JP2002543980A5 - - Google Patents
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- Publication number
- JP2002543980A5 JP2002543980A5 JP2000618447A JP2000618447A JP2002543980A5 JP 2002543980 A5 JP2002543980 A5 JP 2002543980A5 JP 2000618447 A JP2000618447 A JP 2000618447A JP 2000618447 A JP2000618447 A JP 2000618447A JP 2002543980 A5 JP2002543980 A5 JP 2002543980A5
- Authority
- JP
- Japan
- Prior art keywords
- solid phase
- solution
- mixed bed
- silica
- bed solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 123
- 239000007790 solid phase Substances 0.000 description 119
- 238000000034 method Methods 0.000 description 61
- 239000000377 silicon dioxide Substances 0.000 description 50
- 239000006249 magnetic particle Substances 0.000 description 45
- 150000007523 nucleic acids Chemical class 0.000 description 43
- 102000039446 nucleic acids Human genes 0.000 description 43
- 108020004707 nucleic acids Proteins 0.000 description 43
- 239000000203 mixture Substances 0.000 description 25
- 239000012149 elution buffer Substances 0.000 description 23
- 239000013612 plasmid Substances 0.000 description 18
- 150000003839 salts Chemical class 0.000 description 18
- 238000005349 anion exchange Methods 0.000 description 15
- 239000000356 contaminant Substances 0.000 description 11
- 239000011248 coating agent Substances 0.000 description 7
- 238000000576 coating method Methods 0.000 description 7
- 239000002158 endotoxin Substances 0.000 description 7
- 229910052814 silicon oxide Inorganic materials 0.000 description 7
- ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 6
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 6
- 230000003196 chaotropic effect Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 150000002500 ions Chemical class 0.000 description 6
- 125000001664 diethylamino group Chemical group [H]C([H])([H])C([H])([H])N(*)C([H])([H])C([H])([H])[H] 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000000717 retained effect Effects 0.000 description 4
- CNMAQBJBWQQZFZ-LURJTMIESA-N (2s)-2-(pyridin-2-ylamino)propanoic acid Chemical compound OC(=O)[C@H](C)NC1=CC=CC=N1 CNMAQBJBWQQZFZ-LURJTMIESA-N 0.000 description 3
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 3
- 150000001412 amines Chemical class 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 239000006167 equilibration buffer Substances 0.000 description 3
- 229960001340 histamine Drugs 0.000 description 3
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 3
- 238000005342 ion exchange Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- SGKDAFJDYSMACD-UHFFFAOYSA-N dimethoxy(propyl)silane Chemical compound CCC[SiH](OC)OC SGKDAFJDYSMACD-UHFFFAOYSA-N 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/312,139 US6270970B1 (en) | 1999-05-14 | 1999-05-14 | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| US09/312,139 | 1999-05-14 | ||
| PCT/US2000/012954 WO2000070041A1 (en) | 1999-05-14 | 2000-05-12 | Mixed-bed solid phase and its use in the isolation of nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002543980A JP2002543980A (ja) | 2002-12-24 |
| JP2002543980A5 true JP2002543980A5 (https=) | 2006-08-17 |
Family
ID=23210049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000618447A Ceased JP2002543980A (ja) | 1999-05-14 | 2000-05-12 | 混合床固相及び核酸の単離におけるその使用 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6270970B1 (https=) |
| EP (1) | EP1179056B1 (https=) |
| JP (1) | JP2002543980A (https=) |
| AT (1) | ATE272111T1 (https=) |
| AU (1) | AU774810B2 (https=) |
| CA (1) | CA2372521C (https=) |
| DE (1) | DE60012506T2 (https=) |
| WO (1) | WO2000070041A1 (https=) |
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-
1999
- 1999-05-14 US US09/312,139 patent/US6270970B1/en not_active Expired - Fee Related
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2000
- 2000-05-12 EP EP00930626A patent/EP1179056B1/en not_active Expired - Lifetime
- 2000-05-12 WO PCT/US2000/012954 patent/WO2000070041A1/en not_active Ceased
- 2000-05-12 JP JP2000618447A patent/JP2002543980A/ja not_active Ceased
- 2000-05-12 CA CA002372521A patent/CA2372521C/en not_active Expired - Fee Related
- 2000-05-12 AU AU48415/00A patent/AU774810B2/en not_active Ceased
- 2000-05-12 DE DE60012506T patent/DE60012506T2/de not_active Expired - Lifetime
- 2000-05-12 AT AT00930626T patent/ATE272111T1/de not_active IP Right Cessation
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2001
- 2001-07-24 US US09/912,045 patent/US6376194B2/en not_active Expired - Lifetime
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