JP2002543979A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2002543979A5 JP2002543979A5 JP2000618288A JP2000618288A JP2002543979A5 JP 2002543979 A5 JP2002543979 A5 JP 2002543979A5 JP 2000618288 A JP2000618288 A JP 2000618288A JP 2000618288 A JP2000618288 A JP 2000618288A JP 2002543979 A5 JP2002543979 A5 JP 2002543979A5
- Authority
- JP
- Japan
- Prior art keywords
- matrix
- spacer
- alkyl chain
- linker
- ion exchange
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000011159 matrix material Substances 0.000 description 78
- 238000000034 method Methods 0.000 description 47
- 238000005342 ion exchange Methods 0.000 description 41
- 150000001412 amines Chemical class 0.000 description 32
- 239000003446 ligand Substances 0.000 description 30
- 125000006850 spacer group Chemical group 0.000 description 30
- 150000007523 nucleic acids Chemical class 0.000 description 26
- 102000039446 nucleic acids Human genes 0.000 description 26
- 108020004707 nucleic acids Proteins 0.000 description 26
- 125000002029 aromatic hydrocarbon group Chemical group 0.000 description 21
- 230000002378 acidificating effect Effects 0.000 description 19
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical group NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 16
- 239000007790 solid phase Substances 0.000 description 16
- 125000005647 linker group Chemical group 0.000 description 14
- 239000007787 solid Substances 0.000 description 12
- 238000003795 desorption Methods 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 9
- 125000004432 carbon atom Chemical group C* 0.000 description 8
- 230000001419 dependent effect Effects 0.000 description 8
- 229960001340 histamine Drugs 0.000 description 8
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 8
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 6
- 150000001413 amino acids Chemical group 0.000 description 6
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 5
- 235000004279 alanine Nutrition 0.000 description 5
- 239000004202 carbamide Substances 0.000 description 5
- 235000018417 cysteine Nutrition 0.000 description 5
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 5
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 4
- 125000000217 alkyl group Chemical group 0.000 description 4
- 150000001450 anions Chemical group 0.000 description 4
- 150000004982 aromatic amines Chemical class 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 4
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 4
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 125000006239 protecting group Chemical group 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 150000003141 primary amines Chemical class 0.000 description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 3
- 150000003335 secondary amines Chemical class 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 125000001424 substituent group Chemical group 0.000 description 3
- 150000003512 tertiary amines Chemical class 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 150000002118 epoxides Chemical class 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical group O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- CNMAQBJBWQQZFZ-LURJTMIESA-N (2s)-2-(pyridin-2-ylamino)propanoic acid Chemical compound OC(=O)[C@H](C)NC1=CC=CC=N1 CNMAQBJBWQQZFZ-LURJTMIESA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001735 carboxylic acids Chemical group 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- IYMSIPPWHNIMGE-UHFFFAOYSA-N silylurea Chemical compound NC(=O)N[SiH3] IYMSIPPWHNIMGE-UHFFFAOYSA-N 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/312,172 | 1999-05-14 | ||
| US09/312,172 US6310199B1 (en) | 1999-05-14 | 1999-05-14 | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| PCT/US2000/012186 WO2000069872A2 (en) | 1999-05-14 | 2000-05-05 | pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002543979A JP2002543979A (ja) | 2002-12-24 |
| JP2002543979A5 true JP2002543979A5 (https=) | 2006-08-03 |
| JP4377550B2 JP4377550B2 (ja) | 2009-12-02 |
Family
ID=23210195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000618288A Expired - Fee Related JP4377550B2 (ja) | 1999-05-14 | 2000-05-05 | ｐＨ依存型イオン交換マトリックス及び核酸の単離におけるその使用法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6310199B1 (https=) |
| EP (1) | EP1179057B1 (https=) |
| JP (1) | JP4377550B2 (https=) |
| AT (1) | ATE271605T1 (https=) |
| AU (1) | AU772046B2 (https=) |
| CA (1) | CA2372054A1 (https=) |
| DE (1) | DE60012318T2 (https=) |
| WO (1) | WO2000069872A2 (https=) |
Families Citing this family (127)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| WO1999029703A2 (en) * | 1997-12-06 | 1999-06-17 | Dna Research Instruments Limited | Isolation of nucleic acids |
| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| DE60123828T2 (de) * | 2000-11-13 | 2007-05-16 | Promega Corp., Madison | Lysat-klärung und nukleinsäureisolierung mit silanisierten silicamatrizes |
| SE0004932D0 (sv) * | 2000-12-31 | 2000-12-31 | Apbiotech Ab | A method for mixed mode adsorption and mixed mode adsorbents |
| CA2431662A1 (en) * | 2000-12-31 | 2002-07-11 | Amersham Biosciences Ab | A method for the manufacture of compositions containing low concentrations of salts |
| US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
| US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
| JP2005538929A (ja) * | 2002-01-16 | 2005-12-22 | ダイナル バイオテック エイエスエイ | 単一サンプルからの核酸及びタンパク質の単離方法 |
| AU2003217552A1 (en) * | 2002-02-19 | 2003-09-09 | Choicepoint Asset Company | Selective extraction of dna from groups of cells |
| CN1643145A (zh) * | 2002-03-21 | 2005-07-20 | 艾夫西亚生物科技公司 | 寡核苷酸及其类似物的纯化方法 |
| US20060035224A1 (en) * | 2002-03-21 | 2006-02-16 | Johansen Jack T | Purification methods for oligonucleotides and their analogs |
| US7052840B2 (en) * | 2002-04-03 | 2006-05-30 | Capitol Genomix, Inc. | Reversible association of nucleic acid with a carboxylated substrate |
| GB0210766D0 (en) * | 2002-05-10 | 2002-06-19 | Genovision As | Isolating nucleic acid |
| US7597936B2 (en) * | 2002-11-26 | 2009-10-06 | University Of Utah Research Foundation | Method of producing a pigmented composite microporous material |
| US7682688B2 (en) * | 2002-11-26 | 2010-03-23 | University Of Utah Research Foundation | Microporous materials, methods, and articles for localizing and quantifying analytes |
| GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
| US20050042660A1 (en) * | 2003-07-31 | 2005-02-24 | Hall Gerald Edward | Devices and methods for isolating RNA |
| US20050026153A1 (en) * | 2003-07-31 | 2005-02-03 | Iannotti Claudia A. | Devices and methods for isolating RNA |
| EP3718635A1 (en) | 2003-07-31 | 2020-10-07 | Handylab, Inc. | Processing particle-containing samples |
| DE10340746A1 (de) * | 2003-09-01 | 2005-03-31 | Decoma (Germany) Gmbh | Dachboxeinrichtung, und Dachmodul mit integrierter Dachboxeinrichtung |
| EP1529840A1 (en) * | 2003-11-04 | 2005-05-11 | Qiagen GmbH | A rapid and low cost method for isolating nucleic acid |
| DE10355409A1 (de) * | 2003-11-25 | 2005-06-30 | Magnamedics Gmbh | Sphärische, magnetische Silicagel-Träger mit vergrößerter Oberfläche für die Aufreinigung von Nukleinsäuren |
| US20060160122A1 (en) * | 2004-02-18 | 2006-07-20 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| US20050181378A1 (en) * | 2004-02-18 | 2005-08-18 | Applera Corporation | Polyelectrolyte-coated size-exclusion ion-exchange particles |
| SE0400490D0 (sv) * | 2004-02-26 | 2004-02-26 | Amersham Biosciences Ab | Plasmid purification |
| ES2553097T3 (es) * | 2004-05-03 | 2015-12-04 | Handylab, Inc. | Procesamiento de muestras que contienen polinucleótidos |
| US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
| DE102004023586A1 (de) * | 2004-05-13 | 2005-12-08 | Universität Bremen | Protonenleitfähiges, vernetztes Heteropolysiloxan, protonenleitfähige Membran und Verfahren zu deren Herstellung |
| US20060099605A1 (en) * | 2004-11-11 | 2006-05-11 | Hall Gerald E Jr | Devices and methods for isolating RNA |
| US7402243B2 (en) * | 2004-09-10 | 2008-07-22 | Dionex Corporation | Organosilanes and substrate bonded with same |
| EP1693108A1 (en) * | 2004-12-04 | 2006-08-23 | MERCK PATENT GmbH | Mixed-modal anion-exchange type separation material |
| KR100647306B1 (ko) * | 2004-12-23 | 2006-11-23 | 삼성전자주식회사 | 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 |
| US7964380B2 (en) * | 2005-01-21 | 2011-06-21 | Argylia Technologies | Nanoparticles for manipulation of biopolymers and methods of thereof |
| KR100647315B1 (ko) * | 2005-02-02 | 2006-11-23 | 삼성전자주식회사 | 실란화된 고상 물질을 이용한 핵산의 분리 및 증폭 방법 |
| US7468130B2 (en) * | 2005-02-15 | 2008-12-23 | Dionex Corporation | Organosilanes and substrates covalently bonded with same and methods for synthesis and use same |
| US20060223072A1 (en) * | 2005-03-31 | 2006-10-05 | Boyes Barry E | Methods of using a DNase I-like enzyme |
| US20060223073A1 (en) * | 2005-03-31 | 2006-10-05 | Boyes Barry E | Methods of using a DNase I-like enzyme |
| KR100657957B1 (ko) * | 2005-04-12 | 2006-12-14 | 삼성전자주식회사 | 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 및 상기방법에 사용될 수 있는 핵산 분리용 고상 물질 |
| US20060234251A1 (en) * | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
| ATE551433T1 (de) | 2005-05-06 | 2012-04-15 | Gen Probe Inc | Verfahren und produkte zum erfassen von nukleinsäurezielen |
| KR100668338B1 (ko) | 2005-05-21 | 2007-01-12 | 삼성전자주식회사 | 신규한 ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는고체 기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법 |
| KR100668337B1 (ko) | 2005-05-21 | 2007-01-12 | 삼성전자주식회사 | 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법 |
| US20060270843A1 (en) * | 2005-05-26 | 2006-11-30 | Hall Gerald E Jr | Methods for isolation of nucleic acids |
| US20070015191A1 (en) * | 2005-07-01 | 2007-01-18 | Promega Corporation | Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification |
| CA2617645C (en) | 2005-08-03 | 2014-09-09 | Merck Patent Gesellschaft Mit Beschraenkter Haftung | Hydrophilic crosslinked polymer |
| EP1754534A1 (de) | 2005-08-03 | 2007-02-21 | MERCK PATENT GmbH | Hydrophiles vernetztes Polymer |
| US8030034B2 (en) * | 2005-12-09 | 2011-10-04 | Promega Corporation | Nucleic acid purification with a binding matrix |
| KR100829574B1 (ko) * | 2006-01-03 | 2008-05-14 | 삼성전자주식회사 | 마이크로어레이용 기판, 그 마이크로어레이용 기판을이용하여 생분자를 분석하는 방법, 및 그 마이크로어레이용기판을 포함하는 랩온어칩 |
| US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
| ES2692380T3 (es) | 2006-03-24 | 2018-12-03 | Handylab, Inc. | Método para realizar PCR con un cartucho con varias pistas |
| US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| JP4331256B2 (ja) * | 2006-04-12 | 2009-09-16 | パナソニック株式会社 | 有機分子膜構造体の形成方法 |
| US20080113357A1 (en) * | 2006-06-29 | 2008-05-15 | Millipore Corporation | Filter device for the isolation of a nucleic acid |
| US20080026375A1 (en) * | 2006-07-31 | 2008-01-31 | Sigma Aldrich Co. | Compositions and Methods for Isolation of Biological Molecules |
| US20080026374A1 (en) * | 2006-07-31 | 2008-01-31 | Sigma Aldrich Co. | Compositions and Methods for Isolation of Biological Molecules |
| US20080023395A1 (en) * | 2006-07-31 | 2008-01-31 | Sigma Aldrich Co. | Compositions and Methods for Isolation of Biological Molecules |
| GB2443505B (en) * | 2006-09-26 | 2008-12-31 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2445441B (en) * | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2445442A (en) * | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
| US7492312B2 (en) * | 2006-11-14 | 2009-02-17 | Fam Adly T | Multiplicative mismatched filters for optimum range sidelobe suppression in barker code reception |
| WO2008060604A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US8765076B2 (en) | 2006-11-14 | 2014-07-01 | Handylab, Inc. | Microfluidic valve and method of making same |
| CA2629589C (en) | 2007-04-20 | 2016-03-29 | F.Hoffmann-La Roche Ag | Isolation and purification of nucleic acid molecules with a solid phase |
| US7557232B2 (en) * | 2007-05-25 | 2009-07-07 | Dionex Corporation | Compositions useful as chromatography stationary phases |
| US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
| US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
| US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
| US9618139B2 (en) | 2007-07-13 | 2017-04-11 | Handylab, Inc. | Integrated heater and magnetic separator |
| AU2008276211B2 (en) | 2007-07-13 | 2015-01-22 | Handylab, Inc. | Polynucleotide capture materials, and methods of using same |
| US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| US8569221B2 (en) | 2007-08-30 | 2013-10-29 | Kimberly-Clark Worldwide, Inc. | Stain-discharging and removing system |
| US8067580B2 (en) * | 2008-02-07 | 2011-11-29 | Ge Healthcare Bio-Sciences Corp. | Isolation of DNA, RNA and protein from a single sample |
| US8188242B2 (en) | 2008-04-08 | 2012-05-29 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| GB0814570D0 (en) * | 2008-08-08 | 2008-09-17 | Diagnostics For The Real World | Isolation of nucleic acid |
| KR101015502B1 (ko) * | 2008-09-09 | 2011-02-22 | 삼성전자주식회사 | 제1 ｐＨ에서 양전하 및 제2 ｐＨ에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법 |
| KR20100070994A (ko) * | 2008-12-18 | 2010-06-28 | 토소가부시키가이샤 | 액체 크로마토그래피용 충전 재료 및 해당 충전 재료에 의한 생체고분자의 분리·정제 방법 |
| DE102008063003A1 (de) * | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| DE102008063001A1 (de) * | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| EP2256195A1 (de) * | 2009-05-12 | 2010-12-01 | Qiagen GmbH | Nukleinsäureaufreinigungsverfahren |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| US8222397B2 (en) | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| WO2011046494A1 (en) * | 2009-10-12 | 2011-04-21 | Ge Healthcare Bio-Sciences Ab | Separation matrices |
| WO2011081869A2 (en) * | 2009-12-14 | 2011-07-07 | Betty Wu | Method and materials for separating nucleic acid materials |
| MX2012006983A (es) * | 2009-12-17 | 2013-02-06 | Instraction Gmbh | Absorbente especifico para unir proteinas y peptidos, y metodo de separacion que utiliza el mismo. |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US11031095B2 (en) | 2010-08-06 | 2021-06-08 | Ariosa Diagnostics, Inc. | Assay systems for determination of fetal copy number variation |
| US20140342940A1 (en) | 2011-01-25 | 2014-11-20 | Ariosa Diagnostics, Inc. | Detection of Target Nucleic Acids using Hybridization |
| US8700338B2 (en) | 2011-01-25 | 2014-04-15 | Ariosa Diagnosis, Inc. | Risk calculation for evaluation of fetal aneuploidy |
| US20130040375A1 (en) | 2011-08-08 | 2013-02-14 | Tandem Diagnotics, Inc. | Assay systems for genetic analysis |
| US10533223B2 (en) | 2010-08-06 | 2020-01-14 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US10167508B2 (en) | 2010-08-06 | 2019-01-01 | Ariosa Diagnostics, Inc. | Detection of genetic abnormalities |
| US20130261003A1 (en) | 2010-08-06 | 2013-10-03 | Ariosa Diagnostics, In. | Ligation-based detection of genetic variants |
| US11203786B2 (en) | 2010-08-06 | 2021-12-21 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US11270781B2 (en) | 2011-01-25 | 2022-03-08 | Ariosa Diagnostics, Inc. | Statistical analysis for non-invasive sex chromosome aneuploidy determination |
| US9994897B2 (en) | 2013-03-08 | 2018-06-12 | Ariosa Diagnostics, Inc. | Non-invasive fetal sex determination |
| US10131947B2 (en) | 2011-01-25 | 2018-11-20 | Ariosa Diagnostics, Inc. | Noninvasive detection of fetal aneuploidy in egg donor pregnancies |
| US8756020B2 (en) | 2011-01-25 | 2014-06-17 | Ariosa Diagnostics, Inc. | Enhanced risk probabilities using biomolecule estimations |
| JP6088487B2 (ja) | 2011-04-15 | 2017-03-01 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | 走査リアルタイムマイクロ流体熱サイクラーと同期熱サイクリング及び走査光学検出の方法 |
| CN102250876B (zh) | 2011-05-18 | 2014-08-13 | 李学敬 | 从生物材料中分离纯化rna的方法 |
| WO2013012545A1 (en) | 2011-07-20 | 2013-01-24 | Conocophillips Company | Surfactant system to increase hydrocarbon recovery |
| US8712697B2 (en) | 2011-09-07 | 2014-04-29 | Ariosa Diagnostics, Inc. | Determination of copy number variations using binomial probability calculations |
| JP5899731B2 (ja) | 2011-09-13 | 2016-04-06 | ソニー株式会社 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
| RU2622432C2 (ru) | 2011-09-30 | 2017-06-15 | Бектон, Дикинсон Энд Компани | Унифицированная полоска для реактивов |
| WO2013067202A1 (en) | 2011-11-04 | 2013-05-10 | Handylab, Inc. | Polynucleotide sample preparation device |
| AU2013214849B2 (en) | 2012-02-03 | 2016-09-01 | Becton, Dickinson And Company | External files for distribution of molecular diagnostic tests and determination of compatibility between tests |
| US10289800B2 (en) | 2012-05-21 | 2019-05-14 | Ariosa Diagnostics, Inc. | Processes for calculating phased fetal genomic sequences |
| JP2015522293A (ja) | 2012-07-19 | 2015-08-06 | アリオサ ダイアグノスティックス インコーポレイテッドＡｒｉｏｓａ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． | 多重化連続ライゲーションに基づく遺伝子変異体の検出 |
| RU2015114330A (ru) | 2012-09-17 | 2016-11-10 | У.Р. Грейс Энд Ко.-Конн. | Хроматографические среды и устройства |
| JP6914189B2 (ja) | 2014-05-02 | 2021-08-04 | ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ | 官能化担体材料並びに官能化担体材料を作製及び使用する方法 |
| WO2016057891A1 (en) | 2014-10-09 | 2016-04-14 | Promega Corporation | Method of isolating nucleic acid |
| WO2016096590A1 (en) * | 2014-12-16 | 2016-06-23 | Unilever Plc | Synthetic enzyme mimic based on a histidine scaffold |
| CN107921407B (zh) | 2015-06-05 | 2022-07-05 | 格雷斯公司 | 生物处理吸附澄清剂及其制备和使用方法 |
| FR3038616B1 (fr) * | 2015-07-06 | 2020-11-06 | Gl Biocontrol | Procede de purification et de concentration d'acides nucleiques. |
| US20170298413A1 (en) * | 2016-04-13 | 2017-10-19 | New York Genome Center | Methods for the isolation of biomolecules and uses thereof |
| WO2017184436A1 (en) * | 2016-04-17 | 2017-10-26 | Children's Medical Center Corporation | Systems and methods for steroidal gels |
| CN108097336B (zh) * | 2017-10-25 | 2019-02-26 | 重庆博蓝鹰生物技术有限公司 | 两性解离离子交换介质、应用方法和分离容量标定方法 |
| EP4025336A4 (en) * | 2019-09-05 | 2024-01-17 | Bio-Rad Laboratories, Inc. | ANION EXCHANGE/HYDROPHOBIC RESINS FOR MIXED MODE CHROMATOGRAPHY |
| WO2021122846A1 (en) * | 2019-12-16 | 2021-06-24 | Qiagen Gmbh | Enrichment method |
| JP7723657B2 (ja) * | 2020-05-25 | 2025-08-14 | 積水メディカル株式会社 | 核酸の精製方法 |
| KR102909544B1 (ko) * | 2020-06-17 | 2026-01-09 | 엔테그리스, 아이엔씨. | 이온-교환 멤브레인, 필터 및 방법 |
| KR102412078B1 (ko) * | 2021-12-23 | 2022-06-22 | 홍승범 | 다공성 이온 전하 결정체를 이용한 핵산 추출방법 |
| CN121079137A (zh) * | 2023-05-05 | 2025-12-05 | 生物辐射实验室股份有限公司 | 含有阴离子型和阳离子型配体的多模式色谱树脂 |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4233169A (en) | 1979-04-13 | 1980-11-11 | Corning Glass Works | Porous magnetic glass structure |
| US4297337A (en) | 1979-04-13 | 1981-10-27 | Corning Glass Works | Solid-phase immunoassays using magnetic glass |
| US4395271A (en) | 1979-04-13 | 1983-07-26 | Corning Glass Works | Method for making porous magnetic glass and crystal-containing structures |
| US4298500A (en) | 1980-05-05 | 1981-11-03 | Varian Associates, Inc. | Mixed phase chromatographic compositions |
| DE3211309A1 (de) | 1982-03-26 | 1983-09-29 | Metin Dipl.-Ing. 6100 Darmstadt Colpan | Chromatographisches verfahren zur isolierung von makromolekuelen |
| US4672040A (en) | 1983-05-12 | 1987-06-09 | Advanced Magnetics, Inc. | Magnetic particles for use in separations |
| US4695393A (en) | 1983-05-12 | 1987-09-22 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
| US4523996A (en) | 1984-02-22 | 1985-06-18 | Westinghouse Electric Corp. | Method of separating cationic from anionic beads in mixed resin beds |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5076950A (en) | 1985-12-20 | 1991-12-31 | Syntex (U.S.A.) Inc. | Magnetic composition for particle separation |
| DE3639949A1 (de) | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | Verfahren zur trennung von langkettigen nukleinsaeuren |
| US4767670A (en) | 1987-01-21 | 1988-08-30 | E. I. Du Pont De Nemours And Company | Chromatographic supports for separation of oligonucleotides |
| US5075430A (en) | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5523231A (en) | 1990-02-13 | 1996-06-04 | Amersham International Plc | Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules |
| EP0620761A1 (en) | 1991-01-04 | 1994-10-26 | Perseptive Biosystems, Inc. | Sulfonamide bonded hydrophilic coatings |
| US5155018A (en) | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5395498A (en) | 1991-11-06 | 1995-03-07 | Gombinsky; Moshe | Method for separating biological macromolecules and means therfor |
| US5734020A (en) | 1991-11-20 | 1998-03-31 | Cpg, Inc. | Production and use of magnetic porous inorganic materials |
| US5610274A (en) | 1991-11-20 | 1997-03-11 | Cpg, Inc. | Production and use of magnetic porous inorganic materials |
| US5346994A (en) | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| US5316680A (en) | 1992-10-21 | 1994-05-31 | Cornell Research Foundation, Inc. | Multimodal chromatographic separation media and process for using same |
| AT398973B (de) | 1992-11-18 | 1995-02-27 | Bonn Guenther Dr | Verfahren zur trennung von nukleinsäuren |
| WO1994018156A1 (en) | 1993-02-01 | 1994-08-18 | University Of Iowa Research Foundation | Quartenary amine surfactants and methods of using same in isolation of rna |
| DE4307262A1 (de) | 1993-03-02 | 1994-09-08 | Christian Bergemann | Magnetisches polymeres Siliciumdioxid |
| AU689815B2 (en) | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| ATE187733T1 (de) | 1994-02-07 | 2000-01-15 | Qiagen Gmbh | Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie |
| US5990301A (en) | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| US5582988A (en) | 1994-09-15 | 1996-12-10 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5652348A (en) | 1994-09-23 | 1997-07-29 | Massey University | Chromatographic resins and methods for using same |
| US5654141A (en) | 1994-11-18 | 1997-08-05 | Thomas Jefferson University | Amplification based detection of bacterial infection |
| US5660984A (en) | 1994-12-09 | 1997-08-26 | Davis; Thomas E. | DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof |
| AR003122A1 (es) | 1995-05-19 | 1998-07-08 | Merck & Co Inc | Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso. |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| JP2965131B2 (ja) | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
| US5783686A (en) | 1995-09-15 | 1998-07-21 | Beckman Instruments, Inc. | Method for purifying nucleic acids from heterogenous mixtures |
| SE9600590D0 (sv) | 1996-02-19 | 1996-02-19 | Pharmacia Biotech Ab | Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris |
| JPH09327291A (ja) | 1996-06-11 | 1997-12-22 | Toyobo Co Ltd | Ｒｎａの抽出精製方法 |
| JP3940935B2 (ja) | 1996-06-11 | 2007-07-04 | 東洋紡績株式会社 | プラスミドｄｎａの抽出精製方法 |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| PL191422B1 (pl) | 1997-01-21 | 2006-05-31 | Grace W R & Co | Adsorbent o cząstkach stałych i sposób wytwarzania cząstek adsorbenta |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
-
1999
- 1999-05-14 US US09/312,172 patent/US6310199B1/en not_active Expired - Lifetime
-
2000
- 2000-05-05 DE DE60012318T patent/DE60012318T2/de not_active Expired - Lifetime
- 2000-05-05 AT AT00935865T patent/ATE271605T1/de not_active IP Right Cessation
- 2000-05-05 CA CA002372054A patent/CA2372054A1/en not_active Abandoned
- 2000-05-05 WO PCT/US2000/012186 patent/WO2000069872A2/en not_active Ceased
- 2000-05-05 JP JP2000618288A patent/JP4377550B2/ja not_active Expired - Fee Related
- 2000-05-05 EP EP00935865A patent/EP1179057B1/en not_active Expired - Lifetime
- 2000-05-05 AU AU51261/00A patent/AU772046B2/en not_active Ceased
-
2001
- 2001-03-20 US US09/813,077 patent/US6806362B2/en not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2002543979A5 (https=) | ||
| JP2002543980A5 (https=) | ||
| JP4377550B2 (ja) | ｐＨ依存型イオン交換マトリックス及び核酸の単離におけるその使用法 | |
| Hemmati et al. | Magnetic nanoparticle based solid-phase extraction of heavy metal ions: a review on recent advances | |
| CA2723536C (en) | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control | |
| Telepchak | Forensic and clinical applications of solid phase extraction | |
| WO2011012019A1 (zh) | 基于硅胶表面共聚反应的分离材料及其制备方法 | |
| JP2004532975A (ja) | 表面から有機基を取り除いた多孔質混成物粒子 | |
| KR102226564B1 (ko) | 작용화된 미립자 지지체 물질 및 이의 제조 및 사용 방법 | |
| CN102939140A (zh) | 具有芳香族堆积特性的官能化的基质 | |
| JPS60501209A (ja) | 生物学的活性物質の精製方法 | |
| JP2007525222A5 (https=) | ||
| CA2444259A1 (en) | Isolation of dna molecules using mercapto-aryl ligands | |
| WO2000070040A1 (en) | Cell concentration and lysate clearance using paramagnetic particles | |
| CN115975643A (zh) | 一种重金属污染土壤修复材料制备及修复方法 | |
| CN108568285B (zh) | 一种磁性除砷固体螯合剂的制备方法 | |
| JPS61500078A (ja) | 添加物を含む改良シリカベ−スクロマトグラフィ−支持体 | |
| US20060076296A1 (en) | Novel stationary phases for use in high-performance liquid chromatography | |
| JP2001524133A (ja) | ポリヌクレオチド分離を実施するための改良された方法 | |
| CN109806841A (zh) | 碱性氨基酸改性硅胶材料及其制备方法和应用 | |
| EP1341910A1 (en) | Lysate clearance and nucleic acid isolation using silanized silica matrices | |
| Tsai et al. | Influence of surface hydrophobic groups on the adsorption of proteins onto nonporous polymeric particles with immobilized metal ions | |
| GB2437953A (en) | Silica-based chromatographic stationary phase | |
| CN114787194A (zh) | 使用核-壳-壳磁珠的设备系统和方法 | |
| CN109569546A (zh) | 一种用于组氨酸标签蛋白纯化的螯合金属磁性纳米颗粒的制备方法及应用 |