SG11202108893SA - Purification process for biological molecules such as plasmid dna using anionic exchange chromatography - Google Patents

Purification process for biological molecules such as plasmid dna using anionic exchange chromatography

Info

Publication number
SG11202108893SA
SG11202108893SA SG11202108893SA SG11202108893SA SG11202108893SA SG 11202108893S A SG11202108893S A SG 11202108893SA SG 11202108893S A SG11202108893S A SG 11202108893SA SG 11202108893S A SG11202108893S A SG 11202108893SA SG 11202108893S A SG11202108893S A SG 11202108893SA
Authority
SG
Singapore
Prior art keywords
plasmid dna
exchange chromatography
purification process
biological molecules
anionic exchange
Prior art date
Application number
SG11202108893SA
Inventor
Andreas Zurbriggen
Ludovic Cosandey
Yves Balmer
Original Assignee
Lonza Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ag filed Critical Lonza Ag
Publication of SG11202108893SA publication Critical patent/SG11202108893SA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
SG11202108893SA 2019-02-28 2020-02-28 Purification process for biological molecules such as plasmid dna using anionic exchange chromatography SG11202108893SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19160132.7A EP3663401B1 (en) 2019-02-28 2019-02-28 Purification process for biological molecules such as plasmid dna using anionic exchange chromatography
PCT/EP2020/055285 WO2020174085A1 (en) 2019-02-28 2020-02-28 Purification process for biological molecules such as plasmid dna using anionic exchange chromatography

Publications (1)

Publication Number Publication Date
SG11202108893SA true SG11202108893SA (en) 2021-09-29

Family

ID=65801825

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202108893SA SG11202108893SA (en) 2019-02-28 2020-02-28 Purification process for biological molecules such as plasmid dna using anionic exchange chromatography

Country Status (12)

Country Link
US (1) US20220145283A1 (en)
EP (2) EP3663401B1 (en)
JP (1) JP2022523389A (en)
KR (1) KR20210134639A (en)
CN (1) CN113518822A (en)
CA (1) CA3130855A1 (en)
DK (1) DK3663401T3 (en)
ES (1) ES2929470T3 (en)
LT (1) LT3663401T (en)
PL (1) PL3663401T3 (en)
SG (1) SG11202108893SA (en)
WO (1) WO2020174085A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113388609B (en) * 2021-06-21 2022-09-13 上海碧博生物医药科技有限公司 Purification method of large plasmid DNA
EP4234695A1 (en) * 2022-02-24 2023-08-30 Sartorius BIA Separations d.o.o. Methods for the selective removal of contaminants during a process of purifying biologic agents
CN114907430B (en) * 2022-06-21 2023-12-01 中国科学院过程工程研究所 mRNA separation and purification method
CN115337916B (en) * 2022-07-29 2023-10-24 福建卫生职业技术学院 Hydrogel type cell membrane semi-preparation chromatographic column and preparation method and application thereof
CN115612684A (en) * 2022-11-01 2023-01-17 北京启辰生生物科技有限公司 GMP-grade plasmid DNA large-scale simplified production method
CN116899633B (en) * 2023-09-13 2023-12-19 赛普(杭州)过滤科技有限公司 Hydrophilic anion exchange chromatography medium and preparation method and application thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1737945B1 (en) * 2004-04-19 2011-01-26 Aventis Pharma S.A. Method for purifying plasmid dna
EP2088196A1 (en) * 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules

Also Published As

Publication number Publication date
JP2022523389A (en) 2022-04-22
CN113518822A (en) 2021-10-19
LT3663401T (en) 2022-10-25
PL3663401T3 (en) 2023-02-27
CA3130855A1 (en) 2020-09-03
WO2020174085A1 (en) 2020-09-03
DK3663401T3 (en) 2022-11-28
EP3663401B1 (en) 2022-09-21
EP3663401A1 (en) 2020-06-10
KR20210134639A (en) 2021-11-10
ES2929470T3 (en) 2022-11-29
EP3931320A1 (en) 2022-01-05
US20220145283A1 (en) 2022-05-12

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