AU3392697A - (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof - Google Patents

(in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof

Info

Publication number
AU3392697A
AU3392697A AU33926/97A AU3392697A AU3392697A AU 3392697 A AU3392697 A AU 3392697A AU 33926/97 A AU33926/97 A AU 33926/97A AU 3392697 A AU3392697 A AU 3392697A AU 3392697 A AU3392697 A AU 3392697A
Authority
AU
Australia
Prior art keywords
derivation
vitro
culture
stem cells
pluripotent stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU33926/97A
Inventor
Roger A. Pedersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Publication of AU3392697A publication Critical patent/AU3392697A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
AU33926/97A 1996-06-14 1997-06-13 (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof Abandoned AU3392697A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US66521796A 1996-06-14 1996-06-14
US08665217 1996-06-14
PCT/US1997/010316 WO1997047734A1 (en) 1996-06-14 1997-06-13 In vitro derivation and culture of primate pluripotent stem cells and therapeutic uses thereof

Publications (1)

Publication Number Publication Date
AU3392697A true AU3392697A (en) 1998-01-07

Family

ID=24669212

Family Applications (1)

Application Number Title Priority Date Filing Date
AU33926/97A Abandoned AU3392697A (en) 1996-06-14 1997-06-13 (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof

Country Status (2)

Country Link
AU (1) AU3392697A (en)
WO (1) WO1997047734A1 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271436B1 (en) 1996-10-11 2001-08-07 The Texas A & M University System Cells and methods for the generation of transgenic pigs
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
WO2000034525A1 (en) * 1998-12-09 2000-06-15 Vistagen, Inc. Toxicity typing using embryoid bodies
US6635802B1 (en) 2000-01-10 2003-10-21 The Texas A&M University System Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US7473555B2 (en) 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
US20020012905A1 (en) 2000-06-14 2002-01-31 Snodgrass H. Ralph Toxicity typing using liver stem cells
US8153424B2 (en) 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US7588937B2 (en) 2001-10-03 2009-09-15 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US6887706B2 (en) 2001-10-03 2005-05-03 Wisconsin Alumni Research Foundation Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells
EP1446476A4 (en) 2001-11-02 2005-01-26 Wisconsin Alumni Res Found Endothelial cells derived from primate embryonic stem cells
MXPA04008824A (en) 2002-03-15 2005-06-17 Wicell Res Inst Inc Method for generating primate trophoblasts.
WO2007002086A2 (en) 2005-06-22 2007-01-04 Geron Corporation Suspension culture of human embryonic stem cells
WO2007143117A2 (en) 2006-06-02 2007-12-13 Geron Corporation Differentiation of primate pluripotent cells to hepatocyte-lineage cells
WO2008049161A1 (en) * 2006-10-24 2008-05-02 Adelaide Research & Innovation Pty Ltd Method for the isolation of pluripotent cells from a pre-implantation embryo in a culture medium free from animal serum

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0380646B1 (en) * 1988-08-04 1999-01-20 Amrad Corporation Limited Use of leukaemia inhibitory factor (lif) for the (in vitro) propagation of embryonic stem cells
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
WO1995016770A1 (en) * 1993-12-17 1995-06-22 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells

Also Published As

Publication number Publication date
WO1997047734A1 (en) 1997-12-18

Similar Documents

Publication Publication Date Title
AU3392697A (en) (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
AU1644295A (en) Cell culture substrates and methods of use
EP0812201A4 (en) -i(IN VITRO) AMPLIFICATION OF STEM CELLS
AU2730497A (en) Cryopreservation and extensive subculturing of human mesenchymal stem cells
AU5566396A (en) Manipulation of plant cell and tissue cultures
AU9582398A (en) Methods and devices for the long-term culture of hematopoietic progenitor cells
EP1650307A3 (en) Methods for making recombinant proteins using apoptosis inhibitors
EP1398370A3 (en) In-vitro micro-organs
AU2001226395A1 (en) Techniques for growth and differentiation of human pluripotent stem cells
AU1119397A (en) Ex vivo culture of stem cells
AU4336599A (en) (in vitro) maintenance of hematopoietic stem cells
WO1999011758A3 (en) Cultures of human cns neural stem cells
AU7832194A (en) Gas permeable bioreactor and method of use
GB9213970D0 (en) Improvements in and relating to tissue culture vessels
AU7769887A (en) Apparatus and methods of culturing motile microorganisms
AU3783993A (en) Culturing of hematopoietic stem cells and their genetic engineering
AU3557597A (en) The isolation and culture of fetal cells from peripheral maternal blood
AU1478000A (en) (in utero) transplantation of human mesenchymal stem cells
AU7468396A (en) In vitro growth of functional islets of langerhans and in vivo uses thereof
PL322271A1 (en) Novel defined mixtures of enzymes for use in growing cells and healing wounds
AU4378996A (en) Cell compositions for use in transplantation and ex vivo gene therapy
AU2002230459A1 (en) Cell culture apparatus and methods of use
AU2001217686A1 (en) Method of establishing cultures of human dendritic cells and use thereof
AU2998300A (en) Genetically altered mesenchymal stem cells and methods of use thereof
AU7954500A (en) Method of in vitro culture of lymphocytes and gene therapy compositions