LT3663401T - Biologinių molekulių, tokių kaip plazmidinė dnr, gryninimo būdas, panaudojant anijonų mainų chromatografiją - Google Patents
Biologinių molekulių, tokių kaip plazmidinė dnr, gryninimo būdas, panaudojant anijonų mainų chromatografijąInfo
- Publication number
- LT3663401T LT3663401T LTEP19160132.7T LT19160132T LT3663401T LT 3663401 T LT3663401 T LT 3663401T LT 19160132 T LT19160132 T LT 19160132T LT 3663401 T LT3663401 T LT 3663401T
- Authority
- LT
- Lithuania
- Prior art keywords
- purification
- anion exchange
- plasmid dna
- exchange chromatography
- biological molecules
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19160132.7A EP3663401B1 (en) | 2019-02-28 | 2019-02-28 | Purification process for biological molecules such as plasmid dna using anionic exchange chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LT3663401T true LT3663401T (lt) | 2022-10-25 |
Family
ID=65801825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTEP19160132.7T LT3663401T (lt) | 2019-02-28 | 2019-02-28 | Biologinių molekulių, tokių kaip plazmidinė dnr, gryninimo būdas, panaudojant anijonų mainų chromatografiją |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20220145283A1 (lt) |
| EP (2) | EP3663401B1 (lt) |
| JP (1) | JP7566762B2 (lt) |
| KR (1) | KR102919038B1 (lt) |
| CN (1) | CN113518822A (lt) |
| CA (1) | CA3130855A1 (lt) |
| DK (1) | DK3663401T3 (lt) |
| ES (1) | ES2929470T3 (lt) |
| LT (1) | LT3663401T (lt) |
| PL (1) | PL3663401T3 (lt) |
| SG (1) | SG11202108893SA (lt) |
| WO (1) | WO2020174085A1 (lt) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3118156A1 (en) | 2018-12-12 | 2020-06-18 | Purilogics, LLC | Affinity membrane and method of preparation |
| CN113388609B (zh) * | 2021-06-21 | 2022-09-13 | 上海碧博生物医药科技有限公司 | 大型质粒dna的纯化方法 |
| CN119075384A (zh) * | 2021-07-12 | 2024-12-06 | 唐纳森公司 | 核苷酸和核苷酸组分的分离介质和使用其的纯化方法 |
| EP4234695A1 (en) * | 2022-02-24 | 2023-08-30 | Sartorius BIA Separations d.o.o. | Methods for the selective removal of contaminants during a process of purifying biologic agents |
| CN114907430B (zh) * | 2022-06-21 | 2023-12-01 | 中国科学院过程工程研究所 | 一种mRNA的分离纯化方法 |
| CN115337916B (zh) * | 2022-07-29 | 2023-10-24 | 福建卫生职业技术学院 | 一种水凝胶型细胞膜半制备色谱柱及其制备方法与应用 |
| CN115612684A (zh) * | 2022-11-01 | 2023-01-17 | 北京启辰生生物科技有限公司 | Gmp级质粒dna大规模简化生产方法 |
| EP4754251A1 (en) | 2023-08-02 | 2026-06-10 | Merck Patent GmbH | Method for purification of plasmid dna |
| CN116899633B (zh) * | 2023-09-13 | 2023-12-19 | 赛普(杭州)过滤科技有限公司 | 一种亲水阴离子交换层析介质及其制备方法与应用 |
| CN117434180A (zh) * | 2023-10-27 | 2024-01-23 | 苏州百因诺生物科技有限公司 | 可提高细胞裂解液中质粒dna分离度的液相色谱检测方法及质粒dna浓度的检测方法 |
| TW202615654A (zh) | 2024-08-12 | 2026-04-16 | 瑞士商赫孚孟拉羅股份公司 | 純化重組質體ｄｎａ的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6214586B1 (en) * | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
| US6270970B1 (en) * | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| EP1737945B1 (en) * | 2004-04-19 | 2011-01-26 | Aventis Pharma S.A. | Method for purifying plasmid dna |
| EP2088196A1 (en) * | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Methods and devices for producing biomolecules |
-
2019
- 2019-02-28 LT LTEP19160132.7T patent/LT3663401T/lt unknown
- 2019-02-28 ES ES19160132T patent/ES2929470T3/es active Active
- 2019-02-28 PL PL19160132.7T patent/PL3663401T3/pl unknown
- 2019-02-28 EP EP19160132.7A patent/EP3663401B1/en active Active
- 2019-02-28 DK DK19160132.7T patent/DK3663401T3/da active
-
2020
- 2020-02-28 SG SG11202108893SA patent/SG11202108893SA/en unknown
- 2020-02-28 EP EP20706520.2A patent/EP3931320A1/en active Pending
- 2020-02-28 US US17/434,442 patent/US20220145283A1/en active Pending
- 2020-02-28 WO PCT/EP2020/055285 patent/WO2020174085A1/en not_active Ceased
- 2020-02-28 JP JP2021550323A patent/JP7566762B2/ja active Active
- 2020-02-28 CN CN202080017232.1A patent/CN113518822A/zh active Pending
- 2020-02-28 CA CA3130855A patent/CA3130855A1/en active Pending
- 2020-02-28 KR KR1020217027475A patent/KR102919038B1/ko active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP7566762B2 (ja) | 2024-10-15 |
| EP3663401A1 (en) | 2020-06-10 |
| EP3931320A1 (en) | 2022-01-05 |
| DK3663401T3 (da) | 2022-11-28 |
| US20220145283A1 (en) | 2022-05-12 |
| SG11202108893SA (en) | 2021-09-29 |
| EP3663401B1 (en) | 2022-09-21 |
| WO2020174085A1 (en) | 2020-09-03 |
| KR20210134639A (ko) | 2021-11-10 |
| ES2929470T3 (es) | 2022-11-29 |
| PL3663401T3 (pl) | 2023-02-27 |
| KR102919038B1 (ko) | 2026-01-27 |
| CN113518822A (zh) | 2021-10-19 |
| JP2022523389A (ja) | 2022-04-22 |
| CA3130855A1 (en) | 2020-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PL3663401T3 (pl) | Sposób oczyszczania cząsteczek biologicznych takich jak plazmidowy DNA przy zastosowaniu chromatografii anionowymiennej | |
| IL285842A (en) | Improved process for dna integration using rna-guided endonucleases | |
| IL277472A (en) | Nucleic acid molecules for pseudouridylation | |
| DK3821011T5 (da) | Transposom-aktiveret dna/rna-sekventering (ted rna-seq) | |
| GB2598656B (en) | Methods for generating circular nucleic acid molecules | |
| IL282290A (en) | Methods for targeted insertion of dna in genes | |
| CO2017011957A2 (es) | Variantes de alfa-amilasa y polinucleótidos que codifican las mismas | |
| LT3423110T (lt) | Linijinis dnr dupleksas su uždarais galais, skirtas nevirusiniam geno pernešimui | |
| WO2017024317A3 (en) | Methods to induce targeted protein degradation through bifunctional molecules | |
| DK3348638T3 (da) | Fremgangsmåde til at konvertere genomsekvens fra gram-positiv bakterie ved specifikt at konvertere nukleinsyrebase i tilsigtet dna-sekvens, og molekylkompleks anvendt dertil | |
| IL311976A (en) | RNA molecules | |
| EP3450439B8 (en) | Method for producing monomer for single-stranded nucleic acid molecule | |
| WO2016100261A3 (en) | Method of treating cancer with cgamp or cgasmp | |
| WO2016161375A3 (en) | Methods of using oligonucleotide-guided argonaute proteins | |
| DK3565905T3 (da) | Nukleinsyresekvensering ved hjælp af affinitetsreagenser | |
| EP3436473A4 (en) | METHOD FOR PURIFYING FC FUSION PROTEINS | |
| TR201818614T4 (tr) | Bir Kromatografi Kolonunun Rejenerasyonuna Yönelik Yöntem | |
| IL276467A (en) | Production method for a nucleic acid molecule | |
| GB201711603D0 (en) | Method for constructing nucleic acid molecule | |
| EP3368692A4 (en) | MODIFYING 3 'END PIECES OF NUCLEIC ACIDS BY DNA POLYMERASE THETA | |
| EP3414255A4 (en) | PREPARATIVE ELECTROPHORETIC PROCESS FOR THE TARGETED CLEANING OF GENOMIC DNA FRAGMENTS | |
| EP3773609A4 (en) | TM-ENHANCING NUCLEOTIDES MODIFIED, DOUBLE STRANDED NUCLEIC ACID INHIBITOR MOLECULES | |
| EP3357507A4 (en) | NUCLEIC ACID DERIVATIVE WITH IMMUNOSTIMULATING EFFECT | |
| PT3494222T (pt) | Controlo de pragas de coleópteros usando moléculas de arn | |
| EP2975020A4 (en) | PROCESS FOR CLEANING 1,5-DIAMINOPENTANE |