosei Co., Ltd Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
CN101228188A (zh) 2005-06-21 2008-07-23 佐马技术有限公司 IL-1β结合抗体及其片段
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
EP2444487B1 (en) 2006-02-10 2018-04-18 BP Corporation North America Inc. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
CA2946924A1 (en) 2006-02-14 2007-08-23 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
ES2416104T3 (es) 2006-03-07 2013-07-30 Cargill, Incorporated Aldolasas, ácidos nucleicos que las codifican y métodos para producir y usar las mismas
CN102690833B (zh) 2006-03-07 2015-09-09 巴斯夫酶有限责任公司 醛缩酶、编码它们的核酸及制备和使用它们的方法
WO2007134327A2 (en) 2006-05-15 2007-11-22 Sea Lane Biotechnologies, Llc. Neutralizing antibodies to influenza viruses
WO2007146975A2 (en) * 2006-06-13 2007-12-21 Athenix Corporation Methods for generating genetic diversity by permutational mutagenesis
BRPI0714876B1 (pt) 2006-08-04 2022-04-19 Verenium Corporation Ácido nucleico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula bacteriana, fúngica ou de levedura transformada, polipeptídeo isolado, sintético ou recombinante, composição, bem como métodos de produção e de usos dos mesmos
EP2617823B1 (en) 2006-09-21 2015-07-01 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
PL2057274T3 (pl) 2006-09-21 2014-05-30 Dsm Ip Assets Bv Fosfolipazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i stosowania
CA2832111A1 (en) 2006-12-20 2008-07-03 Mmrglobal, Inc. Antibodies and methods for making and using them
WO2008080093A2 (en) 2006-12-21 2008-07-03 Verenium Corporation Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them
CA2674721C (en) 2007-01-30 2018-04-03 Verenium Corporation Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
EP3431099B1 (en) 2007-03-30 2024-01-03 The Research Foundation for The State University of New York Attenuated viruses useful for vaccines
BRPI0815046A2 (pt) 2007-07-31 2014-10-14 Verenium Corp Personalização de montagem combinatória de múltiplos locais
CN101952437B (zh) 2007-10-03 2014-04-09 维莱尼姆公司 木聚糖酶、编码它们的核酸以及其制备和应用方法
MX2010005693A (es) 2007-12-03 2010-06-01 Syngenta Participations Ag Proteinas suceptibles diseñadas enzimaticamente.
EP2980217A1 (en) * 2007-12-31 2016-02-03 XOMA Technology Ltd. Methods and materials for targeted mutagenesis
WO2009088949A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
US8541220B2 (en) 2008-01-03 2013-09-24 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
CN102159241A (zh) * 2008-01-28 2011-08-17 塔皮穆内公司 用于增强免疫应答的泰帕森(Tapasin)增加
EP2698380A1 (en) 2008-03-28 2014-02-19 Sea Lane Biotechnologies, LLC Neutralizing molecules to viral antigens
DK2297333T3 (en) * 2008-05-30 2015-04-07 Massachusetts Inst Technology Method for spatial separation and for screening cells
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
ES2667773T3 (es) * 2008-12-02 2018-05-14 Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet) Protozoo modificado que expresa al menos dos proteínas variables de superficie (VSP), vacuna que lo comprende, procedimientos, usos y métodos
US20120190071A1 (en) 2009-05-12 2012-07-26 Council Of Scientific & Industrial Research Lipa and its variant useful for biofuel production
AU2010249500B2 (en) 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
CN102625848A (zh) 2009-07-17 2012-08-01 生物蛋白有限公司 在生产宿主中同时整合抗体/蛋白的性能和表达的筛选和演化的方法
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
WO2011109726A2 (en) * 2010-03-05 2011-09-09 Bioatla Llc Homologous multi-specific antibodies
ES2966088T3 (es) 2010-07-16 2024-04-18 Bioatla Inc Nuevos métodos de evolución de proteínas
AU2011289283B2 (en) 2010-08-13 2015-04-30 Pioneer Hi-Bred International, Inc. Chimeric promoters and methods of use
JP2014502144A (ja) 2010-10-06 2014-01-30 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド バリアントcbhiポリペプチド
WO2012099566A1 (en) 2010-11-17 2012-07-26 Sea Lane Biotechnologies, Llc Influenza virus neutralizing agents that mimic the binding site of an influenza neutralizing antibody
US20140182011A1 (en) 2011-11-03 2014-06-26 The University Of Hong Kong Methods Using Acyl-Coenzyme A-Binding Proteins to Enchance Drought Tolerance in Genetically Modified Plants
EP2838999A1 (en) 2012-03-16 2015-02-25 BP Corporation North America Inc. Polypeptides having endoglucanase activity
CN104411823A (zh) 2012-05-04 2015-03-11 纳幕尔杜邦公司 包含具有大范围核酸酶活性的序列的组合物和方法
AU2013259276B2 (en) 2012-05-10 2018-03-22 Bioatla Llc Multi-specific monoclonal antibodies
JP6616687B2 (ja) 2012-08-16 2019-12-04 バングラデシュ ジュート リサーチ インスティテュート Macrophominaphaseolina由来のセルロースおよび/またはヘミセルロース分解酵素およびその使用
JP6644547B2 (ja) 2012-08-16 2020-02-12 バングラデシュ ジュート リサーチ インスティテュートBangladesh Jute Research Institute Macrophominaphaseolina由来のペクチン分解酵素およびその使用
US10253325B2 (en) 2012-12-19 2019-04-09 Boston Medical Center Corporation Methods for elevating fat/oil content in plants
SG11201505969XA (en) * 2013-01-31 2015-08-28 Codexis Inc Methods, systems, and software for identifying bio-molecules with interacting components
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
US20140289906A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
ES2819865T3 (es) 2014-01-07 2021-04-19 Bioatla Llc Proteínas dirigidas a ortólogos
US20180291413A1 (en) 2015-10-06 2018-10-11 Thermo Fisher Scientific Geneart Gmbh Devices and methods for producing nucleic acids and proteins
WO2017075456A1 (en) 2015-10-30 2017-05-04 Beth Israel Deaconess Medical Center, Inc. Methods for determination of bioactivity, quantity, removal, or inactivation of cereal amylase trypsin inhibitors in cereals, flours, plants, and complex foods
CN111148528A (zh) 2016-12-28 2020-05-12 英福瓦克思公司 流感疫苗
MX2020004318A (es) 2017-10-25 2020-08-13 Basf Se Enzimas beta-amilasa.
CA3083527A1 (en) * 2017-12-20 2019-06-27 Basf Se Gene site saturation mutagenesis (gssm) method
BR112020021692A2 (pt) 2018-04-26 2021-01-26 Basf Se polipeptídeo tendo atividade de lipase, polipeptídeo híbrido, composição, polinucleotídeo variante, método para fabricar a variante de polipeptídeo, e, uso do polipeptídeo
WO2019211143A1 (en) 2018-05-03 2019-11-07 Basf Se Amylase enzymes
US12351841B2 (en) 2018-05-29 2025-07-08 Basf Se Amylase enzymes
KR20210009421A (ko) 2018-06-14 2021-01-26 바이오아트라, 엘엘씨 다중 특이적 항체 구조체
CN113412332A (zh) 2018-09-07 2021-09-17 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
CN112969792A (zh) 2018-09-07 2021-06-15 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
US20220025391A1 (en) 2018-09-07 2022-01-27 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
CN114096271B (zh) 2019-04-23 2024-04-12 巴斯夫欧洲公司 β-淀粉酶变体
MX2022007714A (es) 2019-12-20 2022-07-19 Basf Se Disminucion de la toxicidad de terpenos y aumento de la posible produccion en microorganismos.
BR112022024475A2 (pt) 2020-06-04 2022-12-27 Isobionics B V Beta santaleno sintase sintética, ácido nucleico sintético, cassete de expressão, método para produzir uma composição, célula hospedeira não humana, composição, e, uso de qualquer uma das santaleno sintases sintéticas
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
US20240352490A1 (en) 2021-08-02 2024-10-24 Basf Se Novel production of aroma compounds with ionylideneethane synthases

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959312A (en) 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP1997891A1 (en) 1988-09-02 2008-12-03 Dyax Corporation Generation and selection of recombinant varied binding proteins
US5198346A (en) 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US5096815A (en) 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
US5677149A (en) 1992-11-24 1997-10-14 G.D. Searle & Co., Interleukin-3 (IL-3) mutant polypeptides and their recombinant production
BR9407767A (pt) * 1993-10-08 1997-03-18 Novo Nordisk As Variante de enzima &-amilase uso da mesma construção de DNA vetor de express o recombinante célula processos para produzir uma &-amilase hibrida e para preparar uma variante de uma &-amilase aditivo detergente e composições detergentes
US6187579B1 (en) 1993-10-28 2001-02-13 Carlsberg A/S Customized proteases
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US5389537A (en) 1994-01-21 1995-02-14 Wisconsin Alumni Research Foundation Nuclease having altered specificity
WO1996006188A1 (en) 1994-08-18 1996-02-29 Cytogen Corporation Peptide librairies as a source of syngenes
AU3719395A (en) 1994-09-21 1996-04-09 Cytogen Corporation Antigen binding peptides (abtides) from peptide libraries
US5885577A (en) 1994-09-21 1999-03-23 Cytogen Corporation Antigen binding peptides (abtides) from peptide libraries
CA2219080A1 (en) 1995-06-07 1996-12-27 Ariad Gene Therapeutics, Inc. Rapamycin-based regulation of biological events
US5789166A (en) 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
US5885827A (en) 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
ES2236634T3 (es) 1997-04-07 2005-07-16 Genentech, Inc. Anticuerpos anti-vegf.
TR199902878T2 (xx) 1997-04-18 2000-02-21 Biogen,Inc. Tip II TGF-Beta Resept�r/�m�noglob�lin sabit b�l�m f�zyon proteinleri.
WO1998049286A2 (en) 1997-05-01 1998-11-05 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
EP1032683B1 (en) * 1997-11-17 2005-02-02 Novozymes Biotech, Inc. Polypeptides having 5-aminolevulinic acid synthase activity and nucleic acids encoding same
EP1042457B1 (en) * 1997-12-16 2006-03-08 Novozymes A/S Polypeptides having aminopeptidase activity and nucleic acids encoding same
AU755784B2 (en) 1998-01-15 2002-12-19 Ariad Pharmaceuticals, Inc. Regulation of biological events using multimeric chimeric proteins

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