JP2017514478A5 - - Google Patents
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- Publication number
- JP2017514478A5 JP2017514478A5 JP2016564984A JP2016564984A JP2017514478A5 JP 2017514478 A5 JP2017514478 A5 JP 2017514478A5 JP 2016564984 A JP2016564984 A JP 2016564984A JP 2016564984 A JP2016564984 A JP 2016564984A JP 2017514478 A5 JP2017514478 A5 JP 2017514478A5
- Authority
- JP
- Japan
- Prior art keywords
- forming
- cdna
- rna
- acid sequence
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920002477 rna polymer Polymers 0.000 claims 38
- 239000002299 complementary DNA Substances 0.000 claims 31
- 108020004635 Complementary DNA Proteins 0.000 claims 29
- 238000010804 cDNA synthesis Methods 0.000 claims 29
- 150000007523 nucleic acids Chemical group 0.000 claims 22
- 238000000034 method Methods 0.000 claims 21
- 108091033319 polynucleotide Proteins 0.000 claims 19
- 102000040430 polynucleotide Human genes 0.000 claims 19
- 239000002157 polynucleotide Substances 0.000 claims 19
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 16
- 108020004414 DNA Proteins 0.000 claims 15
- 108091034117 Oligonucleotide Proteins 0.000 claims 11
- 239000007787 solid Substances 0.000 claims 10
- 239000003795 chemical substances by application Substances 0.000 claims 7
- 230000003100 immobilizing effect Effects 0.000 claims 7
- 238000012408 PCR amplification Methods 0.000 claims 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 2
- 238000010839 reverse transcription Methods 0.000 claims 2
- 239000011324 bead Substances 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461990598P | 2014-05-08 | 2014-05-08 | |
| US61/990,598 | 2014-05-08 | ||
| US201462079495P | 2014-11-13 | 2014-11-13 | |
| US62/079,495 | 2014-11-13 | ||
| PCT/US2015/029986 WO2015172080A1 (en) | 2014-05-08 | 2015-05-08 | Integrated single cell sequencing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017514478A JP2017514478A (ja) | 2017-06-08 |
| JP2017514478A5 true JP2017514478A5 (enExample) | 2018-06-07 |
| JP6716465B2 JP6716465B2 (ja) | 2020-07-01 |
Family
ID=54393068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016564984A Active JP6716465B2 (ja) | 2014-05-08 | 2015-05-08 | 集積した単一細胞の配列決定 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20150337295A1 (enExample) |
| EP (1) | EP3140428B1 (enExample) |
| JP (1) | JP6716465B2 (enExample) |
| CN (1) | CN106460051A (enExample) |
| SG (2) | SG11201609055RA (enExample) |
| WO (1) | WO2015172080A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106811460B (zh) | 2015-11-30 | 2020-11-27 | 浙江安诺优达生物科技有限公司 | 用于低频突变检测的二代测序文库的构建方法及试剂盒 |
| JPWO2017204294A1 (ja) * | 2016-05-25 | 2019-02-21 | 株式会社ニコン | 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置 |
| CA3095762A1 (en) * | 2018-04-04 | 2019-10-10 | Nautilus Biotechnology, Inc. | Methods of generating nanoarrays and microarrays |
| US10842821B2 (en) | 2018-04-05 | 2020-11-24 | Medeor Therapeutics, Inc. | Cellular compositions derived from prior organ donors and methods of manufacture and use thereof |
| US10881692B2 (en) | 2018-04-05 | 2021-01-05 | Medeor Therapeutics, Inc. | Compositions for establishing mixed chimerism and methods of manufacture thereof |
| SG11202102722SA (en) * | 2018-10-25 | 2021-04-29 | Illumina Inc | Methods and compositions for identifying ligands on arrays using indexes and barcodes |
| EP3917660A4 (en) * | 2019-01-29 | 2022-11-23 | Molecular Assemblies, Inc. | REUSABLE INITIATORS FOR NUCLEIC ACID SYNTHESIS |
| EP4441240A4 (en) * | 2021-12-02 | 2025-11-12 | Bio Rad Laboratories Inc | USE OF DNA/RNA DUPLEX FRAGMENTATION |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9620769D0 (en) * | 1996-10-04 | 1996-11-20 | Brax Genomics Ltd | Nucleic acid sequencing |
| EP1591541B1 (en) * | 1997-04-01 | 2012-02-15 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| GB9714716D0 (en) | 1997-07-11 | 1997-09-17 | Brax Genomics Ltd | Characterising nucleic acids |
| US20060275782A1 (en) * | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| EP2308982B1 (en) * | 2000-04-17 | 2014-11-26 | Dyax Corp. | Methods of constructing display libraries of genetic packages for members of a diverse family of peptides |
| AUPQ974900A0 (en) * | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
| EP1353935A4 (en) * | 2000-12-07 | 2005-02-23 | Penn State Res Found | SELECTION OF TARGET CATALYTIC NUCLEIC ACIDS ON INFECTIOUS AGENTS |
| JP2004097158A (ja) * | 2002-09-12 | 2004-04-02 | Kureha Chem Ind Co Ltd | 発現遺伝子同定用ｃＤＮＡタグの作成方法、及び該ｃＤＮＡタグを用いる遺伝子発現解析方法 |
| WO2007123744A2 (en) * | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
| US20110129827A1 (en) | 2008-04-04 | 2011-06-02 | Helicos Biosciences Corporation | Methods for transcript analysis |
| US20100035249A1 (en) * | 2008-08-05 | 2010-02-11 | Kabushiki Kaisha Dnaform | Rna sequencing and analysis using solid support |
| US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| US9625454B2 (en) * | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| CN118086471A (zh) | 2010-12-17 | 2024-05-28 | 生命技术公司 | 用于核酸扩增的方法、组合物、系统、仪器和试剂盒 |
| WO2012162267A2 (en) * | 2011-05-20 | 2012-11-29 | Fluidigm Corporation | Nucleic acid encoding reactions |
| US9429500B2 (en) | 2012-02-29 | 2016-08-30 | Fluidigm Corporation | Methods, systems and devices for multiple single-cell capturing and processing using microfluidics |
| NO2694769T3 (enExample) * | 2012-03-06 | 2018-03-03 | ||
| CN105164246B (zh) | 2013-03-15 | 2018-09-25 | 富鲁达公司 | 用于分析定义的多细胞组合的方法和设备 |
-
2015
- 2015-05-08 SG SG11201609055RA patent/SG11201609055RA/en unknown
- 2015-05-08 US US14/708,048 patent/US20150337295A1/en not_active Abandoned
- 2015-05-08 WO PCT/US2015/029986 patent/WO2015172080A1/en not_active Ceased
- 2015-05-08 SG SG10202004286UA patent/SG10202004286UA/en unknown
- 2015-05-08 EP EP15789313.2A patent/EP3140428B1/en active Active
- 2015-05-08 CN CN201580024133.5A patent/CN106460051A/zh active Pending
- 2015-05-08 JP JP2016564984A patent/JP6716465B2/ja active Active
-
2018
- 2018-12-21 US US16/229,786 patent/US20190249238A1/en not_active Abandoned