SE0400490D0 - Plasmid purification - Google Patents
Plasmid purificationInfo
- Publication number
- SE0400490D0 SE0400490D0 SE0400490A SE0400490A SE0400490D0 SE 0400490 D0 SE0400490 D0 SE 0400490D0 SE 0400490 A SE0400490 A SE 0400490A SE 0400490 A SE0400490 A SE 0400490A SE 0400490 D0 SE0400490 D0 SE 0400490D0
- Authority
- SE
- Sweden
- Prior art keywords
- plasmids
- matrix
- present
- pore
- eluent
- Prior art date
Links
- 239000013612 plasmid Substances 0.000 title abstract 7
- 238000000746 purification Methods 0.000 title 1
- 239000011159 matrix material Substances 0.000 abstract 6
- 239000011148 porous material Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 238000000926 separation method Methods 0.000 abstract 3
- 239000003480 eluent Substances 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 238000005349 anion exchange Methods 0.000 abstract 1
- 239000000969 carrier Substances 0.000 abstract 1
- 230000007717 exclusion Effects 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Saccharide Compounds (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0400490A SE0400490D0 (sv) | 2004-02-26 | 2004-02-26 | Plasmid purification |
| US10/589,740 US8093373B2 (en) | 2004-02-26 | 2005-02-21 | Plasmid purification |
| CNA2005800059786A CN1922319A (zh) | 2004-02-26 | 2005-02-21 | 质粒纯化 |
| PCT/SE2005/000229 WO2005083080A1 (en) | 2004-02-26 | 2005-02-21 | Plasmid purification |
| EP05711089.2A EP1718744B1 (en) | 2004-02-26 | 2005-02-21 | Plasmid purification |
| JP2007500711A JP5095386B2 (ja) | 2004-02-26 | 2005-02-21 | プラスミド精製 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0400490A SE0400490D0 (sv) | 2004-02-26 | 2004-02-26 | Plasmid purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SE0400490D0 true SE0400490D0 (sv) | 2004-02-26 |
Family
ID=32067270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE0400490A SE0400490D0 (sv) | 2004-02-26 | 2004-02-26 | Plasmid purification |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8093373B2 (https=) |
| EP (1) | EP1718744B1 (https=) |
| JP (1) | JP5095386B2 (https=) |
| CN (1) | CN1922319A (https=) |
| SE (1) | SE0400490D0 (https=) |
| WO (1) | WO2005083080A1 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2153260B1 (en) * | 2007-05-23 | 2017-01-25 | VGXI, Inc. | Composition comprising high concentration of biologically active molecules and processes for preparing the same |
| EP2315839A4 (en) * | 2008-08-25 | 2012-03-21 | Ge Healthcare Bio Sciences | EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA |
| EP2674753B1 (en) * | 2011-02-10 | 2020-05-13 | Sekisui Medical Co., Ltd. | Method for separating and detecting a nucleic acid strand |
| US9960620B2 (en) | 2014-09-16 | 2018-05-01 | Navitas Semiconductor, Inc. | Bootstrap capacitor charging circuit for GaN devices |
| KR101751700B1 (ko) | 2014-09-18 | 2017-06-30 | 한국과학기술연구원 | 전하를 띄는 다공성 구조체 |
| WO2016192012A1 (zh) * | 2015-06-01 | 2016-12-08 | 易初丽 | 一种质粒纯化系统 |
| CN106884011B (zh) * | 2015-12-16 | 2020-11-17 | 固安鼎泰海规生物科技有限公司 | 一种规模化质粒纯化的联合液相层析分离方法 |
| US10814305B2 (en) | 2016-09-29 | 2020-10-27 | Bio-Rad Laboratories, Inc. | Agarose-filled ceramic apatite |
| WO2018183465A1 (en) | 2017-03-28 | 2018-10-04 | Chiu Yin To | Method for accurate diagnosis of a disease targeting biomarkers in liquid biopsy |
| CN119425158A (zh) | 2017-06-01 | 2025-02-14 | 相达生物科技美国有限公司 | 用于多孔材料中双水相分离的相分离行为改性剂 |
| WO2019046563A1 (en) | 2017-09-01 | 2019-03-07 | Phase Diagnostics, Inc. | METHOD AND DEVICE FOR USING AQUEOUS BIPHASE SYSTEMS (ATPS) TO IMPROVE THE DIAGNOSIS OF SEXUALLY TRANSMITTED INFECTIONS |
| EP4303311A3 (en) | 2017-09-18 | 2024-04-03 | Phase Scientific International, Ltd. | Method for using aqueous two-phase system for the isolation, purification and/or concentration of short nucleic acid fragments |
| US11479765B2 (en) | 2018-01-19 | 2022-10-25 | Phase Scientific International, Ltd. | Method of isolating exosomes using encapsulation and aqueous micellar system |
| US12043826B2 (en) | 2018-01-19 | 2024-07-23 | Phase Scientific International, Ltd. | Spontaneous nucleic acid purification and concentration in a single step |
| CN111867721A (zh) * | 2018-01-19 | 2020-10-30 | 相达生物科技国际有限公司 | 使用固-液相系统分离和纯化核酸的方法 |
| CN111088248B (zh) * | 2019-12-23 | 2020-10-16 | 广州湾区生物科技有限公司 | 一种用于提取无内毒素质粒的磁性材料及其使用方法 |
| EP4106897A4 (en) | 2020-02-19 | 2023-10-25 | Bio-Rad Laboratories, Inc. | Method of preparing polymer-filled chromatography resin |
| EP4121530A1 (en) | 2020-03-20 | 2023-01-25 | Phase Scientific International, Ltd. | Compositions and methods for ribonucleic acid extraction |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE9601368D0 (sv) | 1996-04-11 | 1996-04-11 | Pharmacia Biotech Ab | Process for the production of a porous cross-linked polysaccharide gel |
| US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US6011148A (en) | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| ATE441476T1 (de) | 1996-08-30 | 2009-09-15 | Upfront Chromatography As | Isolierung von immunoglobulinen |
| SE9700769D0 (sv) | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
| US6214586B1 (en) | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| DE69922740T2 (de) | 1998-05-11 | 2005-12-08 | Tosoh Corp., Shinnanyo | Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie |
| AU4314099A (en) * | 1998-06-01 | 1999-12-20 | Immune Response Corporation, The | Novel method of large scale plasmid purification |
| EP0987327A1 (en) | 1998-09-14 | 2000-03-22 | QIAGEN GmbH | Novel method for purifying covalently closed circular DNA |
| US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| KR100669305B1 (ko) | 1999-07-23 | 2007-01-17 | 제넨테크, 인크. | Ｒｎａ 분해효소 및 유기용매의 작용없이 접선 흐름여과를 이용한 플라스미드 ｄｎａ 정제법 |
| SE9904272D0 (sv) * | 1999-11-25 | 1999-11-25 | Amersham Pharm Biotech Ab | A method for selective removal of a substance from samples containing compounds having nucleic acid structure |
| DE10009982A1 (de) | 2000-03-03 | 2001-09-06 | Qiagen Gmbh | Polymere Anionenaustauscher und deren Verwendung in chromatographischen Verfahren |
| EP1525299A2 (en) * | 2002-07-26 | 2005-04-27 | Applera Corporation | Petal-array support for use with microplates |
-
2004
- 2004-02-26 SE SE0400490A patent/SE0400490D0/xx unknown
-
2005
- 2005-02-21 WO PCT/SE2005/000229 patent/WO2005083080A1/en not_active Ceased
- 2005-02-21 CN CNA2005800059786A patent/CN1922319A/zh active Pending
- 2005-02-21 EP EP05711089.2A patent/EP1718744B1/en not_active Expired - Lifetime
- 2005-02-21 JP JP2007500711A patent/JP5095386B2/ja not_active Expired - Fee Related
- 2005-02-21 US US10/589,740 patent/US8093373B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US8093373B2 (en) | 2012-01-10 |
| EP1718744A1 (en) | 2006-11-08 |
| JP5095386B2 (ja) | 2012-12-12 |
| US20070161000A1 (en) | 2007-07-12 |
| WO2005083080A1 (en) | 2005-09-09 |
| CN1922319A (zh) | 2007-02-28 |
| JP2007525222A (ja) | 2007-09-06 |
| EP1718744B1 (en) | 2013-11-13 |
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