DE60326224D1 - Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden - Google Patents
Signaturen konstanter länge für das parallele sequenzieren von polynukleotidenInfo
- Publication number
- DE60326224D1 DE60326224D1 DE60326224T DE60326224T DE60326224D1 DE 60326224 D1 DE60326224 D1 DE 60326224D1 DE 60326224 T DE60326224 T DE 60326224T DE 60326224 T DE60326224 T DE 60326224T DE 60326224 D1 DE60326224 D1 DE 60326224D1
- Authority
- DE
- Germany
- Prior art keywords
- parallel sequencing
- signatures
- polynucleotides
- constant length
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012163 sequencing technique Methods 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37578202P | 2002-04-26 | 2002-04-26 | |
| PCT/US2003/013076 WO2003091416A2 (en) | 2002-04-26 | 2003-04-25 | Constant length signatures for parallel sequencing of polynucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60326224D1 true DE60326224D1 (de) | 2009-04-02 |
Family
ID=29270698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60326224T Expired - Lifetime DE60326224D1 (de) | 2002-04-26 | 2003-04-25 | Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20040002104A1 (enExample) |
| EP (1) | EP1497465B1 (enExample) |
| JP (1) | JP4446746B2 (enExample) |
| AT (1) | ATE423224T1 (enExample) |
| AU (1) | AU2003234255A1 (enExample) |
| CA (1) | CA2482425A1 (enExample) |
| DE (1) | DE60326224D1 (enExample) |
| WO (1) | WO2003091416A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1523554A2 (en) * | 2002-06-12 | 2005-04-20 | Riken | Method of utilizing the 5' end of transcribed nucleic acid regions for cloning and analysis |
| ES2725808T3 (es) | 2003-05-23 | 2019-09-27 | Nektar Therapeutics | Derivados de PEG que contienen dos cadenas de PEG |
| US7947261B2 (en) | 2003-05-23 | 2011-05-24 | Nektar Therapeutics | Conjugates formed from polymer derivatives having particular atom arrangements |
| WO2005079357A2 (en) * | 2004-02-17 | 2005-09-01 | Dana Farber Cancer Institute | Nucleic acid representations utilizing type iib restriction endonuclease cleavage products |
| EP1582599A1 (en) * | 2004-03-31 | 2005-10-05 | Takara Bio Inc. | Method for purifying microbeads |
| WO2006003721A1 (en) * | 2004-07-02 | 2006-01-12 | Kabushiki Kaisha Dnaform | Method for preparing sequence tags |
| EP2171097A2 (en) | 2007-06-29 | 2010-04-07 | Population Genetics Technologies LTD. | Methods and compositions for isolating nucleic acid sequence variants |
| US10781481B2 (en) | 2016-04-27 | 2020-09-22 | Bio-Rad Laboratories, Inc. | miRNA transcriptome methods and compositions |
| WO2018015365A1 (en) * | 2016-07-18 | 2018-01-25 | Roche Sequencing Solutions, Inc. | Asymmetric templates and asymmetric method of nucleic acid sequencing |
| CN108410856A (zh) * | 2018-03-29 | 2018-08-17 | 武汉光谷创赢生物技术开发有限公司 | 一种全长cDNA合成方法及其测序文库的构建 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5093245A (en) * | 1988-01-26 | 1992-03-03 | Applied Biosystems | Labeling by simultaneous ligation and restriction |
| US6107023A (en) * | 1988-06-17 | 2000-08-22 | Genelabs Technologies, Inc. | DNA amplification and subtraction techniques |
| CA2036946C (en) * | 1990-04-06 | 2001-10-16 | Kenneth V. Deugau | Indexing linkers |
| US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
| US5604097A (en) * | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US6013445A (en) * | 1996-06-06 | 2000-01-11 | Lynx Therapeutics, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
| US5629179A (en) * | 1995-03-17 | 1997-05-13 | Novagen, Inc. | Method and kit for making CDNA library |
| US5695937A (en) * | 1995-09-12 | 1997-12-09 | The Johns Hopkins University School Of Medicine | Method for serial analysis of gene expression |
| US5866330A (en) * | 1995-09-12 | 1999-02-02 | The Johns Hopkins University School Of Medicine | Method for serial analysis of gene expression |
| US6461814B1 (en) * | 1997-01-15 | 2002-10-08 | Dominic G. Spinella | Method of identifying gene transcription patterns |
| US6232067B1 (en) * | 1998-08-17 | 2001-05-15 | The Perkin-Elmer Corporation | Adapter directed expression analysis |
| JP2003527087A (ja) * | 1999-08-13 | 2003-09-16 | イェール・ユニバーシティ | バイナリーコード化配列タグ |
| US6274320B1 (en) * | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
-
2003
- 2003-04-25 DE DE60326224T patent/DE60326224D1/de not_active Expired - Lifetime
- 2003-04-25 AT AT03728567T patent/ATE423224T1/de not_active IP Right Cessation
- 2003-04-25 WO PCT/US2003/013076 patent/WO2003091416A2/en not_active Ceased
- 2003-04-25 AU AU2003234255A patent/AU2003234255A1/en not_active Abandoned
- 2003-04-25 JP JP2003587952A patent/JP4446746B2/ja not_active Expired - Fee Related
- 2003-04-25 EP EP03728567A patent/EP1497465B1/en not_active Expired - Lifetime
- 2003-04-25 CA CA002482425A patent/CA2482425A1/en not_active Abandoned
- 2003-04-25 US US10/424,281 patent/US20040002104A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1497465A2 (en) | 2005-01-19 |
| WO2003091416A3 (en) | 2004-07-01 |
| JP4446746B2 (ja) | 2010-04-07 |
| ATE423224T1 (de) | 2009-03-15 |
| JP2006506953A (ja) | 2006-03-02 |
| AU2003234255A1 (en) | 2003-11-10 |
| EP1497465B1 (en) | 2009-02-18 |
| WO2003091416A2 (en) | 2003-11-06 |
| US20040002104A1 (en) | 2004-01-01 |
| EP1497465A4 (en) | 2005-06-08 |
| CA2482425A1 (en) | 2003-11-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |