CN1922319A - 质粒纯化 - Google Patents

质粒纯化 Download PDF

Info

Publication number
CN1922319A
CN1922319A CNA2005800059786A CN200580005978A CN1922319A CN 1922319 A CN1922319 A CN 1922319A CN A2005800059786 A CNA2005800059786 A CN A2005800059786A CN 200580005978 A CN200580005978 A CN 200580005978A CN 1922319 A CN1922319 A CN 1922319A
Authority
CN
China
Prior art keywords
plasmid
matrix
separation matrix
plasmids
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2005800059786A
Other languages
English (en)
Chinese (zh)
Inventor
J·范阿尔斯泰恩
J·贝里斯特伦
D·林德斯特伦
J·施塔德勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of CN1922319A publication Critical patent/CN1922319A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
CNA2005800059786A 2004-02-26 2005-02-21 质粒纯化 Pending CN1922319A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0400490A SE0400490D0 (sv) 2004-02-26 2004-02-26 Plasmid purification
SE04004909 2004-02-26

Publications (1)

Publication Number Publication Date
CN1922319A true CN1922319A (zh) 2007-02-28

Family

ID=32067270

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2005800059786A Pending CN1922319A (zh) 2004-02-26 2005-02-21 质粒纯化

Country Status (6)

Country Link
US (1) US8093373B2 (https=)
EP (1) EP1718744B1 (https=)
JP (1) JP5095386B2 (https=)
CN (1) CN1922319A (https=)
SE (1) SE0400490D0 (https=)
WO (1) WO2005083080A1 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105567672A (zh) * 2007-05-23 2016-05-11 Vgxi公司 包含高浓度的生物活性分子的组合物及其制备工艺
WO2016192012A1 (zh) * 2015-06-01 2016-12-08 易初丽 一种质粒纯化系统
CN106884011A (zh) * 2015-12-16 2017-06-23 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111088248A (zh) * 2019-12-23 2020-05-01 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2315839A4 (en) * 2008-08-25 2012-03-21 Ge Healthcare Bio Sciences EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA
EP2674753B1 (en) * 2011-02-10 2020-05-13 Sekisui Medical Co., Ltd. Method for separating and detecting a nucleic acid strand
US9960620B2 (en) 2014-09-16 2018-05-01 Navitas Semiconductor, Inc. Bootstrap capacitor charging circuit for GaN devices
KR101751700B1 (ko) 2014-09-18 2017-06-30 한국과학기술연구원 전하를 띄는 다공성 구조체
US10814305B2 (en) 2016-09-29 2020-10-27 Bio-Rad Laboratories, Inc. Agarose-filled ceramic apatite
WO2018183465A1 (en) 2017-03-28 2018-10-04 Chiu Yin To Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy
CN119425158A (zh) 2017-06-01 2025-02-14 相达生物科技美国有限公司 用于多孔材料中双水相分离的相分离行为改性剂
WO2019046563A1 (en) 2017-09-01 2019-03-07 Phase Diagnostics, Inc. METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS
EP4303311A3 (en) 2017-09-18 2024-04-03 Phase Scientific International, Ltd. Method for using aqueous two-phase system for the isolation, purification and/or concentration of short nucleic acid fragments
US11479765B2 (en) 2018-01-19 2022-10-25 Phase Scientific International, Ltd. Method of isolating exosomes using encapsulation and aqueous micellar system
US12043826B2 (en) 2018-01-19 2024-07-23 Phase Scientific International, Ltd. Spontaneous nucleic acid purification and concentration in a single step
CN111867721A (zh) * 2018-01-19 2020-10-30 相达生物科技国际有限公司 使用固-液相系统分离和纯化核酸的方法
EP4106897A4 (en) 2020-02-19 2023-10-25 Bio-Rad Laboratories, Inc. Method of preparing polymer-filled chromatography resin
EP4121530A1 (en) 2020-03-20 2023-01-25 Phase Scientific International, Ltd. Compositions and methods for ribonucleic acid extraction

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9601368D0 (sv) 1996-04-11 1996-04-11 Pharmacia Biotech Ab Process for the production of a porous cross-linked polysaccharide gel
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6011148A (en) 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids
ATE441476T1 (de) 1996-08-30 2009-09-15 Upfront Chromatography As Isolierung von immunoglobulinen
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
US6214586B1 (en) 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
AU4314099A (en) * 1998-06-01 1999-12-20 Immune Response Corporation, The Novel method of large scale plasmid purification
EP0987327A1 (en) 1998-09-14 2000-03-22 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
KR100669305B1 (ko) 1999-07-23 2007-01-17 제넨테크, 인크. Ｒｎａ 분해효소 및 유기용매의 작용없이 접선 흐름여과를 이용한 플라스미드 ｄｎａ 정제법
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
DE10009982A1 (de) 2000-03-03 2001-09-06 Qiagen Gmbh Polymere Anionenaustauscher und deren Verwendung in chromatographischen Verfahren
EP1525299A2 (en) * 2002-07-26 2005-04-27 Applera Corporation Petal-array support for use with microplates

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105567672A (zh) * 2007-05-23 2016-05-11 Vgxi公司 包含高浓度的生物活性分子的组合物及其制备工艺
WO2016192012A1 (zh) * 2015-06-01 2016-12-08 易初丽 一种质粒纯化系统
CN106884011A (zh) * 2015-12-16 2017-06-23 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111088248A (zh) * 2019-12-23 2020-05-01 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法
CN111088248B (zh) * 2019-12-23 2020-10-16 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法

Also Published As

Publication number Publication date
US8093373B2 (en) 2012-01-10
EP1718744A1 (en) 2006-11-08
JP5095386B2 (ja) 2012-12-12
US20070161000A1 (en) 2007-07-12
WO2005083080A1 (en) 2005-09-09
SE0400490D0 (sv) 2004-02-26
JP2007525222A (ja) 2007-09-06
EP1718744B1 (en) 2013-11-13

Similar Documents

Publication Publication Date Title
CN1922319A (zh) 质粒纯化
Urthaler et al. Improved downstream process for the production of plasmid DNA for gene therapy.
JP7566762B2 (ja) 陰イオン交換クロマトグラフィーを使用した生物学的分子、例えばプラスミドｄｎａなどのための精製プロセス
JP2012050463A (ja) Ｄｎａ精製方法および精製されたｄｎａ
CN106102903A (zh) 用于生物颗粒纯化的分离基质
CN113416235B (zh) 用于纯化分离病毒类抗原的液相色谱法
KR20240034791A (ko) 아데노-연관 바이러스 캡시드를 분리하는 방법, 상기 방법에 의해 수득되는 조성물 및 그의 용도
WO2013130001A1 (en) Method for endotoxin removal
KR20240152327A (ko) 초나선 플라스미드 dna의 분리 방법
JP7586936B2 (ja) 混入ｄｎａをより効果的に除去するための、アデノ随伴ウイルスの精製の強化
JP5614936B2 (ja) アニオン交換基が固定された多孔膜を用いた核酸の精製方法
JP2003514655A (ja) 核酸構造を有する化合物を含むサンプル由来の物質の選択的除去方法
WO2024194157A1 (en) Chromatography medium and process
KR20250007522A (ko) 아데노-연관 바이러스 캡시드의 예비-스크리닝 방법 및 분리 방법
CN105646912B (zh) 多孔性纤维素粒子、色谱载体、病毒样颗粒的纯化方法和疫苗
JP2024527730A (ja) ウイルス捕捉の方法
JP2024510907A (ja) 関心対象のタンパク質を精製するための方法
CN118871580A (zh) 用于降低核酸纯化中的内毒素水平的方法
KR20260046476A (ko) 플라스미드 dna의 정제 방법
WO1998023630A1 (en) Matrix for rapid purification of nucleic acids

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
AD01 Patent right deemed abandoned

Effective date of abandoning: 20070228

C20 Patent right or utility model deemed to be abandoned or is abandoned