JP2007525222A5 - - Google Patents
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- Publication number
- JP2007525222A5 JP2007525222A5 JP2007500711A JP2007500711A JP2007525222A5 JP 2007525222 A5 JP2007525222 A5 JP 2007525222A5 JP 2007500711 A JP2007500711 A JP 2007500711A JP 2007500711 A JP2007500711 A JP 2007500711A JP 2007525222 A5 JP2007525222 A5 JP 2007525222A5
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- separation matrix
- liquid
- eluate
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 14
- 239000013612 plasmid Substances 0.000 claims 13
- 239000011159 matrix material Substances 0.000 claims 11
- 238000000926 separation method Methods 0.000 claims 9
- 239000007788 liquid Substances 0.000 claims 5
- 239000011148 porous material Substances 0.000 claims 5
- 238000005349 anion exchange Methods 0.000 claims 3
- 239000003446 ligand Substances 0.000 claims 3
- 239000000969 carrier Substances 0.000 claims 2
- 230000007717 exclusion Effects 0.000 claims 2
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 2
- 125000003277 amino group Chemical group 0.000 claims 1
- 125000001664 diethylamino group Chemical group [H]C([H])([H])C([H])([H])N(*)C([H])([H])C([H])([H])[H] 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 239000012798 spherical particle Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0400490-9 | 2004-02-26 | ||
| SE0400490A SE0400490D0 (sv) | 2004-02-26 | 2004-02-26 | Plasmid purification |
| PCT/SE2005/000229 WO2005083080A1 (en) | 2004-02-26 | 2005-02-21 | Plasmid purification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2007525222A JP2007525222A (ja) | 2007-09-06 |
| JP2007525222A5 true JP2007525222A5 (https=) | 2008-04-10 |
| JP5095386B2 JP5095386B2 (ja) | 2012-12-12 |
Family
ID=32067270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007500711A Expired - Fee Related JP5095386B2 (ja) | 2004-02-26 | 2005-02-21 | プラスミド精製 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8093373B2 (https=) |
| EP (1) | EP1718744B1 (https=) |
| JP (1) | JP5095386B2 (https=) |
| CN (1) | CN1922319A (https=) |
| SE (1) | SE0400490D0 (https=) |
| WO (1) | WO2005083080A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5793726B2 (ja) * | 2007-05-23 | 2015-10-14 | ブイジーエックスアイ，インコーポレイティド | 高濃度の生物学的に活性な分子を含む組成物とその製造方法 |
| EP2315839A4 (en) * | 2008-08-25 | 2012-03-21 | Ge Healthcare Bio Sciences | EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA |
| CN105911183B (zh) * | 2011-02-10 | 2019-03-22 | 积水医疗株式会社 | 离子交换色谱法用填充剂以及核酸链的分离检测方法 |
| US9960620B2 (en) | 2014-09-16 | 2018-05-01 | Navitas Semiconductor, Inc. | Bootstrap capacitor charging circuit for GaN devices |
| KR101751700B1 (ko) | 2014-09-18 | 2017-06-30 | 한국과학기술연구원 | 전하를 띄는 다공성 구조체 |
| WO2016192012A1 (zh) * | 2015-06-01 | 2016-12-08 | 易初丽 | 一种质粒纯化系统 |
| CN106884011B (zh) * | 2015-12-16 | 2020-11-17 | 固安鼎泰海规生物科技有限公司 | 一种规模化质粒纯化的联合液相层析分离方法 |
| US10814305B2 (en) | 2016-09-29 | 2020-10-27 | Bio-Rad Laboratories, Inc. | Agarose-filled ceramic apatite |
| EP4234710A3 (en) | 2017-03-28 | 2023-11-01 | Phase Scientific International, Ltd. | Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy |
| SG11201909324WA (en) | 2017-06-01 | 2019-11-28 | Yin To Chiu | Phase separation behavior modifying agents for aqueous two-phase separation within porous material |
| WO2019046563A1 (en) | 2017-09-01 | 2019-03-07 | Phase Diagnostics, Inc. | METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS |
| ES2964100T3 (es) | 2017-09-18 | 2024-04-04 | Phase Scient International Ltd | Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos |
| US11479765B2 (en) | 2018-01-19 | 2022-10-25 | Phase Scientific International, Ltd. | Method of isolating exosomes using encapsulation and aqueous micellar system |
| WO2019143895A1 (en) | 2018-01-19 | 2019-07-25 | Yin To Chiu | Spontaneous nucleic acid purification and concentration in a single step |
| EP3740588A4 (en) * | 2018-01-19 | 2021-10-20 | Phase Scientific International, Ltd. | METHOD OF ISOLATION AND PURIFICATION OF NUCLEIC ACIDS USING A SOLID-LIQUID PHASE SYSTEM |
| CN111088248B (zh) * | 2019-12-23 | 2020-10-16 | 广州湾区生物科技有限公司 | 一种用于提取无内毒素质粒的磁性材料及其使用方法 |
| EP4106897A4 (en) | 2020-02-19 | 2023-10-25 | Bio-Rad Laboratories, Inc. | Method of preparing polymer-filled chromatography resin |
| EP4121530A1 (en) | 2020-03-20 | 2023-01-25 | Phase Scientific International, Ltd. | Compositions and methods for ribonucleic acid extraction |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE9601368D0 (sv) | 1996-04-11 | 1996-04-11 | Pharmacia Biotech Ab | Process for the production of a porous cross-linked polysaccharide gel |
| US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US6011148A (en) | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| US6498236B1 (en) | 1996-08-30 | 2002-12-24 | Upfront Chromatography A/S | Isolation of immunoglobulins |
| SE9700769D0 (sv) | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
| US6214586B1 (en) | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| US6441160B2 (en) | 1998-05-11 | 2002-08-27 | Tosoh Corporation | Separating plasmids from contaminants using hydrophobic or hydrophobic and ion exchange chromatography |
| AU4314099A (en) * | 1998-06-01 | 1999-12-20 | Immune Response Corporation, The | Novel method of large scale plasmid purification |
| EP0987327A1 (en) | 1998-09-14 | 2000-03-22 | QIAGEN GmbH | Novel method for purifying covalently closed circular DNA |
| US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| CA2378944A1 (en) | 1999-07-23 | 2001-02-01 | Genentech, Inc. | Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration |
| SE9904272D0 (sv) * | 1999-11-25 | 1999-11-25 | Amersham Pharm Biotech Ab | A method for selective removal of a substance from samples containing compounds having nucleic acid structure |
| DE10009982A1 (de) * | 2000-03-03 | 2001-09-06 | Qiagen Gmbh | Polymere Anionenaustauscher und deren Verwendung in chromatographischen Verfahren |
| EP1525053A1 (en) * | 2002-07-26 | 2005-04-27 | Applera Corporation | Device and method for purification of nucleic acids |
-
2004
- 2004-02-26 SE SE0400490A patent/SE0400490D0/xx unknown
-
2005
- 2005-02-21 JP JP2007500711A patent/JP5095386B2/ja not_active Expired - Fee Related
- 2005-02-21 EP EP05711089.2A patent/EP1718744B1/en not_active Expired - Lifetime
- 2005-02-21 CN CNA2005800059786A patent/CN1922319A/zh active Pending
- 2005-02-21 US US10/589,740 patent/US8093373B2/en active Active
- 2005-02-21 WO PCT/SE2005/000229 patent/WO2005083080A1/en not_active Ceased
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