JP6716465B2 - 集積した単一細胞の配列決定 - Google Patents

集積した単一細胞の配列決定 Download PDF

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JP6716465B2
JP6716465B2 JP2016564984A JP2016564984A JP6716465B2 JP 6716465 B2 JP6716465 B2 JP 6716465B2 JP 2016564984 A JP2016564984 A JP 2016564984A JP 2016564984 A JP2016564984 A JP 2016564984A JP 6716465 B2 JP6716465 B2 JP 6716465B2
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nucleic acid
cdna
acid sequence
rna
immobilized
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JP2017514478A (ja
JP2017514478A5 (enExample
Inventor
エイ エイ ウェスト ジェイソン
エイ エイ ウェスト ジェイソン
ファウラー ブライアン
ファウラー ブライアン
チャーン ズーホウェ
チャーン ズーホウェ
エフ ジョンソン クリスチャン
エフ ジョンソン クリスチャン
エー アンガー マーク
エー アンガー マーク
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フリューダイム・コーポレイション
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2016564984A 2014-05-08 2015-05-08 集積した単一細胞の配列決定 Active JP6716465B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201461990598P 2014-05-08 2014-05-08
US61/990,598 2014-05-08
US201462079495P 2014-11-13 2014-11-13
US62/079,495 2014-11-13
PCT/US2015/029986 WO2015172080A1 (en) 2014-05-08 2015-05-08 Integrated single cell sequencing

Publications (3)

Publication Number Publication Date
JP2017514478A JP2017514478A (ja) 2017-06-08
JP2017514478A5 JP2017514478A5 (enExample) 2018-06-07
JP6716465B2 true JP6716465B2 (ja) 2020-07-01

Family

ID=54393068

Family Applications (1)

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JP2016564984A Active JP6716465B2 (ja) 2014-05-08 2015-05-08 集積した単一細胞の配列決定

Country Status (6)

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US (2) US20150337295A1 (enExample)
EP (1) EP3140428B1 (enExample)
JP (1) JP6716465B2 (enExample)
CN (1) CN106460051A (enExample)
SG (2) SG10202004286UA (enExample)
WO (1) WO2015172080A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106811460B (zh) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
JPWO2017204294A1 (ja) * 2016-05-25 2019-02-21 株式会社ニコン 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
WO2019195633A1 (en) * 2018-04-04 2019-10-10 Ignite Biosciences, Inc. Methods of generating nanoarrays and microarrays
US10842821B2 (en) 2018-04-05 2020-11-24 Medeor Therapeutics, Inc. Cellular compositions derived from prior organ donors and methods of manufacture and use thereof
US10881692B2 (en) 2018-04-05 2021-01-05 Medeor Therapeutics, Inc. Compositions for establishing mixed chimerism and methods of manufacture thereof
WO2020086746A1 (en) 2018-10-25 2020-04-30 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
EP3917660A4 (en) * 2019-01-29 2022-11-23 Molecular Assemblies, Inc. REUSABLE INITIATORS FOR NUCLEIC ACID SYNTHESIS
CN118339306A (zh) * 2021-12-02 2024-07-12 生物辐射实验室股份有限公司 Dna:rna双链体断裂的用途

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
ATE364718T1 (de) * 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
GB9714716D0 (en) * 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
CA2406236C (en) * 2000-04-17 2013-02-19 Dyax Corp. Novel methods of constructing libraries of genetic packages that collectively display the members of a diverse family of peptides, polypeptides or proteins
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
WO2002046449A2 (en) * 2000-12-07 2002-06-13 The Penn State Research Foundation Selection of catalytic nucleic acids targeted to infectious agents
JP2004097158A (ja) * 2002-09-12 2004-04-02 Kureha Chem Ind Co Ltd 発現遺伝子同定用ｃＤＮＡタグの作成方法、及び該ｃＤＮＡタグを用いる遺伝子発現解析方法
SG170802A1 (en) * 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
WO2009124255A2 (en) 2008-04-04 2009-10-08 Helicos Biosciences Corporation Methods for transcript analysis
US20100035249A1 (en) * 2008-08-05 2010-02-11 Kabushiki Kaisha Dnaform Rna sequencing and analysis using solid support
US9309566B2 (en) * 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
CN110079588B (zh) 2010-12-17 2024-03-15 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
NO2694769T3 (enExample) 2012-03-06 2018-03-03
CN105164246B (zh) 2013-03-15 2018-09-25 富鲁达公司 用于分析定义的多细胞组合的方法和设备

Also Published As

Publication number Publication date
EP3140428A1 (en) 2017-03-15
EP3140428A4 (en) 2018-02-28
US20150337295A1 (en) 2015-11-26
US20190249238A1 (en) 2019-08-15
JP2017514478A (ja) 2017-06-08
SG11201609055RA (en) 2016-11-29
SG10202004286UA (en) 2020-06-29
EP3140428B1 (en) 2021-07-28
CN106460051A (zh) 2017-02-22
WO2015172080A1 (en) 2015-11-12

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