ATE296354T1 - Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen - Google Patents
Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen genInfo
- Publication number
- ATE296354T1 ATE296354T1 AT02740737T AT02740737T ATE296354T1 AT E296354 T1 ATE296354 T1 AT E296354T1 AT 02740737 T AT02740737 T AT 02740737T AT 02740737 T AT02740737 T AT 02740737T AT E296354 T1 ATE296354 T1 AT E296354T1
- Authority
- AT
- Austria
- Prior art keywords
- gene
- vector
- antibiotic resistance
- essential
- cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0102204A SE0102204D0 (sv) | 2001-06-21 | 2001-06-21 | New method |
PCT/EP2002/006780 WO2003000881A2 (en) | 2001-06-21 | 2002-06-19 | Method of plasmid stabilisation by in vivo deleting the antibiotic resistence and selecting with an essential gene |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE296354T1 true ATE296354T1 (de) | 2005-06-15 |
Family
ID=20284558
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02740737T ATE296354T1 (de) | 2001-06-21 | 2002-06-19 | Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen |
Country Status (10)
Country | Link |
---|---|
US (1) | US20040234984A1 (de) |
EP (1) | EP1404847B1 (de) |
CN (1) | CN1249244C (de) |
AT (1) | ATE296354T1 (de) |
AU (1) | AU2002314183A1 (de) |
DE (1) | DE60204317T2 (de) |
ES (1) | ES2240758T3 (de) |
SE (1) | SE0102204D0 (de) |
WO (1) | WO2003000881A2 (de) |
ZA (1) | ZA200309767B (de) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2868422B1 (fr) * | 2004-03-31 | 2006-07-14 | Aventis Pharma Sa | Nouveaux derives pyrrolo(2,3-b) pyridine, leur preparation et leur utilisation pharmaceutique comme inhibiteurs de kinases |
TWI311152B (en) * | 2004-09-17 | 2009-06-21 | Boehringer Ingelheim Rcv Gmbh & Co K | Host-vector system for antibiotic-free cole1 plasmid propagation |
US20140106398A1 (en) * | 2011-03-11 | 2014-04-17 | Dsm Ip Assets B.V. | Vector-host system |
US10987432B2 (en) * | 2013-09-05 | 2021-04-27 | The University Of Hong Kong | Therapeutic delivery and expression system, methods and uses thereof |
CA2933975C (en) * | 2013-12-20 | 2022-06-21 | Basf Se | A process for production of a protein of interest in a microbial host organism |
US10829508B2 (en) | 2015-12-18 | 2020-11-10 | Glycom A/S | Fermentative production of oligosaccharides |
IL266237B (en) | 2016-11-01 | 2022-08-01 | Novo Nordisk As | Tolerogenic DNA composition |
RU2712838C1 (ru) * | 2018-09-04 | 2020-01-31 | Генетик Диагностик Энд Терапи 21 Лтд | Генотерапевтический ДНК-вектор GDTT1.8NAS12, способ его получения, штамм Escherichia coli JM110-NAS, способ его получения, штамм Escherichia coli JM110-NAS/GDTT1.8NAS12, несущий генотерапевтический ДНК-вектор GDTT1.8NAS12, способ его получения, способ производства в промышленных масштабах генотерапевтического ДНК-вектора |
US11279745B2 (en) | 2019-04-26 | 2022-03-22 | Novo Nordisk A/S | Tolerogenic DNA vaccine |
EP4341407A1 (de) | 2021-05-20 | 2024-03-27 | Gen-H Genetic Engineering Heidelberg GmbH | Mikroorganismusstamm und verfahren zur antibiotikafreien fermentation auf plasmidbasis |
WO2023166132A1 (en) | 2022-03-04 | 2023-09-07 | Gen-H Genetic Engineering Heidelberg Gmbh | Microorganism strain for antibiotic-free plasmid-based fermentation and method for generation thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6291245B1 (en) * | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
EP0972838B1 (de) * | 1998-07-15 | 2004-09-15 | Roche Diagnostics GmbH | Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie |
FR2784394B1 (fr) * | 1998-10-09 | 2001-01-05 | Lesaffre & Cie | Cassette d'integration-excision |
-
2001
- 2001-06-21 SE SE0102204A patent/SE0102204D0/xx unknown
-
2002
- 2002-06-19 DE DE60204317T patent/DE60204317T2/de not_active Expired - Lifetime
- 2002-06-19 US US10/481,038 patent/US20040234984A1/en not_active Abandoned
- 2002-06-19 AU AU2002314183A patent/AU2002314183A1/en not_active Abandoned
- 2002-06-19 WO PCT/EP2002/006780 patent/WO2003000881A2/en not_active Application Discontinuation
- 2002-06-19 CN CN02812556.8A patent/CN1249244C/zh not_active Expired - Fee Related
- 2002-06-19 EP EP02740737A patent/EP1404847B1/de not_active Expired - Lifetime
- 2002-06-19 AT AT02740737T patent/ATE296354T1/de not_active IP Right Cessation
- 2002-06-19 ES ES02740737T patent/ES2240758T3/es not_active Expired - Lifetime
-
2003
- 2003-12-17 ZA ZA2003/09767A patent/ZA200309767B/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN1518599A (zh) | 2004-08-04 |
CN1249244C (zh) | 2006-04-05 |
ES2240758T3 (es) | 2005-10-16 |
DE60204317D1 (de) | 2005-06-30 |
US20040234984A1 (en) | 2004-11-25 |
WO2003000881A3 (en) | 2003-05-01 |
EP1404847B1 (de) | 2005-05-25 |
AU2002314183A1 (en) | 2003-01-08 |
SE0102204D0 (sv) | 2001-06-21 |
WO2003000881A2 (en) | 2003-01-03 |
ZA200309767B (en) | 2005-07-27 |
EP1404847A2 (de) | 2004-04-07 |
DE60204317T2 (de) | 2006-01-26 |
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