FR3038616B1 - Procede de purification et de concentration d'acides nucleiques. - Google Patents

Procede de purification et de concentration d'acides nucleiques. Download PDF

Info

Publication number
FR3038616B1
FR3038616B1 FR1556386A FR1556386A FR3038616B1 FR 3038616 B1 FR3038616 B1 FR 3038616B1 FR 1556386 A FR1556386 A FR 1556386A FR 1556386 A FR1556386 A FR 1556386A FR 3038616 B1 FR3038616 B1 FR 3038616B1
Authority
FR
France
Prior art keywords
purification
concentration
nucleic acids
complex
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1556386A
Other languages
English (en)
Other versions
FR3038616A1 (fr
Inventor
Celia Francois
Axelle Cadiere
Clement Faye
Laurent Garrelly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gl Biocontrol
Original Assignee
Gl Biocontrol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gl Biocontrol filed Critical Gl Biocontrol
Priority to FR1556386A priority Critical patent/FR3038616B1/fr
Priority to PCT/EP2016/065879 priority patent/WO2017005754A1/fr
Publication of FR3038616A1 publication Critical patent/FR3038616A1/fr
Application granted granted Critical
Publication of FR3038616B1 publication Critical patent/FR3038616B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Procédé pour purifier et concentrer au moins un fragment d'acides nucléiques d'une taille supérieure à 20 pb par formation d'un complexe entre un acide nucléique et un polymère polycationique en phase homogène, fixation du complexe sur une résine échangeuse de cations et élution par agitation mécanique.
FR1556386A 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques. Active FR3038616B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR1556386A FR3038616B1 (fr) 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques.
PCT/EP2016/065879 WO2017005754A1 (fr) 2015-07-06 2016-07-05 Procede de purification et de concentration d'acides nucleiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1556386A FR3038616B1 (fr) 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques.

Publications (2)

Publication Number Publication Date
FR3038616A1 FR3038616A1 (fr) 2017-01-13
FR3038616B1 true FR3038616B1 (fr) 2020-11-06

Family

ID=54478149

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1556386A Active FR3038616B1 (fr) 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques.

Country Status (2)

Country Link
FR (1) FR3038616B1 (fr)
WO (1) WO2017005754A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5506103A (en) * 1993-11-16 1996-04-09 Hybridon, Inc. Method for detecting charged oligonucleotides in biological fluids
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
DE102005005467A1 (de) 2005-02-04 2006-08-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbunde keramischer Hohlfasern, Verfahren zu deren Herstellung und deren Verwendung
AT501380B1 (de) * 2005-02-07 2007-11-15 Vitali Dipl Ing Dshemelinski Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat
FR2885130B1 (fr) 2005-04-28 2007-06-29 Centre Nat Rech Scient Procede de preparation de polylysines dendrimeres greffes
WO2008097342A2 (fr) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions et procédés d'isolation de molécules biologiques
JP5899731B2 (ja) * 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
CN113388606A (zh) * 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
WO2013181651A1 (fr) * 2012-06-01 2013-12-05 Omega Bio-Tek, Inc. Récupération de fragments d'acide nucléique sélective

Also Published As

Publication number Publication date
FR3038616A1 (fr) 2017-01-13
WO2017005754A1 (fr) 2017-01-12

Similar Documents

Publication Publication Date Title
PH12020500199A1 (en) Process for efficient purification of neutral human milk oligosaccharides (hmos) from microbial fermentation
CU20170039A7 (es) Método de purificación del anticuerpo humanizado 9e4
HRP20181047T1 (hr) Protutijela protiv kemokina pan-elr+ cxc
WO2010151632A8 (fr) Purification de protéines par précipitation de l'acide caprylique (l'acide octanoïque)
CY1118922T1 (el) Επεξεργασια ακατεργαστης αλμης απο εγκαταστασεις αφαλατωσης
EA201300524A1 (ru) Очистка антител ионообменной хроматографией в едином блоке
BR112015015931A2 (pt) método para separar e purificar ácidos carboxílicos de caldos de fermentação contendo sais de ácido carboxílico de amônio; e dispositivo para realização do método
MY181742A (en) Processes for producing acetic acid
MX2021004404A (es) Metodo para purificar anticuerpo que tiene bajo punto isoelectrico.
FR2985990A1 (fr) Nouveau procede de preparation de silices precipitees a morphologie, granulometrie et porosite particulieres
MX2022010103A (es) Procedimientos para producir acido acetico.
FR2994973B1 (fr) Procede de production d'acide methacrylique
FR3038616B1 (fr) Procede de purification et de concentration d'acides nucleiques.
RU2014127661A (ru) Композиция метионина, предназначенная для корма для животных
WO2011130809A3 (fr) Fractionnement d'ions à partir de solutions aqueuses
FR3004444B1 (fr) Procede de traitement d'eaux industrielles par separation physique, adsorption sur resine et osmose inverse, et installation correspondante
SG11201808954RA (en) Method for purifying methacrylic acid and method for producing methacrylic acid
FR2974802B1 (fr) Procede de purification de l'acide glycolique par distillation
FR2981060B1 (fr) Procede de valorisation des effluents d'acide chlorofluorhydrique
FR3057467B1 (fr) Procede de purification d'une solution aqueuse comprenant du diethylacetal
FR2985999A1 (fr) Procede de production d'acrylate de 2-octyle par transesterification
FR3034419B1 (fr) Procede de purification d'une proteine recombinante therapeutique a partir d'un lait transgenique
RU2010113825A (ru) Способ получения препарата на основе радия-224
CN106674422A (zh) 漆雾净化凝聚剂
RU2014118402A (ru) Способ очистки медьсодержащего сульфидно-щелочного смешанного стока

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20170113

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4

PLFP Fee payment

Year of fee payment: 5

PLFP Fee payment

Year of fee payment: 6

PLFP Fee payment

Year of fee payment: 7

PLFP Fee payment

Year of fee payment: 8

PLFP Fee payment

Year of fee payment: 9

PLFP Fee payment

Year of fee payment: 10