EP0353812A3 - A process for the rescue of dna and for detecting mutations in marker genes - Google Patents

A process for the rescue of dna and for detecting mutations in marker genes Download PDF

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Publication number
EP0353812A3
EP0353812A3 EP89201895A EP89201895A EP0353812A3 EP 0353812 A3 EP0353812 A3 EP 0353812A3 EP 89201895 A EP89201895 A EP 89201895A EP 89201895 A EP89201895 A EP 89201895A EP 0353812 A3 EP0353812 A3 EP 0353812A3
Authority
EP
European Patent Office
Prior art keywords
dna
rescue
marker genes
detecting mutations
cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP89201895A
Other versions
EP0353812B1 (en
EP0353812A2 (en
Inventor
Jan Vijg
Jan Albert Gossen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Original Assignee
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19852648&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP0353812(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO filed Critical Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority to AT89201895T priority Critical patent/ATE100151T1/en
Priority to EP93200367A priority patent/EP0548056B2/en
Publication of EP0353812A2 publication Critical patent/EP0353812A2/en
Publication of EP0353812A3 publication Critical patent/EP0353812A3/en
Application granted granted Critical
Publication of EP0353812B1 publication Critical patent/EP0353812B1/en
Priority to GR960400333T priority patent/GR3018927T3/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a process for the cloning of a DNA sequence wherein a plasmid vector containing said DNA sequence is multiplied in bacterial host which is incapable of host restriction, such as the Escherichia coli C strain. The invention may be used for cloning DNA sequences originating from a mammalian genome. <IMAGE>
EP89201895A 1988-07-19 1989-07-18 A process for the rescue of DNA and for detecting mutations in marker genes Expired - Lifetime EP0353812B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT89201895T ATE100151T1 (en) 1988-07-19 1989-07-18 METHODS FOR DNA RECOVERY AND DETECTION OF MUTATIONS IN MARKER GENES.
EP93200367A EP0548056B2 (en) 1988-07-19 1989-07-18 A process for cloning methylated DNA in a bacterial host incapable of host restriction
GR960400333T GR3018927T3 (en) 1988-07-19 1996-02-07 A process for cloning methylated DNA in a bacterial host incapable of host restriction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8801826A NL8801826A (en) 1988-07-19 1988-07-19 METHOD FOR DETECTING MUTATIONS IN MARKER GENES
NL8801826 1988-07-19

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP93200367.6 Division-Into 1989-07-18
EP93200367A Division EP0548056B2 (en) 1988-07-19 1989-07-18 A process for cloning methylated DNA in a bacterial host incapable of host restriction

Publications (3)

Publication Number Publication Date
EP0353812A2 EP0353812A2 (en) 1990-02-07
EP0353812A3 true EP0353812A3 (en) 1990-03-07
EP0353812B1 EP0353812B1 (en) 1994-01-12

Family

ID=19852648

Family Applications (3)

Application Number Title Priority Date Filing Date
EP89201895A Expired - Lifetime EP0353812B1 (en) 1988-07-19 1989-07-18 A process for the rescue of DNA and for detecting mutations in marker genes
EP95201753A Withdrawn EP0683238A3 (en) 1988-07-19 1989-07-18 A process for the cloning of DNA
EP93200367A Expired - Lifetime EP0548056B2 (en) 1988-07-19 1989-07-18 A process for cloning methylated DNA in a bacterial host incapable of host restriction

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP95201753A Withdrawn EP0683238A3 (en) 1988-07-19 1989-07-18 A process for the cloning of DNA
EP93200367A Expired - Lifetime EP0548056B2 (en) 1988-07-19 1989-07-18 A process for cloning methylated DNA in a bacterial host incapable of host restriction

Country Status (8)

Country Link
US (1) US5470706A (en)
EP (3) EP0353812B1 (en)
JP (1) JPH0829093B2 (en)
AT (2) ATE132529T1 (en)
DE (2) DE68925369T3 (en)
ES (2) ES2061943T3 (en)
GR (1) GR3018927T3 (en)
NL (1) NL8801826A (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL9100567A (en) * 1991-04-02 1992-11-02 Ingeny Bv METHOD FOR DETECTING MUTATIONS, TRANSGEN MAMMAL, TRANSGENIC MAMMAL CELL, AND METHOD FOR EXAMINING SUBSTANCES OR CONDITIONS FOR MUTAGENIC PROPERTIES
CA2130081A1 (en) * 1992-02-14 1993-08-19 Jay M. Short Mutagenesis testing using transgenic non-human animals carrying test dna sequences
AU4220093A (en) * 1992-04-27 1993-11-29 Indiana University Foundation Live animal mutagenesis systems for testing mutagenic agents in vivo
CA2154265C (en) * 1993-01-21 2011-01-04 Spencer B. Farr Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound
DE69926629T2 (en) 1998-05-31 2006-06-08 University Of Georgia Research Foundation, Inc. TRANSGENIC FISH CARRYING A TRANSGENIC CONSTRUCT FOR THE DETECTION OF MUTATIONS DERIVED FROM BACTERIOPHAGES
AU1323000A (en) 1998-10-26 2000-05-15 University Of Georgia Research Foundation, Inc., The Transgenic fish carrying plasmid for mutation detection and methods
GB9924973D0 (en) * 1999-10-21 1999-12-22 Cobra Therapeutics Ltd Preparation of cellular components that are substantially free of chromosomal DNA
US7255999B2 (en) * 2001-05-21 2007-08-14 Monogram Biosciences, Inc. Methods and compositions for analyzing proteins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0289121A2 (en) * 1987-05-01 1988-11-02 Stratagene Mutagenesis testing using transgenic non-human animals carrying test DNA sequences

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4753874A (en) * 1985-07-08 1988-06-28 Board Of Trustees Of The Leland Stanford Junior University Rapid mutation testing system for human cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0289121A2 (en) * 1987-05-01 1988-11-02 Stratagene Mutagenesis testing using transgenic non-human animals carrying test DNA sequences

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 107, 1987, abstract no. 110455n, Columbus, Ohio, US; V.M. KRAMAROV et al.: "Recombinant plasmid pBP5RM and method for constructing it and Escherichia coli strain C 600 P5 RM - a producer of site-specific endonuclease V and II", & OTKRYTIYA, IZOBRET 1987, (9), 110 *
MECHANISMS OF AGEING AND DEVELOPMENT, vol. 41, 1987, pages 47-63, Elsevier scientific publishers ireland ltd., IR; J. VIJG et al.: "A search for DNA alterations in the aging mammalian genome: an experimental strategy" *
METHODS IN ENZYMOLOGY, vol. 68, 1979, pages 309-626, Academic Press, US; J. COLLINS: "Escherichia coli plasmids packageable in vitro in a bacteriophage particles" *
MUTATION RESEARCH, vol. 181, 1987, pages 227-234, Elsevier science publishers B.V. (Biomedical Division), Amsterdam, NL; P.H.M. LOHMAN et al.: "DNA methods for detecting and analyzing mutations in vivo" *
NUCLEIC ACIDS RESEARCH, vol. 16, no. 19, 11th October 1988, page 9343, Oxford, GB; J. GOSSEN et al.: "E. Coli C: a convenient host strain for rescue of highly methylated DNA" *
NUCLEIC ACIDS RESEARCH, vol. 16, no. 4, 25th February 1988, pages 1563-1575, IRL Press Ltd, Oxford, GB; E.A. RALEIGH et al.: "McrA and McrB restriction phenotypes of some E.coli strains and implications for gene cloning" *

Also Published As

Publication number Publication date
ATE132529T1 (en) 1996-01-15
EP0353812B1 (en) 1994-01-12
ES2081679T3 (en) 1996-03-16
EP0683238A2 (en) 1995-11-22
DE68925369T2 (en) 1996-05-15
EP0683238A3 (en) 1995-12-20
DE68925369D1 (en) 1996-02-15
DE68912228T2 (en) 1994-08-04
DE68925369T3 (en) 2001-08-09
GR3018927T3 (en) 1996-05-31
EP0548056B2 (en) 2001-04-04
DE68912228D1 (en) 1994-02-24
JPH0829093B2 (en) 1996-03-27
ATE100151T1 (en) 1994-01-15
EP0548056B1 (en) 1996-01-03
EP0548056A1 (en) 1993-06-23
US5470706A (en) 1995-11-28
EP0353812A2 (en) 1990-02-07
ES2061943T3 (en) 1994-12-16
NL8801826A (en) 1990-02-16
JPH02142471A (en) 1990-05-31

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