CN1444655A - 在原核宿主细胞中制备重组蛋白 - Google Patents

在原核宿主细胞中制备重组蛋白 Download PDF

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Publication number
CN1444655A
CN1444655A CN01813410A CN01813410A CN1444655A CN 1444655 A CN1444655 A CN 1444655A CN 01813410 A CN01813410 A CN 01813410A CN 01813410 A CN01813410 A CN 01813410A CN 1444655 A CN1444655 A CN 1444655A
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CN
China
Prior art keywords
host cell
replaced
codon
codons
nucleic acid
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Pending
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CN01813410A
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English (en)
Chinese (zh)
Inventor
罗尔夫-冈瑟·沃纳
克劳斯·伯格曼
弗里德里克·戈茨
安德烈亚斯·佩希尔
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Boehringer Ingelheim International GmbH
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Boehringer Ingelheim International GmbH
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Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of CN1444655A publication Critical patent/CN1444655A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN01813410A 2000-07-27 2001-07-21 在原核宿主细胞中制备重组蛋白 Pending CN1444655A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10037111.6 2000-07-27
DE10037111A DE10037111A1 (de) 2000-07-27 2000-07-27 Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN2009100035743A Division CN101525628B (zh) 2000-07-27 2001-07-21 在原核宿主细胞中制备重组蛋白

Publications (1)

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CN1444655A true CN1444655A (zh) 2003-09-24

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ID=7650734

Family Applications (2)

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CN01813410A Pending CN1444655A (zh) 2000-07-27 2001-07-21 在原核宿主细胞中制备重组蛋白
CN2009100035743A Expired - Lifetime CN101525628B (zh) 2000-07-27 2001-07-21 在原核宿主细胞中制备重组蛋白

Family Applications After (1)

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CN2009100035743A Expired - Lifetime CN101525628B (zh) 2000-07-27 2001-07-21 在原核宿主细胞中制备重组蛋白

Country Status (11)

Country Link
EP (1) EP1307569B1 (https=)
JP (1) JP5044085B2 (https=)
CN (2) CN1444655A (https=)
AT (1) ATE325880T1 (https=)
AU (1) AU2001277554A1 (https=)
CA (1) CA2414978C (https=)
DE (2) DE10037111A1 (https=)
DK (1) DK1307569T3 (https=)
ES (1) ES2264700T3 (https=)
MX (1) MXPA03000427A (https=)
WO (1) WO2002010411A2 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723287A (zh) * 2016-08-12 2018-02-23 中国科学院天津工业生物技术研究所 一种增强丝蛋白生产制备的表达系统
WO2018149282A1 (zh) * 2017-02-20 2018-08-23 复旦大学 一种异源表达埃博霉素的方法

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001249170A1 (en) * 2000-03-13 2001-09-24 Aptagen Method for modifying a nucleic acid
US8551469B2 (en) 2001-02-28 2013-10-08 Superlab Far East Limited Treatment of tumors and viral diseases with recombinant interferon alpha
JP4124639B2 (ja) 2002-12-17 2008-07-23 株式会社日本触媒 大腸菌を用いたｓ−ヒドロキシニトリルリアーゼの製造方法
AU2006257286B2 (en) * 2005-03-09 2012-08-16 Superlab Far East Limited Uses of recombinant super-compound interferons
WO2008027099A2 (en) 2006-05-30 2008-03-06 Dow Global Technologies Inc. Rpa optimization
JP4886654B2 (ja) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 ヒト成長ホルモンの効率化された製造方法
CN102286101B (zh) * 2011-08-08 2013-05-08 常州亚当生物技术有限公司 抗VEGF单克隆抗体Fab片段Vasculizumab及其应用
JP6224077B2 (ja) 2012-04-17 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改変された核酸を用いてポリペプチドを発現させるための方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU618640B2 (en) * 1988-11-11 1992-01-02 Boehringer Mannheim Gmbh Process for the expression of a recombinant gene
JPH05284973A (ja) * 1992-04-13 1993-11-02 Kurita Water Ind Ltd 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法
JP3556965B2 (ja) * 1993-11-08 2004-08-25 天野エンザイム株式会社 糸状菌および酵母で使用可能なポリペプチド分泌発現用プラスミドおよびそれを用いたポリペプチドの製造法
WO1998035029A1 (en) * 1997-02-07 1998-08-13 Vanderbilt University Synthetic genes for recombinant mycobacterium proteins
AUPP807899A0 (en) * 1999-01-08 1999-02-04 University Of Queensland, The Codon utilization
JP2000175687A (ja) * 1998-12-16 2000-06-27 Japan Science & Technology Corp ネオカルチノスタチン・アポタンパク質合成遺伝子
DE60042396D1 (de) * 1999-01-27 2009-07-30 Carstens Carsten Peter Hohe expression eines heterologen proteins mit seltenen codons

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723287A (zh) * 2016-08-12 2018-02-23 中国科学院天津工业生物技术研究所 一种增强丝蛋白生产制备的表达系统
CN107723287B (zh) * 2016-08-12 2021-07-06 中国科学院天津工业生物技术研究所 一种增强丝蛋白生产制备的表达系统
WO2018149282A1 (zh) * 2017-02-20 2018-08-23 复旦大学 一种异源表达埃博霉素的方法
CN108456703A (zh) * 2017-02-20 2018-08-28 复旦大学 一种异源表达埃博霉素的方法
CN108456703B (zh) * 2017-02-20 2022-01-14 复旦大学 一种异源表达埃博霉素的方法

Also Published As

Publication number Publication date
ES2264700T3 (es) 2007-01-16
DE10037111A1 (de) 2002-02-07
CN101525628A (zh) 2009-09-09
CA2414978C (en) 2009-02-24
WO2002010411A3 (en) 2002-07-18
CN101525628B (zh) 2011-08-24
MXPA03000427A (es) 2003-10-06
EP1307569A2 (en) 2003-05-07
JP5044085B2 (ja) 2012-10-10
DK1307569T3 (da) 2006-09-04
DE60119535D1 (de) 2006-06-14
WO2002010411A2 (en) 2002-02-07
CA2414978A1 (en) 2002-02-07
EP1307569B1 (en) 2006-05-10
JP2004504847A (ja) 2004-02-19
AU2001277554A1 (en) 2002-02-13
ATE325880T1 (de) 2006-06-15
DE60119535T2 (de) 2006-09-07

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