IL160403A0 - Culture system for rapid expansion of human embryonic stem cells - Google Patents

Culture system for rapid expansion of human embryonic stem cells

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Publication number
IL160403A0
IL160403A0 IL16040302A IL16040302A IL160403A0 IL 160403 A0 IL160403 A0 IL 160403A0 IL 16040302 A IL16040302 A IL 16040302A IL 16040302 A IL16040302 A IL 16040302A IL 160403 A0 IL160403 A0 IL 160403A0
Authority
IL
Israel
Prior art keywords
stem cells
embryonic stem
human embryonic
culture system
rapid expansion
Prior art date
Application number
IL16040302A
Other languages
English (en)
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corp filed Critical Geron Corp
Publication of IL160403A0 publication Critical patent/IL160403A0/xx

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture

Families Citing this family (207)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US20100173410A1 (en) * 2000-03-09 2010-07-08 Wicell Research Institute, Inc. Cultivation of Primate Embryonic Stem Cells
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US6921665B2 (en) * 2000-11-27 2005-07-26 Roslin Institute (Edinburgh) Selective antibody targeting of undifferentiated stem cells
US6576464B2 (en) * 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
CN1599793A (zh) * 2001-12-07 2005-03-23 杰龙公司 人胚胎干细胞衍生的软骨细胞前体
GB0220145D0 (en) * 2002-08-30 2002-10-09 Thromb X Nv Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability
DE60239420D1 (de) * 2001-12-21 2011-04-21 Thrombogenics Nv Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen
US20040043482A1 (en) * 2002-01-04 2004-03-04 Kye-Hyung Paik Method of producing stem cell lines
AU2003303071B2 (en) 2002-12-16 2009-04-23 Technion Research & Development Foundation Ltd. Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
US7153650B2 (en) 2003-03-13 2006-12-26 Geron Corporation Marker system for preparing and characterizing high-quality human embryonic stem cells
US9592258B2 (en) 2003-06-27 2017-03-14 DePuy Synthes Products, Inc. Treatment of neurological injury by administration of human umbilical cord tissue-derived cells
US7820439B2 (en) * 2003-09-03 2010-10-26 Reliance Life Sciences Pvt Ltd. In vitro generation of GABAergic neurons from pluripotent stem cells
US20050054100A1 (en) * 2003-09-08 2005-03-10 Rennard Stephen I. Methods for fibroblast differentiation
AU2003289475A1 (en) * 2003-10-20 2005-05-05 Jms Co., Ltd. Cell handling device, human tissue regeneration composition, and human tissue regeneration method
US20050106725A1 (en) * 2003-11-19 2005-05-19 Palecek Sean P. Method of reducing cell differentiation
TWI280280B (en) * 2003-12-09 2007-05-01 Ind Tech Res Inst Culture system and method for expansion and undifferentiated growth of human embryonic stem cells
JP4632400B2 (ja) * 2003-12-16 2011-02-16 キヤノン株式会社 細胞培養用基板、その製造方法、それを用いた細胞スクリーニング法
US20060030042A1 (en) * 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
WO2005065354A2 (en) * 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
NZ548623A (en) 2004-01-02 2010-04-30 Advanced Cell Tech Inc Novel culture systems for ex vivo development of stem cells in telolecithal or eutelolecithal eggs
US20050186672A1 (en) * 2004-01-27 2005-08-25 Reliance Life Sciences Pvt. Ltd. Tissue system with undifferentiated stem cells derived from corneal limbus
KR20120091471A (ko) 2004-03-04 2012-08-17 도쿠리츠교세이호진 고쿠리츠간켄큐센터 래트 배아 줄기 세포
KR101178786B1 (ko) * 2004-03-23 2012-09-07 다이이찌 산쿄 가부시키가이샤 다능성 줄기세포의 증식 방법
US20080226726A1 (en) * 2004-03-24 2008-09-18 Jaconi Marisa E E 3D-Cardiac Tissue Engineering For the Cell Therapy of Heart Failure
JP5128946B2 (ja) * 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
US20050277124A1 (en) * 2004-06-10 2005-12-15 White Steven M Cardiac conduction system cells and uses thereof
JP2006000059A (ja) * 2004-06-17 2006-01-05 Two Cells Co Ltd 細胞外基質を用いた動物細胞の増殖及び分化促進方法
GB2431165B (en) * 2004-07-13 2009-04-01 Geron Corp Medium for growing human embryonic stem cells
DE102004043256B4 (de) * 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
ATE549395T1 (de) * 2004-09-08 2012-03-15 Wisconsin Alumni Res Found Medium und kultur von embryonalen stammzellen
AU2005282414C1 (en) * 2004-09-08 2011-04-07 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells
AU2005286097A1 (en) * 2004-09-21 2006-03-30 Nvr Labs. Ltd. Compositions and methods for stem cell expansion and differentiation
ES2800973T3 (es) * 2004-09-28 2021-01-07 Wisconsin Alumni Res Found Cultivo de células madre embrionarias de primates
US20070298016A1 (en) * 2004-09-29 2007-12-27 Nico Heins Methods For The Generation Of Hepatocyte-Like Cells From Human Blastocyst-Derived Stem (Hbs)
JP2006115767A (ja) * 2004-10-21 2006-05-11 Kyoto Univ フィーダー細胞の不在下で精原幹細胞を増殖させる方法
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
JP2008538276A (ja) * 2005-01-28 2008-10-23 ノヴァセラ・リミテッド 胚幹細胞培養のための方法
US20060263880A1 (en) * 2005-05-19 2006-11-23 Oakland University Protein factors supporting undifferentiated growth of human embryonic stem cells
JP2009500297A (ja) 2005-06-08 2009-01-08 セントカー・インコーポレーテツド 眼の変性についての細胞療法
AU2012203350B9 (en) * 2005-06-22 2015-11-26 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
JP5560393B2 (ja) 2005-06-22 2014-07-23 アステリアス バイオセラピューティクス インコーポレイテッド 心筋細胞系列細胞への霊長類多能性幹細胞の分化
JP5560391B2 (ja) 2005-06-22 2014-07-23 アステリアス バイオセラピューティクス インコーポレイテッド ヒト胚性幹細胞の懸濁培養法
CN101611142A (zh) * 2005-07-07 2009-12-23 综合医院公司 用于控制干细胞分化的方法
EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd MEDIA FOR BREEDING STEM CELLS
US7413900B2 (en) * 2005-10-31 2008-08-19 President And Fellows Of Harvard College Immortalized fibroblasts
EP1981971A4 (en) 2006-01-11 2009-08-12 Technion Res & Dev Foundation PROGENITORS OF CONNECTIVE TISSUES OBTAINED FROM ADULT STEM CELLS FOR TISSUE GENE
US8741643B2 (en) * 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US7964402B2 (en) * 2006-05-25 2011-06-21 Sanford-Burnham Medical Research Institute Methods for culture and production of single cell populations of human embryonic stem cells
US20080050814A1 (en) * 2006-06-05 2008-02-28 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
US20080064098A1 (en) * 2006-06-05 2008-03-13 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of maternal placental cells
EP2061871A1 (en) * 2006-09-14 2009-05-27 ProBioGen AG Modular culture system for maintenance, differentiation and proliferation of cells
JP2010507373A (ja) * 2006-10-24 2010-03-11 アデレード リサーチ アンド イノベイション ピーティーワイ エルティーディー 動物血清を含まない培地中で着床前胚から多能性細胞を単離するための方法
CA2679597A1 (en) * 2006-11-30 2008-06-05 Michael B. Dancu System and method for producing dynamic conditions including in vivo hemodynamic conditions in a three dimensional tubular structure
WO2008075378A2 (en) * 2006-12-19 2008-06-26 Stempeutics Research Private Limited Xeno-free culture conditions for human embryonic stem cells and methods thereof
WO2008075377A2 (en) * 2006-12-19 2008-06-26 Stempeutics Research Private Limited Germ lineage derived feeder cells and methods thereof
US8975068B2 (en) * 2007-01-25 2015-03-10 The General Hospital Corporation Isolated stem cell comprising a Xic flanking region transgene
EP2607477B1 (en) 2007-05-03 2020-09-23 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
DK2185693T3 (da) 2007-07-31 2019-09-23 Lifescan Inc Differentiering af humane embryoniske stamceller
CA2954431C (en) 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
US8372643B2 (en) * 2007-12-06 2013-02-12 Agency For Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
JP5733986B2 (ja) 2008-02-21 2015-06-10 ヤンセン バイオテツク,インコーポレーテツド 細胞の付着、培養、及び剥離のための方法、表面改質されたプレート、並びに組成物
CA2718438C (en) 2008-03-27 2021-06-29 Geron Corporation Differentiation of primate pluripotent stem cells to hematopoietic lineage cells
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
EP2942392B1 (en) 2008-06-30 2018-10-03 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
AU2009267167A1 (en) * 2008-06-30 2010-01-07 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
EP2350265B1 (en) 2008-10-31 2019-04-17 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
US9234178B2 (en) 2008-10-31 2016-01-12 Janssen Biotech, Inc. Differentiation of human pluripotent stem cells
KR101687344B1 (ko) 2008-11-20 2016-12-16 얀센 바이오테크 인코포레이티드 평면 기재상의 세포 부착 및 배양을 위한 방법 및 조성물
KR101774546B1 (ko) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 마이크로-캐리어 상의 만능 줄기 세포 배양
EP2362900A2 (en) * 2008-12-01 2011-09-07 Katholieke Universiteit Leuven, K.U. Leuven R&D MAINTENANCE/EXPANSION OF HSCs
EP3623374B1 (en) 2008-12-03 2021-09-08 The Scripps Research Institute Stem cell cultures
DE102009003439A1 (de) * 2009-02-05 2010-08-26 Cevec Pharmaceuticals Gmbh Neue permanente humane Zelllinie
WO2010096496A2 (en) 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
BRPI1008940A2 (pt) 2009-03-13 2020-10-27 Allergan, Inc. linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a
EP2233566A1 (en) 2009-03-17 2010-09-29 Vrije Universiteit Brussel Generation of pancreatic progenitor cells
US9487756B2 (en) 2009-03-20 2016-11-08 Mesoblast, Inc. Production of reprogrammed pluripotent cells
CN102482643B (zh) 2009-07-20 2016-06-29 詹森生物科技公司 人胚胎干细胞的分化
CN103952372B (zh) 2009-07-20 2016-10-05 詹森生物科技公司 人胚胎干细胞的分化
US8785184B2 (en) 2009-07-20 2014-07-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
PL2494035T3 (pl) * 2009-10-29 2018-07-31 Janssen Biotech, Inc. Pluripotencjalne komórki macierzyste
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011068879A2 (en) * 2009-12-02 2011-06-09 Research Development Foundation Selection of stem cell clones with defined differentiation capabilities
SG10201408552YA (en) * 2009-12-23 2015-02-27 Janssen Biotech Inc Differentiation of human embryonic stem cells
CN102712902B (zh) * 2009-12-23 2019-01-08 詹森生物科技公司 人胚胎干细胞的分化
PH12012501686A1 (en) 2010-03-01 2020-10-19 Janssen Biotech Inc Methods for purifying cells derived from pluripotent stem cells
US9029145B2 (en) 2010-04-08 2015-05-12 The University Court Of The University Of Edinburgh Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof
US8802438B2 (en) 2010-04-16 2014-08-12 Children's Medical Center Corporation Compositions, kits, and methods for making induced pluripotent stem cells using synthetic modified RNAs
RU2587634C2 (ru) 2010-05-12 2016-06-20 Янссен Байотек, Инк. Дифференцирование эмбриональных стволовых клеток человека
US8513015B2 (en) 2010-06-11 2013-08-20 Corning Incorporated Laminin-entactin complex and cell culture article and methods thereof
WO2011159684A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
EP2582793B1 (en) 2010-06-15 2017-09-06 Cellular Dynamics International, Inc. A compendium of ready-built stem cell models for interrogation of biological response
WO2012006440A2 (en) 2010-07-07 2012-01-12 Cellular Dynamics International, Inc. Endothelial cell production by programming
EP2593117B1 (en) 2010-07-12 2019-03-20 University of Southern California Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
EP2606128B1 (de) * 2010-08-16 2014-09-10 CEVEC Pharmaceuticals GmbH Permanente humane amniozyten zellinie zur herstellung von influenzaviren
CN103154237B (zh) 2010-08-31 2016-03-16 詹森生物科技公司 多能干细胞的分化
PL2853589T3 (pl) 2010-08-31 2018-05-30 Janssen Biotech, Inc Różnicowanie ludzkich zarodkowych komórek macierzystych
EP3372672A1 (en) 2010-08-31 2018-09-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6283223B2 (ja) 2010-09-07 2018-02-21 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド 多能性幹細胞を培養するための新規な方法および培養培地
JP2013545439A (ja) 2010-09-17 2013-12-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 多能性幹細胞の有用性および安全性の特徴決定を行うための機能的ゲノミクスアッセイ
WO2012048276A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
KR20140004707A (ko) 2011-01-31 2014-01-13 독립행정법인 국립국제의료연구 센터 다능성 줄기 세포 유래 고기능 간세포와 그의 제조 방법 및 약제 대사 독성 시험 방법
CA2826386C (en) 2011-02-08 2020-04-28 FUJIFILM Cellular Dynamics, Inc. Hematopoietic precursor cell production by programming
WO2012135253A1 (en) 2011-03-29 2012-10-04 Geron Corporation Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
US10478206B2 (en) 2011-04-29 2019-11-19 University Of Southern California Instruments and methods for the implantation of cell-seeded substrates
US8877489B2 (en) 2011-12-05 2014-11-04 California Institute Of Technology Ultrathin parylene-C semipermeable membranes for biomedical applications
WO2013010965A1 (en) 2011-07-15 2013-01-24 Universite Libre De Bruxelles Generation of mesodermal cells from pluripotent stem cells
CN102952780A (zh) * 2011-08-29 2013-03-06 北京清美联创干细胞科技有限公司 一种培养嗅鞘细胞的培养基
EP3693455A1 (en) 2011-11-04 2020-08-12 Memorial Sloan-Kettering Cancer Center Midbrain dopamine (da) neurons for engraftment
IL287381B2 (en) 2011-11-14 2024-04-01 Astellas Inst For Regenerative Medicine Pharmaceutical preparations of human rpe cells and uses thereof
JP5999658B2 (ja) * 2011-11-25 2016-09-28 国立大学法人京都大学 多能性幹細胞の培養方法
US9248013B2 (en) 2011-12-05 2016-02-02 California Institute Of Technology 3-Dimensional parylene scaffold cage
FI2794857T3 (fi) 2011-12-22 2026-03-25 Janssen Biotech Inc Ihmisen alkion kantasolujen erilaistaminen yksittäisiksi hormonaalisiksi insuliinipositiivisiksi soluiksi
US9828583B2 (en) 2012-01-13 2017-11-28 The General Hospital Corporation Isolated human lung progenitor cells and uses thereof
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
WO2013134015A2 (en) 2012-03-05 2013-09-12 Wake Forest University Health Sciences Non-viral episomal suicide construct
PT2822936T (pt) 2012-03-07 2021-12-02 Univ Of Delhi Derivados da aminoquinolina e suas utilizações
CA2866590A1 (en) 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
EP3450544A1 (en) 2012-06-08 2019-03-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endodrine cells
JP2014082956A (ja) 2012-10-19 2014-05-12 Somar Corp 細胞培養基材、およびそれを用いた細胞培養方法並びに多能性幹細胞の分化誘導方法
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
DK2938723T3 (da) 2012-12-31 2023-02-20 Janssen Biotech Inc Differentiering af humane embryonale stamceller til pancreatiske endokrine celler under anvendelse af hb9-regulatorer
BR112015015714A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas
ES2837763T3 (es) 2012-12-31 2021-07-01 Janssen Biotech Inc Cultivo de células madre embrionarias humanas en la interconexión aire-líquido para la diferenciación en células endocrinas pancreáticas
CN105229144A (zh) 2013-02-22 2016-01-06 细胞动力学国际有限公司 通过组合的遗传工程和化学工程经由正向编程产生肝细胞
AU2014248167B2 (en) 2013-04-03 2019-10-10 FUJIFILM Cellular Dynamics, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
EP3498824A1 (en) 2013-04-26 2019-06-19 Memorial Sloan-Kettering Cancer Center Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
CN120796168A (zh) 2013-06-11 2025-10-17 哈佛学院校长同事会 SC-β细胞以及用于产生其的组合物和方法
WO2015073913A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
CA2930877A1 (en) 2013-11-18 2015-05-21 Crispr Therapeutics Ag Crispr-cas system materials and methods
AU2014352773B2 (en) 2013-11-21 2021-04-29 Memorial Sloan-Kettering Cancer Center Specification of functional cranial placode derivatives from human pluripotent stem cells
EP3835419A1 (en) 2013-12-12 2021-06-16 The Regents of The University of California Methods and compositions for modifying a single stranded target nucleic acid
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
US20170107486A1 (en) 2014-04-21 2017-04-20 Cellular Dynamics International, Inc. Hepatocyte production via forward programming by combined genetic and chemical engineering
WO2015175307A1 (en) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
US20170114323A1 (en) 2014-06-19 2017-04-27 Whitehead Institute For Biomedical Research Uses of kinase inhibitors for inducing and maintaining pluripotency
US11001803B2 (en) 2014-07-31 2021-05-11 Association Francaise Contre Les Myopathies Method for generating neuronal and muscular cells
EP3188763B1 (en) 2014-09-02 2020-05-13 The Regents of The University of California Methods and compositions for rna-directed target dna modification
EP3198006B1 (en) 2014-09-26 2021-03-24 Terumo BCT, Inc. Scheduled feed
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
EP3234110B1 (en) 2014-12-18 2024-02-28 President and Fellows of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED ß CELLS AND USES THEREOF
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US10384207B2 (en) 2015-07-21 2019-08-20 Neuro Probe Incorporated Assay apparatus and methods
US20190048340A1 (en) 2015-09-24 2019-02-14 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
IL242380A0 (en) 2015-10-29 2016-02-01 Yeda Res & Dev A method of inducing cardiomyocytes proliferation and treating heart diseases
KR102211270B1 (ko) 2016-01-14 2021-02-04 디퍼이 신테스 프로덕츠, 인코포레이티드 Hutc의 동결보존을 위한 조성물 및 방법
US11674952B2 (en) 2016-02-24 2023-06-13 The Rockefeller University Embryonic cell-based therapeutic candidate screening systems, models for Huntington's Disease and uses thereof
WO2017173064A1 (en) 2016-03-30 2017-10-05 Asterias Biotherapeutics, Inc. Oligodendrocyte progenitor cell compositions
US10420803B2 (en) 2016-04-14 2019-09-24 Janssen Biotech, Inc. Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
EP3472321A2 (en) 2016-06-17 2019-04-24 Genesis Technologies Limited Crispr-cas system, materials and methods
AU2017342176A1 (en) 2016-10-10 2019-05-02 The National Institute for Biotechnology in the Negev Ltd. Non-cytotoxic modified cells and use thereof
US10828330B2 (en) 2017-02-22 2020-11-10 IO Bioscience, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
US12440578B2 (en) 2017-02-22 2025-10-14 Io Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
CN117247899A (zh) 2017-03-31 2023-12-19 泰尔茂比司特公司 细胞扩增
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
US12234441B2 (en) 2017-03-31 2025-02-25 Terumo Bct, Inc. Cell expansion
CN107523544B (zh) * 2017-10-16 2020-03-31 中国人民解放军第三军医大学第一附属医院 一种肿瘤细胞球的制备方法和应用
EP3707155A2 (en) 2017-11-09 2020-09-16 Vertex Pharmaceuticals Incorporated Crispr/cas systems for treatment of dmd
EP3710021A4 (en) 2017-11-15 2021-08-11 Semma Therapeutics, Inc. ISLAND CELL MANUFACTURING COMPOSITIONS AND METHOD OF USE
WO2019106680A1 (en) 2017-12-03 2019-06-06 Yeda Research And Development Co. Ltd. Treatment of an ischemic heart disease
JP2021506251A (ja) 2017-12-14 2021-02-22 クリスパー セラピューティクス アーゲー 新規rnaプログラム可能エンドヌクレアーゼ系、ならびにゲノム編集および他の適用におけるその使用
MA52074A (fr) 2018-03-19 2021-01-27 Bayer Healthcare Llc Nouveaux systèmes d'endonucléase à arn programmable et leurs utilisations
WO2019213276A1 (en) 2018-05-02 2019-11-07 Novartis Ag Regulators of human pluripotent stem cells and uses thereof
EP3572512A1 (en) 2018-05-24 2019-11-27 B.R.A.I.N. Ag A method for engineering a protein
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
CN113710799B (zh) 2018-11-28 2024-11-12 克里斯珀医疗股份公司 用于在LNP中使用的编码CAS9的优化mRNA
KR20260020476A (ko) 2019-01-23 2026-02-11 아스테리아스 바이오세라퓨틱스, 인크. 인간 만능 줄기 세포로부터의 배측-유래된 희소돌기아교세포 전구 세포
MX2021010559A (es) 2019-03-07 2021-12-15 Univ California Polipéptidos efectores de crispr-cas y métodos de uso de estos.
WO2020209959A1 (en) 2019-03-08 2020-10-15 Crispr Therapeutics Ag Nucleobase-editing fusion protein systems, compositions, and uses thereof
SG11202109741VA (en) 2019-03-12 2021-10-28 Crispr Therapeutics Ag Novel high fidelity rna-programmable endonuclease systems and uses thereof
US20210047649A1 (en) 2019-05-08 2021-02-18 Vertex Pharmaceuticals Incorporated Crispr/cas all-in-two vector systems for treatment of dmd
US20220411759A1 (en) 2019-05-21 2022-12-29 President And Fellows Of Harvard College Endocrine differentiation-inducing molecule
CA3144948A1 (en) 2019-06-25 2020-12-30 Vertex Pharmaceuticals Incorporated Enhanced differentiation of beta cells
EP3772538A1 (en) * 2019-08-09 2021-02-10 Urosphere Method for differentiating epithelial stem cells
WO2021030424A1 (en) 2019-08-13 2021-02-18 Semma Therapeutics, Inc. Pancreatic differentiation
JP7701348B2 (ja) 2019-09-20 2025-07-01 ベイジン・インスティチュート・フォー・ステム・セル・アンド・リジェネラティヴ・メディスン 多能性幹細胞、医薬組成物、並びにそれらの調製方法及びそれらの適用
US20220412937A1 (en) * 2019-11-27 2022-12-29 Symrise Ag Device and Method for the Analytical and Sensory Determination of the Release of an Active Substance from a Release System
CN111117959A (zh) * 2020-01-08 2020-05-08 山东龙辰生物技术有限公司 Dc-cik细胞培养基、dc-cik细胞的培养方法及应用
US20230235319A1 (en) 2020-06-12 2023-07-27 Bayer Aktiengesellschaft Crispr-cas12a directed random mutagenesis agents and methods
WO2022026932A2 (en) 2020-07-31 2022-02-03 Vertex Pharmaceuticals Incorporated Differentiation of pancreatic endocrine cells
EP4314244B1 (en) 2021-03-23 2025-07-23 Terumo BCT, Inc. Cell capture and expansion
US20250188417A1 (en) 2021-04-11 2025-06-12 President And Fellows Of Harvard College Cardiomyocytes and compositions and methods for producing the same
CN117242173A (zh) 2021-05-03 2023-12-15 安斯泰来再生医药协会 产生成熟角膜内皮细胞的方法
WO2022235869A1 (en) 2021-05-07 2022-11-10 Astellas Institute For Regenerative Medicine Methods of generating mature hepatocytes
EP4101928A1 (en) 2021-06-11 2022-12-14 Bayer AG Type v rna programmable endonuclease systems
AU2022290382A1 (en) 2021-06-11 2023-11-23 Bayer Aktiengesellschaft Type v rna programmable endonuclease systems
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
EP4144841A1 (en) 2021-09-07 2023-03-08 Bayer AG Novel small rna programmable endonuclease systems with impoved pam specificity and uses thereof
JP7798323B2 (ja) * 2021-09-08 2026-01-14 公立大学法人秋田県立大学 遺伝子組み換え細胞製造方法、培養方法、遺伝子組み換え細胞、発現ベクター製造方法、及び発現ベクター
JP2024540987A (ja) 2021-10-21 2024-11-06 バーテックス ファーマシューティカルズ インコーポレイテッド 低免疫細胞
CA3232971A1 (en) 2021-11-01 2023-05-04 George Harb Stem cell derived pancreatic islet differentiation
EP4453191A1 (en) 2021-12-23 2024-10-30 Bayer Aktiengesellschaft Novel small type v rna programmable endonuclease systems
WO2023129418A1 (en) * 2021-12-30 2023-07-06 Atelier Meats Corp. Nonhuman stem cells and their use for production of cultured meat
US12209689B2 (en) 2022-02-28 2025-01-28 Terumo Kabushiki Kaisha Multiple-tube pinch valve assembly
CN119403922A (zh) 2022-06-10 2025-02-07 拜耳公司 新的小型v型rna可编程内切核酸酶系统
TW202417614A (zh) 2022-08-23 2024-05-01 安斯泰來再生醫藥協會 用於治療眼部病症的感光救援細胞(prc)組成物及方法
USD1099116S1 (en) 2022-09-01 2025-10-21 Terumo Bct, Inc. Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device
WO2025126208A1 (en) 2023-12-12 2025-06-19 Accellta Ltd. Media for maintaining, differentiating, or both, pluripotent stem cells
WO2026039350A1 (en) 2024-08-13 2026-02-19 Isp Investments Llc A lactam moiety based cryoprotectant, compositions thereof and a method of cryopreservation of biological materials
WO2026069163A1 (en) 2024-09-25 2026-04-02 Crispr Therapeutics Ag Differentiation method for producing immature beta cells

Family Cites Families (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4256042A (en) * 1979-10-04 1981-03-17 Miner Enterprises, Inc. Railway hopper car sliding gate lock
US4282366A (en) * 1979-11-06 1981-08-04 International Paper Company Organosilicon quaternary ammonium antimicrobial compounds
US4423145A (en) 1981-05-07 1983-12-27 Stampfer Martha R Enhanced growth medium and method for culturing human mammary epithelial cells
US4829000A (en) * 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US5104795A (en) * 1985-11-13 1992-04-14 Zoma Corporation Shortened phosphoglycerate kinase promoter
GB2197915B (en) 1986-11-19 1990-11-14 Rolls Royce Plc Improvements in or relating to fluid bearings
US6022742A (en) 1986-11-26 2000-02-08 Kopf; Henry B. Culture device and method
AU623922B2 (en) 1988-08-04 1992-05-28 Amrad Operations Pty. Limited In vitro propagation of embryonic stem cells
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5411883A (en) 1989-12-26 1995-05-02 Somatix Therapy Corporation Proliferated neuron progenitor cell product and process
US5635387A (en) * 1990-04-23 1997-06-03 Cellpro, Inc. Methods and device for culturing human hematopoietic cells and their precursors
US5614396A (en) * 1990-06-14 1997-03-25 Baylor College Of Medicine Methods for the genetic modification of endogenous genes in animal cells by homologous recombination
US5981165A (en) * 1991-07-08 1999-11-09 Neurospheres Holdings Ltd. In vitro induction of dopaminergic cells
US5851832A (en) 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
WO1993003142A1 (en) 1991-08-07 1993-02-18 Albert Einstein College Of Medicine Proliferation of hepatocyte precursors
JPH05252941A (ja) * 1991-08-12 1993-10-05 Sakai Enetsukusu Kk 動物細胞培養用担体
US5332672A (en) 1991-12-02 1994-07-26 Regeneron Pharmaceuticals, Inc. Prevention of ES cell differentiation by ciliary neurotrophic factor
WO1994000470A1 (en) * 1992-06-30 1994-01-06 The Wistar Institute Of Anatomy And Biology DEVELOPMENTAL EMBRYONIC MOUSE cDNA LIBRARIES
US5840484A (en) * 1992-07-17 1998-11-24 Incyte Pharmaceuticals, Inc. Comparative gene transcript analysis
US5849553A (en) 1992-07-27 1998-12-15 California Institute Of Technology Mammalian multipotent neural stem cells
US5672499A (en) * 1992-07-27 1997-09-30 California Institute Of Technology Immoralized neural crest stem cells and methods of making
WO1994007997A1 (en) 1992-10-06 1994-04-14 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Long-term proliferation of primordial germ cells
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5766948A (en) 1993-01-06 1998-06-16 The Regents Of The University Of California Method for production of neuroblasts
US5362642A (en) 1993-02-10 1994-11-08 Hyclone Laboratories Methods and containment system for storing, reconstituting, dispensing and harvesting cell culture media
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5405772A (en) 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
US5578475A (en) 1993-07-12 1996-11-26 Life Technologies, Inc. Composition and methods for transfecting eukaryotic cells
US6686198B1 (en) 1993-10-14 2004-02-03 President And Fellows Of Harvard College Method of inducing and maintaining neuronal cells
US5643761A (en) * 1993-10-27 1997-07-01 The Trustees Of Columbia University In The City Of New York Method for generating a subtracted cDNA library and uses of the generated library
US5591625A (en) * 1993-11-24 1997-01-07 Case Western Reserve University Transduced mesenchymal stem cells
US5534404A (en) 1993-12-10 1996-07-09 Cytotherapeutics, Inc. Glucose responsive insulin secreting β-cell lines and method for producing same
US5639618A (en) 1994-05-13 1997-06-17 Plurion, Inc. Method of isolating a lineage specific stem cell in vitro
US5583016A (en) 1994-07-07 1996-12-10 Geron Corporation Mammalian telomerase
US5914268A (en) 1994-11-21 1999-06-22 National Jewish Center For Immunology & Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5874301A (en) 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
CA2207216A1 (en) 1994-12-09 1996-06-13 Joseph D. Mosca System for the maintenance, growth and differentiation of human and non-human primate pluripotent stem, progenitor and mature bone marrow cells
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
DE59610339D1 (de) * 1995-02-16 2003-05-22 Forschungszentrum Juelich Gmbh Verfahren zur Kultivierung von Organfunktionszellen
US5919449A (en) 1995-05-30 1999-07-06 Diacrin, Inc. Porcine cardiomyocytes and their use in treatment of insufficient cardiac function
US6051429A (en) 1995-06-07 2000-04-18 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
US6238908B1 (en) 1995-06-07 2001-05-29 Aastrom Biosciences, Inc. Apparatus and method for maintaining and growth biological cells
US5968506A (en) * 1995-08-04 1999-10-19 Geron Corporation Purified telomerase
WO1997018298A1 (en) 1995-11-14 1997-05-22 Regents Of The University Of Minnesota Ex vivo culture of stem cells
FR2744133A1 (fr) 1996-01-30 1997-08-01 Imedex Procede de culture de cellules a potentiel angiogenique pour induire la morphogenese vasculaire desdites cellules, modeles in vitro de morphogenese vasculaire ainsi obtenus, et leurs applications notamment au criblage de drogues
CA2246712A1 (en) 1996-02-16 1997-08-21 The University Of Edinburgh Cytokine expressed by dia/lif-deficient embryonic stem cells for the inhibition of differentiation
US5753506A (en) * 1996-05-23 1998-05-19 Cns Stem Cell Technology, Inc. Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
AU3392697A (en) 1996-06-14 1998-01-07 Regents Of The University Of California, The (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
US5856136A (en) 1996-07-03 1999-01-05 Incyte Pharmaceuticals, Inc. Human stem cell antigens
WO1998030678A1 (en) 1997-01-07 1998-07-16 Steindler Dennis A Isolated mammalian neural stem cells, methods of making such cells, and methods of using such cells
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
US6361996B1 (en) 1997-05-07 2002-03-26 University Of Utah Research Foundation Neuroepithelial stem cells and glial-restricted intermediate precursors
DE19727962A1 (de) 1997-07-02 1999-01-14 Juergen Hescheler Fluoreszierende Proteine als zelltypspezifische Reporter
US6235527B1 (en) 1997-11-29 2001-05-22 University Of Utah Research Foundation Lineage restricted glial precursors from the central nervous system
JP4371179B2 (ja) 1997-07-04 2009-11-25 ユニバーシティー オブ ユタ リサーチ ファンデーション 系列限定ニューロン前駆体
US6387369B1 (en) 1997-07-14 2002-05-14 Osiris Therapeutics, Inc. Cardiac muscle regeneration using mesenchymal stem cells
US7144997B2 (en) 1997-07-24 2006-12-05 Curis, Inc. Vertebrate embryonic patterning-inducing proteins, compositions and uses related therto
WO1999010535A1 (en) 1997-08-22 1999-03-04 Yale University A process to study changes in gene expression in stem cells
US5968829A (en) 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
CA2307807C (en) * 1997-10-23 2008-09-02 Andrea G. Bodnar Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
WO1999042122A1 (en) 1998-02-19 1999-08-26 University Of Southern California Method of promoting embryonic stem cell proliferation
AU757036B2 (en) 1998-02-27 2003-01-30 Medical College Of Hampton Roads, The Derivation of cells and tissues from embryonic pre-stem cells for transplantation therapies
CA2324591A1 (en) 1998-04-09 1999-10-21 Bresagen Limited Cell differentiation/proliferation and maintenance factor and uses thereof
AU755849B2 (en) 1998-09-22 2002-12-19 Neuralstem Biopharmaceuticals, Ltd. Stable neural stem cell lines
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US7413904B2 (en) * 1998-10-23 2008-08-19 Geron Corporation Human embryonic stem cells having genetic modifications
US6875607B1 (en) * 1998-11-09 2005-04-05 Es Cell International Pte Ltd Embryonic stem cells
US6372494B1 (en) 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions
AU782286B2 (en) 1999-06-30 2005-07-14 Advanced Cell Technology, Inc. Cytoplasmic transfer to de-differentiate recipient cells
CA2382859A1 (en) * 1999-10-07 2001-04-12 Genentech, Inc. Novel polypeptides, their nucleic acids, and methods for their use in angiogenesis and vascularization
US6280718B1 (en) * 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
CA2397292A1 (en) 2000-01-14 2001-07-19 Bresagen Limited Cell production
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US7282366B2 (en) 2000-04-27 2007-10-16 Geron Corporation Hepatocytes for therapy and drug screening made from embryonic stem cells
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US7256042B2 (en) 2000-04-27 2007-08-14 Geron Corporation Process for making hepatocytes from pluripotent stem cells
US7473555B2 (en) * 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
US7250294B2 (en) 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
WO2001088104A2 (en) * 2000-05-17 2001-11-22 Geron Corporation Neural progenitor cell populations
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
US6921665B2 (en) * 2000-11-27 2005-07-26 Roslin Institute (Edinburgh) Selective antibody targeting of undifferentiated stem cells
IL159578A0 (en) 2001-07-06 2004-06-01 Geron Corp Messenchymal cells and osteoblasts from human embryonic stem cell
WO2003006950A2 (en) 2001-07-12 2003-01-23 Geron Corporation Cells of the cardiomyocyte lineage produced from human pluripotent stem cells
WO2003038070A1 (en) 2001-10-31 2003-05-08 Asahi Kasei Kabushiki Kaisha Base material for culturing embryo stem cells and culture method
IL162130A0 (en) * 2001-12-07 2005-11-20 Robarts Res Inst Hematopoietic cells from human embryonic stem cells
IL162131A0 (en) 2001-12-07 2005-11-20 Geron Corp Islet cells from human embryonic stem cells
CN1599793A (zh) 2001-12-07 2005-03-23 杰龙公司 人胚胎干细胞衍生的软骨细胞前体
US20030113910A1 (en) * 2001-12-18 2003-06-19 Mike Levanduski Pluripotent stem cells derived without the use of embryos or fetal tissue
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
AU2003303071B2 (en) 2002-12-16 2009-04-23 Technion Research & Development Foundation Ltd. Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
AU2004278634B2 (en) 2003-10-03 2009-10-01 Keiichi Fukuda Method of inducing the differentiation of stem cells into cardiomyocytes
WO2005065354A2 (en) * 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
JP4971131B2 (ja) 2004-03-19 2012-07-11 ジェロン・コーポレーション 再生医学に適した高純度心筋細胞調製物を作製するための方法
JP5128946B2 (ja) 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
EP1844136B1 (en) 2004-12-29 2014-08-27 Hadasit Medical Research Services And Development Ltd. Stem cells culture systems
WO2008004990A2 (en) 2006-07-06 2008-01-10 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
DK3441459T3 (da) 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture

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