JP2010535034A5 - - Google Patents

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Publication number
JP2010535034A5
JP2010535034A5 JP2010520191A JP2010520191A JP2010535034A5 JP 2010535034 A5 JP2010535034 A5 JP 2010535034A5 JP 2010520191 A JP2010520191 A JP 2010520191A JP 2010520191 A JP2010520191 A JP 2010520191A JP 2010535034 A5 JP2010535034 A5 JP 2010535034A5
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JP
Japan
Prior art keywords
primers
primer
template
group
modified polynucleotides
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JP2010520191A
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English (en)
Japanese (ja)
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JP6014805B2 (ja
JP2010535034A (ja
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Priority claimed from PCT/US2008/071771 external-priority patent/WO2009018449A1/en
Publication of JP2010535034A publication Critical patent/JP2010535034A/ja
Publication of JP2010535034A5 publication Critical patent/JP2010535034A5/ja
Application granted granted Critical
Publication of JP6014805B2 publication Critical patent/JP6014805B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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JP2010520191A 2007-07-31 2008-07-31 テイラードマルチサイトコンビナトリアルアセンブリー Expired - Fee Related JP6014805B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95317107P 2007-07-31 2007-07-31
US60/953,171 2007-07-31
PCT/US2008/071771 WO2009018449A1 (en) 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly

Publications (3)

Publication Number Publication Date
JP2010535034A JP2010535034A (ja) 2010-11-18
JP2010535034A5 true JP2010535034A5 (enExample) 2011-09-22
JP6014805B2 JP6014805B2 (ja) 2016-10-26

Family

ID=40304880

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010520191A Expired - Fee Related JP6014805B2 (ja) 2007-07-31 2008-07-31 テイラードマルチサイトコンビナトリアルアセンブリー

Country Status (16)

Country Link
US (1) US9476078B2 (enExample)
EP (2) EP3031919B1 (enExample)
JP (1) JP6014805B2 (enExample)
KR (1) KR101685712B1 (enExample)
CN (2) CN101821402B (enExample)
AU (1) AU2008282101A1 (enExample)
BR (1) BRPI0815046A2 (enExample)
CA (1) CA2693102C (enExample)
DK (1) DK3031919T3 (enExample)
EA (1) EA020657B1 (enExample)
ES (1) ES2701883T3 (enExample)
IL (1) IL202839A0 (enExample)
MX (1) MX353478B (enExample)
NZ (1) NZ582876A (enExample)
WO (1) WO2009018449A1 (enExample)
ZA (1) ZA201000152B (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
AU2010249500B2 (en) 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
JP2014502144A (ja) 2010-10-06 2014-01-30 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド バリアントcbhiポリペプチド
WO2014070856A2 (en) 2012-11-02 2014-05-08 Bp Corporation North America Inc. Thermotolerant beta-glucosidase variants
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
MX2020004318A (es) 2017-10-25 2020-08-13 Basf Se Enzimas beta-amilasa.
CA3083527A1 (en) 2017-12-20 2019-06-27 Basf Se Gene site saturation mutagenesis (gssm) method
WO2019125804A1 (en) 2017-12-20 2019-06-27 Basf Se Nucleases and methods for making and using them
BR112020021692A2 (pt) 2018-04-26 2021-01-26 Basf Se polipeptídeo tendo atividade de lipase, polipeptídeo híbrido, composição, polinucleotídeo variante, método para fabricar a variante de polipeptídeo, e, uso do polipeptídeo
US12351841B2 (en) 2018-05-29 2025-07-08 Basf Se Amylase enzymes
US20220025391A1 (en) 2018-09-07 2022-01-27 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
CN112969792A (zh) 2018-09-07 2021-06-15 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
CN113412332A (zh) 2018-09-07 2021-09-17 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
CN114096271B (zh) 2019-04-23 2024-04-12 巴斯夫欧洲公司 β-淀粉酶变体
MX2022007714A (es) 2019-12-20 2022-07-19 Basf Se Disminucion de la toxicidad de terpenos y aumento de la posible produccion en microorganismos.
BR112022024475A2 (pt) 2020-06-04 2022-12-27 Isobionics B V Beta santaleno sintase sintética, ácido nucleico sintético, cassete de expressão, método para produzir uma composição, célula hospedeira não humana, composição, e, uso de qualquer uma das santaleno sintases sintéticas
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
US20240352490A1 (en) 2021-08-02 2024-10-24 Basf Se Novel production of aroma compounds with ionylideneethane synthases

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5849482A (en) 1988-09-28 1998-12-15 Epoch Pharmaceuticals, Inc. Crosslinking oligonucleotides
DE69233750D1 (de) 1991-04-10 2009-01-02 Scripps Research Inst Bibliotheken heterodimerer Rezeptoren mittels Phagemiden
WO1993013216A1 (en) 1991-12-24 1993-07-08 The President And Fellows Of Harvard College Site-directed mutagenesis of dna
US20030194807A1 (en) 1992-11-02 2003-10-16 Roberto Crea Walk-through mutagenesis
WO1995001364A1 (en) 1993-06-25 1995-01-12 Yale University Chemically modified oligonucleotide for site-directed mutagenesis
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US6479258B1 (en) 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US20020028443A1 (en) 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
US20040023327A1 (en) 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US5789166A (en) 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
GB9701425D0 (en) 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
WO2000028015A1 (fr) 1998-11-11 2000-05-18 Japan Science And Technology Corporation Methode de mutagenese
US6376191B1 (en) 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
JP3859947B2 (ja) 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
FR2813314B1 (fr) * 2000-08-25 2004-05-07 Biomethodes Procede de mutagenese dirigee massive
USPP13969P3 (en) 2001-04-05 2003-07-15 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture Shrub rose plant named ‘Morden Sunrise’
JP4473573B2 (ja) 2001-07-26 2010-06-02 ストラタジーン カリフォルニア 多部位突然変異誘発
DE10148607A1 (de) 2001-10-02 2003-04-10 Max Planck Gesellschaft Methoden zur DNA-Manipulation
US20030129709A1 (en) 2001-11-02 2003-07-10 Olga Makarova Method for site-directed mutagenesis of nucleic acid molecules using a single primer
AU2002364518A1 (en) 2001-12-03 2003-06-17 Diversa Corporation Chromosomal saturation mutagenesis
AUPS263402A0 (en) 2002-05-29 2002-06-20 Young, Lei Targeted amplification of template strand (TATS) technology, and its use in DNA mutagenesis, deletion & insertion
US20060134624A1 (en) 2003-02-06 2006-06-22 Salerno John C Polymerase-based protocols for the introductions of deletions and insertions
US20060228786A1 (en) 2003-02-06 2006-10-12 Salerno John C Polymerase-based protocols for the introduction of deletions and insertions
US6878531B1 (en) 2003-11-10 2005-04-12 Medical College Of Georgia Research Institute Method for multiple site-directed mutagenesis
US20060288786A1 (en) * 2005-03-18 2006-12-28 Flores Andres H L Ultrasonically coupled scanning probe microscope
US20060223066A1 (en) * 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
EP1907584A2 (en) * 2005-06-28 2008-04-09 Agencourt Personal Genomics Corporation Methods of producing and sequencing modified polynucleotides

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