ES2053917T3 - Procedimiento para la expresion de un gen recombinante. - Google Patents

Procedimiento para la expresion de un gen recombinante.

Info

Publication number
ES2053917T3
ES2053917T3 ES89120895T ES89120895T ES2053917T3 ES 2053917 T3 ES2053917 T3 ES 2053917T3 ES 89120895 T ES89120895 T ES 89120895T ES 89120895 T ES89120895 T ES 89120895T ES 2053917 T3 ES2053917 T3 ES 2053917T3
Authority
ES
Spain
Prior art keywords
coli
recombinant gene
expression
aga
amount
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89120895T
Other languages
English (en)
Inventor
Ulrich Dipl-Biol Brinkmann
Ralf Mattes
Stephan Dipl-Biol Fischer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19893931933 external-priority patent/DE3931933A1/de
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2053917T3 publication Critical patent/ES2053917T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Abstract

PARA EXPRESAR UNOS GENES RECOMBINANTES QUE CONTIENEN CODONS AGA Y/O AGG PARA ARGININA EN E COLI SEGUN LA TRANSFORMACION CON UN VECTOR EXPRESION QUE CONTIENE EL GEN RECOMBINANTE, SE ELEVA LA CANTIDAD DE T-RNA CINCO VECES LA CANTIDAD NORMAL PRELLEVADA EN ESTA CELULA O SELECCIONA UN TRONCO QUE CONTIENE UNA CANTIDAD EN T-RNA CINCO VECES LA CANTIDAD NORMAL PRELLEVADA EN E COLI Y SE EMPLEA ESTE TRONCO COMO CELULA ACTIVA. EL T-RNA INCORPORA LA ARGININA Y RECONOCE LOS CODONS AGG Y AGA.
ES89120895T 1988-11-11 1989-11-10 Procedimiento para la expresion de un gen recombinante. Expired - Lifetime ES2053917T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE3838378 1988-11-11
DE3928899 1989-08-31
DE19893931933 DE3931933A1 (de) 1988-11-11 1989-09-25 Verfahren zur expression eines eukaryontischen gens

Publications (1)

Publication Number Publication Date
ES2053917T3 true ES2053917T3 (es) 1994-08-01

Family

ID=27198503

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89120895T Expired - Lifetime ES2053917T3 (es) 1988-11-11 1989-11-10 Procedimiento para la expresion de un gen recombinante.

Country Status (10)

Country Link
US (1) US6270988B1 (es)
EP (1) EP0373365B1 (es)
JP (1) JP2619077B2 (es)
AT (1) ATE105863T1 (es)
AU (1) AU618640B2 (es)
CA (1) CA2002516C (es)
DE (1) DE58907690D1 (es)
DK (1) DK175456B1 (es)
ES (1) ES2053917T3 (es)
IL (1) IL92264A (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3838377A1 (de) * 1988-11-11 1990-05-23 Boehringer Mannheim Gmbh Expressions-enhancer und dessen verwendung zur erhoehung der ausbeute bei der expression von rekombinanten genen
DE60042396D1 (de) * 1999-01-27 2009-07-30 Carstens Carsten Peter Hohe expression eines heterologen proteins mit seltenen codons
WO2001068835A2 (en) * 2000-03-13 2001-09-20 Aptagen Method for modifying a nucleic acid
US6821755B2 (en) 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
DE10037111A1 (de) * 2000-07-27 2002-02-07 Boehringer Ingelheim Int Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle
US7125703B2 (en) 2001-03-13 2006-10-24 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 7, the encoded polypeptides and methods based thereon
JP2004535166A (ja) 2001-03-22 2004-11-25 デンドレオン・サンディエゴ・リミテッド・ライアビリティ・カンパニー セリンプロテアーゼｃｖｓｐ１４をコードする核酸分子、コードされるポリペプチドおよびそれに基づく方法
EP1379637A4 (en) 2001-03-27 2005-04-06 Dendreon Corp TRANSMEMBRANE SERINE PROTEASE 9 CODING NUCLEIC ACID MOLECULES, THE POLYPEPTIDE CODED, AND METHODS FORMING THEREOF
CA2447050A1 (en) 2001-05-14 2002-11-21 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
AU2002315525A1 (en) * 2001-07-03 2003-01-21 Dendreon San Diego Llc Nucleic acid molecules encoding a transmembrane serine protease 20, the encoded polypeptides and methods based thereon
MXPA04000397A (es) * 2001-07-19 2004-03-18 Yissum Res Dev Co Polipeptidos que tienen actividad catalitica de isomerasa de carotenoides, acidos nucleicos que los codifican y usos de los mismos.
WO2003044179A2 (en) * 2001-11-20 2003-05-30 Dendreon San Diego Llc Nucleic acid molecules encoding serine protease 17, the encoded polypeptides and methods based thereon
JP2007525981A (ja) 2004-03-03 2007-09-13 エフ．ホフマン−ラ　ロシュ　アーゲー 肝細胞成長因子のｎ末端フラグメントを組み換え発現するための方法
US7989181B2 (en) * 2006-04-22 2011-08-02 Scarab Genomics, Llc Methods and compositions for producing recombinant proteins using a gene for TRNA
JP4886654B2 (ja) * 2007-10-29 2012-02-29 日本ケミカルリサーチ株式会社 ヒト成長ホルモンの効率化された製造方法
KR101147860B1 (ko) 2008-09-22 2012-05-25 한국과학기술원 특정 아미노산의 ｔＲＮＡ와의 동시 발현을 통한 특정 아미노산 함량이 높은 단백질의 제조방법
KR101317420B1 (ko) * 2010-03-11 2013-10-10 한국과학기술원 고분자량의 재조합 실크 또는 실크 유사 단백질 및 이를 이용하여 제조된 마이크로 또는 나노 크기의 거미줄 또는 거미줄 유사 섬유

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4654307A (en) * 1983-02-17 1987-03-31 The Research Foundation Of State University Of New York Novel bacteria containing a plasmid having a tRNA code
SE455704B (sv) * 1983-10-24 1988-08-01 Ac Biotechnics Ab Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen
US5145776A (en) * 1987-01-14 1992-09-08 President & Fellows Of Harvard College Method of using T7 DNA polymerase to mutagenize and fill-in DNA
US4970147A (en) * 1988-03-17 1990-11-13 The General Hospital Corporation Oxygen regulatable gene expression

Also Published As

Publication number Publication date
AU618640B2 (en) 1992-01-02
CA2002516C (en) 1995-10-31
IL92264A (en) 1994-08-31
US6270988B1 (en) 2001-08-07
DK175456B1 (da) 2004-11-01
EP0373365B1 (de) 1994-05-18
IL92264A0 (en) 1990-07-26
JP2619077B2 (ja) 1997-06-11
AU4444389A (en) 1990-11-08
DK564789D0 (da) 1989-11-10
EP0373365A1 (de) 1990-06-20
DK564789A (da) 1990-05-12
CA2002516A1 (en) 1990-05-11
JPH02265490A (ja) 1990-10-30
ATE105863T1 (de) 1994-06-15
DE58907690D1 (de) 1994-06-23

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