JP2000501610A - 酵素類、それらの調製及びアクリル酸アンモニウムの製造へのそれらの使用 - Google Patents
酵素類、それらの調製及びアクリル酸アンモニウムの製造へのそれらの使用Info
- Publication number
- JP2000501610A JP2000501610A JP9521861A JP52186197A JP2000501610A JP 2000501610 A JP2000501610 A JP 2000501610A JP 9521861 A JP9521861 A JP 9521861A JP 52186197 A JP52186197 A JP 52186197A JP 2000501610 A JP2000501610 A JP 2000501610A
- Authority
- JP
- Japan
- Prior art keywords
- nitrilase
- acrylonitrile
- ammonium acrylate
- activity
- ammonium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/087—Acrylic polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. pH7.0においてアクリロニトリルに対し500μM以下のKmを有 することを特徴とするニトリラーゼ酵素。 2. pH7.0においてアクリロニトリルに対し100μM以下、好ましく は50μM以下のKmを有する請求項1記載のニトリラーゼ。 3. pH7.0においてアクリル酸アンモニウムに対し少なくとも100m MのKiを有することを特徴とするニトリラーゼ。 4. pH7.0においてアクリル酸アンモニウムに対し少なくとも250m MのKiを有する請求項3記載のニトリラーゼ。 5. 少なくとも200の(pH7.0におけるアクリル酸アンモニウムに対 するKi)/(pH7.0におけるアクリロニトリルに対するKm)比値を有す ることを特徴とするニトリラーゼ。 6. 少なくとも5000の前記比の値を有する請求項5記載のニトリラーゼ 。 7. pH6.8においてその最適pHにおけるアクリロニトリラーゼ活性の 少なくとも80%であるアクリロニトリラーゼ活性を有することを特徴とするニ トリラーゼ。 8. 50℃においてその25℃におけるアクリロニトリラーゼ活性の少なく とも80%を保持していることを特徴とするニトリラーゼ。 9. 以下の通り、冷却条件下に架橋ポリアクリルアミドビーズに固定化した 後にその元の比アクリロニトリラーゼ活性の少なくとも80%を保持しているこ とを特徴とするニトリラーゼ: ニトリラーゼを含有する細胞から成るペーストを冷却緩衝液中に懸濁させ、冷 却緩衝液中のアクリルアミド単量体及びメチレンビスアクリルアミド架橋剤の混 合物に添加し、次に、レドックス開始剤系の水溶性成分を即座に添加し、次に、 混合物を冷却鉱油及び界面活性剤を含有する攪拌樹脂ポットに移し、そして、両 液相に可溶性の第二のレドックス開始剤成分を添加して重合を開始して、細胞を 架橋重合体ビーズ中に閉じ込める。 10. 前記架橋ポリアクリルアミドビーズを60℃で12%の水分に乾燥及 び/又は24時間0.1mbarで凍結乾燥した後に、その固定化アクリロニト リラーゼ比活性の少なくとも90%を保持している請求項9記載のニトリラーゼ 。 11. 前記架橋ポリアクリルアミドビーズを20℃で17日間貯蔵した後に 、その固定化アクリロニトリラーゼ比活性の少なくとも90%を保持している請 求項9又は請求項10記載のニトリラーゼ。 12. 125〜175mMのアクリロニトリル及び2,475 〜2,525mMのアクリル酸アンモニウムを含有する水溶液中で測定して、少 なくとも5日の半減期を有していることを特徴とするニトリラーゼ。 13. ロドコッカス・ロドクラウスNCIMB 40757又はNCIMB 40833を培養することによって得ることができるニトリラーゼ。 14. ニトリラーゼを産生する能力を有するロドコッカス・ロドクラウスN CIMB 40757又はNCIMB 40833あるいはその変異体である微 生物。 15. 触媒としての請求項1〜13のいずれかに記載のニトリラーゼの存在 下、及び水の存在下に、アクリロニトリルをアクリル酸アンモニウムに転化する 方法。 16. 水の存在下にニトリルを検出する方法であって、 a) 請求項1〜16のいずれかに記載のニトリラーゼを、水の存在下にニ トリルと接触させ、 b) ニトリルをその対応するアンモニウム塩に転化させ、そして c) ニトリルの転化に関連する変化を検出する ことから成ることを特徴とする方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9525372.0A GB9525372D0 (en) | 1995-12-12 | 1995-12-12 | Enzymes, their preparation and their use in the production of ammonium acrylate |
GB9525372.0 | 1995-12-12 | ||
PCT/GB1996/003080 WO1997021805A1 (en) | 1995-12-12 | 1996-12-12 | Enzymes, their preparation and their use in the production of ammonium acrylate |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000501610A true JP2000501610A (ja) | 2000-02-15 |
JP3809192B2 JP3809192B2 (ja) | 2006-08-16 |
Family
ID=10785287
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52186197A Expired - Fee Related JP3809192B2 (ja) | 1995-12-12 | 1996-12-12 | 酵素類、それらの調製及びアクリル酸アンモニウムの製造へのそれらの使用 |
Country Status (15)
Country | Link |
---|---|
US (1) | US5998180A (ja) |
EP (1) | EP0870016B1 (ja) |
JP (1) | JP3809192B2 (ja) |
CN (1) | CN1207770A (ja) |
AT (1) | ATE235546T1 (ja) |
AU (1) | AU719269B2 (ja) |
BR (1) | BR9611961A (ja) |
CA (1) | CA2238443A1 (ja) |
DE (1) | DE69627021T2 (ja) |
DK (1) | DK0870016T3 (ja) |
ES (1) | ES2193280T3 (ja) |
GB (1) | GB9525372D0 (ja) |
PT (1) | PT870016E (ja) |
RU (1) | RU2188864C2 (ja) |
WO (1) | WO1997021805A1 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006055004A (ja) * | 2004-08-17 | 2006-03-02 | Asahi Kasei Chemicals Corp | 生体触媒を用いたカルボン酸(アンモニウム)の製造方法 |
JP2007512819A (ja) * | 2003-12-02 | 2007-05-24 | チバ スペシャルティ ケミカルズ ウォーター トリートメント リミテッド | 重合体を製造する方法 |
JP2008506397A (ja) * | 2004-07-19 | 2008-03-06 | チバ スペシャルティ ケミカルズ ウォーター トリートメント リミテッド | モノマー及びそのポリマーを調製する方法 |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6551804B2 (en) | 1999-07-12 | 2003-04-22 | E. I. Du Pont De Nemours And Company | Process for preparing 4-cyanopentanoic acid |
US7608445B1 (en) | 1999-12-29 | 2009-10-27 | Verenium Corporation | Nitrilases, nucleic acids encoding them and methods for making and using them |
US7521216B2 (en) | 1999-12-29 | 2009-04-21 | Verenium Corporation | Nitrilases and methods for making and using them |
US7300775B2 (en) | 1999-12-29 | 2007-11-27 | Verenium Corporation | Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents |
GB0002464D0 (en) * | 2000-02-04 | 2000-03-22 | Ciba Spec Chem Water Treat Ltd | Analysis of catalysed reactions by calorimetry |
DE10010149A1 (de) * | 2000-03-03 | 2001-09-06 | Basf Ag | Nitrilase aus Rhodococcus rhodochrous NCIMB 11216 |
US6455730B1 (en) | 2000-08-04 | 2002-09-24 | E. I. Du Pont De Nemours & Company | Preparation of dicarboxylic acid monoesters from cyanocarboxylic acid esters |
US6562603B2 (en) * | 2000-08-04 | 2003-05-13 | E. I. Du Pont De Nemours And Company | 3-hydroxycarboxylic acid production and use in branched polymers |
RU2177034C1 (ru) * | 2001-04-03 | 2001-12-20 | Закрытое акционерное общество "Биоамид" | Штамм бактерий alcaligenes denitrificans-продуцент нитрилазы |
US6670158B2 (en) | 2002-02-05 | 2003-12-30 | E. I. Du Pont De Nemours And Company | Method for producing methacrylic acid acrylic acid with a combination of enzyme catalysts |
AU2003251523A1 (en) | 2002-06-13 | 2003-12-31 | Diversa Corporation | Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid |
US20040109853A1 (en) * | 2002-09-09 | 2004-06-10 | Reactive Surfaces, Ltd. | Biological active coating components, coatings, and coated surfaces |
DE10312296B4 (de) * | 2003-03-20 | 2007-02-15 | Forschungszentrum Jülich GmbH | Reagenzloser Biosensor zum Nachweis von Nitrilen und Cyaniden |
US7198926B2 (en) * | 2003-05-08 | 2007-04-03 | E. I. Du Pont De Nemours And Company | Preparation of (E)- and (Z)-2-methyl-2-butenoic acids |
US8618066B1 (en) | 2003-07-03 | 2013-12-31 | Reactive Surfaces, Ltd., Llp | Coating compositions having peptidic antimicrobial additives and antimicrobial additives of other configurations |
US20050089987A1 (en) * | 2003-10-27 | 2005-04-28 | Lonza Ltd. | Polyacryamide beads containing encapsulated cells |
GB0327907D0 (en) * | 2003-12-02 | 2004-01-07 | Ciba Spec Chem Water Treat Ltd | Microorganism |
US7816106B2 (en) * | 2003-12-02 | 2010-10-19 | Ciba Specialty Chemicals Water Treatments Ltd. | Manufacture of amides |
US7393903B2 (en) * | 2004-08-04 | 2008-07-01 | Guerry Grune | Devices and methods for the rapid, reliable detection and determination of acrylamide concentration in food substances and prevention of acrylamide formation in the same |
WO2008106662A2 (en) | 2007-03-01 | 2008-09-04 | Verenium Corporation | Nitrilases, nucleic acids encoding them and methods for making and using them |
US7741088B2 (en) | 2007-10-31 | 2010-06-22 | E.I. Dupont De Nemours And Company | Immobilized microbial nitrilase for production of glycolic acid |
EP2338987A1 (en) * | 2009-11-16 | 2011-06-29 | ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KG | Whole cell biocatalyst |
US9102590B2 (en) | 2011-05-19 | 2015-08-11 | Mitsubishi Rayon Co., Ltd. | Method for producing acrylamide aqueous solution |
WO2012157775A1 (ja) | 2011-05-19 | 2012-11-22 | ダイヤニトリックス株式会社 | アクリルアミド水溶液の製造方法 |
WO2013059976A1 (en) | 2011-10-24 | 2013-05-02 | Rohm And Haas Company | Method for reducing odoriferous and/or toxic residual monomer in a latex |
EP3837376A1 (en) | 2018-08-16 | 2021-06-23 | AECI Limited | Rhodococcus rhodochrous strain and use thereof in the production of acrylic acid |
WO2020078798A1 (en) * | 2018-10-18 | 2020-04-23 | Basf Se | Method for the production of acrylic acid or salts thereof |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2245585B1 (ja) * | 1973-09-19 | 1976-05-14 | Anvar | |
IT1162484B (it) * | 1978-03-29 | 1987-04-01 | Nitto Chemical Industry Co Ltd | Procedimento pe produrre acrilammide o metacrilammide impiegando microorganismi |
JPS6019496A (ja) * | 1983-07-12 | 1985-01-31 | Nitto Chem Ind Co Ltd | 微生物によるアミド類の製造法 |
US4629700A (en) * | 1983-11-30 | 1986-12-16 | Standard Oil Company (Indiana) | Selective conversion of cyano compounds to amides and carboxylic acids |
JPS61162193A (ja) * | 1985-01-08 | 1986-07-22 | Nitto Chem Ind Co Ltd | 微生物によるアミド類の製造法 |
JPS61162191A (ja) * | 1985-01-11 | 1986-07-22 | Nitto Chem Ind Co Ltd | 微生物による有機酸類の製造法 |
JPS632596A (ja) * | 1986-06-20 | 1988-01-07 | Mitsubishi Heavy Ind Ltd | 超高圧型静水圧加圧装置 |
MX169933B (es) * | 1987-09-18 | 1993-08-02 | Hideaki Yamada | Procedimiento para la produccion biologica de amidas |
JP3009421B2 (ja) * | 1990-02-28 | 2000-02-14 | 秀明 山田 | 有機酸の生物学的製造法 |
JPH0771459B2 (ja) * | 1991-05-15 | 1995-08-02 | 三協食品工業株式会社 | 機能性食品素材とその製造方法 |
US5629190A (en) * | 1992-08-10 | 1997-05-13 | Rhone-Poulenc Chimie | Polypeptides possessing a nitrilase activity and method of converting nitriles to carboxylates by means of said polypeptides |
FR2708936B1 (fr) * | 1993-08-10 | 1995-11-10 | Rhone Poulenc Chimie | Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes. |
JP3437879B2 (ja) * | 1994-12-27 | 2003-08-18 | 三菱レイヨン株式会社 | 細菌の培養法 |
WO1997006248A1 (en) * | 1995-08-09 | 1997-02-20 | Allied Colloids Limited | Processes for the production of amidase |
WO1997021827A1 (en) * | 1995-12-12 | 1997-06-19 | Allied Colloids Limited | Production of ammonium acrylate |
-
1995
- 1995-12-12 GB GBGB9525372.0A patent/GB9525372D0/en active Pending
-
1996
- 1996-12-12 CA CA002238443A patent/CA2238443A1/en not_active Abandoned
- 1996-12-12 US US08/930,823 patent/US5998180A/en not_active Expired - Fee Related
- 1996-12-12 RU RU98113063/13A patent/RU2188864C2/ru not_active IP Right Cessation
- 1996-12-12 CN CN96199639A patent/CN1207770A/zh active Pending
- 1996-12-12 AT AT96941791T patent/ATE235546T1/de not_active IP Right Cessation
- 1996-12-12 WO PCT/GB1996/003080 patent/WO1997021805A1/en active IP Right Grant
- 1996-12-12 BR BR9611961A patent/BR9611961A/pt not_active IP Right Cessation
- 1996-12-12 ES ES96941791T patent/ES2193280T3/es not_active Expired - Lifetime
- 1996-12-12 PT PT96941791T patent/PT870016E/pt unknown
- 1996-12-12 AU AU11064/97A patent/AU719269B2/en not_active Ceased
- 1996-12-12 JP JP52186197A patent/JP3809192B2/ja not_active Expired - Fee Related
- 1996-12-12 EP EP96941791A patent/EP0870016B1/en not_active Expired - Lifetime
- 1996-12-12 DE DE69627021T patent/DE69627021T2/de not_active Expired - Fee Related
- 1996-12-12 DK DK96941791T patent/DK0870016T3/da active
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007512819A (ja) * | 2003-12-02 | 2007-05-24 | チバ スペシャルティ ケミカルズ ウォーター トリートメント リミテッド | 重合体を製造する方法 |
KR101175118B1 (ko) * | 2003-12-02 | 2012-08-21 | 시바 스페셜티 케미칼스 워터 트리트먼츠 리미티드 | 중합체의 제조방법 |
JP2008506397A (ja) * | 2004-07-19 | 2008-03-06 | チバ スペシャルティ ケミカルズ ウォーター トリートメント リミテッド | モノマー及びそのポリマーを調製する方法 |
JP2012139233A (ja) * | 2004-07-19 | 2012-07-26 | Ciba Specialty Chemicals Water Treatment Ltd | モノマー及びそのポリマーを調製する方法 |
KR101301025B1 (ko) * | 2004-07-19 | 2013-08-29 | 시바 스페셜티 케미칼스 워터 트리트먼츠 리미티드 | 단량체 및 이의 중합체의 제조방법 |
JP2006055004A (ja) * | 2004-08-17 | 2006-03-02 | Asahi Kasei Chemicals Corp | 生体触媒を用いたカルボン酸(アンモニウム)の製造方法 |
JP4553242B2 (ja) * | 2004-08-17 | 2010-09-29 | 旭化成ケミカルズ株式会社 | 生体触媒を用いたカルボン酸(アンモニウム)の製造方法 |
Also Published As
Publication number | Publication date |
---|---|
JP3809192B2 (ja) | 2006-08-16 |
CA2238443A1 (en) | 1997-06-19 |
EP0870016B1 (en) | 2003-03-26 |
US5998180A (en) | 1999-12-07 |
DE69627021D1 (de) | 2003-04-30 |
DE69627021T2 (de) | 2004-03-11 |
AU719269B2 (en) | 2000-05-04 |
ES2193280T3 (es) | 2003-11-01 |
RU2188864C2 (ru) | 2002-09-10 |
PT870016E (pt) | 2003-07-31 |
WO1997021805A1 (en) | 1997-06-19 |
CN1207770A (zh) | 1999-02-10 |
AU1106497A (en) | 1997-07-03 |
BR9611961A (pt) | 1999-02-17 |
ATE235546T1 (de) | 2003-04-15 |
EP0870016A1 (en) | 1998-10-14 |
GB9525372D0 (en) | 1996-02-14 |
DK0870016T3 (da) | 2003-06-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2000501610A (ja) | 酵素類、それらの調製及びアクリル酸アンモニウムの製造へのそれらの使用 | |
CA1298222C (en) | Process for biological production of amides | |
AU2007211036B2 (en) | Induction and stabilization of enzymatic activity in microorganisms | |
JP3163224B2 (ja) | 菌体または固定化菌体の懸濁液の保存方法 | |
EP1352965B1 (en) | Process for producing amide compound by using microbial catalyst | |
JPH0543351B2 (ja) | ||
JP2012196213A (ja) | 生体触媒の固定化 | |
JPH0448435B2 (ja) | ||
AU707650B2 (en) | Processes for the production of amidase | |
JPS63198990A (ja) | アクリルアミドの分解方法 | |
JP2004531243A (ja) | ニトリラーゼを使用してニトリルをカルボン酸に転換する改良された方法 | |
JP3664506B2 (ja) | D−乳酸及びl−ラクトアミドの製造法 | |
JP4709186B2 (ja) | 微生物触媒を用いたアミド化合物の製造方法 | |
AU2012202282B2 (en) | Induction and stabilization of enzymatic activity in microorganisms | |
KR830001446B1 (ko) | 고정화효소법에 의한 6-아미노 페니실란산의 제법 | |
AU2015203784A1 (en) | Induction and stabilization of enzymatic activity in microorganisms | |
JP2007295933A (ja) | 微生物触媒を用いたアミド化合物の製造方法 | |
JPH0773503B2 (ja) | アミドの生物学的製造法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20041109 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20041028 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20050203 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20050318 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20050509 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20060509 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20060522 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
LAPS | Cancellation because of no payment of annual fees |