JP2004141162A - 酵素の安定化方法および組成物 - Google Patents
酵素の安定化方法および組成物 Download PDFInfo
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- JP2004141162A JP2004141162A JP2003345233A JP2003345233A JP2004141162A JP 2004141162 A JP2004141162 A JP 2004141162A JP 2003345233 A JP2003345233 A JP 2003345233A JP 2003345233 A JP2003345233 A JP 2003345233A JP 2004141162 A JP2004141162 A JP 2004141162A
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Abstract
【解決手段】 安定化剤として糖類を共存させることを特徴とする、防腐剤共存下における酵素の安定化方法および安定化組成物。さらには、防腐剤としてイソチアゾリン系化合物を用いる上記安定化方法および安定化組成物。さらには、糖類の添加濃度が防腐剤に対して0.05〜500倍量(W/W)である上記安定化方法および安定化組成物。
Description
(1)安定化剤として糖類を共存させることを特徴とする、防腐剤共存下における酵 素の安定化方法。
(2)防腐剤としてイソチアゾリン系化合物を用いる請求項1記載の酵素の安定化方 法。
(3)糖類の添加濃度が防腐剤に対して0.05〜500倍量(W/W)である請求 項1または2記載の酵素の安定化方法。
(4)安定化剤として糖類を共存させることを特徴とする、防腐剤共存下における酵 素の安定化組成物。
(5)防腐剤としてイソチアゾリン系化合物を用いた請求項4記載の酵素の安定化組 成物。
(6)糖類の添加濃度が防腐剤に対して0.05〜500倍量(W/W)である請求 項4または5記載の酵素の安定化組成物。
〈測定試薬〉
(1)基質液
0.3M グリセロール溶液
(2)発色液
100mM HEPES緩衝液、pH7.9
2mM ATP
1mM 塩化マグネシウム
0.1g/L フェノール
0.2g/L 4-アミノアンチピリン
8KU/L グリセロリン酸オキシダーゼ
5.5KU/L ペルオキシダーゼ
〈測定条件〉
(1)発色液3mLをキュベット(d=1cm)に採り、酵素液0.1mLを加え、 ゆるやかに混和後、37℃で約5分間予備加温する。
(2)グリセロール溶液0.05mLを添加し、ゆるやかに混和後、水を対照に37 ℃に制御された分光光度計で500nmの吸光度変化を3〜4分間記録し、その初期直 線部分から1分間当たりの吸光度を求める(ΔODtest)。
(3)盲検は発色液3mLに酵素溶液の代わりに酵素希釈液(20mM Kリン酸緩 衝液、pH7.5)を0.1mL加え、上記同様に操作を行なって、1分間当たりの吸 光度変化を求める(ΔODblank)。
(実施例1)
グリセロールキナーゼ(東洋紡績製GYK−311)3U/mL、各種糖類5g/L含有し、防腐剤としてプロクリン300(スペルコ製)を0.3mL/Lとなるように調製した下記試薬にて35℃で14日間保存し、残存活性(溶解直後の活性値に対する保存後の活性値の割合)を検討した。
下記組成からなる試薬をそれぞれ調製した。
HEPES−NaOH 50mM pH7.5
グリセロールキナーゼ(東洋紡社製GYK−311) 3U/mL
プロクリン300 0.3mL/L
各種糖類(名称は表に記載) 5g/L
グリセロールキナーゼ(東洋紡績製GYK−311)3U/mL、プロクリン300(ロシュ製)0.3mL/L含有試薬に、シュークロース0〜5g/Lとなるように添加濃度を変えて調製した試薬にて35℃で14日間保存し、残存活性(溶解直後の活性値に対する保存後の活性値の割合)を検討した。
グリセロールキナーゼ(東洋紡製GYK−311)12U/mL、プロクリン300(スペルコ製)0.3mL/L、シュークロース5g/L含有試薬を9℃にて6週間保存し、残存活性(溶解直後の活性値に対する保存後の活性値の割合)を検討した。
Claims (6)
- 安定化剤として糖類を共存させることを特徴とする、防腐剤共存下における酵素の安定化方法。
- 防腐剤としてイソチアゾリン系化合物を用いる請求項1記載の酵素の安定化方法。
- 糖類の添加濃度が防腐剤に対して0.05〜500倍量(W/W)である請求項1または2記載の酵素の安定化方法。
- 安定化剤として糖類を共存させることを特徴とする、防腐剤共存下における酵素の安定化組成物。
- 防腐剤としてイソチアゾリン系化合物を用いた請求項4記載の酵素の安定化組成物。
- 糖類の添加濃度が防腐剤に対して0.05〜500倍量(W/W)である請求項4または5記載の酵素の安定化組成物。
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2007055284A1 (ja) | 2005-11-11 | 2007-05-18 | Toyo Boseki Kabushiki Kaisha | 生体分子の安定化方法および組成物 |
WO2011086293A1 (fr) | 2009-12-22 | 2011-07-21 | Institut National De La Recherche Agronomique - Inra | Sulfatase modifiant selectivement les glycosaminoglycanes |
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WO2011086293A1 (fr) | 2009-12-22 | 2011-07-21 | Institut National De La Recherche Agronomique - Inra | Sulfatase modifiant selectivement les glycosaminoglycanes |
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