JP2006506953A5 - - Google Patents
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- Publication number
- JP2006506953A5 JP2006506953A5 JP2003587952A JP2003587952A JP2006506953A5 JP 2006506953 A5 JP2006506953 A5 JP 2006506953A5 JP 2003587952 A JP2003587952 A JP 2003587952A JP 2003587952 A JP2003587952 A JP 2003587952A JP 2006506953 A5 JP2006506953 A5 JP 2006506953A5
- Authority
- JP
- Japan
- Prior art keywords
- signature
- tag
- adapter
- cdna
- library
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012634 fragment Substances 0.000 claims description 17
- 108091033319 polynucleotide Proteins 0.000 claims description 7
- 102000040430 polynucleotide Human genes 0.000 claims description 7
- 239000002157 polynucleotide Substances 0.000 claims description 7
- 230000000295 complement effect Effects 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 4
- 239000007790 solid phase Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims 25
- 239000002299 complementary DNA Substances 0.000 claims 15
- 108091008146 restriction endonucleases Proteins 0.000 claims 10
- 238000003776 cleavage reaction Methods 0.000 claims 9
- 230000007017 scission Effects 0.000 claims 9
- 108091034117 Oligonucleotide Proteins 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 238000010367 cloning Methods 0.000 claims 2
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- 101150101095 Mmp12 gene Proteins 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 238000012408 PCR amplification Methods 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 239000007791 liquid phase Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 238000010839 reverse transcription Methods 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37578202P | 2002-04-26 | 2002-04-26 | |
| PCT/US2003/013076 WO2003091416A2 (en) | 2002-04-26 | 2003-04-25 | Constant length signatures for parallel sequencing of polynucleotides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2006506953A JP2006506953A (ja) | 2006-03-02 |
| JP2006506953A5 true JP2006506953A5 (enExample) | 2010-01-21 |
| JP4446746B2 JP4446746B2 (ja) | 2010-04-07 |
Family
ID=29270698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003587952A Expired - Fee Related JP4446746B2 (ja) | 2002-04-26 | 2003-04-25 | ポリヌクレオチドの並行配列決定のための一定長シグネチャー |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20040002104A1 (enExample) |
| EP (1) | EP1497465B1 (enExample) |
| JP (1) | JP4446746B2 (enExample) |
| AT (1) | ATE423224T1 (enExample) |
| AU (1) | AU2003234255A1 (enExample) |
| CA (1) | CA2482425A1 (enExample) |
| DE (1) | DE60326224D1 (enExample) |
| WO (1) | WO2003091416A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1523554A2 (en) * | 2002-06-12 | 2005-04-20 | Riken | Method of utilizing the 5' end of transcribed nucleic acid regions for cloning and analysis |
| ES2725808T3 (es) | 2003-05-23 | 2019-09-27 | Nektar Therapeutics | Derivados de PEG que contienen dos cadenas de PEG |
| US7947261B2 (en) | 2003-05-23 | 2011-05-24 | Nektar Therapeutics | Conjugates formed from polymer derivatives having particular atom arrangements |
| WO2005079357A2 (en) * | 2004-02-17 | 2005-09-01 | Dana Farber Cancer Institute | Nucleic acid representations utilizing type iib restriction endonuclease cleavage products |
| EP1582599A1 (en) * | 2004-03-31 | 2005-10-05 | Takara Bio Inc. | Method for purifying microbeads |
| WO2006003721A1 (en) * | 2004-07-02 | 2006-01-12 | Kabushiki Kaisha Dnaform | Method for preparing sequence tags |
| EP2171097A2 (en) | 2007-06-29 | 2010-04-07 | Population Genetics Technologies LTD. | Methods and compositions for isolating nucleic acid sequence variants |
| US10781481B2 (en) | 2016-04-27 | 2020-09-22 | Bio-Rad Laboratories, Inc. | miRNA transcriptome methods and compositions |
| WO2018015365A1 (en) * | 2016-07-18 | 2018-01-25 | Roche Sequencing Solutions, Inc. | Asymmetric templates and asymmetric method of nucleic acid sequencing |
| CN108410856A (zh) * | 2018-03-29 | 2018-08-17 | 武汉光谷创赢生物技术开发有限公司 | 一种全长cDNA合成方法及其测序文库的构建 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5093245A (en) * | 1988-01-26 | 1992-03-03 | Applied Biosystems | Labeling by simultaneous ligation and restriction |
| US6107023A (en) * | 1988-06-17 | 2000-08-22 | Genelabs Technologies, Inc. | DNA amplification and subtraction techniques |
| CA2036946C (en) * | 1990-04-06 | 2001-10-16 | Kenneth V. Deugau | Indexing linkers |
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| US5604097A (en) * | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US6013445A (en) * | 1996-06-06 | 2000-01-11 | Lynx Therapeutics, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
| US5629179A (en) * | 1995-03-17 | 1997-05-13 | Novagen, Inc. | Method and kit for making CDNA library |
| US5695937A (en) * | 1995-09-12 | 1997-12-09 | The Johns Hopkins University School Of Medicine | Method for serial analysis of gene expression |
| US5866330A (en) * | 1995-09-12 | 1999-02-02 | The Johns Hopkins University School Of Medicine | Method for serial analysis of gene expression |
| US6461814B1 (en) * | 1997-01-15 | 2002-10-08 | Dominic G. Spinella | Method of identifying gene transcription patterns |
| US6232067B1 (en) * | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| JP2003527087A (ja) * | 1999-08-13 | 2003-09-16 | イェール・ユニバーシティ | バイナリーコード化配列タグ |
| US6274320B1 (en) * | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
-
2003
- 2003-04-25 DE DE60326224T patent/DE60326224D1/de not_active Expired - Lifetime
- 2003-04-25 AT AT03728567T patent/ATE423224T1/de not_active IP Right Cessation
- 2003-04-25 WO PCT/US2003/013076 patent/WO2003091416A2/en not_active Ceased
- 2003-04-25 AU AU2003234255A patent/AU2003234255A1/en not_active Abandoned
- 2003-04-25 JP JP2003587952A patent/JP4446746B2/ja not_active Expired - Fee Related
- 2003-04-25 EP EP03728567A patent/EP1497465B1/en not_active Expired - Lifetime
- 2003-04-25 CA CA002482425A patent/CA2482425A1/en not_active Abandoned
- 2003-04-25 US US10/424,281 patent/US20040002104A1/en not_active Abandoned
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