JP2004504847A5 - - Google Patents
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- Publication number
- JP2004504847A5 JP2004504847A5 JP2002516327A JP2002516327A JP2004504847A5 JP 2004504847 A5 JP2004504847 A5 JP 2004504847A5 JP 2002516327 A JP2002516327 A JP 2002516327A JP 2002516327 A JP2002516327 A JP 2002516327A JP 2004504847 A5 JP2004504847 A5 JP 2004504847A5
- Authority
- JP
- Japan
- Prior art keywords
- codon
- host cell
- replaced
- nucleic acid
- recombinant protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004705 Codon Proteins 0.000 description 32
- 238000000034 method Methods 0.000 description 31
- 108020004707 nucleic acids Proteins 0.000 description 17
- 102000039446 nucleic acids Human genes 0.000 description 17
- 150000007523 nucleic acids Chemical class 0.000 description 17
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 14
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 14
- 108020004566 Transfer RNA Proteins 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000001131 transforming effect Effects 0.000 description 4
- 108700010070 Codon Usage Proteins 0.000 description 3
- 102000002265 Human Growth Hormone Human genes 0.000 description 3
- 108010000521 Human Growth Hormone Proteins 0.000 description 3
- 241000191965 Staphylococcus carnosus Species 0.000 description 3
- 239000000854 Human Growth Hormone Substances 0.000 description 2
- 241000191940 Staphylococcus Species 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000001216 nucleic acid method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10037111.6 | 2000-07-27 | ||
| DE10037111A DE10037111A1 (de) | 2000-07-27 | 2000-07-27 | Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle |
| PCT/EP2001/008660 WO2002010411A2 (en) | 2000-07-27 | 2001-07-21 | Preparation of a recombinant protein in a prokaryotic host cell by improved codon use |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004504847A JP2004504847A (ja) | 2004-02-19 |
| JP2004504847A5 true JP2004504847A5 (https=) | 2008-09-11 |
| JP5044085B2 JP5044085B2 (ja) | 2012-10-10 |
Family
ID=7650734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002516327A Expired - Lifetime JP5044085B2 (ja) | 2000-07-27 | 2001-07-21 | 原核宿主細胞における組換えタンパク質の生産 |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1307569B1 (https=) |
| JP (1) | JP5044085B2 (https=) |
| CN (2) | CN1444655A (https=) |
| AT (1) | ATE325880T1 (https=) |
| AU (1) | AU2001277554A1 (https=) |
| CA (1) | CA2414978C (https=) |
| DE (2) | DE10037111A1 (https=) |
| DK (1) | DK1307569T3 (https=) |
| ES (1) | ES2264700T3 (https=) |
| MX (1) | MXPA03000427A (https=) |
| WO (1) | WO2002010411A2 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001249170A1 (en) * | 2000-03-13 | 2001-09-24 | Aptagen | Method for modifying a nucleic acid |
| US8551469B2 (en) | 2001-02-28 | 2013-10-08 | Superlab Far East Limited | Treatment of tumors and viral diseases with recombinant interferon alpha |
| JP4124639B2 (ja) | 2002-12-17 | 2008-07-23 | 株式会社日本触媒 | 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法 |
| AU2006257286B2 (en) * | 2005-03-09 | 2012-08-16 | Superlab Far East Limited | Uses of recombinant super-compound interferons |
| WO2008027099A2 (en) | 2006-05-30 | 2008-03-06 | Dow Global Technologies Inc. | Rpa optimization |
| JP4886654B2 (ja) * | 2007-10-29 | 2012-02-29 | 日本ケミカルリサーチ株式会社 | ヒト成長ホルモンの効率化された製造方法 |
| CN102286101B (zh) * | 2011-08-08 | 2013-05-08 | 常州亚当生物技术有限公司 | 抗VEGF单克隆抗体Fab片段Vasculizumab及其应用 |
| JP6224077B2 (ja) | 2012-04-17 | 2017-11-01 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改変された核酸を用いてポリペプチドを発現させるための方法 |
| CN107723287B (zh) * | 2016-08-12 | 2021-07-06 | 中国科学院天津工业生物技术研究所 | 一种增强丝蛋白生产制备的表达系统 |
| CN108456703B (zh) * | 2017-02-20 | 2022-01-14 | 复旦大学 | 一种异源表达埃博霉素的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU618640B2 (en) * | 1988-11-11 | 1992-01-02 | Boehringer Mannheim Gmbh | Process for the expression of a recombinant gene |
| JPH05284973A (ja) * | 1992-04-13 | 1993-11-02 | Kurita Water Ind Ltd | 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法 |
| JP3556965B2 (ja) * | 1993-11-08 | 2004-08-25 | 天野エンザイム株式会社 | 糸状菌および酵母で使用可能なポリペプチド分泌発現用プラスミドおよびそれを用いたポリペプチドの製造法 |
| WO1998035029A1 (en) * | 1997-02-07 | 1998-08-13 | Vanderbilt University | Synthetic genes for recombinant mycobacterium proteins |
| AUPP807899A0 (en) * | 1999-01-08 | 1999-02-04 | University Of Queensland, The | Codon utilization |
| JP2000175687A (ja) * | 1998-12-16 | 2000-06-27 | Japan Science & Technology Corp | ネオカルチノスタチン・アポタンパク質合成遺伝子 |
| DE60042396D1 (de) * | 1999-01-27 | 2009-07-30 | Carstens Carsten Peter | Hohe expression eines heterologen proteins mit seltenen codons |
-
2000
- 2000-07-27 DE DE10037111A patent/DE10037111A1/de not_active Withdrawn
-
2001
- 2001-07-21 CA CA002414978A patent/CA2414978C/en not_active Expired - Lifetime
- 2001-07-21 AU AU2001277554A patent/AU2001277554A1/en not_active Abandoned
- 2001-07-21 DE DE60119535T patent/DE60119535T2/de not_active Expired - Lifetime
- 2001-07-21 WO PCT/EP2001/008660 patent/WO2002010411A2/en not_active Ceased
- 2001-07-21 ES ES01955373T patent/ES2264700T3/es not_active Expired - Lifetime
- 2001-07-21 AT AT01955373T patent/ATE325880T1/de active
- 2001-07-21 JP JP2002516327A patent/JP5044085B2/ja not_active Expired - Lifetime
- 2001-07-21 CN CN01813410A patent/CN1444655A/zh active Pending
- 2001-07-21 EP EP01955373A patent/EP1307569B1/en not_active Expired - Lifetime
- 2001-07-21 MX MXPA03000427A patent/MXPA03000427A/es active IP Right Grant
- 2001-07-21 CN CN2009100035743A patent/CN101525628B/zh not_active Expired - Lifetime
- 2001-07-21 DK DK01955373T patent/DK1307569T3/da active
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