DE60239420D1 - Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen - Google Patents
Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellenInfo
- Publication number
- DE60239420D1 DE60239420D1 DE60239420T DE60239420T DE60239420D1 DE 60239420 D1 DE60239420 D1 DE 60239420D1 DE 60239420 T DE60239420 T DE 60239420T DE 60239420 T DE60239420 T DE 60239420T DE 60239420 D1 DE60239420 D1 DE 60239420D1
- Authority
- DE
- Germany
- Prior art keywords
- culture
- stem cells
- cells
- nobacco
- embryoonic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000130 stem cell Anatomy 0.000 title abstract 3
- 230000005540 biological transmission Effects 0.000 title 1
- 238000000338 in vitro Methods 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 102000004058 Leukemia inhibitory factor Human genes 0.000 abstract 2
- 108090000581 Leukemia inhibitory factor Proteins 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000001671 embryonic stem cell Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000002243 precursor Substances 0.000 abstract 2
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract 1
- 102000028677 Rab9 Human genes 0.000 abstract 1
- 108050007276 Rab9 Proteins 0.000 abstract 1
- 210000002459 blastocyst Anatomy 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 210000004602 germ cell Anatomy 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000003394 haemopoietic effect Effects 0.000 abstract 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 abstract 1
- 238000002513 implantation Methods 0.000 abstract 1
- 210000001161 mammalian embryo Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5415—Leukaemia inhibitory factor [LIF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/51—Umbilical cord; Umbilical cord blood; Umbilical stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2306—Interleukin-6 (IL-6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Biochemistry (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Hematology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/BE2001/000221 WO2003054169A1 (en) | 2001-12-21 | 2001-12-21 | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability |
| GBGB0220145.7A GB0220145D0 (en) | 2002-08-30 | 2002-08-30 | Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability |
| PCT/BE2002/000196 WO2003054170A1 (en) | 2001-12-21 | 2002-12-20 | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability and for the culture of adult stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60239420D1 true DE60239420D1 (de) | 2011-04-21 |
Family
ID=25661295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60239420T Expired - Lifetime DE60239420D1 (de) | 2001-12-21 | 2002-12-20 | Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8697444B2 (de) |
| EP (1) | EP1458852B1 (de) |
| JP (1) | JP4408371B2 (de) |
| CN (1) | CN100526455C (de) |
| AT (1) | ATE501167T1 (de) |
| AU (1) | AU2002357410A1 (de) |
| DE (1) | DE60239420D1 (de) |
| HK (1) | HK1075276A1 (de) |
| WO (1) | WO2003054170A1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE501167T1 (de) | 2001-12-21 | 2011-03-15 | Thrombogenics Nv | Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen |
| AU2006286149B2 (en) | 2005-08-29 | 2012-09-13 | Technion Research And Development Foundation Ltd. | Media for culturing stem cells |
| EP2059586B1 (de) | 2006-08-02 | 2016-07-20 | Technion Research & Development Foundation Ltd. | Verfahren zur expansion embryonaler stammzellen in einer suspensionskultur |
| CA3174901A1 (en) | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Ltd. | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| GB201122047D0 (en) * | 2011-12-21 | 2012-02-01 | Kymab Ltd | Transgenic animals |
| PL2809150T3 (pl) | 2012-02-01 | 2020-04-30 | Regeneron Pharmaceuticals, Inc. | Humanizowane myszy wykazujące ekspresję łańcuchów ciężkich zawierających domeny VL |
| WO2015070113A1 (en) * | 2013-11-09 | 2015-05-14 | Mice With Horns, Llc | Materials and methods for making a recessive gene dominant |
| CN106635980B (zh) * | 2016-11-16 | 2019-09-24 | 中国农业大学 | 一种自发永生化的绵羊外周血来源细胞系及其建立方法 |
| CN113215097B (zh) * | 2021-06-24 | 2023-03-24 | 成都中医药大学 | 一种高比例b7h4阳性脐带间充质干细胞、其培养方法及培养液 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT87133B (pt) | 1987-04-02 | 1992-07-31 | Amrad Corp Ltd | Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif |
| US5166065A (en) | 1988-08-04 | 1992-11-24 | Amrad Corporation Limited | In vitro propagation of embryonic stem cells |
| EP0453453B1 (de) * | 1989-01-10 | 1996-09-11 | Amrad Corporation Limited | Leukämiehemmender faktor aus vieharten und seine verwendung zur verbesserung der implantation und der entwicklung von embryonalen zellen |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| WO1997020035A1 (en) * | 1995-11-29 | 1997-06-05 | Utah State University | Establishment, maintenance, and transfection of totipotent embryonic stem cells from the embryos of domestic animals |
| JPH1052263A (ja) | 1996-05-21 | 1998-02-24 | Rikagaku Kenkyusho | 胚性幹細胞 |
| US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| US6458589B1 (en) * | 2000-04-27 | 2002-10-01 | Geron Corporation | Hepatocyte lineage cells derived from pluripotent stem cells |
| US20040053406A1 (en) | 2000-06-28 | 2004-03-18 | Luc Schoonjans | Pluripotent embryonic stem (es) cell lines, improved methods for their production, and their use for germ line transmission and for the generation of genetically modified animals |
| AU2002218893A1 (en) | 2001-12-21 | 2003-07-09 | Thromb-X Nv | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability |
| ATE501167T1 (de) | 2001-12-21 | 2011-03-15 | Thrombogenics Nv | Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen |
| GB0220145D0 (en) | 2002-08-30 | 2002-10-09 | Thromb X Nv | Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability |
-
2002
- 2002-12-20 AT AT02805233T patent/ATE501167T1/de not_active IP Right Cessation
- 2002-12-20 DE DE60239420T patent/DE60239420D1/de not_active Expired - Lifetime
- 2002-12-20 JP JP2003554874A patent/JP4408371B2/ja not_active Expired - Fee Related
- 2002-12-20 CN CNB028281144A patent/CN100526455C/zh not_active Expired - Fee Related
- 2002-12-20 EP EP02805233A patent/EP1458852B1/de not_active Expired - Lifetime
- 2002-12-20 AU AU2002357410A patent/AU2002357410A1/en not_active Abandoned
- 2002-12-20 WO PCT/BE2002/000196 patent/WO2003054170A1/en active Application Filing
-
2005
- 2005-08-26 HK HK05107513.8A patent/HK1075276A1/xx not_active IP Right Cessation
-
2008
- 2008-11-20 US US12/274,939 patent/US8697444B2/en not_active Expired - Fee Related
-
2013
- 2013-09-19 US US14/031,592 patent/US8993323B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP4408371B2 (ja) | 2010-02-03 |
| US8697444B2 (en) | 2014-04-15 |
| AU2002357410A8 (en) | 2003-07-09 |
| WO2003054170A1 (en) | 2003-07-03 |
| WO2003054170A8 (en) | 2004-04-29 |
| EP1458852A1 (de) | 2004-09-22 |
| CN100526455C (zh) | 2009-08-12 |
| US20140099710A1 (en) | 2014-04-10 |
| JP2005512563A (ja) | 2005-05-12 |
| AU2002357410A1 (en) | 2003-07-09 |
| HK1075276A1 (en) | 2005-12-09 |
| ATE501167T1 (de) | 2011-03-15 |
| US20090098650A1 (en) | 2009-04-16 |
| US8993323B2 (en) | 2015-03-31 |
| CN1620498A (zh) | 2005-05-25 |
| EP1458852B1 (de) | 2011-03-09 |
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