JP2016518843A5 - - Google Patents

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JP2016518843A5
JP2016518843A5 JP2016512029A JP2016512029A JP2016518843A5 JP 2016518843 A5 JP2016518843 A5 JP 2016518843A5 JP 2016512029 A JP2016512029 A JP 2016512029A JP 2016512029 A JP2016512029 A JP 2016512029A JP 2016518843 A5 JP2016518843 A5 JP 2016518843A5
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oligonucleotide
oligonucleotides
labeled
detectable moiety
different
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JP2016518843A (ja
JP6605452B2 (ja
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JP2016512029A 2013-04-30 2014-04-30 逐次ハイブリダイゼーションバーコーディングによる分子の多重標識化 Active JP6605452B2 (ja)

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US201361817651P 2013-04-30 2013-04-30
US61/817,651 2013-04-30
US201461971974P 2014-03-28 2014-03-28
US61/971,974 2014-03-28
PCT/US2014/036258 WO2014182528A2 (en) 2013-04-30 2014-04-30 Multiplex labeling of molecules by sequential hybridization barcoding

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JP2016518843A JP2016518843A (ja) 2016-06-30
JP2016518843A5 true JP2016518843A5 (enExample) 2017-06-15
JP6605452B2 JP6605452B2 (ja) 2019-11-13

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JP2019189650A Pending JP2020028295A (ja) 2013-04-30 2019-10-16 逐次ハイブリダイゼーションバーコーディングによる分子の多重標識化
JP2022074240A Pending JP2022115923A (ja) 2013-04-30 2022-04-28 逐次ハイブリダイゼーションバーコーディングによる分子の多重標識化

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JP2022074240A Pending JP2022115923A (ja) 2013-04-30 2022-04-28 逐次ハイブリダイゼーションバーコーディングによる分子の多重標識化

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US (5) US10457980B2 (enExample)
EP (2) EP3626833A1 (enExample)
JP (3) JP6605452B2 (enExample)
KR (2) KR102701095B1 (enExample)
CN (2) CN110669826B (enExample)
CA (1) CA2907493A1 (enExample)
DK (1) DK2992115T4 (enExample)
FI (1) FI2992115T4 (enExample)
WO (1) WO2014182528A2 (enExample)

Families Citing this family (145)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011319755B2 (en) 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
EP3604555B1 (en) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
EP4491741B1 (en) 2011-12-22 2025-10-15 President And Fellows Of Harvard College Compositions and methods for analyte detection
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
AU2013300171B2 (en) 2012-08-09 2017-09-14 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for preparing biological specimens for microscopic analysis
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
DK2920725T3 (da) 2012-11-19 2022-02-07 Apton Biosystems Inc Digital analyse af molekylære analytter ved hjælp af enkeltmolekyledetektering
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
CN110669826B (zh) * 2013-04-30 2025-01-07 加州理工学院 通过顺序杂交编条形码的分子多重标记
SG10201710030QA (en) 2013-06-04 2018-01-30 Harvard College Rna-guided transcriptional regulation
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
KR20170013855A (ko) 2014-05-30 2017-02-07 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 큰 손상되지 않은 조직 샘플들을 이미징하기 위한 방법들 및 디바이스들
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
CN107075545A (zh) 2014-07-30 2017-08-18 哈佛学院院长及董事 探针文库构建
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
US20160194701A1 (en) * 2014-11-21 2016-07-07 Nanostring Technologies, Inc. Enzyme- and amplification-free sequencing
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
EP4368992A3 (en) 2015-07-17 2024-08-07 Bruker Spatial Biology, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
WO2017079406A1 (en) 2015-11-03 2017-05-11 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
WO2017139501A1 (en) 2016-02-10 2017-08-17 The Board Of Trustees Of The Leland Stanford Junior University Rna fixation and detection in clarity-based hydrogel tissue
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US11512344B2 (en) 2016-05-10 2022-11-29 Arizona Board Of Regents On Behalf Of Arizona State University Consecutive hybridization for multiplexed analysis of biological samples
US11269171B2 (en) * 2016-05-19 2022-03-08 Huron Technologies International Inc. Spectrally-resolved scanning microscope
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
US10370698B2 (en) * 2016-07-27 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
EP3491151B1 (en) 2016-08-01 2026-03-11 California Institute of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
US12421540B2 (en) 2016-08-01 2025-09-23 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光in situ配列決定による検出のための核酸配列ライブラリの作製法
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
EP3299472A1 (en) * 2016-09-27 2018-03-28 Deutsches Krebsforschungszentrum Stiftung des Öffentlichen Rechts Method for labeling oligonucleotide probes
EP3519612B1 (en) * 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
US11352660B2 (en) * 2016-10-04 2022-06-07 Howard Hughes Medical Institute Materials and methods for serial multiplexed detection of RNA in cells and tissues
KR102476709B1 (ko) 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
EP3551765B1 (en) * 2016-12-09 2026-02-04 Ultivue, Inc. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
US11492661B2 (en) * 2017-01-10 2022-11-08 President And Fellows Of Harvard College Multiplexed signal amplification
CN110650794B (zh) 2017-03-17 2022-04-29 雅普顿生物系统公司 测序和高分辨率成像
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
WO2018218150A1 (en) 2017-05-26 2018-11-29 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
WO2019055852A1 (en) * 2017-09-15 2019-03-21 Apton Biosystems, Inc. PROFILING OF HETEROGENEOUS UNICELLULAR ORGANISMS USING MOLECULAR BARCODE CODING
EP4741512A2 (en) 2017-09-22 2026-05-13 University of Washington In situ combinatorial labeling of cellular molecules
CA3078158A1 (en) 2017-10-06 2019-04-11 Cartana Ab Rna templated ligation
US11753676B2 (en) 2017-10-11 2023-09-12 Expansion Technologies Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy
WO2019103996A1 (en) 2017-11-21 2019-05-31 Expansion Technologies Expansion microscopy compatible and multiplexed in situ hybridization of formalin fixed paraffin embedded tissue sections for spatially resolved transcriptomics
CA3083224A1 (en) * 2017-12-08 2019-06-13 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridzation of probes
EP3743525A4 (en) 2018-01-26 2021-09-29 President and Fellows of Harvard College PROXIMITY DETECTION METHODS AND COMPOSITIONS
KR102879375B1 (ko) * 2018-02-12 2025-10-30 브루커 스페이셜 바이올로지, 인크. 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법
JP7525402B2 (ja) 2018-05-14 2024-07-30 ナノストリング テクノロジーズ,インコーポレイティド 化学的組成物とそれを利用する方法
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
KR20260023102A (ko) 2018-09-19 2026-02-20 퍼시픽 바이오사이언스 오브 캘리포니아, 인크. 밀집 패킹된 분석물층 및 검출 방법
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
JP2022506518A (ja) * 2018-11-01 2022-01-17 カリフォルニア インスティチュート オブ テクノロジー 高速広視野共焦点イメージング用のマルチチャネルラインスキャナ
US11421273B2 (en) 2018-12-03 2022-08-23 California Institute Of Technology In situ readout of DNA barcodes
CA3122569A1 (en) 2018-12-13 2020-06-18 President And Fellows Of Harvard College Amplification methods and systems for merfish and other applications
US11674178B2 (en) 2018-12-14 2023-06-13 Ultivue, Inc. Methods and compositions for sequentially detecting targets
US11965208B2 (en) * 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
SG11202111878RA (en) 2019-05-31 2021-11-29 10X Genomics Inc Method of detecting target nucleic acid molecules
CN110484440A (zh) * 2019-08-30 2019-11-22 希格诺(南京)生物科技有限公司 一种核酸荧光原位检测装置及方法
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
CN115066501A (zh) * 2019-12-19 2022-09-16 阿科亚生物科学股份有限公司 Rna检测
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
CN115362264A (zh) 2020-01-29 2022-11-18 10X基因组学有限公司 用于分析物检测的组合物和方法
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
CN115917007A (zh) * 2020-02-18 2023-04-04 新加坡科技研究局 核酸探针
EP4484571A3 (en) 2020-02-21 2025-03-19 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
JP2023529682A (ja) 2020-06-18 2023-07-11 リゾルブ バイオサイエンシズ ゲゼルシャフト ミット ベシュレンクテル ハフツング 試料中の異なる分析物を検出するための多重的方法
US20210395804A1 (en) * 2020-06-19 2021-12-23 California Institute Of Technology Sensitive and multiplexed detection of nucleic acids and proteins for large scale serological testing
US20220042097A1 (en) * 2020-08-04 2022-02-10 The Broad Institute, Inc. In-situ spatial transcriptomics and proteomics
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
EP4214712A2 (en) 2020-09-16 2023-07-26 10X Genomics, Inc. Methods and systems for barcode error correction
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
AU2021385065A1 (en) 2020-11-18 2023-06-15 10X Genomics, Inc. Methods and compositions for analyzing immune infiltration in cancer stroma to predict clinical outcome
US11333588B1 (en) 2020-12-07 2022-05-17 Nebulum Technologies Co., Ltd. Matrix-assisted methods and compositions to prepare biological samples for super-resolution imaging
US12509717B2 (en) 2020-12-11 2025-12-30 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム, インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4301873A1 (en) 2021-03-03 2024-01-10 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022251711A1 (en) 2021-05-28 2022-12-01 California Institute Of Technology Massive generation of chemically ligateable probes for multiplexed fish
EP4347877A1 (en) 2021-06-01 2024-04-10 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
EP4347880B1 (en) 2021-06-02 2026-02-04 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2022261255A1 (en) 2021-06-08 2022-12-15 California Institute Of Technology Ratiometric symbols and sequential coding for multiplexed fish
US20230026886A1 (en) 2021-07-13 2023-01-26 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
KR20240046190A (ko) 2021-08-06 2024-04-08 리졸브 바이오사이언시즈 게엠베하 병원체 포함 샘플에서 분석물을 검출하는 방법
WO2023023484A1 (en) 2021-08-16 2023-02-23 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
KR20240097850A (ko) 2021-10-25 2024-06-27 리졸브 바이오사이언시즈 게엠베하 표면에 생물학적 시료를 장착하는 방법
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023122345A1 (en) * 2021-12-23 2023-06-29 California Institute Of Technology Suppression of non-specific signals by exonucleases in fish experiment
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
WO2023141588A1 (en) 2022-01-21 2023-07-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
EP4504969A1 (en) 2022-04-06 2025-02-12 10X Genomics, Inc. Methods for multiplex cell analysis
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
WO2023220300A1 (en) 2022-05-11 2023-11-16 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024002726A1 (en) 2022-06-30 2024-01-04 Resolve Biosciences Gmbh High resolution multiplex method for detecting at least two targets with a distance of beyond the diffraction limit in a sample
CN119301155A (zh) 2022-07-19 2025-01-10 百进生物科技公司 抗cd157抗体、其抗原结合片段和组合物及其制备和使用方法
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
US12116626B2 (en) 2022-08-16 2024-10-15 10X Genomics, Inc. AP50 polymerases and uses thereof
WO2024040114A2 (en) 2022-08-18 2024-02-22 BioLegend, Inc. Anti-axl antibodies, antigen-binding fragments thereof and methods for making and using the same
CN120129825A (zh) 2022-10-14 2025-06-10 10X基因组学有限公司 用于评估生物样品质量的方法、组合物和系统
WO2024102736A1 (en) 2022-11-08 2024-05-16 10X Genomics, Inc. Immobilization methods and compositions for in situ detection
WO2024107727A1 (en) 2022-11-18 2024-05-23 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4622746A1 (en) 2022-11-22 2025-10-01 10X Genomics, Inc. Systems and methods for interfacing with a bottle
US12581050B2 (en) 2022-11-23 2026-03-17 10X Genomics, Inc. Optical assemblies for machine vision calibration
DE102022131444A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131451A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131445A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131450A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131449A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131446A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131447A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131441A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131442A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Zuordnen von Bildbereichen einer Bildfolge zu Ergebnisklassen mittels eines Analytdaten-Auswertesystems mit einem Verarbeitungsmodells
DE102022131448A1 (de) 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
WO2024167929A1 (en) * 2023-02-06 2024-08-15 Applied Materials, Inc. Target probes with landmark sequences for localizing gene transcripts by fluorescence in situ hybridization
CN121219425A (zh) * 2023-04-11 2025-12-26 哈佛学院院长及董事 化学连接技术
EP4720123A1 (en) 2023-05-25 2026-04-08 Biolegend, Inc. Ceacam6 binding antibodies and antigen-binding fragments thereof
CN121866343A (zh) * 2023-06-05 2026-04-14 哈佛学院院长及董事 用于测定厚组织中的核酸的三维成像系统及方法
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
DE102023122213A1 (de) 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen
CN121816366A (zh) 2023-09-27 2026-04-07 百进公司 抗gpc4抗体
WO2025093690A1 (en) 2023-11-02 2025-05-08 Resolve Biosciences Gmbh Method for preparing tissue sections
WO2025099313A1 (en) 2023-11-10 2025-05-15 Resolve Biosciences Gmbh Reducing background fluorescence in single molecule fluorescence in situ hybridization applications
WO2025125442A1 (en) 2023-12-13 2025-06-19 Resolve Biosciences Gmbh High resolution multiplex method for detecting at least two targets
WO2025132546A1 (en) 2023-12-19 2025-06-26 Resolve Biosciences Gmbh Method for reducing nonspecific signals in in-situ hybridization assays
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
DE102024116989A1 (de) * 2024-06-17 2025-12-18 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen eines Konfidenzmaßes beim Auswerten von Analyt-Bilddaten einer Probe

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1228811A (en) 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US6280929B1 (en) 1986-01-16 2001-08-28 The Regents Of The University Of California Method of detecting genetic translocations identified with chromosomal abnormalities
CA1317860C (en) 1987-04-01 1993-05-18 Daniel Louis Kacian Techniques for preparing specimens for bacterial assays
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5629147A (en) * 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
US5955272A (en) * 1993-02-26 1999-09-21 University Of Massachusetts Detection of individual gene transcription and splicing
US20030017081A1 (en) * 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5866331A (en) * 1995-10-20 1999-02-02 University Of Massachusetts Single molecule detection by in situ hybridization
US6194146B1 (en) * 1997-06-13 2001-02-27 Bioseparations, Inc. Situ and in vitro hybridization method and buffer
ATE329264T1 (de) 1998-03-18 2006-06-15 Roche Diagnostics Gmbh Nachweis von produkten von apoptosis
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
JP2003527075A (ja) 1999-03-25 2003-09-16 ハイセック,インコーポレーテッド ハイブリダイゼーションによる配列分析のための、溶液ベースの方法
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP1171636A2 (en) 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Assay of gene expression patterns by multi-fluor fish
JP2003535600A (ja) 2000-06-02 2003-12-02 バイエル コーポレイション インサイチュ分枝dnaハイブリダイゼーションを用いた高感度遺伝子検出および位置決定
CA2451614C (en) * 2001-06-25 2011-01-04 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes
US7189509B2 (en) 2001-08-16 2007-03-13 Zhifeng Shao Analysis of gene expression profiles using sequential hybridization
CA2459320C (en) * 2001-09-06 2016-07-05 Genomic Profiling Systems, Inc. Rapid detection of replicating cells
AU2002341898A2 (en) * 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
JP2005521410A (ja) 2002-03-29 2005-07-21 ジェネンテック・インコーポレーテッド 核酸分析試料の検出と定量化のための方法及び組成物
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー/アクセプター部分が相補鎖上となる標的核酸検出のmet/fretに基づく方法
US20040171075A1 (en) * 2002-12-31 2004-09-02 Flynn Daniel L Modulation of protein functionalities
WO2004074447A2 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US7727721B2 (en) * 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
WO2007001986A2 (en) 2005-06-20 2007-01-04 Yuling Luo Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US20090081688A1 (en) 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
ATE501277T1 (de) 2005-07-01 2011-03-15 Dako Denmark As Monomere und polymere linker zur konjugierung von biologischen molekülen und anderen stoffen
US20080269068A1 (en) * 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
ES2684317T3 (es) * 2008-08-14 2018-10-02 Nanostring Technologies, Inc Nanoinformadores estables
EP2356259A4 (en) 2008-10-30 2012-11-14 Sequenom Inc PRODUCTS AND METHOD FOR MULTIPLEX IDENTIFICATION OF NUCLEIC ACIDS
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
US20100221708A1 (en) * 2009-03-02 2010-09-02 Yamada N Alice Method for chromosome enumeration
US20100304994A1 (en) * 2009-06-02 2010-12-02 President And Fellows Of Havard College Oligonucleotide Paints
CN102459592B (zh) * 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
US9677125B2 (en) 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
US8965076B2 (en) 2010-01-13 2015-02-24 Illumina, Inc. Data processing system and methods
ES2606012T3 (es) 2010-02-26 2017-03-17 Ventana Medical Systems, Inc. Hibridación in situ con sondas PolyTag
WO2011112634A2 (en) * 2010-03-08 2011-09-15 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
US10266876B2 (en) * 2010-03-08 2019-04-23 California Institute Of Technology Multiplex detection of molecular species in cells by super-resolution imaging and combinatorial labeling
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
JP2013531801A (ja) * 2010-07-02 2013-08-08 ヴェンタナ メディカル システムズ, インク. 標的検出のためのハプテンコンジュゲート
US8962241B2 (en) * 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
CN106434871B (zh) 2011-05-17 2020-08-18 德克斯特里蒂诊断公司 用于检测目标核酸的方法与组合物
EP4491741B1 (en) 2011-12-22 2025-10-15 President And Fellows Of Harvard College Compositions and methods for analyte detection
US20140073520A1 (en) * 2011-12-23 2014-03-13 California Institute Of Technology Imaging chromosome structures by super-resolution fish with single-dye labeled oligonucleotides
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
US20140242581A1 (en) * 2013-01-23 2014-08-28 Reproductive Genetics And Technology Solutions, Llc Compositions and methods for genetic analysis of embryos
CN110669826B (zh) 2013-04-30 2025-01-07 加州理工学院 通过顺序杂交编条形码的分子多重标记
US20160369329A1 (en) * 2013-04-30 2016-12-22 California Institute Of Technology Multiplex labeling of molecules by sequential hybridization barcoding using probes with cleavable linkers
US20150225801A1 (en) 2014-02-11 2015-08-13 California Institute Of Technology Recording and mapping lineage information and molecular events in individual cells
US20180142307A1 (en) 2014-02-11 2018-05-24 California Institute Of Technology Recording and mapping lineage information and molecular events in individual cells
WO2016011429A1 (en) 2014-07-17 2016-01-21 California Institute Of Technology Multiplex analysis of molecules in single cells by image correlation
CN107075545A (zh) 2014-07-30 2017-08-18 哈佛学院院长及董事 探针文库构建
US12421540B2 (en) 2016-08-01 2025-09-23 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism

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