JP2004508384A5 - - Google Patents
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- Publication number
- JP2004508384A5 JP2004508384A5 JP2002525179A JP2002525179A JP2004508384A5 JP 2004508384 A5 JP2004508384 A5 JP 2004508384A5 JP 2002525179 A JP2002525179 A JP 2002525179A JP 2002525179 A JP2002525179 A JP 2002525179A JP 2004508384 A5 JP2004508384 A5 JP 2004508384A5
- Authority
- JP
- Japan
- Prior art keywords
- biologically active
- protein
- insoluble
- present
- neutralizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011210 chromatographic step Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 159000000011 group IA salts Chemical class 0.000 description 2
- 210000003000 inclusion body Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10044360.5 | 2000-09-08 | ||
| DE10044360A DE10044360A1 (de) | 2000-09-08 | 2000-09-08 | Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen |
| PCT/EP2001/009552 WO2002020559A1 (de) | 2000-09-08 | 2001-08-18 | Verfahren zur aufreinigung von rekombinanten als unlösliche aggregate exprimierte proteinen |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004508384A JP2004508384A (ja) | 2004-03-18 |
| JP2004508384A5 true JP2004508384A5 (https=) | 2011-08-18 |
| JP5174312B2 JP5174312B2 (ja) | 2013-04-03 |
Family
ID=7655449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002525179A Expired - Fee Related JP5174312B2 (ja) | 2000-09-08 | 2001-08-18 | 不溶性凝集体として発現された組み換えタンパク質を精製する方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6900034B2 (https=) |
| EP (1) | EP1315740B1 (https=) |
| JP (1) | JP5174312B2 (https=) |
| CN (1) | CN1468255A (https=) |
| AT (1) | ATE443712T1 (https=) |
| AU (2) | AU2001287695B2 (https=) |
| CA (1) | CA2421438C (https=) |
| DE (2) | DE10044360A1 (https=) |
| ES (1) | ES2333939T3 (https=) |
| HU (1) | HUP0303554A2 (https=) |
| PL (1) | PL206158B1 (https=) |
| PT (1) | PT1315740E (https=) |
| RU (1) | RU2299214C2 (https=) |
| WO (1) | WO2002020559A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2333951T5 (es) * | 2002-02-27 | 2017-11-16 | Merck Patent Gmbh | Método para producir alergenos principales rBet v 1 hipoalergénicos de abedul |
| CA2487673C (en) * | 2003-12-02 | 2010-11-02 | F. Hoffmann-La Roche Ag | Improved method for the recombinant production and purification of protein kinases |
| EP2323485B1 (en) * | 2008-08-08 | 2013-10-16 | Janssen Pharmaceutica, N.V. | Anti-allergen combinations of calcium and lanthanum salts |
| PL2721051T3 (pl) | 2011-06-15 | 2018-05-30 | Asit Biotech S.A. | Sposób wytwarzania hydrolizowanych alergenów |
| CN104558100A (zh) * | 2015-02-04 | 2015-04-29 | 吉林农业大学 | 包涵体的预处理方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
| US5101018A (en) * | 1989-06-12 | 1992-03-31 | International Minerals & Chemical Corp. | Method for recovering recombinant proteins |
| ATE113060T1 (de) | 1989-12-05 | 1994-11-15 | American Cyanamid Co | Methode zur auflösung und naturation von somatotropin unter verwendung von einer niedrigen harnstoffkonzentration. |
| US5912327A (en) | 1996-09-30 | 1999-06-15 | Human Genome Sciences, Inc. | Method of purifying chemokines from inclusion bodies |
| SE9703531D0 (sv) * | 1997-09-30 | 1997-09-30 | Rudolf Valenta | Non-anaphlactic forms of allergens and their use |
-
2000
- 2000-09-08 DE DE10044360A patent/DE10044360A1/de not_active Withdrawn
-
2001
- 2001-08-18 JP JP2002525179A patent/JP5174312B2/ja not_active Expired - Fee Related
- 2001-08-18 AU AU2001287695A patent/AU2001287695B2/en not_active Ceased
- 2001-08-18 AU AU8769501A patent/AU8769501A/xx active Pending
- 2001-08-18 HU HU0303554A patent/HUP0303554A2/hu unknown
- 2001-08-18 CN CNA018153550A patent/CN1468255A/zh active Pending
- 2001-08-18 WO PCT/EP2001/009552 patent/WO2002020559A1/de not_active Ceased
- 2001-08-18 AT AT01967286T patent/ATE443712T1/de active
- 2001-08-18 ES ES01967286T patent/ES2333939T3/es not_active Expired - Lifetime
- 2001-08-18 CA CA2421438A patent/CA2421438C/en not_active Expired - Fee Related
- 2001-08-18 EP EP01967286A patent/EP1315740B1/de not_active Expired - Lifetime
- 2001-08-18 US US10/363,788 patent/US6900034B2/en not_active Expired - Lifetime
- 2001-08-18 RU RU2003109430/13A patent/RU2299214C2/ru not_active IP Right Cessation
- 2001-08-18 PL PL359713A patent/PL206158B1/pl unknown
- 2001-08-18 DE DE50115119T patent/DE50115119D1/de not_active Expired - Lifetime
- 2001-08-18 PT PT01967286T patent/PT1315740E/pt unknown
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