HUP0402337A2 - Eljárás poxvírusok kinyerésére és tisztítására fertőzött sejtekből - Google Patents

Eljárás poxvírusok kinyerésére és tisztítására fertőzött sejtekből

Info

Publication number
HUP0402337A2
HUP0402337A2 HU0402337A HUP0402337A HUP0402337A2 HU P0402337 A2 HUP0402337 A2 HU P0402337A2 HU 0402337 A HU0402337 A HU 0402337A HU P0402337 A HUP0402337 A HU P0402337A HU P0402337 A2 HUP0402337 A2 HU P0402337A2
Authority
HU
Hungary
Prior art keywords
poxvirus
poxviruses
infected cells
purification
recovery
Prior art date
Application number
HU0402337A
Other languages
English (en)
Inventor
Karl Heller
Jutta Kramer
Original Assignee
Bavarian Nordic A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic A/S filed Critical Bavarian Nordic A/S
Publication of HUP0402337A2 publication Critical patent/HUP0402337A2/hu
Publication of HUP0402337A3 publication Critical patent/HUP0402337A3/hu
Publication of HU227507B1 publication Critical patent/HU227507B1/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

A találmány tárgyát eljárás képezi poxvírusok, közelebbről módosítottvacciniavírus Ankara (MVA) kinyerésére fertőzött sejtekből. Atalálmány szerinti eljárás szerint a poxvírusfertőzött sejteket nagynyomással homogenizálják, miáltal poxvírustartalmú homogenizátumotkapnak. A poxvírustartalmú homogenizátumot legalább egy tisztításilépésnek vetik alá, miáltal poxvírusokban dúsított frakciót nyernek.Ugyancsak a találmány tárgyát képezi a találmány szerinti eljárássalkapott poxvírustartalmú frakció és poxvírustartalmú homogenizátum. Ó
HU0402337A 2001-12-20 2002-12-13 Eljárás poxvírusok kinyerésére és tisztítására fertõzött sejtekbõl HU227507B1 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101928 2001-12-20
PCT/EP2002/014179 WO2003054175A1 (en) 2001-12-20 2002-12-13 Method for the recovery and purification of poxviruses from infected cells

Publications (3)

Publication Number Publication Date
HUP0402337A2 true HUP0402337A2 (hu) 2005-01-28
HUP0402337A3 HUP0402337A3 (en) 2010-01-28
HU227507B1 HU227507B1 (hu) 2011-07-28

Family

ID=8160920

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0402337A HU227507B1 (hu) 2001-12-20 2002-12-13 Eljárás poxvírusok kinyerésére és tisztítására fertõzött sejtekbõl

Country Status (23)

Country Link
US (1) US7056723B2 (hu)
EP (1) EP1407006B9 (hu)
JP (2) JP5322252B2 (hu)
KR (1) KR100947976B1 (hu)
CN (1) CN1289663C (hu)
AT (1) ATE302268T1 (hu)
AU (1) AU2002352247B2 (hu)
BR (1) BRPI0215003B1 (hu)
CA (1) CA2467099C (hu)
DE (1) DE60205631T2 (hu)
DK (1) DK1407006T3 (hu)
EA (1) EA006485B1 (hu)
ES (1) ES2247404T3 (hu)
HK (1) HK1071765A1 (hu)
HU (1) HU227507B1 (hu)
IL (2) IL161526A0 (hu)
MX (1) MXPA04006001A (hu)
NO (1) NO335975B1 (hu)
NZ (1) NZ533586A (hu)
PL (1) PL205929B1 (hu)
SI (1) SI1407006T1 (hu)
UA (1) UA77735C2 (hu)
WO (1) WO2003054175A1 (hu)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7445924B2 (en) 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
CN100537773C (zh) 2000-11-23 2009-09-09 巴法里安诺迪克有限公司 改良安卡拉痘苗病毒变体
ATE393212T2 (de) * 2002-09-05 2008-05-15 Bavarian Nordic As Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen
WO2006052826A2 (en) * 2004-11-05 2006-05-18 The Government Of The United States Of America As Represented By The Secretary Methods for preparing cells and viruses
US7625570B1 (en) * 2005-03-10 2009-12-01 The Regents Of The University Of California Methods for purifying adeno-associated virus
US8003363B2 (en) 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
AU2008250596C1 (en) * 2007-05-14 2010-11-25 Bavarian Nordic A/S Purification of Vaccinia virus- and recombinant Vaccinia virus-based vaccines
BRPI0908474A2 (pt) * 2008-02-12 2016-07-26 Sanofi Pasteur Ltd métodos de uso de cromatografia de troca de íon e de filtração de gel para purificação de vírus causador da catapora
RU2560976C2 (ru) * 2009-05-12 2015-08-20 Трансген Са Способ продуцирования и очистки ортопоксвируса
FR2953686B1 (fr) * 2009-12-14 2012-11-02 Agronomique Inst Nat Rech Procede pour reduire la teneur bacterienne d'un milieu alimentaire et/ou biologique d'interet, contenant des gouttelettes lipidiques
AU2011281982B2 (en) 2010-07-20 2015-12-17 Bavarian Nordic A/S Method for harvesting expression products
CN102353792A (zh) * 2011-07-07 2012-02-15 贵州大学 一种基于gpv p32蛋白的间接elisa试剂盒及制备方法
FR3042121A1 (fr) 2015-10-08 2017-04-14 Jean-Marc Limacher Composition anti-tumorale
CA3062549A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
CA3061678A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
WO2020049151A1 (en) 2018-09-06 2020-03-12 Bavarian Nordic A/S Storage improved poxvirus compositions
BR112022017438A2 (pt) 2020-03-12 2022-10-18 Bavarian Nordic As Composições que melhoram a estabilidade do poxvírus
WO2024003239A1 (en) 2022-06-29 2024-01-04 Bavarian Nordic A/S RECOMBINANT MODIFIED saRNA (VRP) AND VACCINIA VIRUS ANKARA (MVA) PRIME-BOOST REGIMEN
WO2024003238A1 (en) 2022-06-29 2024-01-04 Bavarian Nordic A/S Epstein-barr-virus vaccine
CN115197966A (zh) * 2022-08-15 2022-10-18 深圳源兴基因技术有限公司 一种利用生物反应器大规模生产重组痘苗病毒载体的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB877898A (en) 1956-12-06 1961-09-20 Apv Co Ltd A new or improved method of liberating enzymes
JPS5328989B2 (hu) 1974-05-27 1978-08-17
CH621573A5 (en) 1976-08-05 1981-02-13 Vnii Biosinteza Belkovykh Vesc Process for the continuous disintegration of microorganism cells and system for carrying out this process
JPS54154594A (en) 1978-05-25 1979-12-05 Sumitomo Chem Co Ltd Extraction of glucose isomerase
WO1991001367A1 (en) 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
UA68327C2 (en) * 1995-07-04 2004-08-16 Gsf Forschungszentrum Fur Unwe A recombinant mva virus, an isolated eukaryotic cell, infected with recombinant mva virus, a method for production in vitro of polypeptides with use of said cell, a method for production in vitro of virus parts (variants), vaccine containing the recombinant mva virus, a method for immunization of animals
TW426733B (en) 1995-10-06 2001-03-21 Merck & Co Inc Method of disrupting cultured cells using an impinging jet device
ID19548A (id) * 1996-09-24 1998-07-23 Bavarian Nordic Res Inst As Virus mva rekombinan yang mengekspresikan antigen-antigen virus demam dan penggunaan daripadanya dalam vaksin-vaksin
US5721120A (en) 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
NZ299720A (en) * 1996-11-08 1999-07-29 Howick Engineering Ltd Epicyclic input/output annuli of same pcd, with planetary input/output gear sets, and with only one set having aligned teeth
EP0968284B1 (en) * 1996-11-20 2006-12-13 Introgen Therapeutics, Inc. An improved method for the production and purification of adenoviral vectors
US6000551A (en) 1996-12-20 1999-12-14 Eastman Chemical Company Method for rupturing microalgae cells
FR2787465A1 (fr) * 1998-12-21 2000-06-23 Transgene Sa Procede d'inactivation des virus enveloppes dans une preparation virale de virus non enveloppes
US7264958B1 (en) * 1999-02-22 2007-09-04 Transgene, S.A. Method for obtaining a purified viral preparation
DE19918619A1 (de) 1999-04-23 2000-10-26 Rhein Biotech Proz & Prod Gmbh Verfahren zum Gewinnen von rekombinantem HBsAg
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen

Also Published As

Publication number Publication date
EP1407006A1 (en) 2004-04-14
US20040265986A1 (en) 2004-12-30
MXPA04006001A (es) 2005-08-19
EP1407006B9 (en) 2006-10-18
HU227507B1 (hu) 2011-07-28
KR100947976B1 (ko) 2010-03-15
DK1407006T3 (da) 2005-12-12
ATE302268T1 (de) 2005-09-15
CA2467099A1 (en) 2003-07-03
IL161526A0 (en) 2004-09-27
HUP0402337A3 (en) 2010-01-28
AU2002352247B2 (en) 2008-08-07
CA2467099C (en) 2013-01-29
HK1071765A1 (en) 2005-07-29
EA200400833A1 (ru) 2004-12-30
BRPI0215003B1 (pt) 2017-03-14
AU2002352247A1 (en) 2003-07-09
KR20040070263A (ko) 2004-08-06
DE60205631T2 (de) 2006-06-08
CN1289663C (zh) 2006-12-13
NZ533586A (en) 2005-02-25
PL369498A1 (en) 2005-04-18
JP5322252B2 (ja) 2013-10-23
EP1407006B1 (en) 2005-08-17
NO335975B1 (no) 2015-04-07
EA006485B1 (ru) 2005-12-29
SI1407006T1 (sl) 2006-04-30
PL205929B1 (pl) 2010-06-30
US7056723B2 (en) 2006-06-06
UA77735C2 (en) 2007-01-15
CN1606616A (zh) 2005-04-13
IL161526A (en) 2009-11-18
JP2010046093A (ja) 2010-03-04
NO20043181L (no) 2004-07-19
DE60205631D1 (de) 2005-09-22
BR0215003A (pt) 2004-11-09
ES2247404T3 (es) 2006-03-01
WO2003054175A1 (en) 2003-07-03
JP2005512565A (ja) 2005-05-12

Similar Documents

Publication Publication Date Title
HUP0402337A2 (hu) Eljárás poxvírusok kinyerésére és tisztítására fertőzött sejtekből
WO2005038051A3 (en) Improved bisulfite conversion of dna
DE60221530D1 (de) Verfahren und vorrichtung zum unterdrücken von tönen, die durch dem-algorithmen (cyclic dynamic element matching) verursacht werden
DE60035418D1 (de) Wasserstoff-durchlässige metallmembran und verfahren zu ihrer herstellung
MY140734A (en) Thermal separating process for removing at least one stream containing enriched (meth) acrylic monomers
ATE521627T1 (de) Identifikation kreuzreaktiver epitope des flavivirus-hüllglykoproteins
WO2002074978A3 (en) Nucleic acid shuffling
MX2007004696A (es) Metodos de purificacion viral mejorados.
DK1449425T3 (da) Fremgangsmåde og apparat til opdeling af en höstmaterialeström
NO20051138L (no) Nye farmasoytiske formuleringer av modafinil
WO2003072034A3 (en) Cyclic peptides and analogs useful to treat allergies
CY1107632T1 (el) Διαδικασια για την παρασκευη μιας θρομβινης στην οποια εχουν αδρανοποιηθει οι ιοι
ATE555605T1 (de) Verfahren der bitstream-filterung gemäss benutzerspezifikationen
ATE399210T1 (de) Knockout-mutante von vaccinia-virus und verwendung davon
NO20062436L (no) Fremgangsmate og system for fjerning av kontaminanter fra en naturgasstrom
MXPA03007535A (es) Metodo para extraer virus de cultivo celular.
DK1508047T3 (da) Fremgangsmåde til identifikation af immunoreaktive peptider
TW200517121A (en) Immunogen
NO20022182D0 (no) Fremgangsmåte og innretning for utnyttelse av reaksjonsvarme som dannes ved fremstilling av 1,2-dikloretan
NO20013747D0 (no) Fremgangsmåte ved gjenvinning av VOC-gass og anlegg for gjenvinning av VOC-gass
WO2002072803A3 (en) Subgenomic replicons of the flavivirus dengue
DE60206091D1 (de) Methoden zum Betreiben von Gasturbinen
WO2002012471A3 (en) Angiotensin converting enzyme homolog and uses therefor
DE60223142D1 (de) Verfahren zur bearbeitung von ginseng sowie bearbeiteter ginseng erhaeltlich durch dieses verfahren
NO20044580L (no) Dobbel refluksprosess samt konfigurasjoner for forbedret naturgassveskegjenvinning

Legal Events

Date Code Title Description
MM4A Lapse of definitive patent protection due to non-payment of fees