DK564789A - Fremgangsmaade til ekspression af et eukaryot gen - Google Patents

Fremgangsmaade til ekspression af et eukaryot gen

Info

Publication number
DK564789A
DK564789A DK564789A DK564789A DK564789A DK 564789 A DK564789 A DK 564789A DK 564789 A DK564789 A DK 564789A DK 564789 A DK564789 A DK 564789A DK 564789 A DK564789 A DK 564789A
Authority
DK
Denmark
Prior art keywords
coli
aga
amount
agg
codons
Prior art date
Application number
DK564789A
Other languages
English (en)
Other versions
DK564789D0 (da
DK175456B1 (da
Inventor
Ulrich Brinkmann
Ralf Mattes
Stephan Fischer
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19893931933 external-priority patent/DE3931933A1/de
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of DK564789D0 publication Critical patent/DK564789D0/da
Publication of DK564789A publication Critical patent/DK564789A/da
Application granted granted Critical
Publication of DK175456B1 publication Critical patent/DK175456B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)
  • Peptides Or Proteins (AREA)
DK564789A 1988-11-11 1989-11-10 Fremgangsmåde til ekspression af et eukaryot gen DK175456B1 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE3838378 1988-11-11
DE3838378 1988-11-11
DE3928899 1989-08-31
DE3928899 1989-08-31
DE19893931933 DE3931933A1 (de) 1988-11-11 1989-09-25 Verfahren zur expression eines eukaryontischen gens
DE3931933 1989-09-25

Publications (3)

Publication Number Publication Date
DK564789D0 DK564789D0 (da) 1989-11-10
DK564789A true DK564789A (da) 1990-05-12
DK175456B1 DK175456B1 (da) 2004-11-01

Family

ID=27198503

Family Applications (1)

Application Number Title Priority Date Filing Date
DK564789A DK175456B1 (da) 1988-11-11 1989-11-10 Fremgangsmåde til ekspression af et eukaryot gen

Country Status (10)

Country Link
US (1) US6270988B1 (da)
EP (1) EP0373365B1 (da)
JP (1) JP2619077B2 (da)
AT (1) ATE105863T1 (da)
AU (1) AU618640B2 (da)
CA (1) CA2002516C (da)
DE (1) DE58907690D1 (da)
DK (1) DK175456B1 (da)
ES (1) ES2053917T3 (da)
IL (1) IL92264A (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3838377A1 (de) * 1988-11-11 1990-05-23 Boehringer Mannheim Gmbh Expressions-enhancer und dessen verwendung zur erhoehung der ausbeute bei der expression von rekombinanten genen
ATE434051T1 (de) * 1999-01-27 2009-07-15 Stratagene California Hohe expression eines heterologen proteins mit seltenen codons
US20110097709A1 (en) * 2000-03-13 2011-04-28 Kidd Geoffrey L Method for modifying a nucleic acid
US6821755B2 (en) 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
DE10037111A1 (de) * 2000-07-27 2002-02-07 Boehringer Ingelheim Int Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle
WO2002072786A2 (en) 2001-03-13 2002-09-19 Corvas International, Inc. Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
US7172892B2 (en) 2001-03-22 2007-02-06 Dendreon Corporation Nucleic acid molecules encoding serine protease CVSP14, the encoded polypeptides and methods based thereon
EP1379637A4 (en) 2001-03-27 2005-04-06 Dendreon Corp TRANSMEMBRANE SERINE PROTEASE 9 CODING NUCLEIC ACID MOLECULES, THE POLYPEPTIDE CODED, AND METHODS FORMING THEREOF
JP2005506832A (ja) 2001-05-14 2005-03-10 デンドレオン・サンディエゴ・リミテッド・ライアビリティ・カンパニー トランスメンブランセリンプロテアーゼ１０をコードする核酸分子、コードされるポリペプチドおよびそれに基づく方法
WO2003004681A2 (en) * 2001-07-03 2003-01-16 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembrane serine protease 20, the encoded polypeptides and methods based thereon
IL159944A0 (en) * 2001-07-19 2004-06-20 Yissum Res Dev Co Polypeptides having carotenoid isomerase catalytic activity, nucleic acids encoding same and uses thereof
WO2003044179A2 (en) * 2001-11-20 2003-05-30 Dendreon San Diego Llc Nucleic acid molecules encoding serine protease 17, the encoded polypeptides and methods based thereon
CA2558742A1 (en) 2004-03-03 2005-10-13 F. Hoffmann-La Roche Ag Method for the recombinant expression of an n-terminal fragment of hepatocyte growth factor
JP5232138B2 (ja) * 2006-04-22 2013-07-10 スカラブ ゲノミクス， エルエルシー ｔＲＮＡ遺伝子をしようする、組み換えタンパク質産生のための方法および組成物
JP4886654B2 (ja) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 ヒト成長ホルモンの効率化された製造方法
KR101147860B1 (ko) 2008-09-22 2012-05-25 한국과학기술원 특정 아미노산의 ｔＲＮＡ와의 동시 발현을 통한 특정 아미노산 함량이 높은 단백질의 제조방법
KR101317420B1 (ko) * 2010-03-11 2013-10-10 한국과학기술원 고분자량의 재조합 실크 또는 실크 유사 단백질 및 이를 이용하여 제조된 마이크로 또는 나노 크기의 거미줄 또는 거미줄 유사 섬유

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4654307A (en) * 1983-02-17 1987-03-31 The Research Foundation Of State University Of New York Novel bacteria containing a plasmid having a tRNA code
SE455704B (sv) * 1983-10-24 1988-08-01 Ac Biotechnics Ab Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen
US5145776A (en) * 1987-01-14 1992-09-08 President & Fellows Of Harvard College Method of using T7 DNA polymerase to mutagenize and fill-in DNA
US4970147A (en) * 1988-03-17 1990-11-13 The General Hospital Corporation Oxygen regulatable gene expression

Also Published As

Publication number Publication date
ATE105863T1 (de) 1994-06-15
DE58907690D1 (de) 1994-06-23
IL92264A0 (en) 1990-07-26
ES2053917T3 (es) 1994-08-01
IL92264A (en) 1994-08-31
JP2619077B2 (ja) 1997-06-11
US6270988B1 (en) 2001-08-07
EP0373365B1 (de) 1994-05-18
DK564789D0 (da) 1989-11-10
AU618640B2 (en) 1992-01-02
JPH02265490A (ja) 1990-10-30
EP0373365A1 (de) 1990-06-20
DK175456B1 (da) 2004-11-01
AU4444389A (en) 1990-11-08
CA2002516C (en) 1995-10-31
CA2002516A1 (en) 1990-05-11

Similar Documents

Publication Publication Date Title
DK564789D0 (da) Fremgangsmaade til ekspression af et eukaryot gen
FI963246A (fi) Geeniterapiassa käytettävä isäntävektorijärjestelmä
WO1991011525A3 (en) Vaccines
EP0722461A4 (en) EXPRESSION OF FUSION POLYPEPTIDES TRANSPORTED OUT OF CYTOPLASM WITHOUT HEAD SEQUENCE
DK364585A (da) Genteknologisk fremgangsmaade til fremstilling af hinrudiner og middeltil udfoerelse af fremgangsmaaden
PT83065A (de) Fusionsproteine verfahren zu ihrer herstellung und ihre verwendung
NZ225351A (en) Genetically engineered inducible virus resistance in plants
GR860914B (en) Expression of immunologically reactive viral proteins
DK0388963T3 (da) Fremgangsmåde til ekspression af et rekombinant gen
DK0396436T3 (da) Gærstammer til fremstilling af modne heterologe proteiner, især hirudin
EP0353812A3 (en) A process for the rescue of dna and for detecting mutations in marker genes
AU5958694A (en) Synthetic herpes simplex virus promoters
DK391185D0 (da) Rekombinant saccharomyces
IL92265A0 (en) Expression enhancer and employment thereof to increase the yield in the expression of recombinant genes

Legal Events

Date Code Title Description
PUP Patent expired