JP2024160269A5 - - Google Patents

Download PDF

Info

Publication number
JP2024160269A5
JP2024160269A5 JP2024124506A JP2024124506A JP2024160269A5 JP 2024160269 A5 JP2024160269 A5 JP 2024160269A5 JP 2024124506 A JP2024124506 A JP 2024124506A JP 2024124506 A JP2024124506 A JP 2024124506A JP 2024160269 A5 JP2024160269 A5 JP 2024160269A5
Authority
JP
Japan
Prior art keywords
nucleic acid
target nucleic
sequence
barcode
fragments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2024124506A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024160269A (ja
Filing date
Publication date
Priority claimed from JP2020204004A external-priority patent/JP7127104B2/ja
Application filed filed Critical
Publication of JP2024160269A publication Critical patent/JP2024160269A/ja
Publication of JP2024160269A5 publication Critical patent/JP2024160269A5/ja
Pending legal-status Critical Current

Links

JP2024124506A 2014-10-17 2024-07-31 連続性を維持した転位 Pending JP2024160269A (ja)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201462065544P 2014-10-17 2014-10-17
US62/065,544 2014-10-17
US201562157396P 2015-05-05 2015-05-05
US62/157,396 2015-05-05
US201562242880P 2015-10-16 2015-10-16
US62/242,880 2015-10-16
JP2020204004A JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2022129859A Division JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位

Publications (2)

Publication Number Publication Date
JP2024160269A JP2024160269A (ja) 2024-11-13
JP2024160269A5 true JP2024160269A5 (enExample) 2024-11-21

Family

ID=55747561

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017520884A Active JP6808617B2 (ja) 2014-10-17 2015-10-16 連続性を維持した転位
JP2020204004A Active JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A Active JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位
JP2024124506A Pending JP2024160269A (ja) 2014-10-17 2024-07-31 連続性を維持した転位

Family Applications Before (3)

Application Number Title Priority Date Filing Date
JP2017520884A Active JP6808617B2 (ja) 2014-10-17 2015-10-16 連続性を維持した転位
JP2020204004A Active JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A Active JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位

Country Status (10)

Country Link
US (3) US11873480B2 (enExample)
JP (4) JP6808617B2 (enExample)
KR (2) KR102643955B1 (enExample)
AU (2) AU2015331739B2 (enExample)
BR (2) BR122021026781B1 (enExample)
CA (1) CA2964799A1 (enExample)
IL (3) IL299976B2 (enExample)
RU (2) RU2736728C2 (enExample)
SG (2) SG11201703139VA (enExample)
WO (1) WO2016061517A2 (enExample)

Families Citing this family (180)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
DK2954065T3 (da) 2013-02-08 2021-09-06 10X Genomics Inc Opdeling og bearbejdning af analytter og andre arter
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
KR102531677B1 (ko) 2014-06-26 2023-05-10 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
KR20170073667A (ko) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. 표적화 핵산 서열 분석을 위한 방법 및 조성물
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
KR102321863B1 (ko) 2015-01-12 2021-11-08 10엑스 제노믹스, 인크. 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리
SG11201706730XA (en) 2015-02-17 2017-09-28 Dovetail Genomics Llc Nucleic acid sequence assembly
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
EP4603581A3 (en) * 2015-05-28 2025-11-12 Illumina Cambridge Limited Surface-based tagmentation
WO2017004612A1 (en) 2015-07-02 2017-01-05 Arima Genomics, Inc. Accurate molecular deconvolution of mixtures samples
US11479805B2 (en) 2015-08-21 2022-10-25 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
EP3365445B1 (en) * 2015-10-19 2023-05-31 Dovetail Genomics, LLC Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
US10730030B2 (en) 2016-01-08 2020-08-04 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
EP3402883A4 (en) * 2016-01-12 2019-09-18 Seqwell, Inc. COMPOSITIONS AND METHODS FOR THE SEQUENCING OF NUCLEIC ACIDS
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
SG11201807117WA (en) 2016-02-23 2018-09-27 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
US20170283864A1 (en) 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
CN119574873A (zh) 2016-05-02 2025-03-07 Encodia公司 采用核酸编码的大分子分析
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
JP7497976B2 (ja) 2016-05-13 2024-06-11 ダブテイル ゲノミクス エルエルシー 保存されたサンプルからの長距離連鎖情報の回復
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
WO2018013724A1 (en) * 2016-07-12 2018-01-18 Kapa Biosystems, Inc. System and method for transposase-mediated amplicon sequencing
ES2873723T3 (es) * 2016-07-22 2021-11-03 Univ Oregon Health & Science Colecciones de genoma completo de células individuales y métodos de indexación combinatoria para prepararlas
WO2018031760A1 (en) * 2016-08-10 2018-02-15 Grail, Inc. Methods of preparing dual-indexed dna libraries for bisulfite conversion sequencing
JP2019528059A (ja) * 2016-08-10 2019-10-10 プレジデント アンド フェローズ オブ ハーバード カレッジ バーコード化ゲノムdna断片のデノボアセンブリの方法
WO2018057779A1 (en) * 2016-09-23 2018-03-29 Jianbiao Zheng Compositions of synthetic transposons and methods of use thereof
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11021738B2 (en) 2016-12-19 2021-06-01 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented DNA
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622219D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Methods and reagents for molecular barcoding
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10968447B2 (en) * 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3583112A4 (en) * 2017-02-14 2021-04-07 Seqwell, Inc. COMPOSITIONS AND METHODS FOR THE SEQUENCING OF NUCLEIC ACIDS
IL263118B2 (en) * 2017-02-21 2023-12-01 Illumina Inc Tegumentation using fixed transpososomes with linkers
JP7197567B2 (ja) 2017-05-05 2022-12-27 スキピオ バイオサイエンス ハイドロゲル中の個別の生物学的単位を捕捉およびバーコード付与するための方法
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
DK3981884T3 (da) * 2017-06-07 2023-09-04 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker til methyleringssekventering
WO2019060722A2 (en) * 2017-09-22 2019-03-28 X Gen Us Co. METHODS AND COMPOSITIONS FOR USE IN PREPARING POLYNUCLEOTIDES
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019076768A1 (en) * 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP4241882B1 (en) 2017-10-27 2025-04-30 10X Genomics, Inc. Methods for sample preparation and analysis
US11513126B2 (en) 2017-10-31 2022-11-29 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN111655848A (zh) 2017-11-21 2020-09-11 阿瑞玛基因组学公司 在核酸模板中保留空间邻位邻接性和分子邻接性
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
EP3728631A1 (en) 2017-12-22 2020-10-28 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
AU2019214956B2 (en) 2018-01-31 2025-06-26 Dovetail Genomics, Llc Sample prep for DNA linkage recovery
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
JP7511344B2 (ja) 2018-04-20 2024-07-05 イルミナ インコーポレイテッド 単一細胞を封入する方法、封入された細胞およびその使用
US12152271B2 (en) 2018-04-27 2024-11-26 Cygnus Biosciences (Beijing) Co., Ltd Methods and compositions for preparing polynucleotides
WO2019213294A1 (en) * 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
EP4245861B1 (en) * 2018-05-08 2025-02-26 MGI Tech Co., Ltd. Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
CN119955902A (zh) 2018-05-17 2025-05-09 伊鲁米纳公司 具有减少的扩增偏倚的高通量单细胞测序
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
EP4249651B1 (en) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2020061903A1 (zh) * 2018-09-27 2020-04-02 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
US11667957B2 (en) * 2018-10-25 2023-06-06 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
EP3894552B1 (en) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
IL281664B2 (en) 2019-01-11 2024-07-01 Illumina Cambridge Ltd Complex surface-bound transposome complexes
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
WO2020157684A1 (en) 2019-01-29 2020-08-06 Mgi Tech Co., Ltd. High coverage stlfr
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
TWI873119B (zh) 2019-01-29 2025-02-21 美商伊路米納有限公司 流通槽及製備其之方法
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US20220127597A1 (en) 2019-02-14 2022-04-28 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized tn5 transposase
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CN114072499B (zh) 2019-04-30 2024-08-06 Encodia公司 用于制备分析物的方法和相关试剂盒
CN113226519B (zh) 2019-07-12 2024-10-01 Illumina剑桥有限公司 使用电泳制备核酸文库
CA3125241A1 (en) * 2019-07-12 2021-01-21 Illumina Cambridge Limited Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7558249B2 (ja) * 2019-08-19 2024-09-30 ユニバーサル シーケンシング テクノロジー コーポレイション 核酸シーケンシングの核酸フラグメント起源を追跡するための方法および組成物
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
MX2021015885A (es) * 2019-09-20 2022-03-22 Illumina Inc Métodos y composiciones para identificar ligandos en arreglos usando índices y códigos de barra.
US12553044B2 (en) 2019-10-25 2026-02-17 Changping National Laboratory Methylation detection and analysis of mammalian DNA
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
JP2023504946A (ja) * 2019-12-02 2023-02-08 イルミナ・ケンブリッジ・リミテッド ゲノムdnaの増幅された連続性保存ライブラリーフラグメントの時間ベースのクラスター撮像
EP3927824A2 (en) 2019-12-19 2021-12-29 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
EP4090763B1 (en) 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
US20230081062A1 (en) * 2020-02-12 2023-03-16 Universal Sequencing Technology Corporation Methods for intracellular barcoding and spatial barcoding
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
WO2021247593A1 (en) 2020-06-02 2021-12-09 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
WO2022010965A1 (en) * 2020-07-08 2022-01-13 Illumina, Inc. Beads as transposome carriers
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116438319A (zh) * 2020-10-21 2023-07-14 Illumina公司 包含多个插入物的测序模板以及用于提高测序通量的组合物和方法
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
WO2022125997A1 (en) * 2020-12-11 2022-06-16 The Broad Institute, Inc. Method for duplex sequencing
CN114622286A (zh) * 2020-12-14 2022-06-14 中国农业大学 一种高通量的转录组测序文库构建方法及其应用
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CA3208854A1 (en) * 2021-02-04 2022-08-11 Illumina, Inc. Long indexed-linked read generation on transposome bound beads
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN112950101B (zh) * 2021-05-13 2021-08-17 北京富通东方科技有限公司 一种基于产量预测的汽车工厂混流线排产方法
EP4430206A1 (en) 2021-11-10 2024-09-18 Encodia, Inc. Methods for barcoding macromolecules in individual cells
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
CA3257292A1 (en) * 2022-05-26 2023-11-30 Illumina, Inc. PREPARATION OF LONG-READING NUCLEIC ACID LIBRARIES
US20250305047A1 (en) * 2022-06-09 2025-10-02 The Regents Of The University Of California Single cell co-sequencing of dna methylation and rna
KR20250092173A (ko) 2022-10-27 2025-06-23 스미또모 가가꾸 가부시끼가이샤 올리고뉴클레오티드의 제조 방법
WO2024137703A1 (en) * 2022-12-20 2024-06-27 Illumina, Inc. Multivalent assemblies for enhanced target hybridization
WO2025072677A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Tagmentation methods and kits

Family Cites Families (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
JP2801051B2 (ja) 1988-06-24 1998-09-21 アムジエン・インコーポレーテツド 核酸塩基配列を検出するための方法及び試薬
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
ATE137269T1 (de) 1990-01-26 1996-05-15 Abbott Lab Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
EP0754240B1 (en) 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
KR100230718B1 (ko) 1994-03-16 1999-11-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 등온 가닥 변위 핵산 증폭법
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
KR100551104B1 (ko) 1994-12-09 2006-02-09 임페리얼 컬리지 이노베이션스 리미티드 유전자의 동정
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
FI103809B1 (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20010046669A1 (en) 1999-02-24 2001-11-29 Mccobmie William R. Genetically filtered shotgun sequencing of complex eukaryotic genomes
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20050191698A1 (en) 1999-04-20 2005-09-01 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001034790A1 (en) * 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method for synthesizing nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP1290225A4 (en) 2000-05-20 2004-09-15 Univ Michigan METHOD FOR PRODUCING A DNA BANK BY POSITIONAL REPRODUCTION
US20030064366A1 (en) 2000-07-07 2003-04-03 Susan Hardin Real-time sequence determination
US6846658B1 (en) 2000-10-12 2005-01-25 New England Biolabs, Inc. Method for cloning and producing the Msel restriction endonuclease
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20040110191A1 (en) 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US6777187B2 (en) 2001-05-02 2004-08-17 Rubicon Genomics, Inc. Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
GB0115194D0 (en) 2001-06-21 2001-08-15 Leuven K U Res & Dev Novel technology for genetic mapping
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
AU2003277984A1 (en) 2002-11-05 2004-06-07 The University Of Queensland Nucleotide sequence analysis by quantification of mutagenesis
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
ATE497540T1 (de) 2003-02-10 2011-02-15 Max Delbrueck Centrum Transposon-system zur gezielten integration
CA2528577C (en) 2003-06-20 2012-08-07 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP2202322A1 (en) 2003-10-31 2010-06-30 AB Advanced Genetic Analysis Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
WO2005100585A2 (en) 2004-03-30 2005-10-27 Epicentre Methods for obtaining directionally truncated polypeptides
US20080268507A1 (en) 2004-05-25 2008-10-30 Airbus Deutschland Gmbh Recombinant Dna Nicking Endonuclease and Uses Thereof
US7319142B1 (en) 2004-08-31 2008-01-15 Monsanto Technology Llc Nucleotide and amino acid sequences from Xenorhabdus and uses thereof
EP1790202A4 (en) 2004-09-17 2013-02-20 Pacific Biosciences California APPARATUS AND METHOD FOR ANALYZING MOLECULES
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
US7601499B2 (en) 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070128610A1 (en) 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20070172839A1 (en) 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
EP2495337A1 (en) 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US20100022403A1 (en) 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20090088331A1 (en) 2006-11-07 2009-04-02 Government Of The United States Of America, As Represented By The Secretary, Dept. Of Health And Influenza virus nucleic acid microarray and method of use
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2639578B1 (en) 2006-12-14 2016-09-14 Life Technologies Corporation Apparatus for measuring analytes using large scale fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
DK2258871T3 (da) 2007-01-19 2014-08-11 Epigenomics Ag Fremgangsmåder og nukleinsyrer til analyse af celleproliferative lidelser
BRPI0813718A2 (pt) 2007-07-13 2014-12-30 Univ Leland Stanford Junior Método e aparelho que utilizam um campo elétrico para ensaios biológicos aperfeiçoados
WO2009024019A1 (en) 2007-08-15 2009-02-26 The University Of Hong Kong Methods and compositions for high-throughput bisulphite dna-sequencing and utilities
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
WO2009092035A2 (en) 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
AU2009267086B2 (en) 2008-06-30 2016-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
DK2787565T3 (en) * 2008-10-24 2015-10-12 Epict Technologies Corp Transposon-end COMPOSITIONS AND METHODS TO MODIFY nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
SG174155A1 (en) 2009-02-25 2011-10-28 Univ Johns Hopkins Piggybac transposon variants and methods of use
WO2010115122A2 (en) 2009-04-03 2010-10-07 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
WO2011106314A2 (en) 2010-02-25 2011-09-01 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
WO2012027572A2 (en) 2010-08-27 2012-03-01 Genentech, Inc. Methods for nucleic acid capture and sequencing
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
CN103228798B (zh) 2010-09-24 2015-12-09 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
WO2012048341A1 (en) * 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CA2821299C (en) * 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
ES2762866T3 (es) 2011-01-28 2020-05-26 Illumina Inc Reemplazo de nucleótidos por bibliotecas doblemente etiquetadas y direccionales
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
NO2694769T3 (enExample) * 2012-03-06 2018-03-03
EP2825672B1 (en) 2012-03-13 2019-02-13 Swift Biosciences, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
DK2954065T3 (da) * 2013-02-08 2021-09-06 10X Genomics Inc Opdeling og bearbejdning af analytter og andre arter
US10550426B2 (en) * 2013-03-07 2020-02-04 Sekisui Medical Co., Ltd. Method for detecting methylated DNA
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
EP4617376A3 (en) 2013-05-23 2025-12-10 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
CN106460033B (zh) 2013-12-30 2021-12-24 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition

Similar Documents

Publication Publication Date Title
JP2024160269A5 (enExample)
AU2022201205B2 (en) Contiguity Preserving Transposition
US20220333160A1 (en) Methods for selectively suppressing non-target sequences
US8986958B2 (en) Methods for generating target specific probes for solution based capture
AU2012212148B2 (en) Massively parallel contiguity mapping
EP3192869B1 (en) Isolated oligonucleotide and use thereof in nucleic acid sequencing
CN107969137B (zh) 接近性保留性转座
JP3042626B2 (ja) オリゴヌクレオチドバンクを用いるdna塩基配列の決定法
US20210155985A1 (en) Surface concatemerization of templates
CN110139931B (zh) 用于定相测序的方法和组合物
US20120015821A1 (en) Methods of Generating Gene Specific Libraries
WO2010030683A1 (en) Methods of generating gene specific libraries
WO2013117595A2 (en) Targeted enrichment and amplification of nucleic acids on a support
JP2004524012A (ja) 固相支持体上における核酸の等温増幅
EP2242855A1 (en) Paired end sequencing
US20150267256A1 (en) Method for the simultaneous amplification of a plurality of different nucleic acid target sequences
JP2006506953A5 (enExample)
US20230348961A1 (en) Ex-situ sequencing of rca product generated in-situ
EP4455307A1 (en) Ex-situ sequencing of rca product generated in-situ
RU2020137454A (ru) Транспозиция с сохранением сцепления генов
HK40068143A (en) Contiguity preserving transposition
CN119563034A (zh) 用于增强靶杂交的多价组装体