AU2001277554A1 - Preparation of a recombinant protein in a prokaryotic host cell by improved codon use - Google Patents

Preparation of a recombinant protein in a prokaryotic host cell by improved codon use

Info

Publication number
AU2001277554A1
AU2001277554A1 AU2001277554A AU7755401A AU2001277554A1 AU 2001277554 A1 AU2001277554 A1 AU 2001277554A1 AU 2001277554 A AU2001277554 A AU 2001277554A AU 7755401 A AU7755401 A AU 7755401A AU 2001277554 A1 AU2001277554 A1 AU 2001277554A1
Authority
AU
Australia
Prior art keywords
host cell
preparation
prokaryotic host
recombinant protein
codon use
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001277554A
Inventor
Klaus Bergemann
Friedrich Gotz
Andreas Peschel
Rolf-Gunther Werner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of AU2001277554A1 publication Critical patent/AU2001277554A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)

Abstract

The invention relates to advantageous processes for preparing heterologous proteins in prokaryotic host cells by improved codon use and/or expression of tRNAs which code for codons occurring rarely in said host cell.
AU2001277554A 2000-07-27 2001-07-21 Preparation of a recombinant protein in a prokaryotic host cell by improved codon use Abandoned AU2001277554A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10037111A DE10037111A1 (en) 2000-07-27 2000-07-27 Production of a recombinant protein in a prokaryotic host cell
DE10037111.6 2000-07-27
DE10037111 2000-07-27
PCT/EP2001/008660 WO2002010411A2 (en) 2000-07-27 2001-07-21 Preparation of a recombinant protein in a prokaryotic host cell by improved codon use

Publications (1)

Publication Number Publication Date
AU2001277554A1 true AU2001277554A1 (en) 2002-02-13

Family

ID=7650734

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001277554A Abandoned AU2001277554A1 (en) 2000-07-27 2001-07-21 Preparation of a recombinant protein in a prokaryotic host cell by improved codon use

Country Status (11)

Country Link
EP (1) EP1307569B1 (en)
JP (1) JP5044085B2 (en)
CN (2) CN1444655A (en)
AT (1) ATE325880T1 (en)
AU (1) AU2001277554A1 (en)
CA (1) CA2414978C (en)
DE (2) DE10037111A1 (en)
DK (1) DK1307569T3 (en)
ES (1) ES2264700T3 (en)
MX (1) MXPA03000427A (en)
WO (1) WO2002010411A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001249170A1 (en) * 2000-03-13 2001-09-24 Aptagen Method for modifying a nucleic acid
US8551469B2 (en) 2001-02-28 2013-10-08 Superlab Far East Limited Treatment of tumors and viral diseases with recombinant interferon alpha
JP4124639B2 (en) 2002-12-17 2008-07-23 株式会社日本触媒 Method for producing S-hydroxynitrile lyase using E. coli
US20080124302A1 (en) * 2005-03-09 2008-05-29 Guangwen Wei Uses of Recombinant Super-Compound Interferons
CN101495644B (en) 2006-05-30 2012-11-07 陶氏环球技术有限责任公司 Codon optimization method
JP4886654B2 (en) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 Efficient production method of human growth hormone
CN102286101B (en) * 2011-08-08 2013-05-08 常州亚当生物技术有限公司 Fab (fragment of antigen binding) fragment Vasculizumab of anti-VEGF (vascular endothelial growth factor) monoclonal antibody and application thereof
CN104245937B (en) * 2012-04-17 2021-09-21 弗·哈夫曼-拉罗切有限公司 Methods of expressing polypeptides using modified nucleic acids
CN107723287B (en) * 2016-08-12 2021-07-06 中国科学院天津工业生物技术研究所 Expression system for enhancing production and preparation of silk protein
CN108456703B (en) * 2017-02-20 2022-01-14 复旦大学 Method for heterogeneously expressing epothilone

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU618640B2 (en) * 1988-11-11 1992-01-02 Boehringer Mannheim Gmbh Process for the expression of a recombinant gene
JPH05284973A (en) * 1992-04-13 1993-11-02 Kurita Water Ind Ltd Recombinant plasmid and process for secreting and producing foreign protein using the plasmid as vector
JP3556965B2 (en) * 1993-11-08 2004-08-25 天野エンザイム株式会社 Plasmid for polypeptide secretion and expression usable in filamentous fungi and yeast and method for producing polypeptide using the same
WO1998035029A1 (en) * 1997-02-07 1998-08-13 Vanderbilt University Synthetic genes for recombinant mycobacterium proteins
AUPP807899A0 (en) * 1999-01-08 1999-02-04 University Of Queensland, The Codon utilization
JP2000175687A (en) * 1998-12-16 2000-06-27 Japan Science & Technology Corp Synthetic gene for neocarzinostatin apo protein
EP1147211B1 (en) * 1999-01-27 2009-06-17 Stratagene California High level expression of a heterologous protein having rare codons

Also Published As

Publication number Publication date
CN101525628B (en) 2011-08-24
ES2264700T3 (en) 2007-01-16
MXPA03000427A (en) 2003-10-06
WO2002010411A3 (en) 2002-07-18
ATE325880T1 (en) 2006-06-15
CA2414978A1 (en) 2002-02-07
DE60119535T2 (en) 2006-09-07
WO2002010411A2 (en) 2002-02-07
CN1444655A (en) 2003-09-24
DE60119535D1 (en) 2006-06-14
EP1307569A2 (en) 2003-05-07
CN101525628A (en) 2009-09-09
DK1307569T3 (en) 2006-09-04
JP5044085B2 (en) 2012-10-10
CA2414978C (en) 2009-02-24
DE10037111A1 (en) 2002-02-07
JP2004504847A (en) 2004-02-19
EP1307569B1 (en) 2006-05-10

Similar Documents

Publication Publication Date Title
WO1994018332A3 (en) Anthrax toxin fusion proteins and uses thereof
AU2001296600A1 (en) Mini-dystrophin nucleic acid and peptide sequences
WO2000020555A3 (en) Transformation system in the field of filamentous fungal hosts: in chrysosporium
IL140938A (en) Dna sequences encoding polypeptides capable of binding to ldcam, polypeptides encoded by them, fusion proteins comprising the polypeptides and a process for the preparation of the polypeptides
WO2006016370A3 (en) Soluble fusion proteins comprising heterologous polypeptides
HK1065335A1 (en) Fusion protein for the secretion of a protein of interest into the supernatant of the bacterial culture
EP1304382A3 (en) Production of peptides in plants as viral coat protein fusions
WO2005071088A3 (en) Recombinant nucleic acid molecules encoding fusion proteins comprising antigens and bacterial secretory signal polypeptides, expression cassettes, and bacteria, and methods of use thereof
WO1998056930A3 (en) Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
EP1092774A3 (en) Borrelia burgdorferi antigen OspA, gene encoding it and uses thereof
WO2005003313A3 (en) Methods and compositions for enhanced protein expression and purification
WO2003057174A3 (en) Methods and compositions for protein expression and purification
AU2001277554A1 (en) Preparation of a recombinant protein in a prokaryotic host cell by improved codon use
DE60331224D1 (en) SUBTILISIN CARLSBERG PROTEINS WITH REDUCED IMMUNOGENICITY
AU2001259217A1 (en) Human pellino polypeptides
MXPA03004162A (en) Methods for large scale protein production in prokaryotes.
AU1093597A (en) Vector for expression of n-terminally extended proteins in yeast cell
WO2003004636A3 (en) Expression vectors encoding bacteriophage signal peptides
EP1007627A4 (en) Novel extracellular serine protease
TW200604207A (en) A superantigen fusion protein and the use thereof
WO2003057708A3 (en) Fusion proteins
WO2001020005A8 (en) Regulatory sequences and expression cassettes for yeasts, especially for kluyveromyces
HUP0500099A2 (en) Glucuronyl c5-epimerase, dna encoding the same and uses thereof
EP0869175A3 (en) Cytochrome C and its gene
WO2002038769A3 (en) Dna sequences, which code for optimised eukaryotic hpv 16-l1 and hpv 16-l2