IL92264A - A process for the expression of recombinant genes containing codons for arginine AGA and / or GGA in ILOC bacteria. E - Google Patents

A process for the expression of recombinant genes containing codons for arginine AGA and / or GGA in ILOC bacteria. E

Info

Publication number
IL92264A
IL92264A IL9226489A IL9226489A IL92264A IL 92264 A IL92264 A IL 92264A IL 9226489 A IL9226489 A IL 9226489A IL 9226489 A IL9226489 A IL 9226489A IL 92264 A IL92264 A IL 92264A
Authority
IL
Israel
Prior art keywords
gene
rna
expression
process according
coli
Prior art date
Application number
IL9226489A
Other languages
English (en)
Hebrew (he)
Other versions
IL92264A0 (en
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19893931933 external-priority patent/DE3931933A1/de
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of IL92264A0 publication Critical patent/IL92264A0/xx
Publication of IL92264A publication Critical patent/IL92264A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
IL9226489A 1988-11-11 1989-11-09 A process for the expression of recombinant genes containing codons for arginine AGA and / or GGA in ILOC bacteria. E IL92264A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE3838378 1988-11-11
DE3928899 1989-08-31
DE19893931933 DE3931933A1 (de) 1988-11-11 1989-09-25 Verfahren zur expression eines eukaryontischen gens

Publications (2)

Publication Number Publication Date
IL92264A0 IL92264A0 (en) 1990-07-26
IL92264A true IL92264A (en) 1994-08-31

Family

ID=27198503

Family Applications (1)

Application Number Title Priority Date Filing Date
IL9226489A IL92264A (en) 1988-11-11 1989-11-09 A process for the expression of recombinant genes containing codons for arginine AGA and / or GGA in ILOC bacteria. E

Country Status (10)

Country Link
US (1) US6270988B1 (es)
EP (1) EP0373365B1 (es)
JP (1) JP2619077B2 (es)
AT (1) ATE105863T1 (es)
AU (1) AU618640B2 (es)
CA (1) CA2002516C (es)
DE (1) DE58907690D1 (es)
DK (1) DK175456B1 (es)
ES (1) ES2053917T3 (es)
IL (1) IL92264A (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3838377A1 (de) * 1988-11-11 1990-05-23 Boehringer Mannheim Gmbh Expressions-enhancer und dessen verwendung zur erhoehung der ausbeute bei der expression von rekombinanten genen
DE60042396D1 (de) * 1999-01-27 2009-07-30 Carstens Carsten Peter Hohe expression eines heterologen proteins mit seltenen codons
US20110097709A1 (en) * 2000-03-13 2011-04-28 Kidd Geoffrey L Method for modifying a nucleic acid
US6821755B2 (en) 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
DE10037111A1 (de) * 2000-07-27 2002-02-07 Boehringer Ingelheim Int Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle
AU2002305052A1 (en) 2001-03-13 2002-09-24 Corvas International, Inc. Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
KR20040011480A (ko) 2001-03-22 2004-02-05 덴드레온 샌 디에고 엘엘씨 세린 프로테아제 ｃｖｓｐ14를 암호화하는 핵산 분자,암호화된 폴리펩티드 및 이에 근거한 방법
WO2002077267A2 (en) 2001-03-27 2002-10-03 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembran serine protease 9, the encoded polypeptides and methods based thereon
CA2447050A1 (en) 2001-05-14 2002-11-21 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
US20030134298A1 (en) * 2001-07-03 2003-07-17 Madison Edwin L. Nucleic acid molecules encoding a transmembrane serine protease 20, the encoded polypeptides and methods based thereon
MXPA04000397A (es) * 2001-07-19 2004-03-18 Yissum Res Dev Co Polipeptidos que tienen actividad catalitica de isomerasa de carotenoides, acidos nucleicos que los codifican y usos de los mismos.
US20030134794A1 (en) * 2001-11-20 2003-07-17 Madison Edwin L. Nucleic acid molecules encoding serine protease 17, the encoded polypeptides and methods based thereon
JP2007525981A (ja) 2004-03-03 2007-09-13 エフ．ホフマン−ラ　ロシュ　アーゲー 肝細胞成長因子のｎ末端フラグメントを組み換え発現するための方法
JP5232138B2 (ja) * 2006-04-22 2013-07-10 スカラブ ゲノミクス， エルエルシー ｔＲＮＡ遺伝子をしようする、組み換えタンパク質産生のための方法および組成物
JP4886654B2 (ja) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 ヒト成長ホルモンの効率化された製造方法
KR101147860B1 (ko) 2008-09-22 2012-05-25 한국과학기술원 특정 아미노산의 ｔＲＮＡ와의 동시 발현을 통한 특정 아미노산 함량이 높은 단백질의 제조방법
KR101317420B1 (ko) * 2010-03-11 2013-10-10 한국과학기술원 고분자량의 재조합 실크 또는 실크 유사 단백질 및 이를 이용하여 제조된 마이크로 또는 나노 크기의 거미줄 또는 거미줄 유사 섬유

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4654307A (en) * 1983-02-17 1987-03-31 The Research Foundation Of State University Of New York Novel bacteria containing a plasmid having a tRNA code
SE455704B (sv) * 1983-10-24 1988-08-01 Ac Biotechnics Ab Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen
US5145776A (en) * 1987-01-14 1992-09-08 President & Fellows Of Harvard College Method of using T7 DNA polymerase to mutagenize and fill-in DNA
US4970147A (en) * 1988-03-17 1990-11-13 The General Hospital Corporation Oxygen regulatable gene expression

Also Published As

Publication number Publication date
DK564789A (da) 1990-05-12
EP0373365B1 (de) 1994-05-18
DK564789D0 (da) 1989-11-10
AU618640B2 (en) 1992-01-02
IL92264A0 (en) 1990-07-26
EP0373365A1 (de) 1990-06-20
US6270988B1 (en) 2001-08-07
DK175456B1 (da) 2004-11-01
CA2002516A1 (en) 1990-05-11
CA2002516C (en) 1995-10-31
JPH02265490A (ja) 1990-10-30
ES2053917T3 (es) 1994-08-01
ATE105863T1 (de) 1994-06-15
AU4444389A (en) 1990-11-08
JP2619077B2 (ja) 1997-06-11
DE58907690D1 (de) 1994-06-23

Similar Documents

Publication Publication Date Title
CA2002516C (en) Process for the expression of a recombinant gene
JP3801389B2 (ja) 栄養要求性を相補することによる、抗生物質によらない選抜に基づく新規の大腸菌／宿主ベクター系
Carter-Muenchau et al. Growth-rate-dependent regulation of 6-phosphogluconate dehydrogenase level mediated by an anti-Shine-Dalgarno sequence located within the Escherichia coli gnd structural gene.
FI86746B (fi) Foerfarande foer framstaellning av maensklig vaevnads-plasminogenaktivator (tpa) med hjaelp av saccharomyces cerevisiae -jaest och i foerfarandet anvaenda hybridvektorer.
AU608572B2 (en) Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase and deacetylcephalosporin c synthetase
JP2544602B2 (ja) 新規プラスミドベクタ−
CA2622710C (en) Hybrid portable origin of replication plasmids
KR0167761B1 (ko) 플라스미드, 이의 작제방법 및 플라스미노겐 활성자 제조에 있어서의 이의 용도
EP0211260B1 (en) Chromosomal dna sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
AU640654B2 (en) Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
EP0186069B1 (en) New expression control sequence
Hottinger et al. Allele-specific complementation of an Escherichia coli leuB mutation by a Lactobacillus bulgaricus tRNA gene
CA2012632C (en) Process for the expression of a recombinant gene
CA2002515C (en) Expression enhancer and use thereof to increase the yield in the expression of recombinant genes
US5160489A (en) Expression vectors and method for their construction
EP0154351B1 (en) Expression plasmids useful in b. subtilis
AU601420B2 (en) Process for the production of plasminogen activators in procaryotes
JP3877861B2 (ja) 高発現ベクタープラスミド用ｄｎａ断片
Nicholls et al. Repressor gene, blaI, for Bacillus licheniformis 749 β‐lactamase
DE3931933A1 (de) Verfahren zur expression eines eukaryontischen gens
JPS63133988A (ja) 変形された組織プラスミノ−ゲンアクチベ−タ−
MXPA99006530A (es) Nuevo sistema hospedador/vector de escherichia coli basado en la seleccion libre de antibioticos mediante complementacion de auxotrofia

Legal Events

Date Code Title Description
KB Patent renewed
KB Patent renewed
KB Patent renewed
KB Patent renewed
KB Patent renewed