JP2002512252A5 - - Google Patents
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- Publication number
- JP2002512252A5 JP2002512252A5 JP2000544678A JP2000544678A JP2002512252A5 JP 2002512252 A5 JP2002512252 A5 JP 2002512252A5 JP 2000544678 A JP2000544678 A JP 2000544678A JP 2000544678 A JP2000544678 A JP 2000544678A JP 2002512252 A5 JP2002512252 A5 JP 2002512252A5
- Authority
- JP
- Japan
- Prior art keywords
- solution
- silica
- matrix
- nucleic acid
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 83
- 239000011159 matrix material Substances 0.000 description 40
- 239000000377 silicon dioxide Substances 0.000 description 37
- 238000000034 method Methods 0.000 description 36
- 230000003196 chaotropic effect Effects 0.000 description 16
- 239000002158 endotoxin Substances 0.000 description 16
- 108020004707 nucleic acids Proteins 0.000 description 16
- 150000007523 nucleic acids Chemical class 0.000 description 16
- 102000039446 nucleic acids Human genes 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 239000006167 equilibration buffer Substances 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 6
- 230000027455 binding Effects 0.000 description 5
- 239000011230 binding agent Substances 0.000 description 5
- 239000000741 silica gel Substances 0.000 description 5
- 229910002027 silica gel Inorganic materials 0.000 description 5
- 239000005909 Kieselgur Substances 0.000 description 4
- 239000012149 elution buffer Substances 0.000 description 4
- 239000005337 ground glass Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 3
- 239000006249 magnetic particle Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 150000003839 salts Chemical group 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- 230000004568 DNA-binding Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000005289 controlled pore glass Substances 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical group [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/064,449 | 1998-04-22 | ||
| US09/064,449 US6194562B1 (en) | 1998-04-22 | 1998-04-22 | Endotoxin reduction in nucleic acid purification |
| PCT/US1999/008491 WO1999054340A1 (en) | 1998-04-22 | 1999-04-22 | Endotoxin reduction in nucleic acid purification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002512252A JP2002512252A (ja) | 2002-04-23 |
| JP2002512252A5 true JP2002512252A5 (https=) | 2006-01-05 |
Family
ID=22056061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000544678A Pending JP2002512252A (ja) | 1998-04-22 | 1999-04-22 | 核酸の精製における内毒素の低減 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6194562B1 (https=) |
| EP (1) | EP1071695A4 (https=) |
| JP (1) | JP2002512252A (https=) |
| AU (1) | AU740145B2 (https=) |
| CA (1) | CA2329067C (https=) |
| WO (1) | WO1999054340A1 (https=) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE187733T1 (de) * | 1994-02-07 | 2000-01-15 | Qiagen Gmbh | Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie |
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| WO1999029703A2 (en) * | 1997-12-06 | 1999-06-17 | Dna Research Instruments Limited | Isolation of nucleic acids |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| EP1147226B1 (en) * | 1999-01-27 | 2013-01-23 | Folim G. Halaka | Materials and methods for the purification of polyelectrolytes |
| DE19903507A1 (de) * | 1999-01-29 | 2000-08-10 | Roche Diagnostics Gmbh | Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung |
| WO2002000342A2 (en) * | 2000-06-23 | 2002-01-03 | Irm, Llc | Method and apparatus for performing an assay |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| AU2002217784A1 (en) * | 2000-11-28 | 2002-06-11 | Promega Corporation | Purification of dna sequencing reactions using silica magnetic particles |
| KR100463415B1 (ko) * | 2001-12-12 | 2004-12-23 | 씨제이 주식회사 | 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법 |
| JP2005538929A (ja) | 2002-01-16 | 2005-12-22 | ダイナル バイオテック エイエスエイ | 単一サンプルからの核酸及びタンパク質の単離方法 |
| US7183104B1 (en) | 2002-08-23 | 2007-02-27 | Duane Morris Llp | Separator and particle detection system |
| EP2216415B2 (en) * | 2003-08-01 | 2017-01-04 | Life Technologies Corporation | Methods for preparing short RNA molecules |
| WO2005068662A1 (en) * | 2003-12-30 | 2005-07-28 | Sigma-Aldrich Co. | Rapid preparation of nucleic acids by enzymatic digestion |
| US7531308B2 (en) * | 2004-04-23 | 2009-05-12 | Sigma-Aldrich Co. | Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography |
| EP1776458A2 (en) * | 2004-07-30 | 2007-04-25 | Agencourt Bioscience Corporation | Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers |
| US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| US20070015191A1 (en) * | 2005-07-01 | 2007-01-18 | Promega Corporation | Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification |
| DE102005059217B4 (de) | 2005-12-07 | 2011-03-17 | Aj Innuscreen Gmbh | Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren |
| US8030034B2 (en) * | 2005-12-09 | 2011-10-04 | Promega Corporation | Nucleic acid purification with a binding matrix |
| CN101091797B (zh) * | 2006-06-23 | 2012-08-01 | 上海海规生物科技有限公司 | 去除初纯质粒或蛋白质溶液中内毒素的方法及试剂盒 |
| US8163535B2 (en) * | 2006-06-26 | 2012-04-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| EP2041318A2 (en) | 2006-06-26 | 2009-04-01 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| CA2739341A1 (en) * | 2008-04-30 | 2009-11-05 | Strike Bio, Inc. | Highly pure plasmid dna preparations and processes for preparing the same |
| DE102008029356A1 (de) * | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| CN102264899A (zh) * | 2008-11-04 | 2011-11-30 | 血细胞保存公司 | 弯曲的玻璃表面上的核酸提取 |
| DE102008061714A1 (de) | 2008-12-12 | 2010-06-17 | Siemens Healthcare Diagnostics Inc., Deerfield | Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe |
| US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| GB2477752A (en) * | 2010-02-11 | 2011-08-17 | Arab Biotechnology Company | Detection of bacteria |
| US11274292B2 (en) | 2011-03-29 | 2022-03-15 | Phynexus, Inc. | Devices and methods for plasmid purification |
| WO2012159063A2 (en) | 2011-05-19 | 2012-11-22 | Blood Cell Strorage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
| CA2863215C (en) | 2012-01-30 | 2021-05-04 | Exact Sciences Corporation | Modification of dna on magnetic beads |
| EP2770056B1 (en) * | 2012-07-18 | 2017-05-31 | Zymo Research Corporation | Nucleic acid purification |
| JPWO2015050191A1 (ja) * | 2013-10-03 | 2017-03-09 | 協和発酵バイオ株式会社 | 二重鎖リボ核酸の精製方法 |
| CN104370997B (zh) * | 2014-09-24 | 2018-07-31 | 陈辉 | 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法 |
| EP3906982B2 (de) | 2020-05-08 | 2025-08-13 | AXAGARIUS GmbH & Co. KG | Verfahren zur plasmidreinigung unter gleichzeitiger abreicherung von endotoxinen |
| EP4189086B1 (en) | 2020-07-29 | 2026-03-11 | Exact Sciences Corporation | Purification of sulfonated dna |
| WO2026055641A1 (en) | 2024-09-06 | 2026-03-12 | Exact Sciences Corporation | Methods and compositions for preparing biomolecule analytes from complex samples |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3897309A (en) | 1974-02-15 | 1975-07-29 | Merck & Co Inc | Process for removing pyrogenic material from aqueous solutions |
| US4059512A (en) | 1974-12-27 | 1977-11-22 | Preventive Systems, Inc. | Process for removing endotoxin from biological fluids |
| US4491660A (en) | 1980-01-10 | 1985-01-01 | Abbott Laboratories | Matrix polymers for binding endotoxins |
| US4866034A (en) * | 1982-05-26 | 1989-09-12 | Ribi Immunochem Research Inc. | Refined detoxified endotoxin |
| JPH0738880B2 (ja) | 1983-06-24 | 1995-05-01 | 東レ株式会社 | エンドトキシン血症治療剤 |
| KR940005589B1 (ko) | 1986-04-02 | 1994-06-21 | 다이닛뽄세이야꾸 가부시끼가이샤 | 핵산 또는 엔도톡신의 제거제 및 제거방법 |
| DK255887D0 (da) | 1987-05-20 | 1987-05-20 | Claus Koch | Immunoassay |
| US4808314A (en) | 1987-09-18 | 1989-02-28 | Scripps Clinic And Research Foundation | Method for reducing bacterial endotoxin contamination in solutions of macromolecules |
| US5169535A (en) | 1989-09-22 | 1992-12-08 | Kurita Water Industries Ltd. | Method of removing endotoxin |
| US5059527A (en) | 1989-12-08 | 1991-10-22 | White David C | Detection of endotoxins of gram negative bacteria |
| US5371186A (en) | 1991-02-11 | 1994-12-06 | Biosynth S.R.L. | Synthetic peptides for detoxification of bacterial endotoxins and for the prevention and treatment of septic shock |
| US5346994A (en) | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| DE69324716T2 (de) | 1992-02-13 | 1999-09-09 | Becton Dickinson And Co. | Celithydrat und Reinigung von DNS |
| DE4331358A1 (de) | 1992-10-12 | 1994-04-14 | Braun Melsungen Ag | Verfahren zur quantitativen selektiven Entfernung oder präparativen Gewinnung von Tumor-Nekrose-Faktor (TNF) oder/und Lipopolysacchariden (LPS) aus wäßrigen Flüssigkeiten |
| AU689815B2 (en) | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| US5503816A (en) | 1993-09-27 | 1996-04-02 | Becton Dickinson And Company | Silicate compounds for DNA purification |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5990301A (en) * | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| ATE187733T1 (de) * | 1994-02-07 | 2000-01-15 | Qiagen Gmbh | Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie |
| US5576185A (en) | 1994-04-15 | 1996-11-19 | Coulter Corporation | Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| JP2965131B2 (ja) * | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
| GB9604921D0 (en) * | 1996-03-08 | 1996-05-08 | Nat Blood Authority | Purification method |
| US5981235A (en) * | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
-
1998
- 1998-04-22 US US09/064,449 patent/US6194562B1/en not_active Expired - Fee Related
-
1999
- 1999-04-22 WO PCT/US1999/008491 patent/WO1999054340A1/en not_active Ceased
- 1999-04-22 JP JP2000544678A patent/JP2002512252A/ja active Pending
- 1999-04-22 AU AU36513/99A patent/AU740145B2/en not_active Ceased
- 1999-04-22 CA CA002329067A patent/CA2329067C/en not_active Expired - Fee Related
- 1999-04-22 EP EP99918650A patent/EP1071695A4/en not_active Withdrawn
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