JP6808617B2 - 連続性を維持した転位 - Google Patents

連続性を維持した転位 Download PDF

Info

Publication number
JP6808617B2
JP6808617B2 JP2017520884A JP2017520884A JP6808617B2 JP 6808617 B2 JP6808617 B2 JP 6808617B2 JP 2017520884 A JP2017520884 A JP 2017520884A JP 2017520884 A JP2017520884 A JP 2017520884A JP 6808617 B2 JP6808617 B2 JP 6808617B2
Authority
JP
Japan
Prior art keywords
nucleic acid
target nucleic
sequence
bases
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2017520884A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018501776A (ja
Inventor
ジェイ スティーマーズ フランク
ジェイ スティーマーズ フランク
エル ガンダーソン ケヴィン
エル ガンダーソン ケヴィン
チャン ファン
チャン ファン
リチャード ベトリー ジェイソン
リチャード ベトリー ジェイソン
アントニー ゴームリー ニール
アントニー ゴームリー ニール
メウールマン ウーター
メウールマン ウーター
ウィアー ジャクリーン
ウィアー ジャクリーン
イオアノウ アヴグスタ
イオアノウ アヴグスタ
ジェンキンス ガレス
ジェンキンス ガレス
ジャクソン ロザモンド
ジャクソン ロザモンド
モレル ナタリー
モレル ナタリー
ケイ ポコロック ドミトリー
ケイ ポコロック ドミトリー
ジェイ ノルベルク スティーブン
ジェイ ノルベルク スティーブン
ホー モーリー
ホー モーリー
キア アミラーリ
キア アミラーリ
ガルィシン イゴール
ガルィシン イゴール
パントーハ リゴー
パントーハ リゴー
Original Assignee
イルミナ ケンブリッジ リミテッド
イルミナ ケンブリッジ リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イルミナ ケンブリッジ リミテッド, イルミナ ケンブリッジ リミテッド filed Critical イルミナ ケンブリッジ リミテッド
Publication of JP2018501776A publication Critical patent/JP2018501776A/ja
Application granted granted Critical
Publication of JP6808617B2 publication Critical patent/JP6808617B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Glass Compositions (AREA)
  • Inorganic Insulating Materials (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
JP2017520884A 2014-10-17 2015-10-16 連続性を維持した転位 Active JP6808617B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462065544P 2014-10-17 2014-10-17
US62/065,544 2014-10-17
US201562157396P 2015-05-05 2015-05-05
US62/157,396 2015-05-05
US201562242880P 2015-10-16 2015-10-16
US62/242,880 2015-10-16
PCT/US2015/056040 WO2016061517A2 (en) 2014-10-17 2015-10-16 Contiguity preserving transposition

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020204004A Division JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位

Publications (2)

Publication Number Publication Date
JP2018501776A JP2018501776A (ja) 2018-01-25
JP6808617B2 true JP6808617B2 (ja) 2021-01-06

Family

ID=55747561

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017520884A Active JP6808617B2 (ja) 2014-10-17 2015-10-16 連続性を維持した転位
JP2020204004A Active JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A Active JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位
JP2024124506A Pending JP2024160269A (ja) 2014-10-17 2024-07-31 連続性を維持した転位

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020204004A Active JP7127104B2 (ja) 2014-10-17 2020-12-09 連続性を維持した転位
JP2022129859A Active JP7532455B2 (ja) 2014-10-17 2022-08-17 連続性を維持した転位
JP2024124506A Pending JP2024160269A (ja) 2014-10-17 2024-07-31 連続性を維持した転位

Country Status (10)

Country Link
US (3) US11873480B2 (enExample)
JP (4) JP6808617B2 (enExample)
KR (2) KR102643955B1 (enExample)
AU (2) AU2015331739B2 (enExample)
BR (2) BR122021026781B1 (enExample)
CA (1) CA2964799A1 (enExample)
IL (3) IL299976B2 (enExample)
RU (2) RU2736728C2 (enExample)
SG (2) SG10201903408VA (enExample)
WO (1) WO2016061517A2 (enExample)

Families Citing this family (177)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
CA2898456C (en) 2013-03-13 2020-11-10 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
EP4219710A3 (en) 2014-08-01 2023-08-16 Dovetail Genomics, LLC Tagging nucleic acids for sequence assembly
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
AU2016220135B2 (en) 2015-02-17 2021-07-29 Dovetail Genomics Llc Nucleic acid sequence assembly
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
GB2554572B (en) * 2015-03-26 2021-06-23 Dovetail Genomics Llc Physical linkage preservation in DNA storage
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
WO2016189331A1 (en) * 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
CN107922959A (zh) 2015-07-02 2018-04-17 阿瑞玛基因组学公司 混合物样品的精确分子去卷积
WO2017034970A1 (en) * 2015-08-21 2017-03-02 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
CA3002740A1 (en) 2015-10-19 2017-04-27 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
CN108431223A (zh) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 每液滴分辨率下的多个珠
US20190169602A1 (en) * 2016-01-12 2019-06-06 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
JP7441003B2 (ja) 2016-02-23 2024-02-29 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリのためのフェージングされたリードセットの生成とハプロタイプフェージング
US20170283864A1 (en) 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
DK3452591T5 (da) 2016-05-02 2024-09-09 Encodia Inc Makromolekyleanalyse under anvendelse af nukleinsyrekodning
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
IL262946B2 (en) 2016-05-13 2023-03-01 Dovetail Genomics Llc Retrieving long-range grip information from preserved samples
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US20180016632A1 (en) * 2016-07-12 2018-01-18 Kapa Biosystems, Inc. System and method for transposase-mediated amplicon sequencing
ES2873723T3 (es) * 2016-07-22 2021-11-03 Univ Oregon Health & Science Colecciones de genoma completo de células individuales y métodos de indexación combinatoria para prepararlas
WO2018031760A1 (en) * 2016-08-10 2018-02-15 Grail, Inc. Methods of preparing dual-indexed dna libraries for bisulfite conversion sequencing
EP3497219A4 (en) * 2016-08-10 2020-08-19 President and Fellows of Harvard College NOVO ASSEMBLY PROCESSES FROM GENOMIC DNA BARCODE FRAGMENTS
WO2018057779A1 (en) * 2016-09-23 2018-03-29 Jianbiao Zheng Compositions of synthetic transposons and methods of use thereof
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11021738B2 (en) 2016-12-19 2021-06-01 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented DNA
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622219D0 (en) 2016-12-23 2017-02-08 Cs Genetics Ltd Methods and reagents for molecular barcoding
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
WO2018144216A1 (en) * 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US20200002746A1 (en) * 2017-02-14 2020-01-02 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
KR102607830B1 (ko) 2017-02-21 2023-12-01 일루미나, 인코포레이티드 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화
SG11201910195WA (en) 2017-05-05 2019-11-28 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
CN116064732A (zh) * 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
CN110997932B (zh) * 2017-06-07 2024-06-04 俄勒冈健康科学大学 用于甲基化测序的单细胞全基因组文库
WO2019060722A2 (en) 2017-09-22 2019-03-28 X Gen Us Co. METHODS AND COMPOSITIONS FOR USE IN PREPARING POLYNUCLEOTIDES
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019076768A1 (en) * 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
US11513126B2 (en) 2017-10-31 2022-11-29 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
WO2019089959A1 (en) 2017-11-02 2019-05-09 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11873481B2 (en) 2017-11-21 2024-01-16 Arima Genomics, Inc. Preserving spatial-proximal contiguity and molecular contiguity in nucleic acid templates
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
CA3090102A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
SG11201911961RA (en) 2018-04-20 2020-01-30 Illumina Inc Methods of encapsulating single cells, the encapsulated cells and uses thereof
CN113383083B (zh) 2018-04-27 2024-12-10 赛纳生物科技(北京)有限公司 用于制备多核苷酸的方法和组合物
WO2019213294A1 (en) * 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP4545647A3 (en) 2018-05-03 2025-07-09 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
ES2947437T3 (es) * 2018-05-08 2023-08-09 Mgi Tech Co Ltd Creación de Códigos de barra compartidos en el ADN, en un solo tubo con perlas, para la secuenciación, haplotipado y ensamblaje preciso y rentable
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
IL271454B2 (en) 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
CN113166807B (zh) 2018-08-20 2024-06-04 生物辐射实验室股份有限公司 通过分区中条码珠共定位生成核苷酸序列
CN112739829A (zh) * 2018-09-27 2021-04-30 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
MX2021003768A (es) * 2018-10-25 2021-05-27 Illumina Inc Metodos y composiciones para identificar ligandos en arreglos usando indices y codigos de barra.
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
JP7561120B2 (ja) * 2019-01-11 2024-10-03 イルミナ ケンブリッジ リミテッド 複合体が表面結合されたトランスポソーム複合体
ES2989967T3 (es) 2019-01-23 2024-11-28 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
TWI857001B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 定序套組
TWI857000B (zh) 2019-01-29 2024-10-01 美商伊路米納有限公司 流體槽以及將複合物引入至流體槽之方法
US20220195624A1 (en) * 2019-01-29 2022-06-23 Mgi Tech Co., Ltd. High coverage stlfr
TWI873119B (zh) 2019-01-29 2025-02-21 美商伊路米納有限公司 流通槽及製備其之方法
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
CN118979095A (zh) 2019-02-12 2024-11-19 10X基因组学有限公司 用于加工核酸分子的方法
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
CA3128098A1 (en) 2019-02-14 2020-08-20 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized tn5 transposase
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
JP7582676B2 (ja) 2019-04-30 2024-11-13 エンコディア， インコーポレイテッド 分析物を調製するための方法および関連キット
EP3997223A1 (en) * 2019-07-12 2022-05-18 Illumina Cambridge Limited Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
EP3997224A1 (en) 2019-07-12 2022-05-18 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
WO2021011803A1 (en) 2019-07-16 2021-01-21 Omniome, Inc. Synthetic nucleic acids having non-natural structures
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
CN114667353A (zh) * 2019-08-19 2022-06-24 通用测序技术公司 用于核酸测序用于追踪核酸片段起源的方法和组合物
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
AU2020351230A1 (en) * 2019-09-20 2022-01-20 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
EP4048808B1 (en) * 2019-10-25 2024-12-11 Changping National Laboratory Methylation detection and analysis of mammalian dna
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
MY209482A (en) * 2019-12-02 2025-07-11 Illumina Cambridge Ltd Time-based cluster imaging of amplified contiguity preserved library fragments of genomic dna
KR20220118295A (ko) 2019-12-19 2022-08-25 일루미나, 인코포레이티드 고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
JP2023512781A (ja) * 2020-02-12 2023-03-29 ユニバーサル シーケンシング テクノロジー コーポレイション 細胞内バーコード化および空間的バーコード化のための方法
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
AU2021306281A1 (en) * 2020-07-08 2023-01-05 Illumina, Inc. Beads as transposome carriers
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
JP2023547366A (ja) * 2020-10-21 2023-11-10 イルミナ インコーポレイテッド 複数のインサートを含む配列決定鋳型、並びに配列決定スループットを改善するための組成物及び方法
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN114622286A (zh) * 2020-12-14 2022-06-14 中国农业大学 一种高通量的转录组测序文库构建方法及其应用
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN112950101B (zh) * 2021-05-13 2021-08-17 北京富通东方科技有限公司 一种基于产量预测的汽车工厂混流线排产方法
US11753677B2 (en) 2021-11-10 2023-09-12 Encodia, Inc. Methods for barcoding macromolecules in individual cells
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
CA3257292A1 (en) * 2022-05-26 2023-11-30 Illumina, Inc. PREPARATION OF LONG-READING NUCLEIC ACID LIBRARIES
WO2023239907A1 (en) * 2022-06-09 2023-12-14 The Regents Of The University Of California Single cell co-sequencing of dna methylation and rna
EP4477659A4 (en) 2022-10-27 2025-07-30 Sumitomo Chemical Co METHOD FOR PRODUCING AN OLIGONUCLEOTIDE
EP4638787A1 (en) * 2022-12-20 2025-10-29 Illumina, Inc. Multivalent assemblies for enhanced target hybridization
WO2025072677A1 (en) * 2023-09-29 2025-04-03 Illumina, Inc. Tagmentation methods and kits

Family Cites Families (120)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
AU3539089A (en) 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
DE68927373T2 (de) 1988-06-24 1997-03-20 Amgen Inc., Thousand Oaks, Calif. Verfahren und mittel zum nachweis von nukleinsäuresequenzen
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE68926504T2 (de) 1988-07-20 1996-09-12 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
ATE247716T1 (de) 1994-02-07 2003-09-15 Beckman Coulter Inc Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
EP1285960A3 (en) 1994-12-09 2003-05-07 Imperial College Innovations Limited Virulence genes from Salmonella typhimurium
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US20020055100A1 (en) 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
FI103809B (fi) 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20010046669A1 (en) 1999-02-24 2001-11-29 Mccobmie William R. Genetically filtered shotgun sequencing of complex eukaryotic genomes
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
BR0015382B1 (pt) 1999-11-08 2014-04-29 Eiken Chemical Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2001274869A1 (en) 2000-05-20 2001-12-03 The Regents Of The University Of Michigan Method of producing a dna library using positional amplification
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
US6846658B1 (en) 2000-10-12 2005-01-25 New England Biolabs, Inc. Method for cloning and producing the Msel restriction endonuclease
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20040110191A1 (en) 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
US6777187B2 (en) 2001-05-02 2004-08-17 Rubicon Genomics, Inc. Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
GB0115194D0 (en) 2001-06-21 2001-08-15 Leuven K U Res & Dev Novel technology for genetic mapping
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
WO2004042078A1 (en) 2002-11-05 2004-05-21 The University Of Queensland Nucleotide sequence analysis by quantification of mutagenesis
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1594971B1 (en) 2003-02-10 2011-02-02 Max-Delbrück-Centrum für Molekulare Medizin (MDC) Transposon-based targeting system
JP2007525963A (ja) 2003-06-20 2007-09-13 イルミナ インコーポレイテッド 全ゲノム増幅および遺伝型決定のための方法および組成物
EP1682680B2 (en) 2003-10-31 2018-03-21 AB Advanced Genetic Analysis Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
WO2005100585A2 (en) 2004-03-30 2005-10-27 Epicentre Methods for obtaining directionally truncated polypeptides
WO2006047183A2 (en) 2004-10-21 2006-05-04 New England Biolabs, Inc. Recombinant dna nicking endonuclease and uses thereof
US7319142B1 (en) 2004-08-31 2008-01-15 Monsanto Technology Llc Nucleotide and amino acid sequences from Xenorhabdus and uses thereof
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
EP1910537A1 (en) 2005-06-06 2008-04-16 454 Life Sciences Corporation Paired end sequencing
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070128610A1 (en) 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
EP2038429B1 (en) 2006-06-30 2013-08-21 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20090088331A1 (en) 2006-11-07 2009-04-02 Government Of The United States Of America, As Represented By The Secretary, Dept. Of Health And Influenza virus nucleic acid microarray and method of use
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
ATE549418T1 (de) 2007-01-19 2012-03-15 Epigenomics Ag Verfahren und nukleinsäuren zur analyse proliferativer zellerkrankungen
AU2008276308A1 (en) 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
WO2009024019A1 (en) 2007-08-15 2009-02-26 The University Of Hong Kong Methods and compositions for high-throughput bisulphite dna-sequencing and utilities
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
EP3150727B1 (en) 2008-04-30 2019-07-10 Integrated DNA Technologies Inc. Rnase-h-based assays utilizing modified rna monomers
CA2729159C (en) 2008-06-30 2020-01-14 Bionanomatrix, Inc. Methods and devices for single-molecule whole genome analysis
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
CN105018548B (zh) * 2008-10-24 2019-03-12 阿霹震中科技公司 用于修饰核酸的试剂盒
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2401376A4 (en) 2009-02-25 2012-11-28 Univ Johns Hopkins TRANSPOSON PIGGYBAC VARIANTS AND METHODS OF USE
WO2010115122A2 (en) 2009-04-03 2010-10-07 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
HUE027972T2 (en) 2010-02-25 2016-11-28 Advanced Liquid Logic Inc A method for generating nucleic acid libraries
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
MX2013001799A (es) 2010-08-27 2013-05-20 Genentech Inc Metodos para la captura y secuenciacion de acido nucleico
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
AU2011323107B2 (en) * 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
DK3037536T3 (da) 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US9365897B2 (en) * 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
EP3395957B1 (en) 2011-04-25 2020-08-12 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
NO2694769T3 (enExample) * 2012-03-06 2018-03-03
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
CN104736722B (zh) 2012-05-21 2018-08-07 斯克利普斯研究所 样品制备方法
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
US10550426B2 (en) 2013-03-07 2020-02-04 Sekisui Medical Co., Ltd. Method for detecting methylated DNA
CA2898456C (en) * 2013-03-13 2020-11-10 Illumina, Inc. Methods and compositions for nucleic acid sequencing
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
SG11201605344YA (en) 2013-12-30 2016-07-28 Atreca Inc Analysis of nucleic acids associated with single cells using nucleic acid barcodes

Also Published As

Publication number Publication date
US20190040382A1 (en) 2019-02-07
WO2016061517A2 (en) 2016-04-21
SG10201903408VA (en) 2019-05-30
RU2019138705A (ru) 2020-01-27
IL287853A (en) 2022-01-01
US20220282242A1 (en) 2022-09-08
RU2017116989A3 (enExample) 2019-05-20
KR102643955B1 (ko) 2024-03-07
CA2964799A1 (en) 2016-04-21
JP7532455B2 (ja) 2024-08-13
IL251737B (en) 2021-12-01
RU2019138705A3 (enExample) 2020-05-22
RU2017116989A (ru) 2018-11-19
AU2015331739B2 (en) 2021-12-02
IL287853B2 (en) 2023-06-01
JP7127104B2 (ja) 2022-08-29
JP2018501776A (ja) 2018-01-25
BR112017007912A2 (pt) 2018-01-23
RU2736728C2 (ru) 2020-11-19
JP2022172158A (ja) 2022-11-15
IL251737A0 (en) 2017-06-29
AU2022201205B2 (en) 2024-12-05
BR122021026781B1 (pt) 2023-11-14
IL299976B2 (en) 2024-11-01
AU2022201205A1 (en) 2022-03-17
KR20170107423A (ko) 2017-09-25
KR20220162873A (ko) 2022-12-08
KR102472027B1 (ko) 2022-11-30
JP2024160269A (ja) 2024-11-13
IL299976B1 (en) 2024-07-01
JP2021052779A (ja) 2021-04-08
AU2015331739A1 (en) 2017-05-11
RU2709655C2 (ru) 2019-12-19
IL299976A (en) 2023-03-01
BR122021026779B1 (pt) 2023-12-19
US11873480B2 (en) 2024-01-16
SG11201703139VA (en) 2017-07-28
US20190048332A1 (en) 2019-02-14
WO2016061517A3 (en) 2016-06-23

Similar Documents

Publication Publication Date Title
JP7532455B2 (ja) 連続性を維持した転位
CN107969137B (zh) 接近性保留性转座
JP7638309B2 (ja) 低減した増幅バイアスによるハイスループット単一細胞シークエンシング
JP2020501554A (ja) 短いｄｎａ断片を連結することによる一分子シーケンスのスループットを増加する方法
KR20160138579A (ko) 게놈 및 치료학적 적용을 위한 핵산 분자의 클론 복제 및 증폭을 위한 시스템 및 방법
HK40068143A (en) Contiguity preserving transposition
HK40052715A (en) Contiguity preserving transposition
BR112017007912B1 (pt) Transposon de preservação de contiguidade
HK1235082B (en) Contiguity preserving transposition
HK1235082A1 (en) Contiguity preserving transposition

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180926

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191029

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200129

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200327

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200410

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200609

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200907

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200917

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20201110

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20201209

R150 Certificate of patent or registration of utility model

Ref document number: 6808617

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250