DE10037111A1 - Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle - Google Patents
Herstellung eines rekombinanten Proteins in einer prokaryontischen WirtszelleInfo
- Publication number
- DE10037111A1 DE10037111A1 DE10037111A DE10037111A DE10037111A1 DE 10037111 A1 DE10037111 A1 DE 10037111A1 DE 10037111 A DE10037111 A DE 10037111A DE 10037111 A DE10037111 A DE 10037111A DE 10037111 A1 DE10037111 A1 DE 10037111A1
- Authority
- DE
- Germany
- Prior art keywords
- host cell
- replaced
- codon
- nucleic acid
- codons
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims description 35
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- 238000000034 method Methods 0.000 claims abstract description 79
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- 108020004566 Transfer RNA Proteins 0.000 claims abstract description 22
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- 239000013598 vector Substances 0.000 claims description 7
- 108700010070 Codon Usage Proteins 0.000 claims description 6
- 241000191940 Staphylococcus Species 0.000 claims description 6
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10037111A DE10037111A1 (de) | 2000-07-27 | 2000-07-27 | Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle |
| PCT/EP2001/008660 WO2002010411A2 (en) | 2000-07-27 | 2001-07-21 | Preparation of a recombinant protein in a prokaryotic host cell by improved codon use |
| MXPA03000427A MXPA03000427A (es) | 2000-07-27 | 2001-07-21 | Preparacion de una proteina recombinante en una celula huesped procariotica. |
| JP2002516327A JP5044085B2 (ja) | 2000-07-27 | 2001-07-21 | 原核宿主細胞における組換えタンパク質の生産 |
| EP01955373A EP1307569B1 (en) | 2000-07-27 | 2001-07-21 | Preparation of a recombinant protein in a prokaryotic host cell by improved codon use |
| CA002414978A CA2414978C (en) | 2000-07-27 | 2001-07-21 | Preparation of a recombinant protein in a prokaryotic host cell |
| DK01955373T DK1307569T3 (da) | 2000-07-27 | 2001-07-21 | Fremstilling af rekombinant protein i en prokaryot værtscelle ved forbedret codonanvendelse |
| ES01955373T ES2264700T3 (es) | 2000-07-27 | 2001-07-21 | Preparacion de una proteina recombinante en una celula huesped procariotica mediante el uso de un codon mejorado. |
| AT01955373T ATE325880T1 (de) | 2000-07-27 | 2001-07-21 | Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung |
| AU2001277554A AU2001277554A1 (en) | 2000-07-27 | 2001-07-21 | Preparation of a recombinant protein in a prokaryotic host cell by improved codon use |
| CN2009100035743A CN101525628B (zh) | 2000-07-27 | 2001-07-21 | 在原核宿主细胞中制备重组蛋白 |
| CN01813410A CN1444655A (zh) | 2000-07-27 | 2001-07-21 | 在原核宿主细胞中制备重组蛋白 |
| DE60119535T DE60119535T2 (de) | 2000-07-27 | 2001-07-21 | Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung |
| US09/916,229 US6821755B2 (en) | 2000-07-27 | 2001-07-27 | Preparation of a recombinant protein in a prokaryotic host cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10037111A DE10037111A1 (de) | 2000-07-27 | 2000-07-27 | Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10037111A1 true DE10037111A1 (de) | 2002-02-07 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10037111A Withdrawn DE10037111A1 (de) | 2000-07-27 | 2000-07-27 | Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle |
| DE60119535T Expired - Lifetime DE60119535T2 (de) | 2000-07-27 | 2001-07-21 | Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60119535T Expired - Lifetime DE60119535T2 (de) | 2000-07-27 | 2001-07-21 | Herstellung eines rekombinanten proteins in prokaryotischen wirtszellen durch kodonsoptimisierung |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1307569B1 (https=) |
| JP (1) | JP5044085B2 (https=) |
| CN (2) | CN1444655A (https=) |
| AT (1) | ATE325880T1 (https=) |
| AU (1) | AU2001277554A1 (https=) |
| CA (1) | CA2414978C (https=) |
| DE (2) | DE10037111A1 (https=) |
| DK (1) | DK1307569T3 (https=) |
| ES (1) | ES2264700T3 (https=) |
| MX (1) | MXPA03000427A (https=) |
| WO (1) | WO2002010411A2 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001249170A1 (en) * | 2000-03-13 | 2001-09-24 | Aptagen | Method for modifying a nucleic acid |
| US8551469B2 (en) | 2001-02-28 | 2013-10-08 | Superlab Far East Limited | Treatment of tumors and viral diseases with recombinant interferon alpha |
| JP4124639B2 (ja) | 2002-12-17 | 2008-07-23 | 株式会社日本触媒 | 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法 |
| AU2006257286B2 (en) * | 2005-03-09 | 2012-08-16 | Superlab Far East Limited | Uses of recombinant super-compound interferons |
| WO2008027099A2 (en) | 2006-05-30 | 2008-03-06 | Dow Global Technologies Inc. | Rpa optimization |
| JP4886654B2 (ja) * | 2007-10-29 | 2012-02-29 | 日本ケミカルリサーチ株式会社 | ヒト成長ホルモンの効率化された製造方法 |
| CN102286101B (zh) * | 2011-08-08 | 2013-05-08 | 常州亚当生物技术有限公司 | 抗VEGF单克隆抗体Fab片段Vasculizumab及其应用 |
| JP6224077B2 (ja) | 2012-04-17 | 2017-11-01 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改変された核酸を用いてポリペプチドを発現させるための方法 |
| CN107723287B (zh) * | 2016-08-12 | 2021-07-06 | 中国科学院天津工业生物技术研究所 | 一种增强丝蛋白生产制备的表达系统 |
| CN108456703B (zh) * | 2017-02-20 | 2022-01-14 | 复旦大学 | 一种异源表达埃博霉素的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU618640B2 (en) * | 1988-11-11 | 1992-01-02 | Boehringer Mannheim Gmbh | Process for the expression of a recombinant gene |
| JPH05284973A (ja) * | 1992-04-13 | 1993-11-02 | Kurita Water Ind Ltd | 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法 |
| JP3556965B2 (ja) * | 1993-11-08 | 2004-08-25 | 天野エンザイム株式会社 | 糸状菌および酵母で使用可能なポリペプチド分泌発現用プラスミドおよびそれを用いたポリペプチドの製造法 |
| WO1998035029A1 (en) * | 1997-02-07 | 1998-08-13 | Vanderbilt University | Synthetic genes for recombinant mycobacterium proteins |
| AUPP807899A0 (en) * | 1999-01-08 | 1999-02-04 | University Of Queensland, The | Codon utilization |
| JP2000175687A (ja) * | 1998-12-16 | 2000-06-27 | Japan Science & Technology Corp | ネオカルチノスタチン・アポタンパク質合成遺伝子 |
| DE60042396D1 (de) * | 1999-01-27 | 2009-07-30 | Carstens Carsten Peter | Hohe expression eines heterologen proteins mit seltenen codons |
-
2000
- 2000-07-27 DE DE10037111A patent/DE10037111A1/de not_active Withdrawn
-
2001
- 2001-07-21 CA CA002414978A patent/CA2414978C/en not_active Expired - Lifetime
- 2001-07-21 AU AU2001277554A patent/AU2001277554A1/en not_active Abandoned
- 2001-07-21 DE DE60119535T patent/DE60119535T2/de not_active Expired - Lifetime
- 2001-07-21 WO PCT/EP2001/008660 patent/WO2002010411A2/en not_active Ceased
- 2001-07-21 ES ES01955373T patent/ES2264700T3/es not_active Expired - Lifetime
- 2001-07-21 AT AT01955373T patent/ATE325880T1/de active
- 2001-07-21 JP JP2002516327A patent/JP5044085B2/ja not_active Expired - Lifetime
- 2001-07-21 CN CN01813410A patent/CN1444655A/zh active Pending
- 2001-07-21 EP EP01955373A patent/EP1307569B1/en not_active Expired - Lifetime
- 2001-07-21 MX MXPA03000427A patent/MXPA03000427A/es active IP Right Grant
- 2001-07-21 CN CN2009100035743A patent/CN101525628B/zh not_active Expired - Lifetime
- 2001-07-21 DK DK01955373T patent/DK1307569T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| ES2264700T3 (es) | 2007-01-16 |
| CN101525628A (zh) | 2009-09-09 |
| CA2414978C (en) | 2009-02-24 |
| WO2002010411A3 (en) | 2002-07-18 |
| CN101525628B (zh) | 2011-08-24 |
| MXPA03000427A (es) | 2003-10-06 |
| EP1307569A2 (en) | 2003-05-07 |
| JP5044085B2 (ja) | 2012-10-10 |
| DK1307569T3 (da) | 2006-09-04 |
| DE60119535D1 (de) | 2006-06-14 |
| WO2002010411A2 (en) | 2002-02-07 |
| CA2414978A1 (en) | 2002-02-07 |
| EP1307569B1 (en) | 2006-05-10 |
| JP2004504847A (ja) | 2004-02-19 |
| AU2001277554A1 (en) | 2002-02-13 |
| ATE325880T1 (de) | 2006-06-15 |
| CN1444655A (zh) | 2003-09-24 |
| DE60119535T2 (de) | 2006-09-07 |
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