KR101685712B1 - 맞춤형 멀티사이트 조합 어셈블리 - Google Patents

맞춤형 멀티사이트 조합 어셈블리 Download PDF

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KR101685712B1
KR101685712B1 KR1020107003986A KR20107003986A KR101685712B1 KR 101685712 B1 KR101685712 B1 KR 101685712B1 KR 1020107003986 A KR1020107003986 A KR 1020107003986A KR 20107003986 A KR20107003986 A KR 20107003986A KR 101685712 B1 KR101685712 B1 KR 101685712B1
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primers
primer
polynucleotide
template
mutations
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KR20100045494A (ko
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주퀴우 탄
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비피 코포레이션 노쓰 아메리카 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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KR1020107003986A 2007-07-31 2008-07-31 맞춤형 멀티사이트 조합 어셈블리 Expired - Fee Related KR101685712B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95317107P 2007-07-31 2007-07-31
US60/953,171 2007-07-31
PCT/US2008/071771 WO2009018449A1 (en) 2007-07-31 2008-07-31 Tailored multi-site combinatorial assembly

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KR20100045494A KR20100045494A (ko) 2010-05-03
KR101685712B1 true KR101685712B1 (ko) 2016-12-12

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US (1) US9476078B2 (enExample)
EP (2) EP3031919B1 (enExample)
JP (1) JP6014805B2 (enExample)
KR (1) KR101685712B1 (enExample)
CN (2) CN104673784A (enExample)
AU (1) AU2008282101A1 (enExample)
BR (1) BRPI0815046A2 (enExample)
CA (1) CA2693102C (enExample)
DK (1) DK3031919T3 (enExample)
EA (1) EA020657B1 (enExample)
ES (1) ES2701883T3 (enExample)
IL (1) IL202839A0 (enExample)
MX (1) MX353478B (enExample)
NZ (1) NZ582876A (enExample)
WO (1) WO2009018449A1 (enExample)
ZA (1) ZA201000152B (enExample)

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AU2010249500B2 (en) 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
EP2625269A2 (en) 2010-10-06 2013-08-14 BP Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
US20150376590A1 (en) 2012-11-02 2015-12-31 Bp Corporation North America Inc. Thermotolerant Beta-Glucosidase Variants
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
KR20160033711A (ko) 2013-07-25 2016-03-28 바스프 엔자임스 엘엘씨 피타아제
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2019081976A2 (en) 2017-10-25 2019-05-02 Basf Se BETA-AMYLASE ENZYMES
CN111788306A (zh) 2017-12-20 2020-10-16 巴斯夫欧洲公司 核酸酶以及用于制备和使用其的方法
EP3728620A4 (en) * 2017-12-20 2021-11-03 Basf Se GENE SITE SATURATION MUTAGENESIS (GSSM) PROCESS
WO2019206994A1 (en) 2018-04-26 2019-10-31 Basf Se Lipase enzymes
EP3802813A1 (en) 2018-05-29 2021-04-14 Basf Se Amylase enzymes
US20220025391A1 (en) 2018-09-07 2022-01-27 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
WO2020049159A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
CA3110651A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
CN114096271B (zh) 2019-04-23 2024-04-12 巴斯夫欧洲公司 β-淀粉酶变体
WO2021122528A1 (en) 2019-12-20 2021-06-24 Basf Se Decreasing toxicity of terpenes and increasing the production potential in micro-organisms
CN115516102B (zh) 2020-06-04 2025-10-31 艾瑟拜奥尼克斯公司 合成的檀香烯合酶
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
JP2024528957A (ja) 2021-08-02 2024-08-01 ビーエーエスエフ ソシエタス・ヨーロピア イオニリデンエタンシンターゼによる新規な芳香化合物の生成

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CA2693102A1 (en) 2009-02-05
WO2009018449A1 (en) 2009-02-05
MX2010001182A (es) 2010-04-21
DK3031919T3 (en) 2019-01-07
US9476078B2 (en) 2016-10-25
MX353478B (es) 2018-01-15
ES2701883T3 (es) 2019-02-26
KR20100045494A (ko) 2010-05-03
EP3031919B1 (en) 2018-09-19
JP2010535034A (ja) 2010-11-18
US20100216192A1 (en) 2010-08-26
IL202839A0 (en) 2010-06-30
AU2008282101A1 (en) 2009-02-05
EP3031919A1 (en) 2016-06-15
CN104673784A (zh) 2015-06-03
EA020657B1 (ru) 2014-12-30
ZA201000152B (en) 2011-06-29
CA2693102C (en) 2020-03-10
EP2183378A4 (en) 2010-09-22
BRPI0815046A2 (pt) 2014-10-14
EA201070206A1 (ru) 2010-06-30
NZ582876A (en) 2012-07-27
JP6014805B2 (ja) 2016-10-26
EP2183378A1 (en) 2010-05-12
CN101821402A (zh) 2010-09-01
CN101821402B (zh) 2015-03-04

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